BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	706,097	A→C	100%	T2T (ACT→ACG) *29G (TGA→GGA)	fur ← uof ←	ferric iron uptake regulon transcriptional repressor; autorepressor ryhB‑regulated fur leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	706,097	0	A	C	98.0%	836.9 / ‑0.4	215	T2T (ACT→ACG) *29G (TGA→GGA)	fur uof	ferric iron uptake regulon transcriptional repressor; autorepressor ryhB‑regulated fur leader peptide
Reads supporting (aligned to +/- strand): ref base A (0/5); new base C (90/120); total (90/125)
Fisher's exact test for biased strand distribution p-value = 7.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 215 total aligned reads displayed.

CAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAAC  >  CP009273/705952‑706242

cAATTTCTTCACCCAGATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAAAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTc                                                                                                                                                <  2:2494419/149‑1 (MQ=255)

 aaTTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCa                                                                                                                                               >  1:1066025/1‑149 (MQ=255)

 aaTTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCa                                                                                                                                               <  2:2383464/149‑1 (MQ=255)

 aaTTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCa                                                                                                                                               <  2:1291332/149‑1 (MQ=255)

 aaTTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCa                                                                                                                                               >  1:3171083/1‑149 (MQ=255)

 aaTTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCa                                                                                                                                               <  1:1534704/149‑1 (MQ=255)

  aTTTCTTCACCCAGATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCAt                                                                                                                                              <  1:3935212/149‑1 (MQ=255)

   tttCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATg                                                                                                                                             >  1:3391737/1‑149 (MQ=255)

   tttCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATg                                                                                                                                             <  1:3172723/149‑1 (MQ=255)

     tcttcACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGGTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCg                                                                                                                                           >  1:815056/1‑149 (MQ=255)

       ttcACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGa                                                                                                                                         >  1:125987/1‑149 (MQ=255)

        tcccccATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGaa                                                                                                                                        <  2:1175061/146‑1 (MQ=255)

          aCCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATc                                                                                                                                      <  2:2070687/149‑1 (MQ=255)

          aCCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATc                                                                                                                                      <  1:336834/149‑1 (MQ=255)

           cccATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCt                                                                                                                                     >  1:1118292/1‑149 (MQ=255)

           cccATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCt                                                                                                                                     <  2:724508/149‑1 (MQ=255)

              atatCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTc                                                                                                                                  >  1:3683729/1‑149 (MQ=255)

               tatCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTcc                                                                                                                                 <  1:3687664/149‑1 (MQ=255)

               tatCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTcc                                                                                                                                 <  2:1315292/149‑1 (MQ=255)

                atCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCt                                                                                                                                >  2:3556099/1‑149 (MQ=255)

                   gATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGtt                                                                                                                             <  1:1481389/149‑1 (MQ=255)

                      cAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACt                                                                                                                          >  2:3787564/1‑149 (MQ=255)

                      cAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACt                                                                                                                          >  1:1875561/1‑149 (MQ=255)

                      cAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACt                                                                                                                          <  2:2265986/149‑1 (MQ=255)

                       aGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTa                                                                                                                         >  1:1664513/1‑149 (MQ=255)

                       aGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTa                                                                                                                         >  2:1351886/1‑149 (MQ=255)

                        gACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTaa                                                                                                                        <  2:3989626/149‑1 (MQ=255)

                         aCGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAg                                                                                                                       <  2:3322225/149‑1 (MQ=255)

                            tttGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCgg                                                                                                                    >  1:1326972/1‑149 (MQ=255)

                              tGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGtt                                                                                                                  <  1:2623288/149‑1 (MQ=255)

                                tataAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCa                                                                                                                <  1:2902381/149‑1 (MQ=255)

                                  taAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACt                                                                                                              >  2:400069/1‑149 (MQ=255)

                                     aTCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCa                                                                                                           >  2:1488735/1‑149 (MQ=255)

                                     aTCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCa                                                                                                           <  1:340095/149‑1 (MQ=255)

                                     aTCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCa                                                                                                           <  2:829946/149‑1 (MQ=255)

                                           cGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTagaa                                                                                                     >  1:27560/1‑149 (MQ=255)

                                                 gACGGGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTg                                                                                               <  2:3150668/149‑1 (MQ=255)

                                                  aCGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGa                                                                                              >  2:2445760/1‑149 (MQ=255)

                                                      gATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCTGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGa                                                                                          <  2:1045918/149‑1 (MQ=255)

                                                      gATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGa                                                                                          >  2:3873833/1‑149 (MQ=255)

                                                       aTGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGaa                                                                                         >  1:3907431/1‑149 (MQ=255)

                                                       aTGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGaa                                                                                         <  1:1994471/149‑1 (MQ=255)

                                                         ggTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAAtt                                                                                       >  1:2296371/1‑149 (MQ=255)

                                                          gTTGTCCGGCTCCTGAAGAACTACCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGAGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAATTTAAAGAAttt                                                                                      >  1:2101517/1‑149 (MQ=255)

                                                            tGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGc                                                                                    <  1:400244/149‑1 (MQ=255)

                                                            tGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGc                                                                                    <  2:417038/149‑1 (MQ=255)

                                                              tCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGctc                                                                                  <  2:3627041/149‑1 (MQ=255)

                                                                        gaagaaCTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAAt                                                                        <  2:1426925/149‑1 (MQ=255)

                                                                         aagaaCTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATg                                                                       >  2:2845829/1‑149 (MQ=255)

                                                                         aagaaCTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATg                                                                       >  1:1717630/1‑149 (MQ=255)

                                                                              cTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTAt                                                                  >  2:2591661/1‑149 (MQ=255)

                                                                                tCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCa                                                                >  2:1976973/1‑149 (MQ=255)

                                                                                tCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCa                                                                <  1:3157291/149‑1 (MQ=255)

                                                                                 ccAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCAt                                                               <  2:233490/149‑1 (MQ=255)

                                                                                  cAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCAtt                                                              <  2:2964288/149‑1 (MQ=255)

                                                                                   aGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATta                                                             <  1:2699430/149‑1 (MQ=255)

                                                                                    ggATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATtat                                                            <  2:3728731/149‑1 (MQ=255)

                                                                                    ggATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATtat                                                            >  2:652494/1‑149 (MQ=255)

                                                                                     gATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATtata                                                           >  2:3091243/1‑149 (MQ=255)

                                                                                      aTTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCAATAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAg                                                          <  1:594370/149‑1 (MQ=255)

                                                                                        ttttAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGaa                                                        <  2:3419467/149‑1 (MQ=255)

                                                                                        ttttAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGaa                                                        >  2:1837677/1‑149 (MQ=255)

                                                                                           tAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTg                                                     <  2:101541/149‑1 (MQ=255)

                                                                                            aagcGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGc                                                    <  2:2732919/146‑1 (MQ=255)

                                                                                            aaaCGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGc                                                    <  2:671405/149‑1 (MQ=255)

                                                                                            aaaCGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGc                                                    <  2:1618136/149‑1 (MQ=255)

                                                                                                  ggAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGcc                                              <  1:2461734/149‑1 (MQ=255)

                                                                                                     aGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTaa                                           >  2:3059909/1‑149 (MQ=255)

                                                                                                      gCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTaaa                                          <  2:3892372/149‑1 (MQ=255)

                                                                                                        gTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATg                                        >  2:1795496/1‑149 (MQ=255)

                                                                                                         ttACGTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGGTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGCATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGa                                       <  2:1970000/149‑1 (MQ=255)

                                                                                                                cAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACcac                                <  1:2981077/149‑1 (MQ=255)

                                                                                                                 aGGCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACcaca                               >  2:2743076/1‑149 (MQ=255)

                                                                                                                  ggCCATCTTTCTTTAGGGGGGTATTGTTATCCTTAATGCGGTATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCTTATCATTATAGAATTGCCACGCCTAAATGAAAACCACaa                              <  1:3502019/149‑1 (MQ=255)

                                                                                                                  ggCCAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACaa                              <  1:686133/149‑1 (MQ=255)

                                                                                                                    ccAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGt                            <  2:208864/149‑1 (MQ=255)

                                                                                                                     cAGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTc                           <  2:2619661/149‑1 (MQ=255)

                                                                                                                      aggtttctttAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTcc                          <  1:2624977/146‑1 (MQ=255)

                                                                                                                      aGCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTcc                          >  2:708406/1‑149 (MQ=255)

                                                                                                                       gCTTTCTTTAGGGCGTTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTccc                         <  1:3037308/149‑1 (MQ=255)

                                                                                                                       gCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCTTTAGAAGAAGTGACATAATTTGCTCTTGAGATATTGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTccc                         <  1:185841/149‑1 (MQ=255)

                                                                                                                       gCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTccc                         <  2:1166902/149‑1 (MQ=255)

                                                                                                                       gCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTccc                         <  2:1163302/149‑1 (MQ=255)

                                                                                                                       gCTTTCTTTAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCACTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTccc                         >  2:4027040/1‑149 (MQ=255)

                                                                                                                        ctttctttAGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCt                        <  1:2391298/149‑1 (MQ=255)

                                                                                                                                aGGGCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAata                <  2:3391737/149‑1 (MQ=255)

                                                                                                                                 gggCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTAGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAatat               >  2:2846119/1‑149 (MQ=255)

                                                                                                                                  ggCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATAtt              <  1:193905/149‑1 (MQ=255)

                                                                                                                                  ggCGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATAtt              >  1:1391979/1‑149 (MQ=255)

                                                                                                                                   gCGGTTTTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTTAGATAATGCGTATCATTATATAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTg             <  2:2421594/149‑1 (MQ=255)

                                                                                                                                    cGGTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTAATTAGAAGAAGTTACAGAATTTGCTCTTGAGATAATGCTTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGt            <  1:918001/149‑1 (MQ=255)

                                                                                                                                     ggTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGtt           >  2:503685/1‑149 (MQ=255)

                                                                                                                                     ggTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGtt           >  2:1565709/1‑149 (MQ=255)

                                                                                                                                      gTATTGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTta          >  1:3964365/1‑149 (MQ=255)

                                                                                                                                         ttGTTATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTtaata       >  1:2954172/1‑149 (MQ=255)

                                                                                                                                             tATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATaaaaa   <  1:3727286/149‑1 (MQ=255)

                                                                                                                                             tATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATaaaaa   >  2:956116/1‑149 (MQ=255)

                                                                                                                                             tATCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATaaaaa   <  2:1664513/149‑1 (MQ=255)

                                                                                                                                              aTCCGTCATGCGTATTCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAAc  <  1:2773885/149‑1 (MQ=255)

                                                                                                                                              aTCCGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAAc  <  1:2220467/149‑1 (MQ=255)

CAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAAC  >  CP009273/705952‑706242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`