Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 662,108 A→T 100% V522V (GTT→GTA)  mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273662,1080AT100.0% 827.0 / ‑6.7 247V522V (GTT→GTA) mrdAtranspeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (129/117);  minor base G (0/1);  total (129/118)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 247 total aligned reads displayed.

GGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCAT  >  CP009273/661963‑662253
                                                                                                                                                 |                                                                                                                                                 
 gTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTacac                                                                                                                                               <  2:1203850/149‑1 (MQ=255)
         aaCGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATa                                                                                                                                       >  1:3499073/1‑149 (MQ=255)
         aaCGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATa                                                                                                                                       >  2:3420189/1‑149 (MQ=255)
              tCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTc                                                                                                                                  <  1:2479936/149‑1 (MQ=255)
              tCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTc                                                                                                                                  >  1:1792096/1‑149 (MQ=255)
              tCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTc                                                                                                                                  >  2:2055085/1‑149 (MQ=255)
                ggCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCtt                                                                                                                                <  1:3485554/149‑1 (MQ=255)
                ggCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCtt                                                                                                                                <  1:1137635/149‑1 (MQ=255)
                ggCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCtt                                                                                                                                <  1:1439165/149‑1 (MQ=255)
                ggCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCtt                                                                                                                                >  1:2826215/1‑149 (MQ=255)
                 gCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCttt                                                                                                                               >  1:3818251/1‑149 (MQ=255)
                   aaTTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCg                                                                                                                             >  1:3700370/1‑149 (MQ=255)
                     ttttGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGcc                                                                                                                           >  1:365614/1‑149 (MQ=255)
                         gtgCGCATGATAGGTTTCGTTCGCGTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCt                                                                                                                       <  1:2361858/149‑1 (MQ=255)
                          tgCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTc                                                                                                                      >  2:1275944/1‑149 (MQ=255)
                           gcgcATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTcc                                                                                                                     >  1:2385346/1‑149 (MQ=255)
                              cATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAg                                                                                                                  >  1:3773899/1‑149 (MQ=255)
                                ttATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTa                                                                                                                >  2:4078476/1‑149 (MQ=255)
                                ttATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTa                                                                                                                >  1:688502/1‑149 (MQ=255)
                                  ataGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAAc                                                                                                              >  2:923001/1‑149 (MQ=255)
                                    aGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCg                                                                                                            <  2:232364/149‑1 (MQ=255)
                                    aGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCg                                                                                                            <  2:2863422/149‑1 (MQ=255)
                                    aGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCg                                                                                                            >  1:3150808/1‑149 (MQ=255)
                                    aGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCg                                                                                                            >  2:3713239/1‑149 (MQ=255)
                                    aGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGCACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCg                                                                                                            >  2:1670396/1‑149 (MQ=255)
                                       tttCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaa                                                                                                         >  2:3818478/1‑149 (MQ=255)
                                       tttCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaa                                                                                                         <  1:3172989/149‑1 (MQ=255)
                                       tttCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaa                                                                                                         >  2:757826/1‑149 (MQ=255)
                                         tcgttcgCTTTCAGACCGAAGACCTGAGCGGTACTGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaatg                                                                                                       <  2:3694736/149‑1 (MQ=255)
                                           gttcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaatgaa                                                                                                     <  1:445628/149‑1 (MQ=255)
                                            ttcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaatgaat                                                                                                    <  1:1566658/149‑1 (MQ=255)
                                             tcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAata                                                                                                   <  2:1229319/149‑1 (MQ=255)
                                              cgCTTTCAGACCGCAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAatat                                                                                                  <  1:1055339/149‑1 (MQ=255)
                                              cgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAatat                                                                                                  <  1:1055019/149‑1 (MQ=255)
                                              cgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAatat                                                                                                  <  1:3626533/149‑1 (MQ=255)
                                              cgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAatat                                                                                                  <  1:1912760/149‑1 (MQ=255)
                                               gCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATc                                                                                                 <  1:1002626/149‑1 (MQ=255)
                                                 tttCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGc                                                                                               <  1:4015229/149‑1 (MQ=255)
                                                   tCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCg                                                                                             >  1:2790508/1‑149 (MQ=255)
                                                    cAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGa                                                                                            <  2:2425935/149‑1 (MQ=255)
                                                     aGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGAc                                                                                           <  1:3791102/149‑1 (MQ=255)
                                                      gACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACg                                                                                          >  1:3976566/1‑149 (MQ=255)
                                                       aCCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACgg                                                                                         >  1:3280397/1‑149 (MQ=255)
                                                       aCCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACgg                                                                                         >  2:1665459/1‑149 (MQ=255)
                                                           aaGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCgg                                                                                     >  2:3516744/1‑149 (MQ=255)
                                                            aGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGt                                                                                    >  2:800955/1‑149 (MQ=255)
                                                             gACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGtt                                                                                   >  2:1348782/1‑149 (MQ=255)
                                                                  gAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGc                                                                              >  2:3246366/1‑149 (MQ=255)
                                                                     cGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGc                                                                           <  1:1519206/149‑1 (MQ=255)
                                                                      ggTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCt                                                                          <  1:1872155/149‑1 (MQ=255)
                                                                       gTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTg                                                                         >  1:2876487/1‑149 (MQ=255)
                                                                       gTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTg                                                                         <  2:313177/149‑1 (MQ=255)
                                                                        tACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGt                                                                        >  1:713332/1‑149 (MQ=255)
                                                                          ccGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTAc                                                                      >  1:186728/1‑149 (MQ=255)
                                                                            ggATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTAccc                                                                    <  2:3663790/149‑1 (MQ=255)
                                                                             gATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCa                                                                   >  2:2978681/1‑149 (MQ=255)
                                                                              aTTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCAt                                                                  >  2:724315/1‑149 (MQ=255)
                                                                               tttCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATg                                                                 <  1:1611119/149‑1 (MQ=255)
                                                                                   gccgcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCAc                                                             >  1:2906101/1‑149 (MQ=255)
                                                                                    ccgcTATGTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCGACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGACTATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCAcc                                                            <  2:2329333/149‑1 (MQ=255)
                                                                                    ccgcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCAcc                                                            <  2:255495/149‑1 (MQ=255)
                                                                                    ccgcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCAcc                                                            <  1:376526/149‑1 (MQ=255)
                                                                                    ccgcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCGTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCAcc                                                            <  2:464706/149‑1 (MQ=255)
                                                                                     cgcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCt                                                           <  2:1513414/149‑1 (MQ=255)
                                                                                        aaTTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGtt                                                        >  2:3536272/1‑149 (MQ=255)
                                                                                         aTTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGttt                                                       >  1:2616821/1‑149 (MQ=255)
                                                                                         aTTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGttt                                                       <  1:741411/149‑1 (MQ=255)
                                                                                           tttGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGc                                                     <  2:927187/149‑1 (MQ=255)
                                                                                            ttGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGcc                                                    <  2:3035896/149‑1 (MQ=255)
                                                                                            ttGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGcc                                                    <  1:2526201/149‑1 (MQ=255)
                                                                                                aCGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCt                                                <  2:2641819/149‑1 (MQ=255)
                                                                                                  ggTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTc                                              <  2:3334517/149‑1 (MQ=255)
                                                                                                   gTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTcg                                             <  2:144234/149‑1 (MQ=255)
                                                                                                     gcgcTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTcggc                                           <  2:2911070/149‑1 (MQ=255)
                                                                                                        cTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGt                                        >  1:1263630/1‑149 (MQ=255)
                                                                                                        cTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGt                                        >  1:1662550/1‑149 (MQ=255)
                                                                                                         tAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTg                                       >  2:2722722/1‑149 (MQ=255)
                                                                                                         tAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTg                                       >  2:24076/1‑149 (MQ=255)
                                                                                                          aGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGc                                      >  2:1625469/1‑149 (MQ=255)
                                                                                                             aaaGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtca                                   <  1:2219038/149‑1 (MQ=255)
                                                                                                               aGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtcatc                                 >  2:3577923/1‑149 (MQ=255)
                                                                                                                  aTTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGc                              <  2:459379/149‑1 (MQ=255)
                                                                                                                          gcCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAgg                      <  2:3565463/149‑1 (MQ=255)
                                                                                                                          gcCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAgg                      <  1:2614693/149‑1 (MQ=255)
                                                                                                                            cGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGaa                    <  2:3006665/149‑1 (MQ=255)
                                                                                                                             gTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAAc                   <  1:1509511/149‑1 (MQ=255)
                                                                                                                             gTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAAc                   >  2:3892022/1‑149 (MQ=255)
                                                                                                                                ccGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCtt                <  2:1753844/149‑1 (MQ=255)
                                                                                                                                ccGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCtt                >  1:1995361/1‑149 (MQ=255)
                                                                                                                                ccGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCtt                <  2:3796029/149‑1 (MQ=255)
                                                                                                                                    tAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAGTATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACg            >  2:1005095/1‑149 (MQ=255)
                                                                                                                                      gggCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGAt          <  2:1964868/149‑1 (MQ=255)
                                                                                                                                      gggCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGAt          >  2:529267/1‑149 (MQ=255)
                                                                                                                                      gggCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGAt          <  2:4006567/149‑1 (MQ=255)
                                                                                                                                      gggCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGAt          <  2:2669196/149‑1 (MQ=255)
                                                                                                                                       ggCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATa         <  2:2792073/149‑1 (MQ=255)
                                                                                                                                           gTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGt     >  1:3993518/1‑149 (MQ=255)
                                                                                                                                              aGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCAt  >  1:1459454/1‑149 (MQ=255)
                                                                                                                                              aGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCAt  >  2:2033252/1‑149 (MQ=255)
                                                                                                                                              aGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCAt  >  1:3349409/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCAT  >  CP009273/661963‑662253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑