Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 647,234 C→T 94.6% E27K (GAA→AAA)  citC ← [citrate [pro‑3S]‑lyase] ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273647,2340CT94.6% 716.9 / 24.3 224E27K (GAA→AAA) citC[citrate [pro‑3S]‑lyase] ligase
Reads supporting (aligned to +/- strand):  ref base C (4/8);  new base T (106/105);  total (110/114)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 224 total aligned reads displayed.

GCGCCAGTCCTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTCATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAA  >  CP009273/647090‑647378
                                                                                                                                                |                                                                                                                                                
gcgcCAGTCCTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGc                                                                                                                                              >  2:10899/1‑149 (MQ=255)
    cAGTCCTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGa                                                                                                                                          >  1:1878885/1‑149 (MQ=255)
      gTCCTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAAt                                                                                                                                        <  1:463645/149‑1 (MQ=255)
       tCCTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAAtt                                                                                                                                       >  2:1036950/1‑149 (MQ=255)
       tCCTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAAtt                                                                                                                                       <  2:3578550/149‑1 (MQ=255)
       tCCTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAAtt                                                                                                                                       >  2:403920/1‑149 (MQ=255)
         cTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTgg                                                                                                                                     <  1:1582656/149‑1 (MQ=255)
          ttCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTggg                                                                                                                                    >  1:3869449/1‑149 (MQ=255)
           tCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGc                                                                                                                                   <  2:866278/149‑1 (MQ=255)
           tCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGc                                                                                                                                   <  1:2888750/149‑1 (MQ=255)
             aCCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGGTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTGTTATGCAGGAATTGGGCGa                                                                                                                                 >  2:3185801/1‑149 (MQ=255)
               cgcgGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGAtt                                                                                                                               <  1:3370025/149‑1 (MQ=255)
                 cggacggaTTCACTGATAGCAACGCATTTAATAATATTTCCGGCACTTCCACCGCACGCGATAAGCTTTTCTTCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTc                                                                                                                             <  1:4103846/149‑1 (MQ=255)
                 cggacggaTTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTc                                                                                                                             <  1:4102971/149‑1 (MQ=255)
                 cggacggaTTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTc                                                                                                                             <  1:504101/149‑1 (MQ=255)
                   gacggaTTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCg                                                                                                                           >  2:890314/1‑149 (MQ=255)
                     cggaTTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGcc                                                                                                                         >  1:3600665/1‑149 (MQ=255)
                      ggaTTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCa                                                                                                                        <  1:1208456/149‑1 (MQ=255)
                          tCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCAtttt                                                                                                                    >  2:2818749/1‑149 (MQ=255)
                           cACTGATAGCAACGCATTAAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTCATGCAGGAATTGGGCGATTTCCGCCAttttt                                                                                                                   <  1:1476657/149‑1 (MQ=255)
                              tGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTa                                                                                                                >  2:3153170/1‑149 (MQ=255)
                              tGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTa                                                                                                                <  1:3946408/149‑1 (MQ=255)
                               gATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTAt                                                                                                               <  2:2033872/149‑1 (MQ=255)
                                atcgCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTAtt                                                                                                              <  2:143028/146‑1 (MQ=255)
                                aTAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTAtt                                                                                                              >  1:3523609/1‑149 (MQ=255)
                                 tAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTAttt                                                                                                             <  2:1120485/149‑1 (MQ=255)
                                 tAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTAttt                                                                                                             <  1:1371510/149‑1 (MQ=255)
                                  aGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTAtttt                                                                                                            <  1:1278725/149‑1 (MQ=255)
                                  aGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTAtttt                                                                                                            <  1:3193759/149‑1 (MQ=255)
                                  aGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTAtttt                                                                                                            <  2:208310/149‑1 (MQ=255)
                                   gCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTAttttn                                                                                                           <  1:2459576/149‑2 (MQ=255)
                                   gCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTc                                                                                                           <  2:1127971/149‑1 (MQ=255)
                                   gCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTc                                                                                                           <  2:2143423/149‑1 (MQ=255)
                                    cAACGGATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCt                                                                                                          >  1:35925/1‑149 (MQ=255)
                                    cAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCt                                                                                                          <  1:345459/149‑1 (MQ=255)
                                      aCGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTCATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGa                                                                                                        >  1:1726017/1‑149 (MQ=255)
                                       cGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTCATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGaa                                                                                                       <  1:2562822/149‑1 (MQ=255)
                                        gCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAAc                                                                                                      >  1:752939/1‑149 (MQ=255)
                                         cATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACg                                                                                                     >  2:3496474/1‑149 (MQ=255)
                                         cATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACg                                                                                                     >  2:2205657/1‑149 (MQ=255)
                                         cATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACg                                                                                                     >  2:460912/1‑149 (MQ=255)
                                         cATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACg                                                                                                     >  2:128838/1‑149 (MQ=255)
                                          aTTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGt                                                                                                    <  1:2808538/149‑1 (MQ=255)
                                          aTTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGt                                                                                                    >  2:882479/1‑149 (MQ=255)
                                           tttAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGtt                                                                                                   >  2:2656491/1‑149 (MQ=255)
                                              aataatATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTa                                                                                                <  1:2333366/149‑1 (MQ=255)
                                                 aataTTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTAcgc                                                                                             <  2:2300341/149‑1 (MQ=255)
                                                   tatTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTAAAAAAAATCTGAACGTTTTACGCgg                                                                                           >  2:1388031/1‑149 (MQ=255)
                                                           gCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAat                                                                                   >  2:1266252/1‑149 (MQ=255)
                                                             aaTTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAatat                                                                                 >  2:1444584/1‑149 (MQ=255)
                                                               ttCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCa                                                                               <  2:1705990/149‑1 (MQ=255)
                                                                tCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCAt                                                                              <  2:2001924/149‑1 (MQ=255)
                                                                 ccACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCAtt                                                                             <  1:1448586/149‑1 (MQ=255)
                                                                  cACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTg                                                                            >  2:630973/1‑149 (MQ=255)
                                                                   aCCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGc                                                                           <  2:542488/149‑1 (MQ=255)
                                                                   aCCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATAATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGc                                                                           <  1:2818749/149‑1 (MQ=255)
                                                                    ccGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGcc                                                                          <  2:741015/149‑1 (MQ=255)
                                                                          gcgATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACat                                                                    <  2:166110/149‑1 (MQ=255)
                                                                            gATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACataa                                                                  >  1:1098847/1‑149 (MQ=255)
                                                                            gATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACataa                                                                  >  1:2669689/1‑149 (MQ=255)
                                                                             aTAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACataat                                                                 <  1:4084057/149‑1 (MQ=255)
                                                                               aaGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACAtaataa                                                               >  1:369059/1‑149 (MQ=255)
                                                                                aGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATaataaa                                                              >  2:3679421/1‑149 (MQ=255)
                                                                                 gCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATaataaat                                                             <  1:1084403/149‑1 (MQ=255)
                                                                                  cTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATaataaata                                                            <  1:3798043/149‑1 (MQ=255)
                                                                                   ttttCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAg                                                           >  1:1623027/1‑149 (MQ=255)
                                                                                    tttCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGt                                                          >  1:1718958/1‑149 (MQ=255)
                                                                                         tCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCt                                                     <  1:1639271/149‑1 (MQ=255)
                                                                                            cgGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGaa                                                  >  2:3708956/1‑149 (MQ=255)
                                                                                             gggTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAg                                                 >  1:22950/1‑149 (MQ=255)
                                                                                                ttACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTg                                              <  1:604857/149‑1 (MQ=255)
                                                                                                ttACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTg                                              <  1:1745517/149‑1 (MQ=255)
                                                                                                 tACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGc                                             <  2:3869449/149‑1 (MQ=255)
                                                                                                   cGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCAt                                           <  2:35925/149‑1 (MQ=255)
                                                                                                       aataaataCTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgttg                                       <  2:3105992/149‑1 (MQ=255)
                                                                                                       aataaataCTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgttg                                       <  2:3107553/149‑1 (MQ=255)
                                                                                                        ataaataCTTCGAATGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCGATTTTTTTATTTTCTGAACGTTTTACGCGGTTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgttgt                                      <  2:1876077/149‑1 (MQ=255)
                                                                                                         taaataCTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATgttgtt                                     >  2:2554761/1‑149 (MQ=255)
                                                                                                          aaataCTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTa                                    <  2:1936789/149‑1 (MQ=255)
                                                                                                           aataCTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTAt                                   <  2:3270468/149‑1 (MQ=255)
                                                                                                            ataCTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATc                                  >  1:3106196/1‑149 (MQ=255)
                                                                                                              aCTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGa                                >  2:3940248/1‑149 (MQ=255)
                                                                                                                ttCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGAtt                              <  1:1653286/149‑1 (MQ=255)
                                                                                                                  cGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTg                            >  2:796119/1‑149 (MQ=255)
                                                                                                                      tgtgGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAAc                        >  1:1266955/1‑149 (MQ=255)
                                                                                                                        tggtgTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGa                      >  2:3781231/1‑149 (MQ=255)
                                                                                                                         ggtgTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGaa                     >  1:1926074/1‑149 (MQ=255)
                                                                                                                          gtgtCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAAt                    >  2:311758/1‑149 (MQ=255)
                                                                                                                            gtCAACGCTCAAATCATTTTCATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAAtgt                  >  1:275134/1‑149 (MQ=255)
                                                                                                                              cAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAAtgttg                >  1:213401/1‑149 (MQ=255)
                                                                                                                                aCGCTCAAATCTTTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCAGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATgttgtt              <  2:2433694/149‑1 (MQ=255)
                                                                                                                                 cGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTc             >  1:861809/1‑149 (MQ=255)
                                                                                                                                   cTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCaa           >  2:3559810/1‑149 (MQ=255)
                                                                                                                                    tCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAAt          >  1:3819066/1‑149 (MQ=255)
                                                                                                                                     cAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATg         <  2:3600665/149‑1 (MQ=255)
                                                                                                                                       aaTCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGtt       <  1:444629/149‑1 (MQ=255)
                                                                                                                                        aTCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTg      <  1:3164871/149‑1 (MQ=255)
                                                                                                                                        aTCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTATTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGGGCATGTTGTTATCGATTTGCTACGAATGTTGTTCAATGTTg      >  1:3318041/1‑149 (MQ=255)
                                                                                                                                            ttttTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCaaa  <  2:1740507/149‑1 (MQ=255)
                                                                                                                                            ttttTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCaaa  >  1:1543944/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GCGCCAGTCCTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTCATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAA  >  CP009273/647090‑647378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑