Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 76,821 G→T 100% G177G (GGC→GGA)  leuC ← 3‑isopropylmalate dehydratase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927376,8210GT98.4% 799.8 / 3.1 245G177G (GGC→GGA) leuC3‑isopropylmalate dehydratase large subunit
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (134/107);  total (136/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 245 total aligned reads displayed.

ATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGA  >  CP009273/76675‑76967
                                                                                                                                                  |                                                                                                                                                  
 tttCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTccc                                                                                                                                                 <  1:3683804/149‑1 (MQ=255)
  ttCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCg                                                                                                                                                <  1:1891671/149‑1 (MQ=255)
     gATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAATACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGgcg                                                                                                                                             >  2:2852976/1‑149 (MQ=255)
      aTTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGgcgc                                                                                                                                            >  1:38857/1‑149 (MQ=255)
          ccATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCt                                                                                                                                        >  2:3667019/1‑149 (MQ=255)
          ccATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCt                                                                                                                                        <  1:2174235/149‑1 (MQ=255)
          ccATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCt                                                                                                                                        <  1:1731331/149‑1 (MQ=255)
          ccATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCt                                                                                                                                        >  2:1775831/1‑149 (MQ=255)
            atatTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCttt                                                                                                                                      <  1:2314051/149‑1 (MQ=255)
                tGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGccc                                                                                                                                  <  1:1021729/149‑1 (MQ=255)
                tGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGccc                                                                                                                                  <  2:3983842/149‑1 (MQ=255)
                 gCACAGGGTCATACGACCTTCCATGCTTAAATCACGGAGTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCt                                                                                                                                 <  2:3166237/149‑1 (MQ=255)
                        gTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTc                                                                                                                          >  1:1874657/1‑149 (MQ=255)
                           aTACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAAt                                                                                                                       <  1:474857/149‑1 (MQ=255)
                             aCGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAAttt                                                                                                                     <  2:1781436/149‑1 (MQ=255)
                             aCGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAAttt                                                                                                                     >  1:1117404/1‑149 (MQ=255)
                               gACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTc                                                                                                                   >  1:2565928/1‑149 (MQ=255)
                               gACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAAGTTTc                                                                                                                   >  1:2567470/1‑149 (MQ=255)
                                aCCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCa                                                                                                                  >  2:816671/1‑149 (MQ=255)
                                 ccTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCAt                                                                                                                 <  1:1008851/149‑1 (MQ=255)
                                  cTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATg                                                                                                                <  1:2011074/149‑1 (MQ=255)
                                  cTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATg                                                                                                                >  1:81564/1‑149 (MQ=255)
                                   ttCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATgg                                                                                                               >  1:2955069/1‑149 (MQ=255)
                                   ttCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATgg                                                                                                               <  1:2836974/149‑1 (MQ=255)
                                    tCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGt                                                                                                              <  2:202976/149‑1 (MQ=255)
                                     ccATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGtt                                                                                                             <  1:3127732/149‑1 (MQ=255)
                                     ccATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGtt                                                                                                             <  1:1075595/149‑1 (MQ=255)
                                     ccATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGtt                                                                                                             >  2:1001081/1‑149 (MQ=255)
                                       aTGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGtttt                                                                                                           <  1:2473841/149‑1 (MQ=255)
                                        tGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGttttt                                                                                                          <  1:1299712/149‑1 (MQ=255)
                                        tGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGttttt                                                                                                          <  1:1072235/149‑1 (MQ=255)
                                                 cACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGccc                                                                                                 <  1:62222/149‑1 (MQ=255)
                                                        tGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCa                                                                                          <  1:347817/149‑1 (MQ=255)
                                                          cTTCGCCGCAAAACTCCACCACAGGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAgg                                                                                        <  2:1837882/149‑1 (MQ=255)
                                                           ttCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAggg                                                                                       >  1:3013905/1‑149 (MQ=255)
                                                            tCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGt                                                                                      <  1:2215378/149‑1 (MQ=255)
                                                                   aaaaCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGtt                                                                               >  1:929547/1‑149 (MQ=255)
                                                                   aaaaCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTGTCAGGGTTTGCGtt                                                                               >  1:1818313/1‑149 (MQ=255)
                                                                    aaaCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTg                                                                              >  2:3243781/1‑149 (MQ=255)
                                                                    aaaCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTg                                                                              >  2:3449782/1‑149 (MQ=255)
                                                                     aaCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGc                                                                             >  2:1868579/1‑149 (MQ=255)
                                                                      aCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGcc                                                                            >  1:3718603/1‑149 (MQ=255)
                                                                        tCCACCACCTGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAg                                                                          <  2:2475973/149‑1 (MQ=255)
                                                                        tCCACCACCTGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTATTGCGGTAATTCCCGGCGCGGCTTGGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAg                                                                          <  2:2111911/149‑1 (MQ=255)
                                                                          caccacATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTa                                                                        >  1:1383690/1‑149 (MQ=255)
                                                                           accacATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGTGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTAc                                                                       >  2:858078/1‑149 (MQ=255)
                                                                             cacaTGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACgt                                                                     <  1:336833/149‑1 (MQ=255)
                                                                              acaTGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACgtg                                                                    >  2:63921/1‑149 (MQ=255)
                                                                              acaTGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACgtg                                                                    >  1:1265974/1‑149 (MQ=255)
                                                                              acaTGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACgtg                                                                    >  2:1686237/1‑149 (MQ=255)
                                                                              acaTGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACgtg                                                                    <  1:532991/149‑1 (MQ=255)
                                                                               caTGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACgtgt                                                                   >  1:1517438/1‑149 (MQ=255)
                                                                                 tGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTc                                                                 <  2:1931713/149‑1 (MQ=255)
                                                                                  gCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCa                                                                >  2:3599183/1‑149 (MQ=255)
                                                                                      ggTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACtt                                                            <  1:3531160/149‑1 (MQ=255)
                                                                                       gTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTc                                                           >  2:2378466/1‑149 (MQ=255)
                                                                                            gccTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAg                                                      <  1:2407806/149‑1 (MQ=255)
                                                                                             ccTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGTACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGt                                                     >  1:668468/1‑149 (MQ=255)
                                                                                             ccTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGTACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGt                                                     >  1:4037202/1‑149 (MQ=255)
                                                                                             ccTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGt                                                     <  2:2259803/149‑1 (MQ=255)
                                                                                             ccTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGt                                                     <  2:77658/149‑1 (MQ=255)
                                                                                             ccTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGt                                                     <  1:3260699/149‑1 (MQ=255)
                                                                                              cTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTg                                                    <  2:3632755/149‑1 (MQ=255)
                                                                                               tGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGc                                                   <  2:1962623/149‑1 (MQ=255)
                                                                                                  gcgaCCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGGTCAACTTCGGAAGTGCCGa                                                <  2:2526933/146‑1 (MQ=255)
                                                                                                  gcTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGa                                                <  1:3667157/149‑1 (MQ=255)
                                                                                                        ggTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCaa                                          >  1:3282456/1‑149 (MQ=255)
                                                                                                          ttttACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAg                                        >  2:1329986/1‑149 (MQ=255)
                                                                                                          ttttACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAg                                        >  1:1414970/1‑149 (MQ=255)
                                                                                                            ttACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGTGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGc                                      <  1:294178/149‑1 (MQ=255)
                                                                                                            ttACCGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGc                                      <  1:2954592/149‑1 (MQ=255)
                                                                                                               ccGATAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAg                                   >  1:2091654/1‑149 (MQ=255)
                                                                                                                  aTAATTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTgc                                >  2:2217139/1‑149 (MQ=255)
                                                                                                                    aaTTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTgcgc                              >  1:3726075/1‑149 (MQ=255)
                                                                                                                     agggCCAGCACGCTATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGCTACCAAAGGCCAGTGCGcc                             <  2:3689408/146‑1 (MQ=255)
                                                                                                                     aTTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGcc                             >  1:1640322/1‑149 (MQ=255)
                                                                                                                     aTTGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGcc                             <  2:1643647/149‑1 (MQ=255)
                                                                                                                      ttGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCa                            >  1:3985712/1‑149 (MQ=255)
                                                                                                                      ttGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCa                            >  1:2771275/1‑149 (MQ=255)
                                                                                                                       tGCCAGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCaa                           >  1:2194151/1‑149 (MQ=255)
                                                                                                                           aGCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAAcgc                       >  2:1796189/1‑149 (MQ=255)
                                                                                                                            gCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAAcgcg                      >  1:1400815/1‑149 (MQ=255)
                                                                                                                            gCACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAAcgcg                      <  2:3728040/149‑1 (MQ=255)
                                                                                                                             cACGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACgcgc                     <  1:3972860/149‑1 (MQ=255)
                                                                                                                              aCGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGcc                    >  2:1271536/1‑149 (MQ=255)
                                                                                                                              aCGATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGcc                    >  2:1148595/1‑149 (MQ=255)
                                                                                                                                gATATCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCgt                  >  1:2752101/1‑149 (MQ=255)
                                                                                                                                 atatCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCgtg                 <  1:2650773/149‑1 (MQ=255)
                                                                                                                                 atatCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCgtg                 <  1:1367492/149‑1 (MQ=255)
                                                                                                                                 atatCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCgtg                 <  1:160199/149‑1 (MQ=255)
                                                                                                                                 atatCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCgtg                 >  2:2758250/1‑149 (MQ=255)
                                                                                                                                 atatCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGGTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCgtg                 >  2:2757961/1‑149 (MQ=255)
                                                                                                                                  tatCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCgtgg                >  2:2417310/1‑149 (MQ=255)
                                                                                                                                    tCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCgtgggt              >  2:3842918/1‑149 (MQ=255)
                                                                                                                                    tCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCgtgggt              <  1:3842918/149‑1 (MQ=255)
                                                                                                                                    tCTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCgtgggt              >  2:94220/1‑149 (MQ=255)
                                                                                                                                     cTTTTGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCgtgggtg             <  1:2537306/149‑1 (MQ=255)
                                                                                                                                       tttGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGc           <  2:589443/149‑1 (MQ=255)
                                                                                                                                       tttGCGGTAATTCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGc           <  1:851224/149‑1 (MQ=255)
                                                                                                                                                aTGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGa  >  2:476153/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGA  >  CP009273/76675‑76967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑