Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 842,041 C→A 100% E179* (GAA→TAA)  glnP ← glutamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273842,0410CA96.4% 661.4 / 14.5 222E179* (GAA→TAA) glnPglutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (4/4);  new base A (105/109);  total (109/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 222 total aligned reads displayed.

GACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGT  >  CP009273/841899‑842186
                                                                                                                                              |                                                                                                                                                 
gaCGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGccc                                                                                                                                             >  2:1806059/1‑149 (MQ=255)
gaCGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGccc                                                                                                                                             >  2:3832007/1‑149 (MQ=255)
  cGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCt                                                                                                                                           <  1:3834407/149‑1 (MQ=255)
     ttttAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGAc                                                                                                                                        <  2:734846/149‑1 (MQ=255)
     ttttAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGAc                                                                                                                                        <  1:1277568/149‑1 (MQ=255)
       ttAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACgg                                                                                                                                      <  1:2530062/149‑1 (MQ=255)
        tAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACggg                                                                                                                                     <  2:3222559/149‑1 (MQ=255)
        tAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACggg                                                                                                                                     <  1:375011/149‑1 (MQ=255)
         aaaTTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGt                                                                                                                                    >  1:1091524/1‑149 (MQ=255)
         aaaTTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGt                                                                                                                                    >  2:1139496/1‑149 (MQ=255)
             tCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGt                                                                                                                                <  1:3570043/149‑1 (MQ=255)
             tCAATCACAGGATTTTCAGCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGt                                                                                                                                >  2:2105350/1‑149 (MQ=255)
                 tCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCg                                                                                                                            >  2:553496/1‑149 (MQ=255)
                   acaGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGcc                                                                                                                          >  2:12361/1‑149 (MQ=255)
                   acaGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGcc                                                                                                                          >  2:1267201/1‑149 (MQ=255)
                   acaGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGcc                                                                                                                          >  1:2751187/1‑149 (MQ=255)
                    caGGATTTTCATCCGTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATACTCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCa                                                                                                                         <  1:1771933/149‑1 (MQ=255)
                       gATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGc                                                                                                                      <  2:143868/149‑1 (MQ=255)
                        aTTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGcc                                                                                                                     <  2:2880587/149‑1 (MQ=255)
                        aTTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGcc                                                                                                                     <  1:626361/149‑1 (MQ=255)
                         ttttCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCg                                                                                                                    >  1:1493451/1‑149 (MQ=255)
                          tttCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGa                                                                                                                   <  2:2648004/149‑1 (MQ=255)
                          tttCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGa                                                                                                                   >  2:3546004/1‑149 (MQ=255)
                           ttCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGAt                                                                                                                  <  2:492697/149‑1 (MQ=255)
                            tCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATc                                                                                                                 <  2:4037357/149‑1 (MQ=255)
                             cATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATca                                                                                                                <  1:1437269/149‑1 (MQ=255)
                             cATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATca                                                                                                                <  1:498951/149‑1 (MQ=255)
                               tCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATcaca                                                                                                              >  1:871854/1‑149 (MQ=255)
                               tCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATcaca                                                                                                              >  1:1184852/1‑149 (MQ=255)
                                ccTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACaa                                                                                                             <  1:2252767/149‑1 (MQ=255)
                                   tcttTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATaa                                                                                                          <  1:3498232/149‑1 (MQ=255)
                                   tcttTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATaa                                                                                                          <  1:3512626/149‑1 (MQ=255)
                                     tttCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAAc                                                                                                        >  2:1424207/1‑149 (MQ=255)
                                     tttCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAAc                                                                                                        >  2:3473182/1‑149 (MQ=255)
                                       gccAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAg                                                                                                      <  2:3907472/148‑1 (MQ=255)
                                         cAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCg                                                                                                    >  1:1313497/1‑149 (MQ=255)
                                           gacgacGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAg                                                                                                  >  1:950063/1‑149 (MQ=255)
                                            acgacgCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAgg                                                                                                 >  1:1339972/1‑149 (MQ=255)
                                             cgacgCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGgt                                                                                                <  2:191619/149‑1 (MQ=255)
                                             cgacgCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGgt                                                                                                <  1:3639598/149‑1 (MQ=255)
                                             cgacgCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGgt                                                                                                <  1:3795271/149‑1 (MQ=255)
                                              gacgCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGgtg                                                                                               <  1:774215/149‑1 (MQ=255)
                                              gacgCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATGTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGgtg                                                                                               >  2:2091263/1‑149 (MQ=255)
                                               acgCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGgtgt                                                                                              <  2:2448040/149‑1 (MQ=255)
                                                  cAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCtt                                                                                           <  1:1998595/149‑1 (MQ=255)
                                                  cAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCtt                                                                                           <  2:782549/149‑1 (MQ=255)
                                                     aaTAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTaa                                                                                        >  2:3704100/1‑149 (MQ=255)
                                                     aaTAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTaa                                                                                        <  2:3054377/149‑1 (MQ=255)
                                                      aTAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAAt                                                                                       <  2:2823169/149‑1 (MQ=255)
                                                       tAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATg                                                                                      >  2:3276629/1‑149 (MQ=255)
                                                         aaGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCt                                                                                    >  1:422986/1‑149 (MQ=255)
                                                         aaGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCt                                                                                    >  2:3948819/1‑149 (MQ=255)
                                                         aaGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCt                                                                                    >  2:2697647/1‑149 (MQ=255)
                                                           gCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCtga                                                                                  <  2:578112/149‑1 (MQ=255)
                                                              cAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCtgatga                                                                               <  1:1531534/149‑1 (MQ=255)
                                                               aGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTgatgat                                                                              <  2:1889664/149‑1 (MQ=255)
                                                               aGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTgatgat                                                                              <  2:3144766/149‑1 (MQ=255)
                                                               aGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTgatgat                                                                              <  2:1889572/149‑1 (MQ=255)
                                                                  aCCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCa                                                                           >  2:1537532/1‑149 (MQ=255)
                                                                          aataatCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTAc                                                                   <  1:1007156/149‑1 (MQ=255)
                                                                           ataatCAGATAGAACACCGCCCCTGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTAcc                                                                  <  2:1185380/149‑1 (MQ=255)
                                                                           ataatCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTAcc                                                                  >  2:4103183/1‑149 (MQ=255)
                                                                           ataatCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTAcc                                                                  >  2:2705465/1‑149 (MQ=255)
                                                                              atCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAg                                                               >  1:1221184/1‑149 (MQ=255)
                                                                              atCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAg                                                               >  2:526622/1‑149 (MQ=255)
                                                                                cAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAgcg                                                             <  1:1267201/149‑1 (MQ=255)
                                                                                    tAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGcggcgg                                                         >  1:3670893/1‑149 (MQ=255)
                                                                                      gAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGgca                                                       >  2:3117771/1‑149 (MQ=255)
                                                                                       aaCACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGgcag                                                      >  2:4115080/1‑149 (MQ=255)
                                                                                        acacCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGgcagc                                                     >  1:1588708/1‑149 (MQ=255)
                                                                                        acacCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGgcagc                                                     >  1:235872/1‑149 (MQ=255)
                                                                                         cacCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGgcagca                                                    <  1:1385597/149‑1 (MQ=255)
                                                                                          acCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACGCAGCGGCGGCAGCAt                                                   <  1:955648/149‑1 (MQ=255)
                                                                                                aCGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATacgacg                                             <  1:1874162/149‑1 (MQ=255)
                                                                                                 cGGCGCTACAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGTCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGc                                            <  1:339278/149‑1 (MQ=255)
                                                                                                   gcgcTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAg                                          <  1:293705/149‑1 (MQ=255)
                                                                                                   gcgcTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAg                                          <  2:1316776/149‑1 (MQ=255)
                                                                                                     gcTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTg                                        >  1:1888896/1‑149 (MQ=255)
                                                                                                     gcTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTg                                        >  1:3841175/1‑149 (MQ=255)
                                                                                                              ctcAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTa                               <  1:2099046/149‑1 (MQ=255)
                                                                                                               tcAAGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTaa                              >  2:2333906/1‑149 (MQ=255)
                                                                                                                 aaGGGCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTaaaa                            <  1:3276629/149‑1 (MQ=255)
                                                                                                                    ggCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGa                         <  1:1806059/149‑1 (MQ=255)
                                                                                                                    ggCGCGGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGa                         >  2:3632439/1‑149 (MQ=255)
                                                                                                                       gcgGAAGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTACCGCCACGCCTATCACAATAAACAGCGAGGTGTCTTTAAGGCTGATGATCAACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGt                      <  1:1840858/149‑1 (MQ=255)
                                                                                                                           aaGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCg                  <  2:1180594/149‑1 (MQ=255)
                                                                                                                            aGTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGa                 >  1:674202/1‑149 (MQ=255)
                                                                                                                             gTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGaa                <  2:1091524/149‑1 (MQ=255)
                                                                                                                             gTTACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGaa                <  2:620835/149‑1 (MQ=255)
                                                                                                                               tACCGGCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATg              >  2:3303538/1‑149 (MQ=255)
                                                                                                                                   ggCAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGttt          >  2:248505/1‑149 (MQ=255)
                                                                                                                                    gCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTc         >  1:2590809/1‑149 (MQ=255)
                                                                                                                                     cAATAATTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTcc        <  1:3580949/149‑1 (MQ=255)
                                                                                                                                      aataatTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTccc       >  2:1638845/1‑149 (MQ=255)
                                                                                                                                      aataatTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTccc       <  1:1798314/149‑1 (MQ=255)
                                                                                                                                         aatTTATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAAc    <  2:1283275/149‑1 (MQ=255)
                                                                                                                                           tttATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGt  >  1:2494076/1‑149 (MQ=255)
                                                                                                                                           tttATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGt  >  1:1247546/1‑149 (MQ=255)
                                                                                                                                           tttATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGt  <  2:1493453/149‑1 (MQ=255)
                                                                                                                                           tttATTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGt  >  1:1137183/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
GACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGT  >  CP009273/841899‑842186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑