Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,000,841 G→T 100% A206A (GCG→GCT)  pyrD → dihydro‑orotate oxidase, FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,000,8410GT99.2% 791.7 / ‑1.9 240A206A (GCG→GCT) pyrDdihydro‑orotate oxidase, FMN‑linked
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (122/116);  total (122/118)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 240 total aligned reads displayed.

TATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTC  >  CP009273/1000697‑1000986
                                                                                                                                                |                                                                                                                                                 
tATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGc                                                                                                                                               <  2:1832890/149‑1 (MQ=255)
          aTCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAata                                                                                                                                     <  2:449702/149‑1 (MQ=255)
           tCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAatat                                                                                                                                    >  1:3849628/1‑149 (MQ=255)
            ctatgcctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAtg                                                                                                                                   <  2:2926596/149‑1 (MQ=255)
            ctatgcctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAtg                                                                                                                                   >  2:1146366/1‑149 (MQ=255)
             tatgcctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAtgt                                                                                                                                  <  2:1189742/149‑1 (MQ=255)
             tatgcctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAtgt                                                                                                                                  >  2:3805961/1‑149 (MQ=255)
              atgcctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAtgtg                                                                                                                                 >  1:3708635/1‑149 (MQ=255)
               tgcctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGc                                                                                                                                >  1:3350611/1‑149 (MQ=255)
                gcctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGAGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGcc                                                                                                                               >  2:2678719/1‑149 (MQ=255)
                  ctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGa                                                                                                                             <  1:3111277/149‑1 (MQ=255)
                  ctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGa                                                                                                                             <  1:235205/149‑1 (MQ=255)
                  ctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGa                                                                                                                             <  1:2362946/149‑1 (MQ=255)
                   tatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGAt                                                                                                                            >  2:1594194/1‑149 (MQ=255)
                   tatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGAt                                                                                                                            <  1:2669750/149‑1 (MQ=255)
                    atgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATc                                                                                                                           <  1:1894137/149‑1 (MQ=255)
                      gcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGc                                                                                                                         <  1:557346/149‑1 (MQ=255)
                         ggATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGt                                                                                                                      <  1:26488/149‑1 (MQ=255)
                         ggATATATCGCCAACAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGt                                                                                                                      >  1:1013023/1‑149 (MQ=255)
                           atatatCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGa                                                                                                                    <  1:756918/149‑1 (MQ=255)
                            tatatCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGaa                                                                                                                   <  2:72479/149‑1 (MQ=255)
                              tatCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGa                                                                                                                 <  1:3927805/149‑1 (MQ=255)
                               atCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGAt                                                                                                                <  2:1758220/149‑1 (MQ=255)
                                tCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATc                                                                                                               <  2:363943/149‑1 (MQ=255)
                                tCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATc                                                                                                               <  2:109555/149‑1 (MQ=255)
                                  gCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATcgc                                                                                                             >  2:2388824/1‑149 (MQ=255)
                                     aTCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGcc                                                                                                          <  1:2417710/149‑1 (MQ=255)
                                     aTCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGcc                                                                                                          >  1:3059463/1‑149 (MQ=255)
                                     aTCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGcc                                                                                                          >  1:3059594/1‑149 (MQ=255)
                                     aTCAATATGTCAACGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGcc                                                                                                          <  1:3339263/149‑1 (MQ=255)
                                      tCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCg                                                                                                         >  2:3404592/1‑149 (MQ=255)
                                          tatTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATc                                                                                                     <  1:25803/149‑1 (MQ=255)
                                           atTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCt                                                                                                    <  2:563544/149‑1 (MQ=255)
                                            tttCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCtt                                                                                                   <  2:2476716/149‑1 (MQ=255)
                                            tttCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCtt                                                                                                   <  2:2516123/149‑1 (MQ=255)
                                                     cGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaagaa                                                                                          <  2:130253/149‑1 (MQ=255)
                                                      gAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGACGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaagaag                                                                                         >  1:2542036/1‑149 (MQ=255)
                                                       aaTACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaagaaga                                                                                        >  2:1242102/1‑149 (MQ=255)
                                                       aaTACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaagaaga                                                                                        <  2:2636422/149‑1 (MQ=255)
                                                       aaTACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaagaaga                                                                                        >  1:4083367/1‑149 (MQ=255)
                                                       aaTACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaagaaga                                                                                        >  2:3521889/1‑149 (MQ=255)
                                                        aTACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaagaagaa                                                                                       <  1:357421/149‑1 (MQ=255)
                                                        aTACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaagaagaa                                                                                       >  1:2749287/1‑149 (MQ=255)
                                                        aTACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaagaagaa                                                                                       <  2:2644375/149‑1 (MQ=255)
                                                           ccccAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTg                                                                                    >  2:3250472/1‑149 (MQ=255)
                                                            cccAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGa                                                                                   >  1:2073780/1‑149 (MQ=255)
                                                               aGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATcc                                                                                >  1:1415146/1‑149 (MQ=255)
                                                                ggATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCa                                                                               >  2:2810342/1‑149 (MQ=255)
                                                                ggATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCa                                                                               <  1:2041923/149‑1 (MQ=255)
                                                                 gATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAg                                                                              >  1:239496/1‑149 (MQ=255)
                                                                 gATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAg                                                                              >  1:238519/1‑149 (MQ=255)
                                                                      cgcacgcTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGc                                                                         >  2:1976817/1‑149 (MQ=255)
                                                                              gCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagtt                                                                 <  2:3984060/149‑1 (MQ=255)
                                                                              gCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGATGATCGCGCCGGATCTTTCTGAAGAAGTATTGATCCAGGTTGCCGAtagtt                                                                 <  2:52963/149‑1 (MQ=255)
                                                                               cAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagttt                                                                <  1:1617294/149‑1 (MQ=255)
                                                                                 atatGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagtttag                                                              <  2:1937058/149‑1 (MQ=255)
                                                                                   atGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagtttagtt                                                            <  2:317130/149‑1 (MQ=255)
                                                                                   atGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagtttagtt                                                            <  2:1433159/149‑1 (MQ=255)
                                                                                           gcgcTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCataa                                                    <  2:1687815/149‑1 (MQ=255)
                                                                                             gcTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCataata                                                  >  2:3240911/1‑149 (MQ=255)
                                                                                             gcTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCataata                                                  <  1:2678719/149‑1 (MQ=255)
                                                                                             gcTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCataata                                                  <  2:347186/149‑1 (MQ=255)
                                                                                                  atgatCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGAt                                             <  1:3491734/149‑1 (MQ=255)
                                                                                                   tgatCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATg                                            <  1:1242102/149‑1 (MQ=255)
                                                                                                   tgatCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATg                                            >  1:21017/1‑149 (MQ=255)
                                                                                                      tctcTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCg                                         >  2:1350288/1‑149 (MQ=255)
                                                                                                      tctcTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCg                                         >  2:3399763/1‑149 (MQ=255)
                                                                                                         ctTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTa                                      >  2:3199811/1‑149 (MQ=255)
                                                                                                         ctTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTa                                      <  2:1698490/149‑1 (MQ=255)
                                                                                                          ttAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGATATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTAt                                     <  2:1809863/149‑1 (MQ=255)
                                                                                                            aaCCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTGTAGTTCGCCATAATATTGATGGCGTTATTg                                   >  2:137148/1‑149 (MQ=255)
                                                                                                                    ttAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAAt                           >  1:1541689/1‑149 (MQ=255)
                                                                                                                     tAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATa                          >  1:1979989/1‑149 (MQ=255)
                                                                                                                       aaaaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATAcc                        >  1:1165515/1‑149 (MQ=255)
                                                                                                                       aaaaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATAcc                        >  1:3216528/1‑149 (MQ=255)
                                                                                                                         aaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACcac                      >  1:3141655/1‑149 (MQ=255)
                                                                                                                         aaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACcac                      <  2:3843525/149‑1 (MQ=255)
                                                                                                                           tAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCacac                    <  2:1601397/149‑1 (MQ=255)
                                                                                                                             aGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGTAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCCCCCTc                  >  2:1453157/1‑149 (MQ=255)
                                                                                                                             aGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTc                  >  1:1711582/1‑149 (MQ=255)
                                                                                                                              gCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCg                 >  2:3363312/1‑149 (MQ=255)
                                                                                                                              gCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCg                 >  2:829959/1‑149 (MQ=255)
                                                                                                                                aaaaTGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAATATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGAt               >  2:4069381/1‑149 (MQ=255)
                                                                                                                                 aaaTGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATc              >  1:3893364/1‑149 (MQ=255)
                                                                                                                                     gATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTtc          <  1:2947039/149‑1 (MQ=255)
                                                                                                                                     gATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTtc          >  2:1948369/1‑149 (MQ=255)
                                                                                                                                     gATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTtc          >  2:1282960/1‑149 (MQ=255)
                                                                                                                                     gATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTtc          <  1:888454/149‑1 (MQ=255)
                                                                                                                                        ttGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTctct       <  1:1290713/149‑1 (MQ=255)
                                                                                                                                          gCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTg     >  1:1159119/1‑149 (MQ=255)
                                                                                                                                          gCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTg     <  1:922486/149‑1 (MQ=255)
                                                                                                                                           cAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGt    <  1:3598570/149‑1 (MQ=255)
                                                                                                                                           cAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGt    <  1:277912/149‑1 (MQ=255)
                                                                                                                                           cAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGt    <  2:3103077/149‑1 (MQ=255)
                                                                                                                                            aaGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGtt   <  2:3059463/149‑1 (MQ=255)
                                                                                                                                            aaGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGtt   >  1:1539152/1‑149 (MQ=255)
                                                                                                                                            aaGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGtt   <  2:3059594/149‑1 (MQ=255)
                                                                                                                                             aGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTc  >  2:2183136/1‑149 (MQ=255)
                                                                                                                                             aGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTc  >  1:3739415/1‑149 (MQ=255)
                                                                                                                                             aGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCGTGTTc  >  2:2215725/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTC  >  CP009273/1000697‑1000986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑