Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 81,562 C→A 100% I236I (ATC→ATA)  leuO → global transcription factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927381,5620CA99.6% 890.9 / ‑4.4 265I236I (ATC→ATA) leuOglobal transcription factor
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (148/116);  total (148/117)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 267 total aligned reads displayed.

TGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAACAGCA  >  CP009273/81417‑81707
                                                                                                                                                 |                                                                                                                                                 
tGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATagcg                                                                                                                                                >  1:106590/1‑149 (MQ=255)
  gTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTa                                                                                                                                              <  1:2339791/149‑1 (MQ=255)
  gTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTa                                                                                                                                              >  2:3976673/1‑149 (MQ=255)
   tAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTAt                                                                                                                                             <  1:1964266/149‑1 (MQ=255)
     gCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCa                                                                                                                                           <  1:3173404/149‑1 (MQ=255)
      ccAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAg                                                                                                                                          >  1:1550581/1‑149 (MQ=255)
       cAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAgg                                                                                                                                         >  1:3500367/1‑149 (MQ=255)
           aaaaaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCAt                                                                                                                                     <  2:694872/149‑1 (MQ=255)
           aaaaaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCAt                                                                                                                                     <  1:2930588/149‑1 (MQ=255)
            aaaaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATg                                                                                                                                    >  1:2256183/1‑149 (MQ=255)
             aaaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATgg                                                                                                                                   >  2:2169977/1‑149 (MQ=255)
                tcatcCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCaa                                                                                                                                >  2:3855618/1‑149 (MQ=255)
                tcatcCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCaa                                                                                                                                >  2:1469798/1‑149 (MQ=255)
                   tcCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAatga                                                                                                                             <  1:2176901/149‑1 (MQ=255)
                     caacaaTTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAatgatg                                                                                                                           >  2:1542903/1‑149 (MQ=255)
                           ttAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTa                                                                                                                     <  2:1508805/149‑1 (MQ=255)
                            tAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTAc                                                                                                                    <  1:1719416/149‑1 (MQ=255)
                              aGGGCCCGTTACTGAAACATGATGTTTATCACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACtt                                                                                                                  <  1:1637228/149‑1 (MQ=255)
                                ggCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAg                                                                                                                <  2:301646/149‑1 (MQ=255)
                                     gTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCgtgg                                                                                                           >  2:3026060/1‑149 (MQ=255)
                                     gTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCgtgg                                                                                                           >  1:76807/1‑149 (MQ=255)
                                         cTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTc                                                                                                       <  1:3627037/149‑1 (MQ=255)
                                         cTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTc                                                                                                       <  2:827990/149‑1 (MQ=255)
                                          tGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCg                                                                                                      >  1:2060704/1‑149 (MQ=255)
                                          tGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCg                                                                                                      >  2:1125251/1‑149 (MQ=255)
                                           gAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGc                                                                                                     >  1:2723015/1‑149 (MQ=255)
                                            aaaCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCa                                                                                                    <  1:2334179/149‑1 (MQ=255)
                                              aCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCaaa                                                                                                  >  2:3643610/1‑149 (MQ=255)
                                              aCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCaaa                                                                                                  >  1:3696639/1‑149 (MQ=255)
                                              aCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCaaa                                                                                                  <  1:1160259/149‑1 (MQ=255)
                                              aCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCaaa                                                                                                  <  1:1011297/149‑1 (MQ=255)
                                                 tgatgTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGc                                                                                               >  2:3476623/1‑149 (MQ=255)
                                                 tgatgTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGc                                                                                               <  2:1742825/149‑1 (MQ=255)
                                                 ggatgTTTATAACGAACAACAGGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGAGAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGc                                                                                               <  2:2172178/148‑1 (MQ=255)
                                                    tgTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCAtt                                                                                            <  1:3982931/149‑1 (MQ=255)
                                                    tgTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCAtt                                                                                            <  2:864029/149‑1 (MQ=255)
                                                     gTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCAttt                                                                                           <  2:3884967/149‑1 (MQ=255)
                                                     gTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCAttt                                                                                           <  2:1845168/149‑1 (MQ=255)
                                                      tttATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTg                                                                                          >  1:568843/1‑149 (MQ=255)
                                                      tttATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTg                                                                                          >  1:568828/1‑149 (MQ=255)
                                                            aCGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCt                                                                                    <  2:106590/149‑1 (MQ=255)
                                                            aCGAACAACATGCGGCGGTTTCGCGCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCt                                                                                    >  1:3902146/1‑149 (MQ=255)
                                                                acaacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTg                                                                                <  1:3281989/149‑1 (MQ=255)
                                                                acaacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTg                                                                                >  1:1449931/1‑149 (MQ=255)
                                                                   acaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTgcgc                                                                             >  1:2412190/1‑149 (MQ=255)
                                                                     aTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGcgccg                                                                           <  1:4094712/149‑1 (MQ=255)
                                                                         ggcggTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGtt                                                                       >  2:3109406/1‑149 (MQ=255)
                                                                         ggcggTTTCGCTCGATCGTTTCGCGCCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGtt                                                                       <  1:576758/149‑1 (MQ=255)
                                                                            ggTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTtggc                                                                    <  2:296356/149‑1 (MQ=255)
                                                                             gTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTtggct                                                                   >  2:3279625/1‑149 (MQ=255)
                                                                             gTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTtggct                                                                   >  2:149216/1‑149 (MQ=255)
                                                                             gTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTtggct                                                                   <  1:1281781/149‑1 (MQ=255)
                                                                              tttCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTtggctg                                                                  <  2:3320267/149‑1 (MQ=255)
                                                                                tCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTtggctggc                                                                <  1:1273068/149‑1 (MQ=255)
                                                                                 cGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTggctggct                                                               >  2:4094119/1‑149 (MQ=255)
                                                                                   cTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGa                                                             >  1:1779021/1‑149 (MQ=255)
                                                                                    tCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGaa                                                            <  2:4071214/149‑1 (MQ=255)
                                                                                    tCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGaa                                                            >  2:3128325/1‑149 (MQ=255)
                                                                                       aTCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAagag                                                         >  1:3373102/1‑149 (MQ=255)
                                                                                       aTCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAagag                                                         >  1:1681061/1‑149 (MQ=255)
                                                                                       aTCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAagag                                                         <  1:2779298/149‑1 (MQ=255)
                                                                                            ttCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGc                                                    >  2:3571573/1‑149 (MQ=255)
                                                                                             tCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACTCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCt                                                   >  1:3008514/1‑149 (MQ=255)
                                                                                              cgcgTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTg                                                  >  1:841104/1‑149 (MQ=255)
                                                                                               gcgTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGACAGTATAGCGTATCAGGGCATGGCAATTATGAGCGTACTTAGCGTGGTGTCTCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGATTTCGATGa                                                 <  2:3358976/149‑1 (MQ=255)
                                                                                                cgTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGaa                                                <  2:450223/149‑1 (MQ=255)
                                                                                                    aTTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCt                                            >  1:2801233/1‑149 (MQ=255)
                                                                                                    aTTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCt                                            >  1:1750903/1‑149 (MQ=255)
                                                                                                      ttAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTa                                          <  2:2803281/149‑1 (MQ=255)
                                                                                                        aGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGa                                        >  1:2192720/1‑149 (MQ=255)
                                                                                                         gTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGaa                                       <  2:411353/149‑1 (MQ=255)
                                                                                                         gTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGaa                                       >  1:3709754/1‑149 (MQ=255)
                                                                                                         gTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGaa                                       >  2:3553301/1‑149 (MQ=255)
                                                                                                          tCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAAt                                      <  2:2723015/149‑1 (MQ=255)
                                                                                                            aaCCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTa                                    >  1:1824513/1‑149 (MQ=255)
                                                                                                              ccTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACa                                  >  2:693637/1‑149 (MQ=255)
                                                                                                               cTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAg                                 >  2:210526/1‑149 (MQ=255)
                                                                                                               cTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAg                                 <  2:35152/149‑1 (MQ=255)
                                                                                                               cTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAg                                 >  2:277239/1‑149 (MQ=255)
                                                                                                                ttGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAgg                                >  1:2765453/1‑149 (MQ=255)
                                                                                                                   gTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTAt                             <  2:2890573/149‑1 (MQ=255)
                                                                                                                       gACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTAcc                         <  2:3767395/149‑1 (MQ=255)
                                                                                                                       gACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTAcc                         <  1:3087556/149‑1 (MQ=255)
                                                                                                                        acacGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCg                        >  1:2673891/1‑149 (MQ=255)
                                                                                                                          acGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCt                      <  1:720930/149‑1 (MQ=255)
                                                                                                                            ggTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGc                    <  2:1279011/149‑1 (MQ=255)
                                                                                                                             gTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGcc                   >  1:380012/1‑149 (MQ=255)
                                                                                                                               aGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGt                 >  1:1257190/1‑149 (MQ=255)
                                                                                                                               aGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGt                 <  2:2339935/149‑1 (MQ=255)
                                                                                                                                  tAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaa              >  2:1336268/1‑149 (MQ=255)
                                                                                                                                    agcaagcCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaaaa            >  1:1175812/1‑149 (MQ=255)
                                                                                                                                     gcaagcCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaaaac           >  2:2701818/1‑149 (MQ=255)
                                                                                                                                       aagcCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaaaacaa         <  2:399592/149‑1 (MQ=255)
                                                                                                                                        agcCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaaaacaaa        >  1:2499121/1‑149 (MQ=255)
                                                                                                                                        agcCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaaaacaaa        <  1:441277/149‑1 (MQ=255)
                                                                                                                                         gcCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaaaacaaaa       >  1:1022102/1‑149 (MQ=255)
                                                                                                                                         gcCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaaaacaaaa       >  1:1598708/1‑149 (MQ=255)
                                                                                                                                           cAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAaaacaaaaca     <  2:1226004/149‑1 (MQ=255)
                                                                                                                                             gTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAAcagc   >  1:2582369/1‑149 (MQ=255)
                                                                                                                                             gTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAAcagc   <  1:3855618/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAACAGCA  >  CP009273/81417‑81707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑