Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 667,348 G→T 100% Q99K (CAG→AAG)  lptE ← LPS assembly OM complex LptDE, lipoprotein component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273667,3480GT98.9% 888.9 / 0.1 270Q99K (CAG→AAG) lptELPS assembly OM complex LptDE, lipoprotein component
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (152/115);  total (153/117)
Fisher's exact test for biased strand distribution p-value = 5.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 271 total aligned reads displayed.

TACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACC  >  CP009273/667200‑667493
                                                                                                                                                    |                                                                                                                                                 
tACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTg                                                                                                                                                   >  1:1806251/1‑149 (MQ=255)
  cATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTAt                                                                                                                                                 <  1:2383010/149‑1 (MQ=255)
    tctcTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATAc                                                                                                                                               <  2:3290823/149‑1 (MQ=255)
      tctTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATActc                                                                                                                                             >  1:3715112/1‑149 (MQ=255)
      tctTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATAc                                                                                                                                               >  1:2381030/1‑147 (MQ=255)
        ttttACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTctg                                                                                                                                           <  1:294646/149‑1 (MQ=255)
         tttACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTctgc                                                                                                                                          <  1:2052343/149‑1 (MQ=255)
           tACGATCATGTCTTGTTCGTTATCTTTCTCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTctgctg                                                                                                                                        <  2:1399099/149‑1 (MQ=255)
           tACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTctgctg                                                                                                                                        <  1:3947608/149‑1 (MQ=255)
           tACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTctgctg                                                                                                                                        <  2:1280709/149‑1 (MQ=255)
           tACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTctgctg                                                                                                                                        >  2:3522019/1‑149 (MQ=255)
             cGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGtt                                                                                                                                      >  2:3415954/1‑149 (MQ=255)
              gATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGttt                                                                                                                                     >  1:1070256/1‑149 (MQ=255)
                   tGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCg                                                                                                                                >  1:2104213/1‑149 (MQ=255)
                   tGTCTTGTTAGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCg                                                                                                                                >  1:22318/1‑149 (MQ=255)
                     tCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGtt                                                                                                                              >  1:3946297/1‑149 (MQ=255)
                     tCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGtt                                                                                                                              >  2:2897499/1‑149 (MQ=255)
                         gTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACgg                                                                                                                          >  2:2974117/1‑149 (MQ=255)
                           tCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGaa                                                                                                                        <  1:3035631/149‑1 (MQ=255)
                           tCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGaa                                                                                                                        <  2:1752144/149‑1 (MQ=255)
                             gTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATa                                                                                                                      >  1:3556021/1‑149 (MQ=255)
                              ttATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATAc                                                                                                                     <  2:3533075/149‑1 (MQ=255)
                               tATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATAcc                                                                                                                    >  1:1581068/1‑149 (MQ=255)
                                aTCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGATAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCg                                                                                                                   <  2:3155043/149‑1 (MQ=255)
                                 tCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGa                                                                                                                  >  2:3512784/1‑149 (MQ=255)
                                  cTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGAt                                                                                                                 <  2:2126987/149‑1 (MQ=255)
                                  cTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGAt                                                                                                                 <  2:3799523/149‑1 (MQ=255)
                                   tttCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATg                                                                                                                >  2:9361/1‑149 (MQ=255)
                                    ttCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGc                                                                                                               >  1:2318889/1‑149 (MQ=255)
                                     tCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCg                                                                                                              <  1:2186608/149‑1 (MQ=255)
                                         tAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTAt                                                                                                          >  2:179166/1‑149 (MQ=255)
                                         tAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTAt                                                                                                          <  1:1028372/149‑1 (MQ=255)
                                             gCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCttt                                                                                                      >  1:2124775/1‑149 (MQ=255)
                                              ccATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTc                                                                                                     <  1:1834071/149‑1 (MQ=255)
                                                aTTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTcgc                                                                                                   <  2:2507375/149‑1 (MQ=255)
                                                 tttGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTcgcg                                                                                                  >  2:21899/1‑149 (MQ=255)
                                                 tttGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTcgcg                                                                                                  >  2:19932/1‑149 (MQ=255)
                                                   tGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGAt                                                                                                <  2:442255/149‑1 (MQ=255)
                                                    gCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATg                                                                                               >  1:259755/1‑149 (MQ=255)
                                                     cGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGc                                                                                              <  1:3352875/149‑1 (MQ=255)
                                                       ggTTATCGAAGAACGAACGGAAGACTTTGGCGCTCATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTGTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTc                                                                                            >  1:226361/1‑149 (MQ=255)
                                                            tCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACttt                                                                                       >  2:1826474/1‑149 (MQ=255)
                                                              gaagaaCGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTAc                                                                                     >  2:526757/1‑149 (MQ=255)
                                                              gaagaaCGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTAc                                                                                     >  2:4034123/1‑149 (MQ=255)
                                                              gaagaaCGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTAc                                                                                     >  2:1337386/1‑149 (MQ=255)
                                                              gaagaaCGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTAc                                                                                     <  2:2210288/149‑1 (MQ=255)
                                                               aagaaCGAACGGAAGACTTTGGCGCGAATCGGGGAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGGCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTAcc                                                                                    <  2:4046246/149‑1 (MQ=255)
                                                                   acgaacgGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCaaa                                                                                >  2:2337209/1‑149 (MQ=255)
                                                                   acgaacgGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCaaa                                                                                <  1:3970970/149‑1 (MQ=255)
                                                                     gaacgGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACg                                                                              >  2:633275/1‑149 (MQ=255)
                                                                          gAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAgg                                                                         >  2:2926208/1‑149 (MQ=255)
                                                                            aGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGAt                                                                       <  2:3971791/149‑1 (MQ=255)
                                                                             gACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATg                                                                      <  1:533099/149‑1 (MQ=255)
                                                                              aCTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATgg                                                                     <  1:361503/149‑1 (MQ=255)
                                                                               cTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGa                                                                    <  1:957795/149‑1 (MQ=255)
                                                                               cTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGa                                                                    >  1:1362518/1‑149 (MQ=255)
                                                                                 ttGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAAc                                                                  <  2:1085888/149‑1 (MQ=255)
                                                                                    gcgcTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTTACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTAACCAAACGCAAGGATGGAACGTc                                                               >  2:3103257/1‑149 (MQ=255)
                                                                                     cgcTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGAGGCTCACTTTCCCCAAACGCAAGGATGGAACGTcc                                                              <  1:555628/149‑1 (MQ=255)
                                                                                        tAATCGGGTAGATCTCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTa                                                           <  2:2195482/149‑1 (MQ=255)
                                                                                        tAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTa                                                           >  1:2537718/1‑149 (MQ=255)
                                                                                                tAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGt                                                   >  1:2508881/1‑149 (MQ=255)
                                                                                                tAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGt                                                   <  1:334164/149‑1 (MQ=255)
                                                                                                 aGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGCTGGAACGTCCTTACGCGTGGtt                                                  >  2:3892285/1‑149 (MQ=255)
                                                                                                  gATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGttt                                                 >  2:2955110/1‑149 (MQ=255)
                                                                                                     atCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCtt                                              <  2:2117502/149‑1 (MQ=255)
                                                                                                       cACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTa                                            <  1:3388323/149‑1 (MQ=255)
                                                                                                       cACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTa                                            <  1:3855989/149‑1 (MQ=255)
                                                                                                         cGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATc                                          <  1:4002111/149‑1 (MQ=255)
                                                                                                         cGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATc                                          >  1:2611903/1‑149 (MQ=255)
                                                                                                            ccGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAg                                       <  1:1657693/149‑1 (MQ=255)
                                                                                                              ggggATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCa                                     <  2:1659689/149‑1 (MQ=255)
                                                                                                               gggATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCaa                                    <  1:1021118/149‑1 (MQ=255)
                                                                                                               gggATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCaa                                    >  2:1501914/1‑149 (MQ=255)
                                                                                                                 gATCAACACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCACCt                                  >  1:1209681/1‑149 (MQ=255)
                                                                                                                     aaCACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGac                              >  1:3529839/1‑149 (MQ=255)
                                                                                                                     aaCACGGTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGac                              >  1:2864724/1‑149 (MQ=255)
                                                                                                                          ggTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCAt                         <  2:3947538/149‑1 (MQ=255)
                                                                                                                           gTCGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCAtt                        >  2:1639702/1‑149 (MQ=255)
                                                                                                                             cGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCa                      <  2:2714896/149‑1 (MQ=255)
                                                                                                                             cGCATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCa                      <  1:2711244/149‑1 (MQ=255)
                                                                                                                               cATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGa                    >  1:3458743/1‑149 (MQ=255)
                                                                                                                               cATTAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCAATGTACCCAAACGCAAGGATGTAACGTCCTTACTCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGa                    <  2:2526680/149‑1 (MQ=255)
                                                                                                                                  tAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGt                 >  2:3721048/1‑149 (MQ=255)
                                                                                                                                  tAACCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGt                 <  2:3556021/149‑1 (MQ=255)
                                                                                                                                   aaCCGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTa                >  2:1236943/1‑149 (MQ=255)
                                                                                                                                      cGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACt             <  2:2318889/149‑1 (MQ=255)
                                                                                                                                      cGTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACt             >  2:2634541/1‑149 (MQ=255)
                                                                                                                                       gTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACTACAGTAGGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTg            <  1:4034123/149‑1 (MQ=255)
                                                                                                                                       gTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTg            >  1:1232682/1‑149 (MQ=255)
                                                                                                                                       gTCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTg            >  2:338729/1‑149 (MQ=255)
                                                                                                                                        tCATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTgg           >  1:200816/1‑149 (MQ=255)
                                                                                                                                         cATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGt          <  1:1974126/149‑1 (MQ=255)
                                                                                                                                         cATGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGt          <  1:3251669/149‑1 (MQ=255)
                                                                                                                                          aTGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGtt         >  1:1509385/1‑149 (MQ=255)
                                                                                                                                           tGATCATCTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTa        <  1:72174/149‑1 (MQ=255)
                                                                                                                                                atcTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTAcgcac   >  2:441048/1‑149 (MQ=255)
                                                                                                                                                atcTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTAcgcac   >  1:990494/1‑149 (MQ=255)
                                                                                                                                                atcTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTAcgcac   <  2:1099509/149‑1 (MQ=255)
                                                                                                                                                 tcTTATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTAcgcacc  <  2:2508881/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACC  >  CP009273/667200‑667493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑