Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 551,602 A→T 33.8% F136L (TTT→TTA)  ybcI ← DUF457 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273551,6020AT33.8% 249.6 / 214.2 201F136L (TTT→TTA) ybcIDUF457 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (77/56);  new base T (44/24);  total (121/80)
Fisher's exact test for biased strand distribution p-value = 3.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 202 total aligned reads displayed.

CGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGA  >  CP009273/551456‑551749
                                                                                                                                                  |                                                                                                                                                   
 gcTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAAc                                                                                                                                                  <  1:3790445/149‑1 (MQ=255)
   tATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACgg                                                                                                                                                >  1:829355/1‑149 (MQ=255)
   tATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACgg                                                                                                                                                >  1:4087469/1‑149 (MQ=255)
    aTTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGgc                                                                                                                                               >  2:249338/1‑149 (MQ=255)
         cGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGAc                                                                                                                                          <  2:3585690/149‑1 (MQ=255)
         cGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACAGTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGAc                                                                                                                                          <  2:3842796/149‑1 (MQ=255)
             tGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGAATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACtttt                                                                                                                                      >  1:3010522/1‑149 (MQ=255)
              gCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTa                                                                                                                                     >  1:2856478/1‑149 (MQ=255)
                gAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATc                                                                                                                                   >  1:1035126/1‑149 (MQ=255)
                  gTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCAc                                                                                                                                 >  1:875338/1‑149 (MQ=255)
                   ttttCGCATCCGGTTATCGTCTTCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCAcc                                                                                                                                >  1:2719743/1‑149 (MQ=255)
                     ttCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTg                                                                                                                              <  1:1211208/149‑1 (MQ=255)
                         cATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAg                                                                                                                          >  2:3471490/1‑149 (MQ=255)
                         cATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAg                                                                                                                          >  1:2638947/1‑149 (MQ=255)
                          aTCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAgg                                                                                                                         >  1:2924559/1‑149 (MQ=255)
                          aTCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAgg                                                                                                                         >  2:2411376/1‑149 (MQ=255)
                          aTCCGGTTATCGTCTGCACCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAgg                                                                                                                         <  1:792538/149‑1 (MQ=255)
                             cGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTATCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAg                                                                                                                      <  2:2730158/149‑1 (MQ=255)
                              ggTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGc                                                                                                                     <  2:3575134/149‑1 (MQ=255)
                              ggTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGc                                                                                                                     <  2:3941471/149‑1 (MQ=255)
                              ggTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGc                                                                                                                     <  2:2770376/149‑1 (MQ=255)
                              ggTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGc                                                                                                                     <  2:3343587/149‑1 (MQ=255)
                                ttATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGa                                                                                                                   >  1:2405656/1‑149 (MQ=255)
                                   tCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGaaaa                                                                                                                >  2:31426/1‑149 (MQ=255)
                                   tCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGaaaa                                                                                                                >  2:3070436/1‑149 (MQ=255)
                                       cTGCTCCACCACAACATTCCAATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGACACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCg                                                                                                            <  1:496056/149‑1 (MQ=255)
                                           tccaccaCAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCa                                                                                                        >  1:493280/2‑149 (MQ=255)
                                                cacaACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGa                                                                                                   <  2:2670105/149‑1 (MQ=255)
                                                cacaACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGa                                                                                                   >  2:971591/1‑149 (MQ=255)
                                                   aacaTGCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAAGTCAGAAATAAACACCTGATGCCCGTACGGGGTGGTGTAACGACACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCa                                                                                                <  1:1762592/149‑1 (MQ=255)
                                                      aTTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACgg                                                                                             >  1:636096/1‑149 (MQ=255)
                                                       ttCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGc                                                                                            >  2:700327/1‑149 (MQ=255)
                                                         cccATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCca                                                                                          >  1:1494425/1‑149 (MQ=255)
                                                         cccATCAGTAGCATCCCCGCCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCca                                                                                          <  1:3609202/149‑1 (MQ=255)
                                                            aTCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCAcag                                                                                       >  1:622479/1‑149 (MQ=255)
                                                            aTCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCAcag                                                                                       >  1:3757893/1‑149 (MQ=255)
                                                            aTCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCAcag                                                                                       >  2:2867845/1‑149 (MQ=255)
                                                             tCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCAcagc                                                                                      >  2:1907428/1‑149 (MQ=255)
                                                             tCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCAcagc                                                                                      >  2:3946196/1‑149 (MQ=255)
                                                              cAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCAcagcc                                                                                     <  1:2327003/149‑1 (MQ=255)
                                                              cAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCAcagcc                                                                                     <  2:2034144/149‑1 (MQ=255)
                                                              cAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCAcagcc                                                                                     <  2:3893729/149‑1 (MQ=255)
                                                               aGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCAcagcca                                                                                    <  2:3017408/149‑1 (MQ=255)
                                                               aGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCAcagcca                                                                                    <  2:3198794/149‑1 (MQ=255)
                                                                   gCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAgccagcca                                                                                >  1:2729073/1‑149 (MQ=255)
                                                                     aTCCCCGGCAACCACACCCACATCAATTCAGAATTAATCACCTGATGCCCGTACGGCTTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAAc                                                                              <  1:1309154/149‑1 (MQ=255)
                                                                      tCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACg                                                                             >  1:3440034/1‑149 (MQ=255)
                                                                        cccGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGcc                                                                           >  1:3144584/1‑149 (MQ=255)
                                                                        cccGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGcc                                                                           >  1:3142233/1‑149 (MQ=255)
                                                                        cccGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGcc                                                                           >  2:2371890/1‑149 (MQ=255)
                                                                            gCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTa                                                                       <  2:923718/149‑1 (MQ=255)
                                                                             cAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTAc                                                                      <  2:3144584/149‑1 (MQ=255)
                                                                             cAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTAc                                                                      <  1:3366791/149‑1 (MQ=255)
                                                                                ccACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGc                                                                   >  1:2434539/1‑149 (MQ=255)
                                                                                ccACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGc                                                                   >  2:400765/1‑149 (MQ=255)
                                                                                  acacCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCa                                                                 >  1:3307259/1‑149 (MQ=255)
                                                                                    acCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGt                                                               >  1:364118/1‑149 (MQ=255)
                                                                                      ccACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTgg                                                             >  2:476898/1‑149 (MQ=255)
                                                                                       cacaTCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGt                                                            <  2:2143566/149‑1 (MQ=255)
                                                                                          aTCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTAc                                                         <  2:1837649/149‑1 (MQ=255)
                                                                                           tCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTAcc                                                        >  1:1996148/1‑149 (MQ=255)
                                                                                             aaTTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGa                                                      >  1:2770028/1‑149 (MQ=255)
                                                                                             aaTTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGa                                                      >  1:3048606/1‑149 (MQ=255)
                                                                                             aaTTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGa                                                      <  2:2158524/149‑1 (MQ=255)
                                                                                                 cAGAAATAATCACCTTATGCCTTTACGGTGTGGTGAAACGAGAAAATGAAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATcc                                                  <  1:3896052/149‑1 (MQ=255)
                                                                                                 cAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATcc                                                  <  1:971591/149‑1 (MQ=255)
                                                                                                  aGAAATAATCCCCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCa                                                 <  2:1418920/149‑1 (MQ=255)
                                                                                                  aGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCa                                                 >  1:1097063/1‑149 (MQ=255)
                                                                                                      ataatCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCaa                                             >  1:669973/1‑149 (MQ=255)
                                                                                                       taatCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAg                                            >  1:4036606/1‑149 (MQ=255)
                                                                                                        aatCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGc                                           >  1:487451/1‑149 (MQ=255)
                                                                                                         atCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCt                                          >  1:3936198/1‑149 (MQ=255)
                                                                                                          tCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCtg                                         >  2:1382298/1‑149 (MQ=255)
                                                                                                             ccTGATGCCCGTACGGCGTGGTGTAACGAGACAAGGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGc                                      <  1:22551/149‑1 (MQ=255)
                                                                                                              cTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCg                                     <  1:2800089/149‑1 (MQ=255)
                                                                                                               tGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGa                                    <  1:1723159/149‑1 (MQ=255)
                                                                                                                gATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGAt                                   >  2:3629555/1‑149 (MQ=255)
                                                                                                                 aTGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATa                                  >  1:1659445/1‑149 (MQ=255)
                                                                                                                     ccGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCaa                              <  1:2222371/149‑1 (MQ=255)
                                                                                                                      cGTACGGCGTGGTGTAACGAGACAATGCACACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAAc                             <  1:1814225/149‑1 (MQ=255)
                                                                                                                      cGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAAc                             >  2:1324590/1‑149 (MQ=255)
                                                                                                                       gTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACg                            >  1:1582099/1‑149 (MQ=255)
                                                                                                                           ggCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGAc                        <  2:4061078/149‑1 (MQ=255)
                                                                                                                           ggCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGAc                        <  2:3802392/149‑1 (MQ=255)
                                                                                                                             cGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACgg                      <  2:651891/149‑1 (MQ=255)
                                                                                                                             cGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACgg                      <  1:249338/149‑1 (MQ=255)
                                                                                                                              gtggtgTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGAAACGAGACGGt                     >  1:3386492/1‑149 (MQ=255)
                                                                                                                                 gtgtAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTaa                  >  1:1591297/1‑149 (MQ=255)
                                                                                                                                  tgtAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTaaa                 <  1:3270476/149‑1 (MQ=255)
                                                                                                                                  tgtAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTaaa                 <  1:3750206/149‑1 (MQ=255)
                                                                                                                                   gtAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTaaaa                <  2:1057002/149‑1 (MQ=255)
                                                                                                                                     aaCGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAAc              >  2:355617/1‑149 (MQ=255)
                                                                                                                                       cGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAAcag            >  1:1477435/1‑149 (MQ=255)
                                                                                                                                         agaCAATGAAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAAcagcc          <  1:1375612/149‑1 (MQ=255)
                                                                                                                                            cAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAAcagccagc       >  1:3467576/1‑149 (MQ=255)
                                                                                                                                             aaTGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCcagca      <  2:1611941/149‑1 (MQ=255)
                                                                                                                                              aTGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCcagcag     >  2:1209116/1‑149 (MQ=255)
                                                                                                                                               tGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCagcagc    >  1:25234/1‑149 (MQ=255)
                                                                                                                                               tGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCagcagc    >  2:770530/1‑149 (MQ=255)
                                                                                                                                                 cAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGa  <  1:629990/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGCGA  >  CP009273/551456‑551749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑