Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 805,770 A→T 6.2% Q324L (CAA→CTA)  bioB → biotin synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273805,7700AT 6.2% 667.6 / 25.9 211Q324L (CAA→CTA) bioBbiotin synthase
Reads supporting (aligned to +/- strand):  ref base A (112/86);  new base T (5/8);  total (117/94)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 224 total aligned reads displayed.

TGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGC  >  CP009273/805623‑805918
                                                                                                                                                   |                                                                                                                                                    
tgtgCTATATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGaacaa                                                                                                                                                     <  1:887942/149‑1 (MQ=255)
 gtgCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGaacaac                                                                                                                                                    <  1:1441866/149‑1 (MQ=255)
      ttATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATCACGAACAACAGCaa                                                                                                                                               >  2:2133976/1‑149 (MQ=255)
      ttATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCaa                                                                                                                                               <  1:445815/149‑1 (MQ=255)
      ttATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCAGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCaa                                                                                                                                               <  2:3732944/149‑1 (MQ=255)
       tATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAAc                                                                                                                                              >  2:162401/1‑149 (MQ=255)
       tATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAAc                                                                                                                                              >  2:2236546/1‑149 (MQ=255)
          ggCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTc                                                                                                                                           <  2:2827680/149‑1 (MQ=255)
            cAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCtt                                                                                                                                         <  1:3244690/149‑1 (MQ=255)
              ggCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGa                                                                                                                                       <  1:352005/149‑1 (MQ=255)
               gcgcAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGaa                                                                                                                                      <  2:1985560/149‑1 (MQ=255)
                 gcAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACa                                                                                                                                    <  2:3051952/149‑1 (MQ=255)
                    aaCTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGgc                                                                                                                                 >  1:3310570/1‑149 (MQ=255)
                      cTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGgcgc                                                                                                                               <  2:2755704/149‑1 (MQ=255)
                       tCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCt                                                                                                                              >  2:1498948/1‑149 (MQ=255)
                         gATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtga                                                                                                                            <  1:3124561/149‑1 (MQ=255)
                         gATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtga                                                                                                                            >  1:2047731/1‑149 (MQ=255)
                          aTTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtgat                                                                                                                           >  2:2385372/1‑149 (MQ=255)
                           ttttCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACTACAGCAACGTCTTGAACAGGCGCtgatg                                                                                                                          <  2:784408/149‑1 (MQ=255)
                           ttttCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtgatg                                                                                                                          <  2:912156/149‑1 (MQ=255)
                              tCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGAcc                                                                                                                       >  1:167913/1‑149 (MQ=255)
                               cTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGAccc                                                                                                                      <  2:2663953/149‑1 (MQ=255)
                               cTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGAccc                                                                                                                      <  2:430226/149‑1 (MQ=255)
                                tACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGAcccc                                                                                                                     <  1:3612541/149‑1 (MQ=255)
                                tACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGAcccc                                                                                                                     <  1:1566149/149‑1 (MQ=255)
                                     ttGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGaca                                                                                                                >  2:1420126/1‑149 (MQ=255)
                                         aaaCTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACcga                                                                                                            >  1:2698359/1‑149 (MQ=255)
                                         aaaCTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACcga                                                                                                            <  2:47745/149‑1 (MQ=255)
                                         aaaCTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACcga                                                                                                            <  1:12174/149‑1 (MQ=255)
                                          aaCTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACcgac                                                                                                           <  2:3941248/149‑1 (MQ=255)
                                             tgctgACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGaa                                                                                                        <  2:3333701/149‑1 (MQ=255)
                                              gctgACCACTCCGACTCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATACGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAat                                                                                                       >  2:2792680/1‑149 (MQ=255)
                                               ctgACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAata                                                                                                      <  1:2237113/149‑1 (MQ=255)
                                               ctgACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAata                                                                                                      <  1:687244/149‑1 (MQ=255)
                                                tgACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGGTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAatat                                                                                                     <  1:2563507/149‑1 (MQ=255)
                                                tgACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAatat                                                                                                     >  1:4097922/1‑149 (MQ=255)
                                                 gACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAAGAtt                                                                                                    >  2:3231217/1‑149 (MQ=255)
                                                 gACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCATCCTCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATAtt                                                                                                    >  1:4034154/1‑149 (MQ=255)
                                                     aCGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACTACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACaa                                                                                                <  2:675938/149‑1 (MQ=255)
                                                     aCGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACTACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACaa                                                                                                <  2:675982/149‑1 (MQ=255)
                                                     aCGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCAGAAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATAACaa                                                                                                >  1:3493591/1‑149 (MQ=255)
                                                      ctccGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAAc                                                                                               >  1:1558983/3‑149 (MQ=255)
                                                       tccGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAAcg                                                                                              >  1:242441/2‑149 (MQ=255)
                                                         cGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACTACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAAcgcg                                                                                            <  1:3861688/149‑1 (MQ=255)
                                                          gAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCgg                                                                                           >  2:572802/1‑149 (MQ=255)
                                                           aaTCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGgc                                                                                          >  1:1769375/1‑149 (MQ=255)
                                                            aTCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGgca                                                                                         >  2:1458014/1‑149 (MQ=255)
                                                            aTCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGgca                                                                                         >  2:2163379/1‑149 (MQ=255)
                                                            aTCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGgca                                                                                         >  2:264239/1‑149 (MQ=255)
                                                            aTCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGgca                                                                                         >  1:452403/1‑149 (MQ=255)
                                                                ggAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCAt                                                                                     >  2:3803270/1‑149 (MQ=255)
                                                                     aagaTAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGa                                                                                <  1:2841330/149‑1 (MQ=255)
                                                                        aTAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCt                                                                             >  2:471072/1‑149 (MQ=255)
                                                                        aTAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCt                                                                             >  2:1791116/1‑149 (MQ=255)
                                                                         tAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGACTATTACAACGCGGCAGCAGTATGAGCtt                                                                            >  2:3084663/1‑148 (MQ=255)
                                                                         tAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTg                                                                            >  2:1925496/1‑149 (MQ=255)
                                                                            aGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCa                                                                         >  2:1081576/1‑149 (MQ=255)
                                                                            aGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCa                                                                         <  1:1075399/149‑1 (MQ=255)
                                                                                cTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGgag                                                                     >  1:1279065/1‑149 (MQ=255)
                                                                                 tGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACTACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGgaga                                                                    >  2:1330522/1‑149 (MQ=255)
                                                                                 tGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACTACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGgaga                                                                    >  2:901331/1‑149 (MQ=255)
                                                                                 tGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGgaga                                                                    >  1:3685412/1‑149 (MQ=255)
                                                                                    aaCTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGaaaa                                                                 <  1:1929856/149‑1 (MQ=255)
                                                                                     aCTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAAt                                                                >  1:1042526/1‑149 (MQ=255)
                                                                                     aCTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAAt                                                                >  1:1050229/1‑149 (MQ=255)
                                                                                        gTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACTACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCaa                                                             <  1:2318048/149‑1 (MQ=255)
                                                                                        gTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCaa                                                             >  1:63214/1‑149 (MQ=255)
                                                                                         ttCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAAc                                                            >  1:1160001/1‑149 (MQ=255)
                                                                                           ccGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAAcgc                                                          <  2:2648006/149‑1 (MQ=255)
                                                                                           ccGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAAcgc                                                          <  1:838827/149‑1 (MQ=255)
                                                                                           ccGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAAcgc                                                          >  1:1695803/1‑149 (MQ=255)
                                                                                           ccGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAAcgc                                                          >  1:2782679/1‑149 (MQ=255)
                                                                                             gCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACgcgg                                                        >  2:1137289/1‑149 (MQ=255)
                                                                                             gCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACgcgg                                                        >  1:785176/1‑149 (MQ=255)
                                                                                                aaCTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGgcgc                                                     >  2:288132/1‑149 (MQ=255)
                                                                                                 aCTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCt                                                    >  2:2466901/1‑149 (MQ=255)
                                                                                                  cTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTc                                                   <  1:1830454/149‑1 (MQ=255)
                                                                                                   tGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCg                                                  >  2:2947635/1‑149 (MQ=255)
                                                                                                    ggggCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGa                                                 >  1:308821/1‑149 (MQ=255)
                                                                                                    ggggCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGa                                                 >  1:48959/1‑149 (MQ=255)
                                                                                                     gggCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGAt                                                >  1:3213978/1‑149 (MQ=255)
                                                                                                      ggCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATg                                               >  1:1210745/1‑149 (MQ=255)
                                                                                                         tAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATgcgc                                            >  1:2021227/1‑149 (MQ=255)
                                                                                                          aaaTCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGcgcg                                           <  2:4031208/149‑1 (MQ=255)
                                                                                                           aaTCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACTACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCgcgg                                          >  2:970697/1‑149 (MQ=255)
                                                                                                             tCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCgcggcg                                        >  2:3611664/1‑149 (MQ=255)
                                                                                                                gcagcaAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGtgc                                     <  1:4054591/149‑1 (MQ=255)
                                                                                                                 cagcaAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGtgct                                    <  1:1130849/149‑1 (MQ=255)
                                                                                                                      aaCTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGa                               <  1:3262900/149‑1 (MQ=255)
                                                                                                                      aaCTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGa                               <  2:2529346/149‑1 (MQ=255)
                                                                                                                        cTGCCGTGCTGGCCGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATg                             >  2:528434/1‑149 (MQ=255)
                                                                                                                            cGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCt                         <  2:2099781/149‑1 (MQ=255)
                                                                                                                              tGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGc                       >  1:574161/1‑149 (MQ=255)
                                                                                                                                  ggCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCg                   >  2:1808268/1‑149 (MQ=255)
                                                                                                                                      gggtATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGt               >  1:1606838/1‑149 (MQ=255)
                                                                                                                                      ggggATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGt               <  1:394567/149‑1 (MQ=255)
                                                                                                                                           tAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATcc          >  1:2565115/1‑149 (MQ=255)
                                                                                                                                                aacaacAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTgg     >  2:2152003/1‑149 (MQ=255)
                                                                                                                                                 acaacaGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGgc    >  1:2855545/1‑149 (MQ=255)
                                                                                                                                                 acaacaGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGgc    >  2:2637818/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGC  >  CP009273/805623‑805918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑