Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 723,859 G→T 100% V110V (GTC→GTA)  kdpA ← potassium translocating ATPase, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273723,8590GT97.9% 613.3 / 2.1 191V110V (GTC→GTA) kdpApotassium translocating ATPase, subunit A
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (84/103);  total (87/104)
Fisher's exact test for biased strand distribution p-value = 3.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 191 total aligned reads displayed.

CTCTGGCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGAT  >  CP009273/723713‑724004
                                                                                                                                                  |                                                                                                                                                 
    ggCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAg                                                                                                                                             <  1:4077543/149‑1 (MQ=255)
    ggCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAg                                                                                                                                             <  1:1178370/149‑1 (MQ=255)
    ggCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAg                                                                                                                                             <  1:883716/149‑1 (MQ=255)
     gCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGc                                                                                                                                            <  2:1838392/149‑1 (MQ=255)
          tAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGAcg                                                                                                                                       <  2:3811736/149‑1 (MQ=255)
            aaCGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGAcggc                                                                                                                                     >  2:3330908/1‑149 (MQ=255)
              cGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCCGTTGGTATTGGTGACAAAGCTGAcggcgg                                                                                                                                   >  2:860223/1‑149 (MQ=255)
              cGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCCGTTGGTATTGGTGACAAAGCTGAcggcgg                                                                                                                                   >  2:2918465/1‑149 (MQ=255)
                    gATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCCGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCa                                                                                                                             <  1:162787/149‑1 (MQ=255)
                      tGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAgc                                                                                                                           <  2:1553807/149‑1 (MQ=255)
                          ggCAAAAATCACCGCAAGCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCa                                                                                                                       <  2:3546708/149‑1 (MQ=255)
                             aaaaaTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGAt                                                                                                                    <  1:916521/149‑1 (MQ=255)
                                aaTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATccc                                                                                                                 <  2:149868/149‑1 (MQ=255)
                                aaTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATccc                                                                                                                 <  2:3448278/149‑1 (MQ=255)
                                 aTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCa                                                                                                                <  1:471569/149‑1 (MQ=255)
                                 aTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCa                                                                                                                <  2:73618/149‑1 (MQ=255)
                                   cACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACg                                                                                                              >  1:1004565/1‑149 (MQ=255)
                                   cACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACg                                                                                                              >  1:317349/1‑149 (MQ=255)
                                    aCCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGa                                                                                                             >  2:13154/1‑149 (MQ=255)
                                     ccGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGAc                                                                                                            <  1:1676136/149‑1 (MQ=255)
                                      cGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACa                                                                                                           <  2:4000660/149‑1 (MQ=255)
                                      cGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCAGCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACa                                                                                                           >  2:1615372/1‑149 (MQ=255)
                                             ccGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTgg                                                                                                    >  1:3112900/1‑149 (MQ=255)
                                             ccGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCACACGACAGCCCTgg                                                                                                    >  1:706292/1‑149 (MQ=255)
                                               gCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCa                                                                                                  >  1:3289538/1‑149 (MQ=255)
                                                  ggcggcAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAAct                                                                                               >  2:4045092/1‑149 (MQ=255)
                                                     ggcAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAActgct                                                                                            <  2:1039507/149‑1 (MQ=255)
                                                      gcAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAActgctg                                                                                           >  2:43347/1‑149 (MQ=255)
                                                      gcAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAActgctg                                                                                           >  2:39298/1‑149 (MQ=255)
                                                         gaaagaaaGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTgg                                                                                        >  1:3483782/1‑149 (MQ=255)
                                                         gaaagaaaGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTgg                                                                                        <  1:1730247/149‑1 (MQ=255)
                                                           aagaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATATCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAATCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGAt                                                                                      <  2:2678964/149‑1 (MQ=255)
                                                           aagaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGAt                                                                                      >  2:483607/1‑149 (MQ=255)
                                                           aagaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGAt                                                                                      >  2:3028430/1‑149 (MQ=255)
                                                           aagaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGAt                                                                                      >  2:1485585/1‑149 (MQ=255)
                                                            agaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGAtt                                                                                     >  1:3575868/1‑149 (MQ=255)
                                                             gaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTa                                                                                    >  1:1868436/1‑149 (MQ=255)
                                                              aaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTaa                                                                                   <  1:755437/149‑1 (MQ=255)
                                                               aagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAg                                                                                  >  1:1552327/1‑149 (MQ=255)
                                                               aagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAg                                                                                  >  2:1306609/1‑149 (MQ=255)
                                                               aagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAg                                                                                  <  2:768310/149‑1 (MQ=255)
                                                                agTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGc                                                                                 <  2:3460078/149‑1 (MQ=255)
                                                                 gTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCg                                                                                <  1:2688762/149‑1 (MQ=255)
                                                                   tttGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGc                                                                              <  2:2955514/149‑1 (MQ=255)
                                                                       cACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGAt                                                                          <  2:3725068/149‑1 (MQ=255)
                                                                       cACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGAt                                                                          <  2:3869356/149‑1 (MQ=255)
                                                                        aCCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATa                                                                         <  1:3630554/149‑1 (MQ=255)
                                                                          cGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGt                                                                       <  1:3155328/149‑1 (MQ=255)
                                                                            ttAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGc                                                                     >  2:1520043/1‑149 (MQ=255)
                                                                              aaGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTg                                                                   >  2:304415/1‑149 (MQ=255)
                                                                              aaGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTg                                                                   >  1:1208457/1‑149 (MQ=255)
                                                                                gCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGAc                                                                 >  1:1355021/1‑149 (MQ=255)
                                                                                 cccGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGAcc                                                                <  2:3845194/149‑1 (MQ=255)
                                                                                  ccGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCg                                                               >  1:1655093/1‑149 (MQ=255)
                                                                                     ccATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGc                                                            <  2:885807/149‑1 (MQ=255)
                                                                                       aTCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCaa                                                          >  2:1150513/1‑149 (MQ=255)
                                                                                        tCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAAc                                                         >  1:1481587/1‑149 (MQ=255)
                                                                                          tGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACAt                                                       <  1:2024961/149‑1 (MQ=255)
                                                                                          tGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACAt                                                       >  1:3221235/1‑149 (MQ=255)
                                                                                           ggCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATa                                                      <  2:1730482/149‑1 (MQ=255)
                                                                                            gCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATaa                                                     >  2:3948850/1‑149 (MQ=255)
                                                                                             cTGAATTAGCTCATGTTGGTTTCAACGCTATAAGTGTGCATTTGGGTATTGTTTACTTTGGTGACGGCGTTATTCAGCGACAGATCCCACGACAGCCATGGAAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATaaa                                                    <  1:35240/149‑1 (MQ=255)
                                                                                             cTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATaaa                                                    <  1:43347/149‑1 (MQ=255)
                                                                                             cTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATaaa                                                    <  1:39298/149‑1 (MQ=255)
                                                                                                aaaTAGCTCAACGTGGTTTCACCGCTATAAGATTGCCCGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATaaaaaa                                                 >  2:1328295/1‑149 (MQ=255)
                                                                                                 aaTAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAAc                                                <  1:1936093/149‑1 (MQ=255)
                                                                                                  aTAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACa                                               <  2:632752/149‑1 (MQ=255)
                                                                                                  aTAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACa                                               <  2:2176558/149‑1 (MQ=255)
                                                                                                   tAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAg                                              <  2:202812/149‑1 (MQ=255)
                                                                                                      cTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCAc                                           <  1:2076798/149‑1 (MQ=255)
                                                                                                       tCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCAcc                                          <  1:3714242/149‑1 (MQ=255)
                                                                                                         aaCGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGc                                        >  2:2390729/1‑149 (MQ=255)
                                                                                                          aCGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGcc                                       <  1:299198/149‑1 (MQ=255)
                                                                                                             tGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGc                                    <  2:3315683/149‑1 (MQ=255)
                                                                                                               gTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGccc                                  >  1:1292281/1‑149 (MQ=255)
                                                                                                               gTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTCACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTCACCGAGCAACATAAAAAACAGCACCGCCAGccc                                  >  1:2594758/1‑149 (MQ=255)
                                                                                                                tttCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGcccc                                 <  2:1043467/149‑1 (MQ=255)
                                                                                                                tttCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGcccc                                 >  2:2391010/1‑149 (MQ=255)
                                                                                                                 ttCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCa                                >  1:1307532/1‑149 (MQ=255)
                                                                                                                       gCTATAAGATTGCCAGTTGGTATTGTTTACAAAGCTGACGGAGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACAGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGt                          <  2:2059660/149‑1 (MQ=255)
                                                                                                                        cTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGtt                         >  1:1070077/1‑149 (MQ=255)
                                                                                                                        cTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGtt                         >  1:1073902/1‑149 (MQ=255)
                                                                                                                        cTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGtt                         <  2:3576507/149‑1 (MQ=255)
                                                                                                                          ataAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCa                       <  1:3772828/149‑1 (MQ=255)
                                                                                                                          ataAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCa                       <  2:1971739/149‑1 (MQ=255)
                                                                                                                             aGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGc                    <  1:3580140/149‑1 (MQ=255)
                                                                                                                             aGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGc                    >  1:639866/1‑149 (MQ=255)
                                                                                                                              gATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGcc                   >  2:3310179/1‑149 (MQ=255)
                                                                                                                                 tGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGAATGTTCAGGCCgag                <  1:3069592/149‑1 (MQ=255)
                                                                                                                                 tGCCAGGTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCgag                <  2:2559903/149‑1 (MQ=255)
                                                                                                                                   ccAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGaa              <  2:3221248/149‑1 (MQ=255)
                                                                                                                                    cAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAAt             <  1:2919011/149‑1 (MQ=255)
                                                                                                                                      gTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATgg           <  1:2487125/149‑1 (MQ=255)
                                                                                                                                       ttGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGc          >  1:3843766/1‑149 (MQ=255)
                                                                                                                                         ggTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGcac        <  2:1004565/149‑1 (MQ=255)
                                                                                                                                         ggTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGcac        <  2:317349/149‑1 (MQ=255)
                                                                                                                                          gTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGcaca       >  2:3194329/1‑149 (MQ=255)
                                                                                                                                             ttGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAg    >  2:2117526/1‑149 (MQ=255)
                                                                                                                                             ttGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAg    >  2:41725/1‑149 (MQ=255)
                                                                                                                                               ggTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGat  <  1:1034659/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTCTGGCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGAT  >  CP009273/723713‑724004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑