Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 882,672 G→T 100% L42I (CTC→ATC)  ybjJ ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273882,6720GT98.4% 819.3 / 3.2 257L42I (CTC→ATC) ybjJputative transporter
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (131/122);  total (132/125)
Fisher's exact test for biased strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 271 total aligned reads displayed.

CCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGA  >  CP009273/882526‑882817
                                                                                                                                                  |                                                                                                                                                 
cccGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATaa                                                                                                                                                 <  1:83150/149‑1 (MQ=255)
 ccGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAat                                                                                                                                                <  2:3726792/149‑1 (MQ=255)
 ccGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAat                                                                                                                                                >  2:2810539/1‑149 (MQ=255)
 ccGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAat                                                                                                                                                <  1:4088684/149‑1 (MQ=255)
   gATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAatat                                                                                                                                              <  2:1459721/149‑1 (MQ=255)
    aTCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATc                                                                                                                                             >  2:4073179/1‑149 (MQ=255)
     tCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGAGCGAGACAGAGATAATATcg                                                                                                                                            >  2:2668408/1‑149 (MQ=255)
        aTGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGCTCGAGACAGAGATAATATCGCgg                                                                                                                                         >  2:2545707/1‑149 (MQ=255)
        aTGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCgg                                                                                                                                         >  1:1716830/1‑149 (MQ=255)
        aTGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCgg                                                                                                                                         >  1:1834718/1‑149 (MQ=255)
        aTGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCgg                                                                                                                                         >  1:4102096/1‑149 (MQ=255)
         tGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACAACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGAGGa                                                                                                                                        >  2:3195281/1‑149 (MQ=255)
             cAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGc                                                                                                                                    >  2:2551155/1‑149 (MQ=255)
              aGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCAGTTCAGCGATCGAGACCGAGATAATATCGCGGATAGCa                                                                                                                                   >  2:3474245/1‑149 (MQ=255)
               ggACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAg                                                                                                                                  <  1:492034/149‑1 (MQ=255)
               ggACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAg                                                                                                                                  <  2:3814134/149‑1 (MQ=255)
                    tCGTGACCAGGATGACATTACGTGTCCCACAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTa                                                                                                                             <  1:2771990/149‑1 (MQ=255)
                    tCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTa                                                                                                                             <  1:3741745/149‑1 (MQ=255)
                    tCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTa                                                                                                                             <  1:2472350/149‑1 (MQ=255)
                       tGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACgg                                                                                                                          >  1:3165179/1‑149 (MQ=255)
                        gACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACggg                                                                                                                         <  1:2425981/149‑1 (MQ=255)
                             ggATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGcc                                                                                                                    <  1:635259/149‑1 (MQ=255)
                                tGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAg                                                                                                                 >  2:1758444/1‑149 (MQ=255)
                                       aCGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCa                                                                                                          >  2:3430825/1‑149 (MQ=255)
                                       aCGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCa                                                                                                          <  1:1007774/149‑1 (MQ=255)
                                       aCGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCa                                                                                                          <  1:1007728/149‑1 (MQ=255)
                                        cGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCAt                                                                                                         <  2:2047653/149‑1 (MQ=255)
                                           gtCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTaa                                                                                                      >  2:1545042/1‑149 (MQ=255)
                                           gtCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTaa                                                                                                      <  2:1196899/149‑1 (MQ=255)
                                           gtCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTaa                                                                                                      <  1:1430482/149‑1 (MQ=255)
                                              ccAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCNCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAg                                                                                                   <  1:2082854/149‑1 (MQ=255)
                                              ccAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAg                                                                                                   >  1:1260088/1‑149 (MQ=255)
                                              ccAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAg                                                                                                   <  1:465512/149‑1 (MQ=255)
                                               cAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCACAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAgg                                                                                                  <  1:1241415/149‑1 (MQ=255)
                                               cAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAgg                                                                                                  >  1:3864285/1‑149 (MQ=255)
                                                 aaGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGcc                                                                                                <  1:2228296/149‑1 (MQ=255)
                                                 aaGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGcc                                                                                                <  2:1642298/149‑1 (MQ=255)
                                                 aaGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGcc                                                                                                >  2:107075/1‑149 (MQ=255)
                                                 aaGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGcc                                                                                                <  2:4111871/149‑1 (MQ=255)
                                                 aaGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGcc                                                                                                <  2:3877068/149‑1 (MQ=255)
                                                  aGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCt                                                                                               >  2:1032874/1‑149 (MQ=255)
                                                   gCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTg                                                                                              >  2:4051524/1‑149 (MQ=255)
                                                      tttCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCAGTCACAGGCCTGGCa                                                                                           >  1:428589/1‑149 (MQ=255)
                                                       ttCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGCTCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTCACAGGCCTGGcca                                                                                          >  2:3879372/1‑147 (MQ=255)
                                                         cACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCaaaa                                                                                        <  1:3323227/149‑1 (MQ=255)
                                                          aCTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCaaaaa                                                                                       >  2:1219509/1‑149 (MQ=255)
                                                           cTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaag                                                                                      >  1:167472/1‑149 (MQ=255)
                                                             aaCCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaagaa                                                                                    >  2:266523/1‑149 (MQ=255)
                                                              aCCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaagaag                                                                                   >  2:2068864/1‑149 (MQ=255)
                                                              aCCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaagaag                                                                                   >  1:3052812/1‑149 (MQ=255)
                                                              aCCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaagaag                                                                                   >  2:2068099/1‑149 (MQ=255)
                                                               ccACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAagaaga                                                                                  >  2:2166848/1‑149 (MQ=255)
                                                                cACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCCTTAACAGGCCTGGCAAAAAgaagaa                                                                                 >  1:1080388/1‑149 (MQ=255)
                                                                 aCGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAAc                                                                                >  1:357299/1‑149 (MQ=255)
                                                                  cGCCGAGCAGAGAATACCGCTTATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACa                                                                               <  2:3979597/149‑1 (MQ=255)
                                                                      gAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATaaa                                                                           >  1:1316522/1‑149 (MQ=255)
                                                                      gAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATaaa                                                                           <  2:2278577/149‑1 (MQ=255)
                                                                       aGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAAc                                                                          <  2:1398254/149‑1 (MQ=255)
                                                                       aGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAAc                                                                          <  1:29720/149‑1 (MQ=255)
                                                                        gCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACa                                                                         >  2:794683/1‑149 (MQ=255)
                                                                              aaTACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGccc                                                                   >  1:2709893/1‑149 (MQ=255)
                                                                               aTACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCa                                                                  >  2:415325/1‑149 (MQ=255)
                                                                               aTACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCa                                                                  >  2:2705692/1‑149 (MQ=255)
                                                                                tACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAg                                                                 >  2:2263328/1‑149 (MQ=255)
                                                                                 aCCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAgg                                                                <  2:723396/149‑1 (MQ=255)
                                                                                   cGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGtt                                                              <  2:1699149/149‑1 (MQ=255)
                                                                                    gCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTc                                                             <  1:129042/149‑1 (MQ=255)
                                                                                      tCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCgg                                                           >  1:2238475/1‑149 (MQ=255)
                                                                                        aTCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCg                                                         >  1:1212852/1‑149 (MQ=255)
                                                                                            aaCCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTc                                                     <  1:2551155/149‑1 (MQ=255)
                                                                                            aaCCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTc                                                     >  1:498836/1‑149 (MQ=255)
                                                                                              ccGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCaa                                                   <  2:1442808/149‑1 (MQ=255)
                                                                                                     aCAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGGAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTa                                            >  2:3898870/1‑149 (MQ=255)
                                                                                                     aCAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTa                                            >  2:1513445/1‑149 (MQ=255)
                                                                                                     aCAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTa                                            <  1:652935/149‑1 (MQ=255)
                                                                                                            aaaGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTgaag                                     <  1:205063/149‑1 (MQ=255)
                                                                                                               gagaACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAAt                                  >  2:2137910/1‑149 (MQ=255)
                                                                                                               gagaACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAAt                                  >  1:2400945/1‑149 (MQ=255)
                                                                                                                agaACACCGCCCTTTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAAtt                                 <  1:2848515/149‑1 (MQ=255)
                                                                                                                   acacCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTAc                              <  2:3753319/149‑1 (MQ=255)
                                                                                                                   acacCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTAc                              <  2:3753413/149‑1 (MQ=255)
                                                                                                                   acacCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGCACGCCATTAACAGGCCTGCAAAAAAGAAGAACATAACCAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTAc                              <  1:1757592/149‑1 (MQ=255)
                                                                                                                     acCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACgg                            <  2:3854486/149‑1 (MQ=255)
                                                                                                                           cATTTCAGCGATCGAGACCGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAg                      >  2:610426/1‑149 (MQ=255)
                                                                                                                           cATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAg                      >  2:2675068/1‑149 (MQ=255)
                                                                                                                            aTTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGa                     <  2:2182289/149‑1 (MQ=255)
                                                                                                                             tttCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGAt                    <  2:569525/149‑1 (MQ=255)
                                                                                                                             tttCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGAt                    <  2:1885355/149‑1 (MQ=255)
                                                                                                                              ttCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATc                   <  2:1716972/149‑1 (MQ=255)
                                                                                                                                     aTCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCaa            >  2:616309/1‑149 (MQ=255)
                                                                                                                                     aTCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAAACCATTACGTGAATAATTTACGGTCATAGATCACGTCaa            >  2:2959005/1‑149 (MQ=255)
                                                                                                                                        gagaCAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAAt         <  1:2310783/149‑1 (MQ=255)
                                                                                                                                         agaCAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCACAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATa        >  2:3538291/1‑149 (MQ=255)
                                                                                                                                         agaCAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATa        >  1:1574708/1‑149 (MQ=255)
                                                                                                                                            cAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATaaga     >  1:193373/1‑149 (MQ=255)
                                                                                                                                              gagataATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAATATaagaag   >  1:963645/1‑149 (MQ=255)
                                                                                                                                              gagataATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATaagaag   <  2:2483565/149‑1 (MQ=255)
                                                                                                                                               agataATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAagaaga  >  2:1634288/1‑149 (MQ=255)
                                                                                                                                               agataATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAagaaga  >  1:4036671/1‑149 (MQ=255)
                                                                                                                                               agataATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAagaaga  <  1:609105/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGA  >  CP009273/882526‑882817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑