Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 591,864 C→A 100% A270E (GCG→GAG)  cusC → copper/silver efflux system, outer membrane component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273591,8640CA98.6% 864.1 / 2.7 282A270E (GCG→GAG) cusCcopper/silver efflux system, outer membrane component
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (113/164);  total (115/167)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.81e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 285 total aligned reads displayed.

AGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTA  >  CP009273/591718‑592010
                                                                                                                                                  |                                                                                                                                                  
 gAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAggg                                                                                                                                                 >  1:1054080/1‑149 (MQ=255)
 gAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGAAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAggg                                                                                                                                                 <  1:3945838/149‑1 (MQ=255)
  aaaGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGa                                                                                                                                                <  1:993055/149‑1 (MQ=255)
   aaGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGAt                                                                                                                                               <  2:4032665/149‑1 (MQ=255)
   aaGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGAt                                                                                                                                               <  1:2753189/149‑1 (MQ=255)
   aaGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGAt                                                                                                                                               <  2:1248917/149‑1 (MQ=255)
   aaGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGAt                                                                                                                                               <  2:2001062/149‑1 (MQ=255)
     gTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTg                                                                                                                                             <  1:1693383/149‑1 (MQ=255)
      tACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTgt                                                                                                                                            <  2:790930/149‑1 (MQ=255)
       aCCCGCAGCGACCTCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTgtc                                                                                                                                           <  2:3424809/149‑1 (MQ=255)
       aCCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTgtc                                                                                                                                           >  2:2955262/1‑149 (MQ=255)
        cccGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTgtcg                                                                                                                                          <  1:898047/149‑1 (MQ=255)
        cccGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTgtcg                                                                                                                                          <  1:2960331/149‑1 (MQ=255)
              gCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCaa                                                                                                                                    >  2:3471179/1‑149 (MQ=255)
                gACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAAt                                                                                                                                  >  1:243985/1‑149 (MQ=255)
                 aCATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATc                                                                                                                                 >  2:3114370/1‑149 (MQ=255)
                  cATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCt                                                                                                                                >  1:2326573/1‑149 (MQ=255)
                     cGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTAt                                                                                                                             >  1:3513383/1‑149 (MQ=255)
                        tAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGc                                                                                                                          >  2:2154507/1‑149 (MQ=255)
                         aaaCGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCa                                                                                                                         <  1:3285208/149‑1 (MQ=255)
                         aaaCGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCa                                                                                                                         <  1:1606404/149‑1 (MQ=255)
                         aaaCGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCa                                                                                                                         >  1:444474/1‑149 (MQ=255)
                             gTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAgcgc                                                                                                                     <  1:3179343/149‑1 (MQ=255)
                              tCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGcc                                                                                                                    >  2:2259708/1‑149 (MQ=255)
                                 gggggAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCgg                                                                                                                 >  2:3906611/1‑149 (MQ=255)
                                     gAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCACGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGatat                                                                                                             <  1:1863207/149‑1 (MQ=255)
                                     gAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGatat                                                                                                             <  1:3433763/149‑1 (MQ=255)
                                       aCTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGAtatta                                                                                                           >  1:3724300/1‑149 (MQ=255)
                                       aCTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGAtatta                                                                                                           >  2:3346960/1‑149 (MQ=255)
                                        cTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGAtattat                                                                                                          <  2:2565869/149‑1 (MQ=255)
                                         tgccgcCGGCCAATAACCCAGTCCAACTGTGGCTGGGACCCTACGGCAACCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATg                                                                                                         <  1:874759/146‑1 (MQ=255)
                                         tGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATg                                                                                                         >  1:2359047/1‑149 (MQ=255)
                                          ggCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATgg                                                                                                        >  2:914355/1‑149 (MQ=255)
                                              cAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGc                                                                                                    <  1:4096413/149‑1 (MQ=255)
                                              cAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGc                                                                                                    <  1:1958174/149‑1 (MQ=255)
                                               aGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCt                                                                                                   >  1:2677234/1‑149 (MQ=255)
                                                ggCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTg                                                                                                  <  2:1857789/149‑1 (MQ=255)
                                                ggCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTg                                                                                                  >  2:95411/1‑149 (MQ=255)
                                                    aataatGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAaca                                                                                              <  1:721152/149‑1 (MQ=255)
                                                      taatGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACAcg                                                                                            >  2:2893406/1‑149 (MQ=255)
                                                         tGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGt                                                                                         <  2:1084645/149‑1 (MQ=255)
                                                          gCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGtt                                                                                        <  2:3957173/149‑1 (MQ=255)
                                                            aTTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTaa                                                                                      <  1:3137937/149‑1 (MQ=255)
                                                             ttGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAAt                                                                                     <  2:1656859/149‑1 (MQ=255)
                                                             ttGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAAt                                                                                     >  1:615767/1‑149 (MQ=255)
                                                              tGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATg                                                                                    <  2:2972622/149‑1 (MQ=255)
                                                               gCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATgg                                                                                   <  2:3837863/149‑1 (MQ=255)
                                                                cAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATggc                                                                                  >  2:594256/1‑149 (MQ=255)
                                                                   cTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACATCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATggcggc                                                                               >  2:4085814/1‑149 (MQ=255)
                                                                   cTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATggcggc                                                                               >  2:2638411/1‑149 (MQ=255)
                                                                    tGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCt                                                                              >  1:3576804/1‑149 (MQ=255)
                                                                    tGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCt                                                                              <  1:212483/149‑1 (MQ=255)
                                                                     gTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACATTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTa                                                                             <  1:1150061/149‑1 (MQ=255)
                                                                      ttGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACTGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTaa                                                                            <  2:1117785/149‑1 (MQ=255)
                                                                       tGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAAt                                                                           <  1:3051439/149‑1 (MQ=255)
                                                                         cTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGc                                                                         <  1:2834896/149‑1 (MQ=255)
                                                                          tGGGAAGCTACGGCAAGCTGCCGCACGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGcc                                                                        <  2:2769603/149‑1 (MQ=255)
                                                                          tGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGcc                                                                        <  1:1430520/149‑1 (MQ=255)
                                                                          tGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGcc                                                                        <  1:452182/149‑1 (MQ=255)
                                                                           gggAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCa                                                                       >  2:2523094/1‑149 (MQ=255)
                                                                            ggAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCaa                                                                      >  2:932006/1‑149 (MQ=255)
                                                                             gAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAat                                                                     <  1:2554333/149‑1 (MQ=255)
                                                                                  tACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTgg                                                                >  2:879456/1‑149 (MQ=255)
                                                                                   aCGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGgc                                                               >  1:384617/1‑149 (MQ=255)
                                                                                     ggCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGgcgc                                                             <  2:3786867/149‑1 (MQ=255)
                                                                                     ggCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGgcgc                                                             >  2:678457/1‑149 (MQ=255)
                                                                                      gCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCt                                                            >  1:2627190/1‑149 (MQ=255)
                                                                                        aaGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGc                                                          >  1:218679/1‑149 (MQ=255)
                                                                                        aaGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGc                                                          >  2:144788/1‑149 (MQ=255)
                                                                                                   aGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCAt                                               >  2:174700/1‑149 (MQ=255)
                                                                                                   aGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCAt                                               <  2:482152/149‑1 (MQ=255)
                                                                                                    gcgcAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCAtt                                              >  1:2213681/1‑149 (MQ=255)
                                                                                                      gcAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCAtttt                                            >  2:1347596/1‑149 (MQ=255)
                                                                                                      gcAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCAtttt                                            <  1:676311/149‑1 (MQ=255)
                                                                                                        aGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCAtttttt                                          <  2:1724940/149‑1 (MQ=255)
                                                                                                        aGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCAtttttt                                          <  1:95411/149‑1 (MQ=255)
                                                                                                             gTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTc                                     >  1:2072935/1‑149 (MQ=255)
                                                                                                             gTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTc                                     >  2:3590571/1‑149 (MQ=255)
                                                                                                                 aCAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCtata                                 <  2:3782162/149‑1 (MQ=255)
                                                                                                                  cAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATaa                                <  1:1314040/149‑1 (MQ=255)
                                                                                                                      gACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCt                            <  1:1184529/149‑1 (MQ=255)
                                                                                                                        cAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGa                          >  1:3983261/1‑149 (MQ=255)
                                                                                                                         aGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGAc                         >  1:201284/1‑149 (MQ=255)
                                                                                                                         aGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTAGGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGTCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGAc                         >  1:2274618/1‑149 (MQ=255)
                                                                                                                          gCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGAcc                        <  2:1375196/149‑1 (MQ=255)
                                                                                                                             tGCAAATCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGATAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGc                     <  1:2601203/149‑1 (MQ=255)
                                                                                                                              gCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCg                    >  2:2085541/1‑149 (MQ=255)
                                                                                                                              gCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCg                    <  2:3625597/149‑1 (MQ=255)
                                                                                                                               cAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCgg                   <  1:627922/149‑1 (MQ=255)
                                                                                                                                 aaGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGaa                 >  2:3736914/1‑149 (MQ=255)
                                                                                                                                 aaGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGaa                 >  1:2518215/1‑149 (MQ=255)
                                                                                                                                   gCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGaaca               <  1:2143977/149‑3 (MQ=255)
                                                                                                                                        aaaTTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGACATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGAc          <  1:148218/149‑1 (MQ=255)
                                                                                                                                         aaTTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGAcc         <  2:3513656/149‑1 (MQ=255)
                                                                                                                                         aaTTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGAcc         >  1:4018808/1‑149 (MQ=255)
                                                                                                                                         aaTTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGAcc         <  2:2729105/149‑1 (MQ=255)
                                                                                                                                          aTTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCg        >  1:1676072/1‑149 (MQ=255)
                                                                                                                                             aCCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCa     <  1:3783668/149‑1 (MQ=255)
                                                                                                                                              ccGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAg    <  2:3246357/149‑1 (MQ=255)
                                                                                                                                                ggcggGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTa  <  1:3471179/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTA  >  CP009273/591718‑592010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑