Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 816,078 C→G 8.8% intergenic (+34/‑171) ybhL → / → ybhM UPF0005 family inner membrane protein/UPF0005 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273816,0780CG 8.8% 626.4 / 7.2 220intergenic (+34/‑171)ybhL/ybhMUPF0005 family inner membrane protein/UPF0005 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (91/106);  new base G (5/18);  total (96/124)
Fisher's exact test for biased strand distribution p-value = 2.73e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

ACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATT  >  CP009273/815931‑816226
                                                                                                                                                   |                                                                                                                                                    
  ccGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCggg                                                                                                                                                   >  2:3330404/1‑149 (MQ=255)
    gcgACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGAt                                                                                                                                                 <  1:204058/149‑1 (MQ=255)
    gcgACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATACCGGCCATGTGTggggct                                                                                                                                                 >  1:3541242/1‑147 (MQ=255)
        cacGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCTTCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGAttttt                                                                                                                                             >  2:1007020/1‑149 (MQ=255)
          cGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGAttttttt                                                                                                                                           <  2:3769049/149‑1 (MQ=255)
           gTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTc                                                                                                                                          >  1:2648745/1‑149 (MQ=255)
           gTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTAACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTc                                                                                                                                          >  1:1115587/1‑149 (MQ=255)
             cGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCtt                                                                                                                                        <  1:3625781/149‑1 (MQ=255)
               aaCCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTAc                                                                                                                                      <  2:1290908/149‑1 (MQ=255)
                   tGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGCTTTTTTTGTTACTaaa                                                                                                                                  >  1:3956063/1‑149 (MQ=255)
                   tGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTaaa                                                                                                                                  >  1:2374468/1‑149 (MQ=255)
                   tGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTATTTTTCACCATCACTATACCGGCCATGTGTCGGGATTTTTTTCTTACTaaa                                                                                                                                  >  1:662915/1‑149 (MQ=255)
                     cgcAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCAGGTGTCGGGATTTTTTTCTTACTaaaaa                                                                                                                                <  2:2850421/149‑1 (MQ=255)
                       cAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTGAATTCTTTTTAACCATAACTATACCGGCCATGGGTGGGGGTTTTTTTATTTATaaaaaag                                                                                                                              >  1:1044700/1‑147 (MQ=255)
                        aaaTATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGa                                                                                                                             <  2:1686259/149‑1 (MQ=255)
                        aaaTATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGa                                                                                                                             <  1:3226481/149‑1 (MQ=255)
                         aaTTTTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGTATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGac                                                                                                                            <  2:459514/149‑1 (MQ=255)
                         aaTATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGac                                                                                                                            >  1:2382427/1‑149 (MQ=255)
                          atatTCCTTTCTTGGCGCGTTAACCTTTTATCTGGACTAAAACAACATGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAACACGaca                                                                                                                           <  2:1936892/149‑1 (MQ=255)
                            atTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACt                                                                                                                         >  2:3598287/1‑149 (MQ=255)
                            atTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACt                                                                                                                         >  2:3183635/1‑149 (MQ=255)
                                cATTCTTGGCGCTTTAACCGTTTATCTGGAGTTCATCAACGTGTTCCTGATGTTGTTGGGGATCTTCGGCAACCGCGTTTAATTCTTTTTCACCATCACTATCCCGGCATTGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAAc                                                                                                                     <  2:3463976/149‑1 (MQ=255)
                                  ttCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACaa                                                                                                                   >  2:2954264/1‑149 (MQ=255)
                                             ttAACGTTTTATCTGGACTACATAAACTTGTTCCTTTTGTTGTTGCGTATCTTCGGCAACCGCCTTTATTTATTTTACACCATCAATAGCCCGGCCTTGTGTGGTTTTTTTTTTCTTAATAAAAACGACACTTAACAAGTACCAGGtat                                                                                                        <  1:1447336/149‑1 (MQ=255)
                                             ttAACGTTGTATCTGGACTTCATCACCCTGTTCCTGATGTTGTTGGGTATCTTCGGCAACCGCGGTTAATTTTTTTTCACCATCACTATCCCGGCAATGTGTGGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGtat                                                                                                        <  1:3585730/149‑1 (MQ=255)
                                             ttAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGtat                                                                                                        >  2:1139948/1‑149 (MQ=255)
                                                 ccTGGTATCTGCACTTCAACAACCTGTTCCTGATTTTGTTGCGTATCTTCGGCAACCGCCTTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATaaaa                                                                                                    <  2:3276171/149‑1 (MQ=255)
                                                   ttGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTGAATTATTTTTCACCATCACAATCCCGGCCAAGTGTGGGGATTTTTTTCTTAATAAAAAAGAAACATAACAAATAACACGTATaaaaaa                                                                                                  >  1:3798413/1‑147 (MQ=255)
                                                       aTCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCTTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGcc                                                                                              <  2:857549/149‑1 (MQ=255)
                                                       aTCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGcc                                                                                              <  2:2137496/149‑1 (MQ=255)
                                                          tGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTa                                                                                           <  2:1932064/149‑1 (MQ=255)
                                                            gACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCAACAACACAAAACCGGCCCTGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAAAAAGTACCACGTATAAAATATGCCTTATg                                                                                         >  1:3138885/1‑149 (MQ=255)
                                                             aCTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATgg                                                                                        <  2:2467350/149‑1 (MQ=255)
                                                              cTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGa                                                                                       >  2:417547/1‑149 (MQ=255)
                                                              cTTCATAAACCTGTTCCTGTTTTTTTTGGGGATCTTCGGAAACCGCCTTTAATTCTTTTTCACCATCACTATCCCGCCCATGTGTGGGTATTTTTTTCTTACTAAACACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGa                                                                                       <  1:2526959/149‑1 (MQ=255)
                                                                 catcaACCTGTTCGTGTTGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTACACCATCACTATCCCGGCATTGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGa                                                                                    <  1:3340450/149‑1 (MQ=255)
                                                                   tcaACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGc                                                                                  <  2:686549/149‑1 (MQ=255)
                                                                       cctgttcctgttGTTGTTGCGTATCTTCGGCAACCGCCTTTAATTATTTTACACCATCACTAGCCCGGCAATGTGTGGGTTTTTTTTTCTCACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGaa                                                                              <  1:1187621/149‑1 (MQ=255)
                                                                       ccTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCCTGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGaa                                                                              <  2:2882300/149‑1 (MQ=255)
                                                                         tGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTATTTTTCACCAACAAAATACCCGCCATGAGTCGGGATTTTTTTCTTAATAAAAAAGACACATAACAATTAACACGTCTAAAAAAATCCTTAATGACGAGCCGaacc                                                                            >  1:1972391/1‑147 (MQ=255)
                                                                          gTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCg                                                                           >  2:3835209/1‑149 (MQ=255)
                                                                           ttCGTGATGGTGTGGCGTATCTTCGCCAACCGCTTTTAATACTTTTACACAAACACTATCCCGCCCATGTGTGGTTTTTTTTTTATTACAAAACACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGt                                                                          <  1:1400165/149‑1 (MQ=255)
                                                                           ttCCTTCTGTTGTTGCGGATGTTCGGCAACCGCCGTTATTTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGt                                                                          <  2:2899298/149‑1 (MQ=255)
                                                                               tGATGTTGTTGCGGATCTTCGGCAACCGCCTTTAATTCTTTTTCACCATCACTATCCCGGCCTTGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTc                                                                      <  1:224314/149‑1 (MQ=255)
                                                                               tGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCTTGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTc                                                                      <  2:767483/149‑1 (MQ=255)
                                                                               tGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTc                                                                      >  2:511393/1‑149 (MQ=255)
                                                                                gATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCt                                                                     >  1:3771245/1‑149 (MQ=255)
                                                                                 aTGTTGTTGCGTATCTTCGGCAACCGCCTTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTg                                                                    <  2:662915/149‑1 (MQ=255)
                                                                                  tgttgtTGGGGATCTTCGGCAACCGCGGTTAATTCTTTTTCACCATCACTAGCCCGGCCTTGGGTCGGTTTTTTTTTGTTAATAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGt                                                                   <  1:4020769/149‑1 (MQ=255)
                                                                                   gttgttGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTa                                                                  <  2:2577130/149‑1 (MQ=255)
                                                                                       ttgCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCAACACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAACACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCa                                                              <  2:51309/149‑1 (MQ=255)
                                                                                           gtaTCTTCGGCAACCGCCGTTATTTCTTTTTCACCATCACTACCCCGGCCATGTGTGGGTTTTTTTTTCTTACTAAACACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATaaa                                                          <  1:785014/147‑1 (MQ=255)
                                                                                           ggATCTTCGGCAACCGCCTTTAATTCTTTTACACCATCACTATCCCGGCCTTGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATaaa                                                          <  1:1670951/149‑1 (MQ=255)
                                                                                              tCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATACATAAATGc                                                       >  1:4095575/1‑149 (MQ=255)
                                                                                              tCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACAATCCCGGCCAAGTGTCGGGATTTTTTTGTTACTAAAAAAGACACATAACAATTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTGTGTATACATAAATGc                                                       >  1:3008385/1‑149 (MQ=255)
                                                                                               cTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTGGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCa                                                      <  1:3436879/149‑1 (MQ=255)
                                                                                                 tCGGAAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCTTGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGAGTTTTCTGTATCCATAAATGCaaa                                                    <  1:3428988/149‑1 (MQ=255)
                                                                                                   ggCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTGGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAg                                                  <  1:3459371/149‑1 (MQ=255)
                                                                                                   ggCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTGGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATTCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAg                                                  >  2:1582915/1‑149 (MQ=255)
                                                                                                    gCAACCGCCGTTAATTCTTTTTAACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTAATAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATTGACGAGCGGAAGCGTTTTCTGTATCCATAAATTCAAAAGt                                                 >  2:1396554/1‑149 (MQ=255)
                                                                                                       aCCGCAGTTAATTCTTTTTCACCATCACTATCCCGGCCTTGTGTGGGTTTTTTTTTCTTACAAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTAtt                                              <  1:2794747/149‑1 (MQ=255)
                                                                                                        ccgccgTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTAttt                                             <  2:2640321/149‑1 (MQ=255)
                                                                                                        ccgccgTTAAATATTTTTAACCAACACAATACCCGCCATGTGTCGGGATTTTTTTATTACTAAAAACGACACATAACAAGTACCAGGTATAAAATATTCCTTATTGACCAGCGGAAGCGTTTTCTGTATCCATAAAAGCAAAAGTAttt                                             >  2:2544760/1‑149 (MQ=255)
                                                                                                         cgccgTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATTCCTTATGGACGAGCGGAAGCGTTTTGTGTATCCATAAATTCAAAAGTAtttt                                            >  1:2474899/1‑149 (MQ=255)
                                                                                                             gTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTAt                                        <  2:1145922/149‑1 (MQ=255)
                                                                                                             gTTAATTCTTTTTCACCATCACTATCCCGGCCAAGTGTCGGGATTTTTTTCTTAATAAAAACGAAACTTAACAAGTACCAGGTATAAAATATGCCTAATGGACGAGCGGAAGAGTTTTCTGTATCCCTAAATGCACAAGTATTTTGTAt                                        >  1:3646780/1‑149 (MQ=255)
                                                                                                               tAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGc                                      >  2:1560076/1‑149 (MQ=255)
                                                                                                                 aTTCTTTTTCCCCATAACTATCCCGGCCATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGt                                    <  2:3529588/149‑1 (MQ=255)
                                                                                                                 aTTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGt                                    >  1:2432416/1‑149 (MQ=255)
                                                                                                                 aTTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGt                                    >  1:2432458/1‑149 (MQ=255)
                                                                                                                 aTTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGt                                    >  1:1001960/1‑149 (MQ=255)
                                                                                                                 aTTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGt                                    <  2:2941938/149‑1 (MQ=255)
                                                                                                                 aTTCTTTTTCACCAGCCCTAGCCCGGCCAGGTGTCGGTATTTTTTTCTTACAAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGt                                    <  1:2717348/149‑1 (MQ=255)
                                                                                                                  ttatttttCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTg                                   <  1:315298/146‑1 (MQ=255)
                                                                                                                   tattttaCACCAACACTAGCCCGGCCTGGTGTGGGTATTTTTTTCTTACAAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTgg                                  <  1:1055964/147‑1 (MQ=255)
                                                                                                                   tCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTgg                                  >  1:1606527/1‑149 (MQ=255)
                                                                                                                   tCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTgg                                  >  1:3688718/1‑149 (MQ=255)
                                                                                                                    cTTTTTCACCATCACTAGCCCGGCCATGTGTGGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGt                                 <  1:549296/149‑1 (MQ=255)
                                                                                                                      ttttCACCACCACTATCCCGGCCATGTGTCGGTATTTTTTTCTTACAAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGttt                               <  1:602409/149‑1 (MQ=255)
                                                                                                                         tCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTaa                            <  1:3056280/149‑1 (MQ=255)
                                                                                                                          cACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTaaa                           <  2:1510401/149‑1 (MQ=255)
                                                                                                                           aCCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAAt                          >  2:1861429/1‑149 (MQ=255)
                                                                                                                            ccATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAAtt                         <  2:1520262/149‑1 (MQ=255)
                                                                                                                                cACTAGCCCGGCCAGGTGGCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTAtt                     <  2:2187572/149‑1 (MQ=255)
                                                                                                                                       ccGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTg              <  2:3167177/149‑1 (MQ=255)
                                                                                                                                       ccGGCCAGGTGTCGTGATTTTTTTCTTACAAAACACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATGGATGGTg              <  2:1115587/149‑1 (MQ=255)
                                                                                                                                        cGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGa             >  1:2707735/1‑149 (MQ=255)
                                                                                                                                          gCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaat           <  2:3716612/149‑1 (MQ=255)
                                                                                                                                          gCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaat           >  2:1823540/1‑149 (MQ=255)
                                                                                                                                            cATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatat         >  1:2215053/1‑149 (MQ=255)
                                                                                                                                             aTGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatatt        <  2:20288/149‑1 (MQ=255)
                                                                                                                                             aTGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatatt        <  2:19938/149‑1 (MQ=255)
                                                                                                                                             aTGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatatt        >  2:524868/1‑149 (MQ=255)
                                                                                                                                              tgtgTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatatta       >  2:3013556/1‑149 (MQ=255)
                                                                                                                                              tgtgTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatatta       >  1:1328839/1‑149 (MQ=255)
                                                                                                                                               gtggCGGCATTTTTTTCTTACAAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatattaa      <  1:3183635/149‑1 (MQ=255)
                                                                                                                                                 gtCGGTATTTTTTTCTTAATAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatattaata    <  2:3793042/149‑1 (MQ=255)
                                                                                                                                                 gtCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatattaata    <  1:2954264/149‑1 (MQ=255)
                                                                                                                                                 gtCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGAGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatattaata    >  2:3118134/1‑149 (MQ=255)
                                                                                                                                                   cGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatattaatatt  >  1:2593620/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATT  >  CP009273/815931‑816226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑