Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 784,094 G→T 100% A67E (GCG→GAG)  galM ← aldose 1‑epimerase; type‑1 mutarotase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273784,0940GT98.5% 869.3 / 2.6 267A67E (GCG→GAG) galMaldose 1‑epimerase; type‑1 mutarotase
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (141/122);  total (143/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 271 total aligned reads displayed.

GCCAGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAGT  >  CP009273/783948‑784239
                                                                                                                                                  |                                                                                                                                                 
gCCAGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAActcg                                                                                                                                                 <  1:700518/149‑1 (MQ=255)
  cAGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGc                                                                                                                                               <  1:2934925/149‑1 (MQ=255)
  cAGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGc                                                                                                                                               <  1:3723321/149‑1 (MQ=255)
      gACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctga                                                                                                                                           <  2:305966/149‑1 (MQ=255)
      gACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctga                                                                                                                                           >  2:2192921/1‑149 (MQ=255)
       aCGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgat                                                                                                                                          <  2:1416583/149‑1 (MQ=255)
         gTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcc                                                                                                                                        <  2:2788122/149‑1 (MQ=255)
         gTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcc                                                                                                                                        <  2:1519674/149‑1 (MQ=255)
         gTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcc                                                                                                                                        <  1:2982683/149‑1 (MQ=255)
         gTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGccggatcc                                                                                                                                        >  2:2828375/1‑149 (MQ=255)
          tttGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcct                                                                                                                                       <  2:1585217/149‑1 (MQ=255)
          tttGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcct                                                                                                                                       <  1:2762309/149‑1 (MQ=255)
          tttGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcct                                                                                                                                       >  2:3330841/1‑149 (MQ=255)
           ttGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcctg                                                                                                                                      >  1:1964691/1‑149 (MQ=255)
           ttGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcctg                                                                                                                                      <  2:1076710/149‑1 (MQ=255)
            tGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcctga                                                                                                                                     <  1:3390550/149‑1 (MQ=255)
            tGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcctga                                                                                                                                     <  1:79845/149‑1 (MQ=255)
               cGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAg                                                                                                                                  >  2:1176344/1‑149 (MQ=255)
                   cccTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCAtt                                                                                                                              <  1:729706/149‑1 (MQ=255)
                   cccTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCAtt                                                                                                                              <  1:3790577/149‑1 (MQ=255)
                   cccTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCAtt                                                                                                                              >  2:3435058/1‑149 (MQ=255)
                     cTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTcc                                                                                                                            <  1:842482/149‑1 (MQ=255)
                     cTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTcc                                                                                                                            <  1:1825151/149‑1 (MQ=255)
                      ttCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCg                                                                                                                           <  2:1103883/149‑1 (MQ=255)
                      ttCCGGCACGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCg                                                                                                                           >  1:1057775/1‑149 (MQ=255)
                          ggCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCt                                                                                                                       <  2:1766559/149‑1 (MQ=255)
                          ggCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCt                                                                                                                       <  2:1766023/149‑1 (MQ=255)
                             ccgccgTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGc                                                                                                                    >  2:3640385/1‑149 (MQ=255)
                             ccgccgTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGc                                                                                                                    >  1:2999562/1‑149 (MQ=255)
                              cgccgTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGca                                                                                                                   <  1:2196452/149‑1 (MQ=255)
                                ccgTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGcaca                                                                                                                 >  1:2779775/1‑149 (MQ=255)
                                    gCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCg                                                                                                             >  2:95507/1‑149 (MQ=255)
                                    gCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCg                                                                                                             >  2:3538911/1‑149 (MQ=255)
                                     cAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGa                                                                                                            <  1:4010471/149‑1 (MQ=255)
                                      aGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGag                                                                                                           >  1:1000977/1‑149 (MQ=255)
                                       gCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGagc                                                                                                          <  1:3335178/149‑1 (MQ=255)
                                       gCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGagc                                                                                                          <  2:374914/149‑1 (MQ=255)
                                         tGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGagcag                                                                                                        >  1:2619138/1‑149 (MQ=255)
                                           gTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAgcg                                                                                                      >  2:2357141/1‑149 (MQ=255)
                                             tAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGcc                                                                                                    <  2:3168440/149‑1 (MQ=255)
                                             tAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGcc                                                                                                    <  1:3613340/149‑1 (MQ=255)
                                             tAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGcc                                                                                                    <  1:3138404/149‑1 (MQ=255)
                                              aaCGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCt                                                                                                   >  1:2330963/1‑149 (MQ=255)
                                               aCGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTc                                                                                                  <  1:225626/149‑1 (MQ=255)
                                                 gCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTcgc                                                                                                <  2:2714772/149‑1 (MQ=255)
                                                 gCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTcgc                                                                                                <  1:1643438/149‑1 (MQ=255)
                                                     tGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGAc                                                                                            <  1:2827262/149‑1 (MQ=255)
                                                           ggCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGcc                                                                                      <  2:2647273/149‑1 (MQ=255)
                                                             cGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCAt                                                                                    <  2:1016714/149‑1 (MQ=255)
                                                             cGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCAt                                                                                    <  1:2679278/149‑1 (MQ=255)
                                                              gAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATc                                                                                   <  2:1870333/149‑1 (MQ=255)
                                                               aaaGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCg                                                                                  >  1:3933647/1‑149 (MQ=255)
                                                               aaaGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCg                                                                                  >  2:624917/1‑149 (MQ=255)
                                                               aaaGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCg                                                                                  >  2:2537992/1‑149 (MQ=255)
                                                               aaaGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCg                                                                                  >  1:4055558/1‑149 (MQ=255)
                                                                aaGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCgg                                                                                 >  2:799775/1‑149 (MQ=255)
                                                                  gCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGaa                                                                               <  2:3451783/149‑1 (MQ=255)
                                                                    gTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAg                                                                             >  1:2690438/1‑149 (MQ=255)
                                                                     tCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGc                                                                            <  2:151023/149‑1 (MQ=255)
                                                                     tCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCGGCCCTCGGAAAGc                                                                            >  1:115579/1‑149 (MQ=255)
                                                                       aCGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCgg                                                                          <  2:2014580/149‑1 (MQ=255)
                                                                        cGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGa                                                                         >  1:2885664/1‑149 (MQ=255)
                                                                         ggTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGaa                                                                        <  1:2108393/149‑1 (MQ=255)
                                                                           tttCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATa                                                                      <  2:1109581/149‑1 (MQ=255)
                                                                            ttCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATAc                                                                     <  2:3393502/149‑1 (MQ=255)
                                                                               aCCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACggg                                                                  >  1:1245477/1‑149 (MQ=255)
                                                                                      aaGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGACTACGGGCGGaaag                                                           >  2:2261884/1‑149 (MQ=255)
                                                                                           ataACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagtaaag                                                      >  2:3102694/1‑149 (MQ=255)
                                                                                           ataACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagtaaag                                                      >  1:3527415/1‑149 (MQ=255)
                                                                                           ataACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATAGGAAAGCGGAATACGGTCGGaaagtaaag                                                      >  2:2576565/1‑149 (MQ=255)
                                                                                              aCGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCg                                                   >  1:962678/1‑149 (MQ=255)
                                                                                               cGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGc                                                  <  1:2680007/149‑1 (MQ=255)
                                                                                               cGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGc                                                  <  2:3232416/149‑1 (MQ=255)
                                                                                                ggCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCa                                                 <  2:1976052/149‑1 (MQ=255)
                                                                                                   tATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCAccc                                              <  2:833876/149‑1 (MQ=255)
                                                                                                    aTTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCAcccc                                             >  1:3094976/1‑149 (MQ=255)
                                                                                                    aTTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCAcccc                                             >  2:617613/1‑149 (MQ=255)
                                                                                                      tGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAg                                           >  2:2844888/1‑149 (MQ=255)
                                                                                                       ggCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGt                                          <  1:1270961/149‑1 (MQ=255)
                                                                                                       ggCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGt                                          >  2:3279646/1‑149 (MQ=255)
                                                                                                             aCGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCa                                    <  1:2968833/149‑1 (MQ=255)
                                                                                                             aCGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCa                                    <  1:3200905/149‑1 (MQ=255)
                                                                                                                gTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCg                                 >  2:4093259/1‑149 (MQ=255)
                                                                                                                  tGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTg                               <  1:2501567/149‑1 (MQ=255)
                                                                                                                    gCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGAc                             >  1:63640/1‑149 (MQ=255)
                                                                                                                    gCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGAc                             >  2:2816882/1‑149 (MQ=255)
                                                                                                                    gCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGAc                             >  2:755535/1‑149 (MQ=255)
                                                                                                                       tAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTAc                          >  1:443265/1‑149 (MQ=255)
                                                                                                                          cGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCAt                       <  1:343373/149‑1 (MQ=255)
                                                                                                                            aCCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGTAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATcc                     >  1:1448153/1‑149 (MQ=255)
                                                                                                                             ccAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATccc                    <  1:1207148/149‑1 (MQ=255)
                                                                                                                             ccAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATccc                    <  1:2092919/149‑1 (MQ=255)
                                                                                                                                 tAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCg                >  1:580052/1‑149 (MQ=255)
                                                                                                                                  agagGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCgt               <  2:628020/149‑1 (MQ=255)
                                                                                                                                       cccccAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTa          >  2:2924002/1‑149 (MQ=255)
                                                                                                                                        ccccAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTAc         <  1:1910014/149‑1 (MQ=255)
                                                                                                                                        ccccAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTAc         <  1:1116015/149‑1 (MQ=255)
                                                                                                                                               aaCTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAGt  <  2:3883965/149‑1 (MQ=255)
                                                                                                                                               aaCTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAGt  >  1:4087667/1‑149 (MQ=255)
                                                                                                                                               aaCTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAGt  >  1:2483899/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCCAGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAGT  >  CP009273/783948‑784239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑