Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 7,522 G→T 100% F146L (TTC→TTA)  yaaJ ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092737,5220GT98.8% 829.5 / 0.6 257F146L (TTC→TTA) yaaJputative transporter
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (134/120);  total (135/122)
Fisher's exact test for biased strand distribution p-value = 6.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 257 total aligned reads displayed.

GAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGG  >  CP009273/7377‑7667
                                                                                                                                                 |                                                                                                                                                 
gAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAAc                                                                                                                                                <  2:1201399/149‑1 (MQ=255)
    taTTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGaa                                                                                                                                            <  2:788732/148‑1 (MQ=255)
    gATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGaa                                                                                                                                            <  2:1614697/149‑1 (MQ=255)
       tGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGc                                                                                                                                         <  2:2154934/149‑1 (MQ=255)
          cagcagAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGTAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGATAACAGAACGCCCa                                                                                                                                      <  1:3490203/149‑1 (MQ=255)
              agagTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCa                                                                                                                                  >  1:3487573/1‑149 (MQ=255)
                   aaaGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCa                                                                                                                             >  1:1238452/1‑149 (MQ=255)
                   aaaGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCa                                                                                                                             >  2:137447/1‑149 (MQ=255)
                   aaaGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCa                                                                                                                             >  2:137178/1‑149 (MQ=255)
                      gACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATcc                                                                                                                          >  2:2237504/1‑149 (MQ=255)
                        cAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATcccc                                                                                                                        <  1:2187476/149‑1 (MQ=255)
                         aGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCa                                                                                                                       <  1:1048140/149‑1 (MQ=255)
                         aGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCa                                                                                                                       <  2:634810/149‑1 (MQ=255)
                           cGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGc                                                                                                                     <  1:3585461/149‑1 (MQ=255)
                            gagaATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGcc                                                                                                                    >  1:101717/1‑149 (MQ=255)
                                ataatGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcg                                                                                                                >  2:3363263/1‑149 (MQ=255)
                                 taatGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcgc                                                                                                               >  2:3201611/1‑149 (MQ=255)
                                  aatGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcgcg                                                                                                              >  2:3525013/1‑149 (MQ=255)
                                     gCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCa                                                                                                           >  2:2872979/1‑149 (MQ=255)
                                       cTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCata                                                                                                         >  1:414040/1‑149 (MQ=255)
                                               aGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGc                                                                                                 >  1:888107/1‑149 (MQ=255)
                                               aGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGc                                                                                                 >  1:1518765/1‑149 (MQ=255)
                                                gCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGcc                                                                                                >  1:195835/1‑149 (MQ=255)
                                                  gggggAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCgg                                                                                              >  1:2121022/1‑149 (MQ=255)
                                                  gggggAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCgg                                                                                              >  1:4036683/1‑149 (MQ=255)
                                                    gggAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTc                                                                                            <  2:1735229/149‑1 (MQ=255)
                                                    gggAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTc                                                                                            >  1:3360144/1‑149 (MQ=255)
                                                     ggAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTcc                                                                                           >  2:2650954/1‑149 (MQ=255)
                                                     ggAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTcc                                                                                           >  1:3608644/1‑149 (MQ=255)
                                                     ggAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTcc                                                                                           >  1:3306760/1‑149 (MQ=255)
                                                      gAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCg                                                                                          >  1:2843532/1‑149 (MQ=255)
                                                         aaTCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCa                                                                                       >  2:2787303/1‑149 (MQ=255)
                                                         aaTCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCa                                                                                       >  2:2410723/1‑149 (MQ=255)
                                                          aTCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGATCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGAAAAAATACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACAATGCAGGTCCGCAAc                                                                                      >  2:227244/1‑149 (MQ=255)
                                                             aaaaGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGaa                                                                                   >  1:3629153/1‑149 (MQ=255)
                                                               aaGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAAc                                                                                 <  1:4017415/149‑1 (MQ=255)
                                                                    aaaCTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGccc                                                                            <  1:3877798/149‑1 (MQ=255)
                                                                       cTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCAtt                                                                         >  2:2011793/1‑149 (MQ=255)
                                                                       cTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCAtt                                                                         <  1:1135049/149‑1 (MQ=255)
                                                                        tCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTg                                                                        <  1:4008785/149‑1 (MQ=255)
                                                                         cAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGa                                                                       <  1:3216204/149‑1 (MQ=255)
                                                                         cAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGa                                                                       <  1:1822455/149‑1 (MQ=255)
                                                                          aGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCAGTGAc                                                                      >  2:1763694/1‑149 (MQ=255)
                                                                          aGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTCAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCCTGCCGGTCCGCCACGAAACGGCCCATTGAc                                                                      >  2:2776826/1‑149 (MQ=255)
                                                                           gggCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACg                                                                     >  1:2351722/1‑149 (MQ=255)
                                                                           gggCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACg                                                                     >  1:2890101/1‑149 (MQ=255)
                                                                            ggCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGt                                                                    >  1:3422347/1‑149 (MQ=255)
                                                                            ggCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGt                                                                    >  1:300963/1‑149 (MQ=255)
                                                                            ggCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGt                                                                    <  1:2707501/149‑1 (MQ=255)
                                                                             gcgcGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTc                                                                   <  1:3274390/149‑1 (MQ=255)
                                                                             gcgcGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTc                                                                   <  2:1262747/149‑1 (MQ=255)
                                                                               gcgGTCTACGGCGTTAGCTTGAACTCCGCTGAAAATTATGACATAGGCGATGAGCAAAAAGACGGCTAACTGTACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCAGGCCGGTCCGCCACGAAACTGCCCTTTGACGTCAc                                                                 <  2:3759941/149‑1 (MQ=255)
                                                                               gcgGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCAc                                                                 <  1:1981126/149‑1 (MQ=255)
                                                                                  ggCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGtt                                                              >  2:3029395/1‑149 (MQ=255)
                                                                                   gCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTc                                                             <  1:1667439/149‑1 (MQ=255)
                                                                                    cAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCt                                                            <  2:345948/149‑1 (MQ=255)
                                                                                    cAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCt                                                            <  1:2391403/149‑1 (MQ=255)
                                                                                        ggCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTtat                                                        <  1:700184/149‑1 (MQ=255)
                                                                                         gCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTtata                                                       >  1:860887/1‑149 (MQ=255)
                                                                                           gTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATaaa                                                     <  1:1457856/149‑1 (MQ=255)
                                                                                           gTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATaaa                                                     <  2:389884/149‑1 (MQ=255)
                                                                                            ttCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAg                                                    <  2:2677424/149‑1 (MQ=255)
                                                                                            ttCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAg                                                    >  2:2516696/1‑149 (MQ=255)
                                                                                             tCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGt                                                   <  1:2237815/149‑1 (MQ=255)
                                                                                               gCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTtg                                                 <  2:2262806/149‑1 (MQ=255)
                                                                                                 ttGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTtgtg                                               >  2:3554961/1‑149 (MQ=255)
                                                                                                 ttGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTtgtg                                               >  2:2715711/1‑149 (MQ=255)
                                                                                                 ttGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTtgtg                                               >  2:183748/1‑149 (MQ=255)
                                                                                                 ttGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTtgtg                                               >  2:1845614/1‑149 (MQ=255)
                                                                                                 ttGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTtgtg                                               >  1:162355/1‑149 (MQ=255)
                                                                                                   gAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGcc                                             <  1:4079919/149‑1 (MQ=255)
                                                                                                   gAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGcc                                             <  1:3144258/149‑1 (MQ=255)
                                                                                                    aaCTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCa                                            <  1:2236347/149‑1 (MQ=255)
                                                                                                    aaCTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCa                                            <  2:2318058/149‑1 (MQ=255)
                                                                                                      cTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCaga                                          <  1:2038098/149‑1 (MQ=255)
                                                                                                      cTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCaga                                          <  2:640948/149‑1 (MQ=255)
                                                                                                      cTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCaga                                          >  2:446710/1‑149 (MQ=255)
                                                                                                       tCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCagag                                         <  2:524030/149‑1 (MQ=255)
                                                                                                         cGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGaa                                       >  1:3523513/1‑149 (MQ=255)
                                                                                                          gCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAAc                                      >  1:581994/1‑149 (MQ=255)
                                                                                                           cTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACa                                     <  1:1786799/149‑1 (MQ=255)
                                                                                                           cTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACa                                     <  1:303948/149‑1 (MQ=255)
                                                                                                             gAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACAtt                                   <  1:2800596/149‑1 (MQ=255)
                                                                                                              aaaaTTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTc                                  >  1:2641058/1‑149 (MQ=255)
                                                                                                                  ttATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCa                              >  1:1969105/1‑149 (MQ=255)
                                                                                                                  ttATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCa                              >  1:1707518/1‑149 (MQ=255)
                                                                                                                     tGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAAc                           <  1:2936164/149‑1 (MQ=255)
                                                                                                                           aGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGGTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTc                     <  1:50930/149‑1 (MQ=255)
                                                                                                                               gATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGccc                 >  2:3977924/1‑148 (MQ=255)
                                                                                                                               gATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCa                 <  1:532149/149‑1 (MQ=255)
                                                                                                                                aTGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCAt                <  2:2351722/149‑1 (MQ=255)
                                                                                                                                  gAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGc              <  2:1488830/149‑1 (MQ=255)
                                                                                                                                  gAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGc              >  1:2623522/1‑149 (MQ=255)
                                                                                                                                     cAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGa           <  2:1455994/149‑1 (MQ=255)
                                                                                                                                      aaaaaGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGAt          <  1:1260497/149‑1 (MQ=255)
                                                                                                                                        aaaGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATaa        >  1:1363442/1‑149 (MQ=255)
                                                                                                                                         aaGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATaaa       >  2:3123977/1‑149 (MQ=255)
                                                                                                                                         aaGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATaaa       <  2:3608621/149‑1 (MQ=255)
                                                                                                                                           gACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAAcg     >  1:525101/1‑149 (MQ=255)
                                                                                                                                              ggCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCgg  >  2:100554/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGG  >  CP009273/7377‑7667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑