Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 997,673 A→T 100% T137T (ACA→ACT)  elfG → putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273997,6730AT99.6% 890.0 / ‑4.8 269T137T (ACA→ACT) elfGputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (157/111);  total (158/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 269 total aligned reads displayed.

AGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTATGGCGCTGG  >  CP009273/997527‑997815
                                                                                                                                                  |                                                                                                                                              
aGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTgg                                                                                                                                              <  1:2781359/149‑1 (MQ=255)
 gTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTggg                                                                                                                                             >  2:357435/1‑149 (MQ=255)
 gTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTggg                                                                                                                                             >  2:1079811/1‑149 (MQ=255)
  tGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTgggg                                                                                                                                            <  1:294140/149‑1 (MQ=255)
    gCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTgggggg                                                                                                                                          >  1:105322/1‑149 (MQ=255)
       gATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGAt                                                                                                                                       >  1:2536963/1‑149 (MQ=255)
        aTGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATa                                                                                                                                      >  2:116457/1‑149 (MQ=255)
          gggCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATAcc                                                                                                                                    >  1:2502018/1‑149 (MQ=255)
            gCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCg                                                                                                                                  >  2:2319234/1‑149 (MQ=255)
            gCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCg                                                                                                                                  >  2:1310319/1‑149 (MQ=255)
              aTTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGAt                                                                                                                                >  1:1673518/1‑149 (MQ=255)
               ttCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATg                                                                                                                               >  1:2731338/1‑149 (MQ=255)
                  gccgcGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCa                                                                                                                            <  2:1986732/149‑1 (MQ=255)
                   ccgcGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCat                                                                                                                           <  2:3374401/149‑1 (MQ=255)
                    cgcgGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCata                                                                                                                          >  1:1066191/1‑149 (MQ=255)
                        gATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTg                                                                                                                      >  2:776659/1‑149 (MQ=255)
                            cACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGAc                                                                                                                  >  1:2618439/1‑149 (MQ=255)
                              ccGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGAccc                                                                                                                <  1:1431838/149‑1 (MQ=255)
                              ccGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGAccc                                                                                                                >  1:2207516/1‑149 (MQ=255)
                              ccGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGAccc                                                                                                                <  2:1503856/149‑1 (MQ=255)
                                 ttAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTgg                                                                                                             >  2:1424019/1‑149 (MQ=255)
                                     tCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt                                                                                                         >  1:2015439/1‑149 (MQ=255)
                                       tATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCa                                                                                                       >  2:3510249/1‑149 (MQ=255)
                                        aTTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCaa                                                                                                      <  1:4108237/149‑1 (MQ=255)
                                         tttAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAAc                                                                                                     >  1:3741614/1‑149 (MQ=255)
                                           tAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGt                                                                                                   <  1:3660873/149‑1 (MQ=255)
                                           tAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGt                                                                                                   >  1:3332497/1‑149 (MQ=255)
                                               cGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAg                                                                                               >  1:61291/1‑149 (MQ=255)
                                                  cAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTg                                                                                            <  2:1246046/149‑1 (MQ=255)
                                                   aaCTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTgg                                                                                           >  2:1718271/1‑149 (MQ=255)
                                                    aCTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTggg                                                                                          >  1:874671/1‑149 (MQ=255)
                                                       ggTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAg                                                                                       <  1:697332/149‑1 (MQ=255)
                                                       ggTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAg                                                                                       >  1:1903696/1‑149 (MQ=255)
                                                       ggTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAg                                                                                       <  1:2385141/149‑1 (MQ=255)
                                                        gTACAACATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGGTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAgg                                                                                      <  2:2520909/149‑1 (MQ=255)
                                                        gTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCTGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAgg                                                                                      <  2:2151633/149‑1 (MQ=255)
                                                           cAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAggggg                                                                                   >  2:1272257/1‑149 (MQ=255)
                                                              aaTTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCaa                                                                                <  1:1361108/149‑1 (MQ=255)
                                                               aTTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAg                                                                               >  1:1978645/1‑149 (MQ=255)
                                                               aTTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAg                                                                               >  1:2611446/1‑149 (MQ=255)
                                                                      aCTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAAt                                                                        <  1:3803407/149‑1 (MQ=255)
                                                                       cTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATa                                                                       <  2:2512338/149‑1 (MQ=255)
                                                                       cTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATa                                                                       <  2:4033366/149‑1 (MQ=255)
                                                                        tATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAg                                                                      <  1:3452664/149‑1 (MQ=255)
                                                                        tATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAg                                                                      <  2:3091884/149‑1 (MQ=255)
                                                                          tCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCg                                                                    >  2:315231/1‑149 (MQ=255)
                                                                          tCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCg                                                                    >  1:737261/1‑149 (MQ=255)
                                                                           cTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCgg                                                                   >  1:2136505/1‑149 (MQ=255)
                                                                            ttGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGt                                                                  <  2:2897065/149‑1 (MQ=255)
                                                                            ttGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGt                                                                  >  2:896763/1‑149 (MQ=255)
                                                                            ttGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGt                                                                  <  1:1734845/149‑1 (MQ=255)
                                                                                cgccAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCt                                                              >  1:3513556/1‑149 (MQ=255)
                                                                                cgccAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCt                                                              >  1:1227909/1‑149 (MQ=255)
                                                                                 gccAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCtt                                                             >  1:569134/1‑149 (MQ=255)
                                                                                 gccAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCtt                                                             >  1:1419869/1‑149 (MQ=255)
                                                                                  ccAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCttt                                                            <  2:655650/149‑1 (MQ=255)
                                                                                   cAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCtttt                                                           <  2:587854/149‑1 (MQ=255)
                                                                                    aaaGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTc                                                          >  1:3849913/1‑149 (MQ=255)
                                                                                    aaaGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTc                                                          <  1:3818798/149‑1 (MQ=255)
                                                                                      aGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTccc                                                        <  1:2874347/149‑1 (MQ=255)
                                                                                      aGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTccc                                                        <  1:2874148/149‑1 (MQ=255)
                                                                                       gTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCACGCGGTAATAGCGGTAGCTTTTCCCt                                                       >  1:2506284/1‑149 (MQ=255)
                                                                                       gTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCt                                                       <  1:776219/149‑1 (MQ=255)
                                                                                            aTTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGaaaa                                                  <  1:1582199/149‑1 (MQ=255)
                                                                                             ttGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAat                                                 >  2:2748111/1‑149 (MQ=255)
                                                                                             ttGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAat                                                 >  2:2748020/1‑149 (MQ=255)
                                                                                                 aGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTg                                             >  1:3636750/1‑149 (MQ=255)
                                                                                                 aGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTg                                             <  2:806580/149‑1 (MQ=255)
                                                                                                     tAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGc                                         >  2:3690210/1‑149 (MQ=255)
                                                                                                      aaaGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGcc                                        >  1:215041/1‑149 (MQ=255)
                                                                                                        aGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCcgt                                      >  1:3241378/1‑149 (MQ=255)
                                                                                                          gCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCcgttc                                    <  1:1860579/149‑1 (MQ=255)
                                                                                                          gCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCcgttc                                    >  2:1536632/1‑149 (MQ=255)
                                                                                                          gCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCcgttc                                    <  2:2652263/149‑1 (MQ=255)
                                                                                                            aGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCCGGGGGCCAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCcgttcgt                                  >  2:3309046/1‑149 (MQ=255)
                                                                                                            aGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCcgttcgt                                  >  2:767240/1‑149 (MQ=255)
                                                                                                             gTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCcgttcgtt                                 <  2:2613296/149‑1 (MQ=255)
                                                                                                             gTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCcgttcgtt                                 >  2:3004539/1‑149 (MQ=255)
                                                                                                                ctcCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGc                              >  2:2561291/1‑149 (MQ=255)
                                                                                                                 tcCTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCt                             >  1:898328/1‑149 (MQ=255)
                                                                                                                  ccTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTc                            >  2:1092294/1‑149 (MQ=255)
                                                                                                                  ccTACTGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTc                            <  1:2542544/149‑1 (MQ=255)
                                                                                                                       tGCGGTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGAAGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGgtcg                       >  2:1020399/1‑149 (MQ=255)
                                                                                                                          ggTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCa                    >  1:944835/1‑149 (MQ=255)
                                                                                                                          ggTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCa                    >  1:3001986/1‑149 (MQ=255)
                                                                                                                          ggTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCa                    <  2:3751588/149‑1 (MQ=255)
                                                                                                                           gTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCAt                   >  2:2233832/1‑149 (MQ=255)
                                                                                                                           gTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCAt                   >  2:1921117/1‑149 (MQ=255)
                                                                                                                           gTTCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCAt                   >  2:3959312/1‑149 (MQ=255)
                                                                                                                             tCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATtc                 <  2:917757/149‑1 (MQ=255)
                                                                                                                             tCCTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATtc                 >  1:1676495/1‑149 (MQ=255)
                                                                                                                              ccTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAAGAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATtcc                <  1:1452570/149‑1 (MQ=255)
                                                                                                                               cTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATtcct               >  1:1348095/1‑149 (MQ=255)
                                                                                                                               cTTTCGAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATtcct               >  1:3398785/1‑149 (MQ=255)
                                                                                                                                    gAAAACGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTAt          >  2:3333379/1‑149 (MQ=255)
                                                                                                                                     aaaaCGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGNCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTAtg         >  1:2084241/1‑149 (MQ=255)
                                                                                                                                      aaaCGTGGGCACTGGGGGGGATACCCGATGGCATATGTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTAtgg        <  2:3713960/149‑1 (MQ=255)
                                                                                                                                       aaCGTGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTAtggc       >  1:2894807/1‑149 (MQ=255)
                                                                                                                                           tGGGCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATAGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCAAtggcgctg   >  1:2067357/1‑149 (MQ=255)
                                                                                                                                            gggCACTGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTAtggcgctgg  >  1:3228870/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
AGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTATGGCGCTGG  >  CP009273/997527‑997815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑