Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 828,374 G→T 100% V11V (GTC→GTA)  ybiA ← DUF1768 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273828,3740GT100.0% 712.6 / NA 212V11V (GTC→GTA) ybiADUF1768 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (81/131);  total (81/131)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 212 total aligned reads displayed.

TTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGT  >  CP009273/828228‑828519
                                                                                                                                                  |                                                                                                                                                 
ttttCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTAc                                                                                                                                                 <  2:3446138/149‑1 (MQ=255)
  ttCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACgt                                                                                                                                               <  2:2170041/149‑1 (MQ=255)
    catcaAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTATAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACgtgt                                                                                                                                             <  1:4033956/149‑1 (MQ=255)
    catcaAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACgtgt                                                                                                                                             <  1:3377907/149‑1 (MQ=255)
    catcaAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACgtgt                                                                                                                                             <  1:65576/149‑1 (MQ=255)
     atcaAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGtt                                                                                                                                            <  2:2476529/149‑1 (MQ=255)
     atcaAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGtt                                                                                                                                            >  1:2061308/1‑149 (MQ=255)
     atcaAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGtt                                                                                                                                            <  2:1668758/149‑1 (MQ=255)
      tcaAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTg                                                                                                                                           <  1:1925437/149‑1 (MQ=255)
      tcaAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTg                                                                                                                                           >  2:1855569/1‑149 (MQ=255)
        aaGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGa                                                                                                                                         <  1:4075632/149‑1 (MQ=255)
        aaGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGa                                                                                                                                         <  1:2396760/149‑1 (MQ=255)
          ggAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATc                                                                                                                                       >  1:1685490/1‑149 (MQ=255)
            aaTTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCt                                                                                                                                     <  2:3570863/149‑1 (MQ=255)
             aTTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCtt                                                                                                                                    <  1:1597807/149‑1 (MQ=255)
             aTTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCtt                                                                                                                                    >  1:3072763/1‑149 (MQ=255)
              tttttGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCttt                                                                                                                                   <  2:2276823/149‑1 (MQ=255)
               ttttGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTtg                                                                                                                                  <  1:3463725/149‑1 (MQ=255)
               ttttGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTtg                                                                                                                                  <  1:3024546/149‑1 (MQ=255)
               ttttGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTtg                                                                                                                                  <  1:1543176/149‑1 (MQ=255)
                tttGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTtgt                                                                                                                                 <  2:1459704/149‑1 (MQ=255)
                        tGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAAc                                                                                                                         >  1:1330057/1‑149 (MQ=255)
                         gAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACg                                                                                                                        <  1:3485773/149‑1 (MQ=255)
                          aaaaTAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACgg                                                                                                                       >  2:3668985/1‑149 (MQ=255)
                          aaaaTAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACgg                                                                                                                       >  1:3162408/1‑149 (MQ=255)
                             aTAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCa                                                                                                                    >  1:3850374/1‑149 (MQ=255)
                                gtgtTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTa                                                                                                                 >  2:259483/1‑149 (MQ=255)
                                gtgtTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTa                                                                                                                 >  1:1397309/1‑149 (MQ=255)
                                  gtTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTaaa                                                                                                               <  2:2920609/149‑1 (MQ=255)
                                    tCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAAcc                                                                                                             >  2:910390/1‑149 (MQ=255)
                                    tCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAAcc                                                                                                             <  1:2966058/149‑1 (MQ=255)
                                    tCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAAcc                                                                                                             >  1:3600643/1‑149 (MQ=255)
                                     cTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCg                                                                                                            >  2:3173469/1‑149 (MQ=255)
                                      tGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGc                                                                                                           <  1:2027421/149‑1 (MQ=255)
                                        aGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCAt                                                                                                         <  1:1480815/149‑1 (MQ=255)
                                         ggTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCAtt                                                                                                        >  2:3724537/1‑149 (MQ=255)
                                         ggTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCAtt                                                                                                        >  2:938931/1‑149 (MQ=255)
                                            aGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATg                                                                                                     <  2:2478596/149‑1 (MQ=255)
                                             ggCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGt                                                                                                    >  2:3045064/1‑149 (MQ=255)
                                              gCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGtt                                                                                                   <  1:178764/149‑1 (MQ=255)
                                               ccAGGTGTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtg                                                                                                  <  2:2309152/149‑1 (MQ=255)
                                                   gTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtggtgg                                                                                              <  1:2706919/149‑1 (MQ=255)
                                                      ttCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTa                                                                                           <  1:1298908/149‑1 (MQ=255)
                                                       tCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAt                                                                                          <  2:1115628/149‑1 (MQ=255)
                                                       tCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAt                                                                                          <  1:1351857/149‑1 (MQ=255)
                                                       tCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAt                                                                                          >  2:3356758/1‑149 (MQ=255)
                                                        cccGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAtt                                                                                         <  1:1658047/149‑1 (MQ=255)
                                                        cccGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAtt                                                                                         <  1:489944/149‑1 (MQ=255)
                                                          cGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGc                                                                                       >  1:3085295/1‑149 (MQ=255)
                                                           gTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTTCg                                                                                      >  2:3482209/1‑149 (MQ=255)
                                                              aaCCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGc                                                                                   >  2:293009/1‑149 (MQ=255)
                                                               aCCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGcc                                                                                  >  2:2704057/1‑149 (MQ=255)
                                                                ccTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCg                                                                                 <  1:3754787/149‑1 (MQ=255)
                                                                 cTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGc                                                                                <  1:1623175/149‑1 (MQ=255)
                                                                  ttGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCt                                                                               <  1:353001/149‑1 (MQ=255)
                                                                    gATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCttt                                                                             >  2:2043257/1‑149 (MQ=255)
                                                                    gATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCttt                                                                             <  1:3173469/149‑1 (MQ=255)
                                                                           cAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaa                                                                      <  1:1113801/149‑1 (MQ=255)
                                                                            aGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaac                                                                     >  2:3957088/1‑149 (MQ=255)
                                                                            aGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaac                                                                     <  2:1754548/149‑1 (MQ=255)
                                                                                ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaacagaa                                                                 >  2:2856482/1‑149 (MQ=255)
                                                                                    aaGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAAc                                                             <  2:1263216/149‑1 (MQ=255)
                                                                                      gTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCa                                                           <  2:3802583/149‑1 (MQ=255)
                                                                                      gTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCa                                                           <  1:980274/149‑1 (MQ=255)
                                                                                       ttAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCAt                                                          >  2:1368842/1‑149 (MQ=255)
                                                                                               aTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATTATGGTGTCTTGCAATACGTGTTGGATCCTTTGTGCTCGAACTGGCATTAAACCGCATTATGTTGGTGTTTATAGAGAGACGATTTCCAGAAACAGAAAAAACATTAACCCTg                                                  >  2:2373826/1‑149 (MQ=255)
                                                                                               aTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTg                                                  >  2:1273850/1‑149 (MQ=255)
                                                                                                    cGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAAc                                             >  2:710840/1‑149 (MQ=255)
                                                                                                     gTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAAcc                                            <  1:3378651/149‑1 (MQ=255)
                                                                                                        gTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGaa                                         <  1:3695351/149‑1 (MQ=255)
                                                                                                         tCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGaaa                                        >  1:1053413/1‑149 (MQ=255)
                                                                                                          cATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCAGTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGaaaa                                       >  2:947034/1‑149 (MQ=255)
                                                                                                           aTCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGaaaaa                                      <  2:660293/149‑1 (MQ=255)
                                                                                                            tCGCTGGTGCTGTATAAATTTATGATGGTGTCTTGAATTAAGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAAt                                     <  2:1232163/149‑1 (MQ=255)
                                                                                                            tCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAAt                                     <  1:1243668/149‑1 (MQ=255)
                                                                                                            tCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAAt                                     <  2:3600643/149‑1 (MQ=255)
                                                                                                              gCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGc                                   <  2:4013025/149‑1 (MQ=255)
                                                                                                              gCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGc                                   <  1:3273594/149‑1 (MQ=255)
                                                                                                               cTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGcc                                  <  1:842720/149‑1 (MQ=255)
                                                                                                                tGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCca                                 <  2:717410/149‑1 (MQ=255)
                                                                                                                   tGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACaa                              <  2:1899057/149‑1 (MQ=255)
                                                                                                                      tGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAatat                           <  1:3051118/149‑1 (MQ=255)
                                                                                                                      tGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAatat                           <  1:1147306/149‑1 (MQ=255)
                                                                                                                      tGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAatat                           <  2:1330058/149‑1 (MQ=255)
                                                                                                                       gTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATAtt                          >  2:2108033/1‑149 (MQ=255)
                                                                                                                         aGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTgg                        >  2:2514858/1‑149 (MQ=255)
                                                                                                                         aGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTgg                        <  2:3072763/149‑1 (MQ=255)
                                                                                                                            aaTTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTg                     >  1:3253286/1‑149 (MQ=255)
                                                                                                                            aaTTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTg                     <  1:2798558/149‑1 (MQ=255)
                                                                                                                               ttATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGttt                  <  2:3085295/149‑1 (MQ=255)
                                                                                                                                tATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTta                 <  1:3430743/149‑1 (MQ=255)
                                                                                                                                    atgGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACa             <  1:1344191/149‑1 (MQ=255)
                                                                                                                                       gtgtCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTa          <  1:1855569/149‑1 (MQ=255)
                                                                                                                                        tgtCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTAt         >  1:1695773/1‑149 (MQ=255)
                                                                                                                                         gtCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTAtt        >  2:3386139/1‑149 (MQ=255)
                                                                                                                                          tCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTAttt       >  1:2558999/1‑149 (MQ=255)
                                                                                                                                           cTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTc      <  1:2995957/149‑1 (MQ=255)
                                                                                                                                             tGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAg    >  1:1842832/1‑149 (MQ=255)
                                                                                                                                              gCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAgg   >  2:3162005/1‑149 (MQ=255)
                                                                                                                                               cATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGt  <  2:1811127/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGT  >  CP009273/828228‑828519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑