Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 680,846 G→T 100% F180L (TTC→TTA)  gltK ← glutamate, aspartate ABC transporter permease subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273680,8460GT98.0% 831.8 / 5.4 259F180L (TTC→TTA) gltKglutamate, aspartate ABC transporter permease subunit
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base T (146/107);  total (149/110)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 260 total aligned reads displayed.

GGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCA  >  CP009273/680702‑680991
                                                                                                                                                |                                                                                                                                                 
gggTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAAt                                                                                                                                               <  2:1231718/149‑1 (MQ=255)
gggTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAAt                                                                                                                                               >  2:1114494/1‑149 (MQ=255)
gggTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAAt                                                                                                                                               <  2:650061/149‑1 (MQ=255)
    aaTCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGc                                                                                                                                           <  1:2053156/149‑1 (MQ=255)
    aaTCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGc                                                                                                                                           >  2:3984961/1‑149 (MQ=255)
    aaTCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGc                                                                                                                                           <  1:3697836/149‑1 (MQ=255)
     aTCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGcc                                                                                                                                          <  1:4083026/149‑1 (MQ=255)
          tATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGa                                                                                                                                     <  2:1915306/149‑1 (MQ=255)
           aTGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGAt                                                                                                                                    <  1:3641106/149‑1 (MQ=255)
           aTGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGAt                                                                                                                                    >  1:2724183/1‑149 (MQ=255)
            tGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATa                                                                                                                                   <  1:1099238/149‑1 (MQ=255)
            tGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATa                                                                                                                                   >  1:3173366/1‑149 (MQ=255)
             gCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATaa                                                                                                                                  <  2:2810953/149‑1 (MQ=255)
              cTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAac                                                                                                                                 >  1:1519737/1‑149 (MQ=255)
               tGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAaca                                                                                                                                >  1:1079433/1‑149 (MQ=255)
               tGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAaca                                                                                                                                >  2:2033442/1‑149 (MQ=255)
                 tCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCTAGGGATAAcaca                                                                                                                              >  1:3330482/1‑149 (MQ=255)
                 tCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAAcaca                                                                                                                              <  1:3728739/149‑1 (MQ=255)
                 tCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAAcaca                                                                                                                              >  1:329136/1‑149 (MQ=255)
                  ccTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACAt                                                                                                                             >  2:4085836/1‑149 (MQ=255)
                       ttttCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACAcc                                                                                                                        <  1:1355895/149‑1 (MQ=255)
                        tttCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCa                                                                                                                       >  1:3994838/1‑149 (MQ=255)
                           cAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTg                                                                                                                    <  2:3026012/149‑1 (MQ=255)
                            aaGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGa                                                                                                                   >  2:2235353/1‑149 (MQ=255)
                              gTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAgg                                                                                                                 >  1:3920854/1‑149 (MQ=255)
                               tAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGt                                                                                                                >  1:356862/1‑149 (MQ=255)
                                aGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTa                                                                                                               <  2:785963/149‑1 (MQ=255)
                                  cTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATc                                                                                                             <  2:3198216/149‑1 (MQ=255)
                                   tGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATcc                                                                                                            >  2:3591206/1‑149 (MQ=255)
                                    gACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCt                                                                                                           >  1:3441465/1‑149 (MQ=255)
                                            acgacgCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGt                                                                                                   >  1:283474/1‑149 (MQ=255)
                                                   gcTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGc                                                                                            >  2:2607863/1‑149 (MQ=255)
                                                   gcTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGc                                                                                            >  2:2979840/1‑149 (MQ=255)
                                                    cTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGcc                                                                                           >  1:2876064/1‑149 (MQ=255)
                                                     tAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGccc                                                                                          >  1:2561129/1‑149 (MQ=255)
                                                      aaGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCt                                                                                         >  1:539391/1‑149 (MQ=255)
                                                        gACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtga                                                                                       <  2:1480435/149‑1 (MQ=255)
                                                                  gAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAACTCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagcagca                                                                             <  1:3629627/149‑1 (MQ=255)
                                                                   aaaTAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagcagcag                                                                            >  1:387023/1‑149 (MQ=255)
                                                                     aTAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCg                                                                          >  1:2364204/1‑149 (MQ=255)
                                                                     aTAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCg                                                                          >  2:3559439/1‑149 (MQ=255)
                                                                        aaCAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCa                                                                       >  2:1565692/1‑149 (MQ=255)
                                                                           aaaTCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCa                                                                    <  1:2005356/149‑1 (MQ=255)
                                                                            aaTCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCAt                                                                   >  2:249768/1‑149 (MQ=255)
                                                                            aaTCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCAt                                                                   <  2:189183/149‑1 (MQ=255)
                                                                                cGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATcgcg                                                               >  1:2774722/1‑149 (MQ=255)
                                                                                cGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATcgcg                                                               >  1:3390278/1‑149 (MQ=255)
                                                                                cGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATcgcg                                                               >  1:3117796/1‑149 (MQ=255)
                                                                                     aaCAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGaa                                                          <  2:462131/149‑1 (MQ=255)
                                                                                     aaCAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGaa                                                          <  2:462292/149‑1 (MQ=255)
                                                                                        aGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGc                                                       <  2:923176/149‑1 (MQ=255)
                                                                                         ggATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGcc                                                      <  2:3764639/149‑1 (MQ=255)
                                                                                         ggATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGcc                                                      <  2:1919660/149‑1 (MQ=255)
                                                                                          gATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCt                                                     <  1:2240626/149‑1 (MQ=255)
                                                                                          gATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCt                                                     >  1:156229/1‑149 (MQ=255)
                                                                                            tcatcTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGc                                                   >  1:1034859/1‑149 (MQ=255)
                                                                                            tcatcTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGc                                                   >  2:1019345/1‑149 (MQ=255)
                                                                                            tcatcTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGc                                                   >  1:966445/1‑149 (MQ=255)
                                                                                            tcatcTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGc                                                   <  1:573038/149‑1 (MQ=255)
                                                                                             catcTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCg                                                  <  1:1716164/149‑1 (MQ=255)
                                                                                               tctcAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGc                                                >  1:3141311/1‑149 (MQ=255)
                                                                                                   aaCCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaa                                            <  2:1073849/149‑1 (MQ=255)
                                                                                                    aCCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaat                                           <  1:2324196/149‑1 (MQ=255)
                                                                                                     ccTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaata                                          <  1:806271/149‑1 (MQ=255)
                                                                                                     ccTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaata                                          <  1:3150532/149‑1 (MQ=255)
                                                                                                      cTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGactaa                                         >  1:1405054/1‑149 (MQ=255)
                                                                                                      cTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaataa                                         <  1:2961880/149‑1 (MQ=255)
                                                                                                      cTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaataa                                         >  1:3453977/1‑149 (MQ=255)
                                                                                                       tGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaataat                                        >  1:355555/1‑149 (MQ=255)
                                                                                                        gCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATc                                       <  1:102627/149‑1 (MQ=255)
                                                                                                          gTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAg                                     >  1:3240918/1‑149 (MQ=255)
                                                                                                           tACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGt                                    <  1:4000612/149‑1 (MQ=255)
                                                                                                           tACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGt                                    >  2:3617139/1‑149 (MQ=255)
                                                                                                           tACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGt                                    >  2:1570122/1‑149 (MQ=255)
                                                                                                           tACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGt                                    >  2:818666/1‑149 (MQ=255)
                                                                                                               aTCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCa                                >  2:2036002/1‑149 (MQ=255)
                                                                                                                tCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCAt                               >  2:2658660/1‑149 (MQ=255)
                                                                                                                tCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCAt                               >  2:1439526/1‑149 (MQ=255)
                                                                                                                 cACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATc                              <  2:3173291/149‑1 (MQ=255)
                                                                                                                   cGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGa                            <  2:2327993/149‑1 (MQ=255)
                                                                                                                   cGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGa                            >  1:3043571/1‑149 (MQ=255)
                                                                                                                     cTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACt                          >  1:1762376/1‑149 (MQ=255)
                                                                                                                     cTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACt                          <  1:2155402/149‑1 (MQ=255)
                                                                                                                      tCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTg                         >  2:811119/1‑149 (MQ=255)
                                                                                                                      tCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTg                         <  2:2098970/149‑1 (MQ=255)
                                                                                                                      tCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTg                         <  2:3300728/149‑1 (MQ=255)
                                                                                                                       cACCAATGTTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGc                        <  2:2481851/149‑1 (MQ=255)
                                                                                                                       cACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGCTGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGc                        <  2:2408030/149‑1 (MQ=255)
                                                                                                                       cACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGc                        <  1:2190859/149‑1 (MQ=255)
                                                                                                                          cAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAg                     <  1:1872788/149‑1 (MQ=255)
                                                                                                                          cAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAg                     >  1:1328202/1‑149 (MQ=255)
                                                                                                                              ggTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAg                 <  2:1194182/149‑1 (MQ=255)
                                                                                                                                ttGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTc               >  2:3478303/1‑149 (MQ=255)
                                                                                                                                  gAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCAt             >  1:886011/1‑149 (MQ=255)
                                                                                                                                   aGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCAtt            >  2:3358951/1‑149 (MQ=255)
                                                                                                                                     gCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTcc          <  2:356862/149‑1 (MQ=255)
                                                                                                                                      cGGTACGGAATAAATCGGCCAGGGATAACACATACCCCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTccc         <  2:709631/149‑1 (MQ=255)
                                                                                                                                        gTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCaa       <  2:2747712/149‑1 (MQ=255)
                                                                                                                                          aCGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACg     <  2:2941490/149‑1 (MQ=255)
                                                                                                                                             gaataaATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCa  >  2:2280116/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCA  >  CP009273/680702‑680991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑