Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 672,142 T→A 100% intergenic (‑139/‑25) ybeQ ← / → ybeR Sel1 family TPR‑like repeat protein/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273672,1420TA96.8% 821.0 / 11.3 250intergenic (‑139/‑25)ybeQ/ybeRSel1 family TPR‑like repeat protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (7/1);  new base A (118/124);  total (125/125)
Fisher's exact test for biased strand distribution p-value = 6.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 250 total aligned reads displayed.

AAATCATTATCATTTCCTTATGGTTGCAGATAAAACATATTATATTTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTTAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTATTTAGGTACATGCTTTTTTGACC  >  CP009273/671997‑672287
                                                                                                                                                  |                                                                                                                                                 
aaaTCATTATCATTTCCTTATGGTTGCAGATAAAACATATTATATTTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAAt                                                                                                                                                <  1:2767936/149‑1 (MQ=255)
 aaTCATTATCATTTCCTTATGGTTGCAGATAAAACATATTATATTTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATg                                                                                                                                               >  2:2050035/1‑149 (MQ=255)
   tcattatcattTCCTTATGGTTGCAGATAAAACATATTATATTTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGaa                                                                                                                                             >  2:3289318/1‑149 (MQ=255)
            tttCCTTATGGTTGCAGATAAAACATATTATATTTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAgg                                                                                                                                    >  2:3961236/1‑149 (MQ=255)
            tttCCTTATGGTTGCAGATAAAACATATTATATTTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAgg                                                                                                                                    >  1:1990967/1‑149 (MQ=255)
             ttCCTTATGGTTGCAGATAAAACATATTATATTTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTCTGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGa                                                                                                                                   <  2:1313243/149‑1 (MQ=255)
             ttCCTTATGGTTGCAGATAAAACATATTATATTTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGa                                                                                                                                   <  2:1711223/149‑1 (MQ=255)
               ccTTATGGTTGCAGATAAAACATATTATATTTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTGAAGGATa                                                                                                                                 >  2:2670121/1‑149 (MQ=255)
                 ttATGGTTGCAGATAAAACATATTATATTTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATaaa                                                                                                                               <  2:1577289/149‑1 (MQ=255)
                      gTTGCAGATAAAACATATTATATTTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTTAATGAATATTTAAGGATAAATtata                                                                                                                          >  2:562149/1‑149 (MQ=255)
                      gTTGCAGATAAAACATATTATATTTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATtata                                                                                                                          <  1:1576204/149‑1 (MQ=255)
                      gTTGCAGATAAAACATATTATATTTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATtata                                                                                                                          >  1:3310533/1‑149 (MQ=255)
                           aGATAAAACATATTATATTTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGAc                                                                                                                     >  2:1129130/1‑149 (MQ=255)
                             aTAAAACATATTATATTTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACAt                                                                                                                   >  2:2854597/1‑149 (MQ=255)
                               aaaaCATATTATATTTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATgg                                                                                                                 >  2:726083/1‑149 (MQ=255)
                                 aaCATATTATATTTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAg                                                                                                               >  2:2319328/1‑149 (MQ=255)
                                  aCATATTATATTTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGt                                                                                                              >  1:708643/1‑149 (MQ=255)
                                  aCATATTATATTTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACAAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGt                                                                                                              <  2:1241852/149‑1 (MQ=255)
                                     tattatattTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGc                                                                                                           >  2:1055057/1‑149 (MQ=255)
                                     tattatattTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGc                                                                                                           <  2:1818303/149‑1 (MQ=255)
                                      attatattTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCa                                                                                                          >  1:3905088/1‑149 (MQ=255)
                                      attatattTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCa                                                                                                          >  1:1827403/1‑149 (MQ=255)
                                       ttatattTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCaa                                                                                                         >  2:2576159/1‑149 (MQ=255)
                                         atattTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCaaaa                                                                                                       >  1:1895872/1‑149 (MQ=255)
                                            tttttATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAat                                                                                                    <  2:1941383/149‑1 (MQ=255)
                                            tttttATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAat                                                                                                    >  2:356515/1‑149 (MQ=255)
                                             ttttATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAata                                                                                                   <  1:2664819/149‑1 (MQ=255)
                                              tttATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAatat                                                                                                  <  1:755569/149‑1 (MQ=255)
                                              tttATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAatat                                                                                                  <  2:1187462/149‑1 (MQ=255)
                                              tttATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAatat                                                                                                  >  2:3911431/1‑149 (MQ=255)
                                              tttATGTGTTCAATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAatat                                                                                                  >  2:2105770/1‑149 (MQ=255)
                                              gttATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAatat                                                                                                  >  1:3629239/2‑149 (MQ=255)
                                               ttATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATAtt                                                                                                 >  2:641750/1‑149 (MQ=255)
                                                  tgtgTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGtt                                                                                              <  1:1014169/149‑1 (MQ=255)
                                                    tgtTCGATTTTTGGCGT‑AGCCTTATTTTATGCGTATTATGTCTCTTCAGAAAAGTATAAATAAACGCGCCTAGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTg                                                                                            <  2:3706255/149‑1 (MQ=255)
                                                      ttCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCa                                                                                          >  2:3805930/1‑149 (MQ=255)
                                                         cATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACtt                                                                                       <  2:1108992/149‑1 (MQ=255)
                                                           ttttt‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTc                                                                                     <  2:3096146/149‑1 (MQ=255)
                                                           ttttt‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTc                                                                                     <  2:2764093/149‑1 (MQ=255)
                                                           ttttt‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTc                                                                                     <  2:778820/149‑1 (MQ=255)
                                                             ttt‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCa                                                                                   >  2:368904/1‑149 (MQ=255)
                                                              tt‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCAc                                                                                  <  2:3385944/149‑1 (MQ=255)
                                                                  cGTAAGCCTTATTTTATTCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTcc                                                                               <  2:2538521/149‑1 (MQ=255)
                                                                  cGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTcc                                                                               <  2:2454249/149‑1 (MQ=255)
                                                                  cGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTcc                                                                               <  2:2405680/149‑1 (MQ=255)
                                                                     aaGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGAt                                                                            >  2:2911419/1‑149 (MQ=255)
                                                                      aGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATg                                                                           <  1:438648/149‑1 (MQ=255)
                                                                      aGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATg                                                                           >  2:3395233/1‑149 (MQ=255)
                                                                       gCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATgg                                                                          >  1:3040607/1‑149 (MQ=255)
                                                                       gCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATgg                                                                          >  1:2615518/1‑149 (MQ=255)
                                                                          ttATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGaaa                                                                       <  2:87377/149‑1 (MQ=255)
                                                                          ttATTTGAGGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGaaa                                                                       <  2:3612948/149‑1 (MQ=255)
                                                                           tATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGTAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAAt                                                                      >  2:4092696/1‑149 (MQ=255)
                                                                           tATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAAt                                                                      <  2:3001411/149‑1 (MQ=255)
                                                                            aTTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATa                                                                     <  2:1994622/149‑1 (MQ=255)
                                                                            aTTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATa                                                                     >  2:4106614/1‑149 (MQ=255)
                                                                                   gCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCaatg                                                              <  2:136845/149‑1 (MQ=255)
                                                                                     gTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCaatgaa                                                            >  1:4092897/1‑149 (MQ=255)
                                                                                      tattatGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCaatgaat                                                           <  1:3855254/149‑1 (MQ=255)
                                                                                        ttatGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCaatgaatga                                                         >  2:3436913/1‑148 (MQ=255)
                                                                                           tGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGt                                                      >  1:1100278/1‑149 (MQ=255)
                                                                                             tCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGttt                                                    <  1:1312959/149‑1 (MQ=255)
                                                                                             tCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGttt                                                    >  1:3300914/1‑149 (MQ=255)
                                                                                              cgcgTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTa                                                   >  1:954678/1‑149 (MQ=255)
                                                                                                 gTCAGAAAAGTATAACTAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCt                                                <  2:2098036/149‑1 (MQ=255)
                                                                                                   cAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTc                                              <  2:3482411/149‑1 (MQ=255)
                                                                                                     gAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGc                                            >  2:3301062/1‑149 (MQ=255)
                                                                                                        aaGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCAtt                                         <  1:3703110/149‑1 (MQ=255)
                                                                                                          gTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATtca                                       <  1:2943651/149‑1 (MQ=255)
                                                                                                            ataaataaACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATtcatc                                     <  2:3582842/149‑1 (MQ=255)
                                                                                                            ataaataaACGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATtcatc                                     <  2:830596/149‑1 (MQ=255)
                                                                                                                  aaaCGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCaa                               <  1:2285807/149‑1 (MQ=255)
                                                                                                                   aaCGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCaaa                              >  2:1436718/1‑149 (MQ=255)
                                                                                                                    aCGAGCCTCGCAATGCGGCTAATATTCATTTAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCaaaa                             >  1:1743279/1‑149 (MQ=255)
                                                                                                                    aCGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCaaaa                             <  2:890033/149‑1 (MQ=255)
                                                                                                                     cGAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAAt                            <  2:3814085/149‑1 (MQ=255)
                                                                                                                      gAGCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATg                           <  1:969927/149‑1 (MQ=255)
                                                                                                                        gCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTa                         <  1:1406920/149‑1 (MQ=255)
                                                                                                                        gCCTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTT‑CAAATTACACGGCCATGGTAATAAGCCATTAATGCTGTTTACCGTCGCATTCATCACCCAAAATggtt                        >  1:3444984/1‑146 (MQ=255)
                                                                                                                         ccTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTAt                        <  2:2767715/149‑1 (MQ=255)
                                                                                                                         ccTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTAt                        <  1:2105770/149‑1 (MQ=255)
                                                                                                                         ccTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTAt                        <  2:4012031/149‑1 (MQ=255)
                                                                                                                          cTCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTAtt                       <  1:3975506/149‑1 (MQ=255)
                                                                                                                           tCGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATCTGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTAttt                      <  1:1515888/149‑1 (MQ=255)
                                                                                                                            cGCAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTATTTa                     <  1:746743/149‑1 (MQ=255)
                                                                                                                              cAATGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGACATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTATTTAgg                   >  2:2812317/1‑149 (MQ=255)
                                                                                                                               aaTGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTATTTAGGt                  >  1:703405/1‑149 (MQ=255)
                                                                                                                                aTGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTATTTAGGTa                 >  2:3903988/1‑149 (MQ=255)
                                                                                                                                aTGCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTATTTAGGTa                 >  1:296106/1‑149 (MQ=255)
                                                                                                                                  gCGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTATTTAGGTACa               <  1:2348155/149‑1 (MQ=255)
                                                                                                                                   cGGCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTATTTAGGTACAt              <  1:3473403/149‑1 (MQ=255)
                                                                                                                                    ggCTAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTATTTAGGTACATg             <  2:2262829/149‑1 (MQ=255)
                                                                                                                                       tAATATTCATTTAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTATTTAGGTACATGCtt          >  1:1715155/1‑149 (MQ=255)
                                                                                                                                       tAATATTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTATTTAGGTACATGCtt          >  1:3203312/1‑149 (MQ=255)
                                                                                                                                         atatTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTATTTAGGTACATGCtttt        <  1:196863/149‑1 (MQ=255)
                                                                                                                                          tatTCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTATTTAGGTACATGCttttt       <  2:3366807/149‑1 (MQ=255)
                                                                                                                                          tatCCATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTATTTAGGTACATGCttttt       <  1:2249812/149‑1 (MQ=255)
                                                                                                                                              naTTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTATTTAGGTACATGCTTTTTTGAc   >  1:393594/2‑149 (MQ=255)
                                                                                                                                              cATTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTATTTAGGTACATGCTTTTTTGAc   >  1:2462505/1‑149 (MQ=255)
                                                                                                                                               aTTAAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTATTTAGGTACATGCTTTTTTGAcc  >  2:590804/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AAATCATTATCATTTCCTTATGGTTGCAGATAAAACATATTATATTTTTATGTGTTCCATTTTT‑GCGTAAGCCTTATTTTATGCGTATTATGTCGCGTCAGAAAAGTATAAATAAACGAGCCTCGCAATGCGGCTAATATTCATTTAATGAATATTTAAGGATAAATTATATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAATGAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTATTTAGGTACATGCTTTTTTGACC  >  CP009273/671997‑672287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑