Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 767,399 A→T 100% E162D (GAA→GAT)  cydA → cytochrome d terminal oxidase, subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273767,3990AT98.2% 750.1 / 2.0 227E162D (GAA→GAT) cydAcytochrome d terminal oxidase, subunit I
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base T (115/108);  total (117/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 228 total aligned reads displayed.

TGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGG  >  CP009273/767251‑767542
                                                                                                                                                    |                                                                                                                                               
  ttcttcttcGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATAc                                                                                                                                               >  1:742050/1‑149 (MQ=255)
   tcttcttcGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACt                                                                                                                                              >  1:1043459/1‑149 (MQ=255)
    cttcttcGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTa                                                                                                                                             <  1:705837/149‑1 (MQ=255)
     ttcttcGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTAt                                                                                                                                            <  2:3895396/149‑1 (MQ=255)
      tcttcGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATg                                                                                                                                           <  2:2139652/149‑1 (MQ=255)
         tcGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGt                                                                                                                                        <  2:3693731/149‑1 (MQ=255)
          cGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTa                                                                                                                                       <  2:2975775/149‑1 (MQ=255)
           ggTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTAt                                                                                                                                      >  1:2147021/1‑149 (MQ=255)
            gTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATg                                                                                                                                     >  2:1297115/1‑149 (MQ=255)
                 gATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGAt                                                                                                                                <  2:2692743/149‑1 (MQ=255)
                 gATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGAt                                                                                                                                >  1:2941012/1‑149 (MQ=255)
                  aTCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATg                                                                                                                               <  1:3663653/149‑1 (MQ=255)
                   tCGTCTGGGTAAAGGTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAATCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATgg                                                                                                                              <  2:3535235/149‑1 (MQ=255)
                     gTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTg                                                                                                                            >  1:3457337/1‑149 (MQ=255)
                     gTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTg                                                                                                                            >  1:1510431/1‑149 (MQ=255)
                     gTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTg                                                                                                                            >  1:67387/1‑149 (MQ=255)
                     gTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTg                                                                                                                            <  1:1324586/149‑1 (MQ=255)
                           gTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTtc                                                                                                                      >  2:1410876/1‑149 (MQ=255)
                           gTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTtc                                                                                                                      >  1:3951924/1‑149 (MQ=255)
                           gTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTtc                                                                                                                      <  2:3387796/149‑1 (MQ=255)
                            tAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTtct                                                                                                                     <  1:3105052/149‑1 (MQ=255)
                            tAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTtct                                                                                                                     >  2:3088860/1‑149 (MQ=255)
                             aaaGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTtctc                                                                                                                    <  2:1493530/149‑1 (MQ=255)
                             aaaGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTtctc                                                                                                                    <  2:1340385/149‑1 (MQ=255)
                             aaaGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTtctc                                                                                                                    <  2:1340160/149‑1 (MQ=255)
                             aaaGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTtctc                                                                                                                    <  2:2186016/149‑1 (MQ=255)
                             aaaGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTtctc                                                                                                                    >  1:1158894/1‑149 (MQ=255)
                             aaaGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTtctc                                                                                                                    >  1:1341857/1‑149 (MQ=255)
                             aaaGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTtctc                                                                                                                    <  1:3270215/149‑1 (MQ=255)
                              aaGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTcc                                                                                                                   >  2:2327587/1‑149 (MQ=255)
                               aGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCg                                                                                                                  >  2:767804/1‑149 (MQ=255)
                                gTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGa                                                                                                                 >  2:956823/1‑149 (MQ=255)
                                   cAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCt                                                                                                              <  1:533469/149‑1 (MQ=255)
                                     gCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTgg                                                                                                            <  1:767804/149‑1 (MQ=255)
                                     gCATATGTCTGTGACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCTCTGTGGTTTCTGTTTGCGAACGGCTGGATGCTTAACCCATTAGCGTCAGATTTCAAGTTTGTTACGATGCGTTTGGAGATGTTGAGCGTCTCCGAGTTgg                                                                                                            <  2:1262325/149‑1 (MQ=255)
                                        tatGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGc                                                                                                         >  1:3642745/1‑149 (MQ=255)
                                        tatGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGc                                                                                                         <  1:1499230/149‑1 (MQ=255)
                                         atGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCt                                                                                                        <  1:3694171/149‑1 (MQ=255)
                                         atGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCt                                                                                                        >  2:2416600/1‑149 (MQ=255)
                                          tgtgtgTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCtt                                                                                                       <  2:1037212/149‑1 (MQ=255)
                                           gtgtgtCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTa                                                                                                      <  1:2099629/149‑1 (MQ=255)
                                             gtgtCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAAc                                                                                                    <  2:3248150/149‑1 (MQ=255)
                                              tgtCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAAcc                                                                                                   >  1:304610/1‑149 (MQ=255)
                                              tgtCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAAcc                                                                                                   >  1:4101209/1‑149 (MQ=255)
                                              tgtCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAAcc                                                                                                   >  2:521243/1‑149 (MQ=255)
                                               gtCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAAccc                                                                                                  <  2:2877811/149‑1 (MQ=255)
                                               gtCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAAccc                                                                                                  >  1:2010434/1‑149 (MQ=255)
                                                 cACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCgg                                                                                                >  2:3014055/1‑149 (MQ=255)
                                                   ccTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGtt                                                                                              <  1:2408397/149‑1 (MQ=255)
                                                     tggctggTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGc                                                                                            <  2:604445/149‑1 (MQ=255)
                                                       gctggTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTc                                                                                          <  1:1840426/149‑1 (MQ=255)
                                                          ggtggCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAgg                                                                                       <  1:515564/149‑1 (MQ=255)
                                                             ggCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGa                                                                                    >  2:1822899/1‑149 (MQ=255)
                                                                gcTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAAt                                                                                 >  2:3031004/1‑149 (MQ=255)
                                                                     gTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGtt                                                                            >  1:3348175/1‑149 (MQ=255)
                                                                     gTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGtt                                                                            <  1:2791894/149‑1 (MQ=255)
                                                                       tCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTca                                                                          <  1:3202656/149‑1 (MQ=255)
                                                                        cAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTcac                                                                         <  1:1020637/149‑1 (MQ=255)
                                                                          aaCCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCacac                                                                       >  1:4024765/1‑149 (MQ=255)
                                                                             cTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGt                                                                    >  2:1699433/1‑149 (MQ=255)
                                                                               gTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAg                                                                  >  2:3218772/1‑149 (MQ=255)
                                                                               gTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAg                                                                  >  2:3189303/1‑149 (MQ=255)
                                                                                     aCTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTg                                                            <  1:1964409/149‑1 (MQ=255)
                                                                                         tgGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTa                                                        <  1:711688/149‑1 (MQ=255)
                                                                                             ttCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTAtgtg                                                    <  1:2358076/149‑1 (MQ=255)
                                                                                                 ggTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGGTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTg                                                <  1:562235/149‑1 (MQ=255)
                                                                                                  gTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTgg                                               >  1:1090395/1‑149 (MQ=255)
                                                                                                     gCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGgcgc                                            <  2:3313428/149‑1 (MQ=255)
                                                                                                       gAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGa                                          >  1:2301616/1‑149 (MQ=255)
                                                                                                       gAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGa                                          <  2:1532772/149‑1 (MQ=255)
                                                                                                          cGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGt                                       <  2:2043816/149‑1 (MQ=255)
                                                                                                           ggCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGtt                                      <  2:1167238/149‑1 (MQ=255)
                                                                                                           ggCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGtt                                      <  2:3225832/149‑1 (MQ=255)
                                                                                                             cTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCa                                    >  1:432204/1‑149 (MQ=255)
                                                                                                              tGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCAt                                   >  2:941726/1‑149 (MQ=255)
                                                                                                                gATGCAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATcc                                 >  1:3282014/1‑149 (MQ=255)
                                                                                                                    cAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCgg                             <  2:56884/149‑1 (MQ=255)
                                                                                                                    cAAAACCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCgg                             <  1:630980/149‑1 (MQ=255)
                                                                                                                    aaaaaCCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCgg                             >  2:4042480/2‑149 (MQ=255)
                                                                                                                      aaaCCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTa                           >  2:3648371/1‑149 (MQ=255)
                                                                                                                       aaCCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTAt                          <  1:2335409/149‑1 (MQ=255)
                                                                                                                       aaCCCAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTAt                          >  2:2405690/1‑149 (MQ=255)
                                                                                                                         cccAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCa                        <  2:67375/149‑1 (MQ=255)
                                                                                                                           cAATCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCAgc                      <  2:363331/149‑1 (MQ=255)
                                                                                                                            aaTCGCGTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCAgcg                     >  1:838521/1‑149 (MQ=255)
                                                                                                                                gcgTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCAGCGCATg                 >  2:1835609/1‑149 (MQ=255)
                                                                                                                                gcgTCCGATTTCAACTGTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCAGCGCATg                 <  2:3054750/149‑1 (MQ=255)
                                                                                                                                 cgTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCAGCGCATgg                <  1:3170842/149‑1 (MQ=255)
                                                                                                                                  gTCCGATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCAGCGCATGGt               <  1:1767299/149‑1 (MQ=255)
                                                                                                                                      gATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATTTGACTGGCGCGATGTTCATCCTCGGTATCAGCGCATGGTatat           >  2:2261002/1‑149 (MQ=255)
                                                                                                                                      gATTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCAGCGCATGGTatat           >  2:2367487/1‑149 (MQ=255)
                                                                                                                                       aTTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCAGCGCATGGTATATg          >  1:4006266/1‑149 (MQ=255)
                                                                                                                                       aTTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCAGCGCATGGTATATg          >  1:642396/1‑149 (MQ=255)
                                                                                                                                       aTTTCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCAGCGCATGGTATATg          >  1:2744820/1‑149 (MQ=255)
                                                                                                                                         ttCAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCAGCGCATGGTATATGCt        >  2:3835130/1‑149 (MQ=255)
                                                                                                                                           cAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCAGCGCATGGTATATGCTGa      >  1:158089/1‑149 (MQ=255)
                                                                                                                                           cAACTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCAGCGCATGGTATATGCTGa      >  1:1934091/1‑149 (MQ=255)
                                                                                                                                            aaCTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCAGCGCATGGTATATGCTGaa     >  1:109479/1‑149 (MQ=255)
                                                                                                                                              cTTTGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAg   >  2:2839543/1‑149 (MQ=255)
                                                                                                                                               tttGATACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAgg  >  1:3753579/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TGTTCTTCTTCGGTTGGGATCGTCTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCCGCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGG  >  CP009273/767251‑767542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑