Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,024,703 G→T 100% G241G (GGC→GGA)  rlmI ← 23S rRNA m(5)C1962 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,024,7030GT99.3% 963.4 / ‑2.3 294G241G (GGC→GGA) rlmI23S rRNA m(5)C1962 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (130/162);  total (131/163)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 294 total aligned reads displayed.

CGATCGCGATAAGTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCGCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCACCA  >  CP009273/1024557‑1024848
                                                                                                                                                  |                                                                                                                                                 
 gatcgcgatAAGTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTccc                                                                                                                                                <  2:2338125/149‑1 (MQ=255)
    cgcgatAAGTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATc                                                                                                                                             >  2:2572024/1‑149 (MQ=255)
     gcgatAAGTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCa                                                                                                                                            <  2:302020/149‑1 (MQ=255)
      cgatAAGTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAg                                                                                                                                           >  2:2723260/1‑149 (MQ=255)
         tAAGTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGc                                                                                                                                        >  1:485428/1‑149 (MQ=255)
         tAAGTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGc                                                                                                                                        >  2:1908259/1‑149 (MQ=255)
         tAAGTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGc                                                                                                                                        >  1:1616072/1‑149 (MQ=255)
          aaGTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGcc                                                                                                                                       >  2:874449/1‑149 (MQ=255)
               cGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATAc                                                                                                                                  <  1:2759385/149‑1 (MQ=255)
                gcagcaATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACg                                                                                                                                 >  2:1819800/1‑149 (MQ=255)
                   gcaATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCg                                                                                                                              >  2:2985735/1‑149 (MQ=255)
                      aTTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGaaa                                                                                                                           <  2:364502/149‑1 (MQ=255)
                      aTTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGaaa                                                                                                                           >  1:3776438/1‑149 (MQ=255)
                      aTTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGaaa                                                                                                                           <  2:3484018/149‑1 (MQ=255)
                       tttAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAac                                                                                                                          >  2:77807/1‑149 (MQ=255)
                        ttAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAacc                                                                                                                         <  2:1067966/149‑1 (MQ=255)
                         tAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAacca                                                                                                                        <  1:3821065/149‑1 (MQ=255)
                         tAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAacca                                                                                                                        >  1:3672313/1‑149 (MQ=255)
                          aaaGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAaccac                                                                                                                       <  1:2035263/149‑1 (MQ=255)
                          aaaGACATCATCACGGACAAACTCAGCCTTACTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAaccac                                                                                                                       <  1:2008663/149‑1 (MQ=255)
                           aaGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAaccacc                                                                                                                      >  2:2149599/1‑149 (MQ=255)
                             gACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCgg                                                                                                                    >  1:1006171/1‑149 (MQ=255)
                               catcatCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGta                                                                                                                  <  2:3438409/149‑1 (MQ=255)
                                  catcaCGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAg                                                                                                               <  1:3741805/149‑1 (MQ=255)
                                   atcaCGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAgg                                                                                                              >  1:901629/1‑149 (MQ=255)
                                    tcaCGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGga                                                                                                             <  1:2257582/149‑1 (MQ=255)
                                       cGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGaa                                                                                                          >  1:279458/1‑149 (MQ=255)
                                       cGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGaa                                                                                                          >  1:2372587/1‑149 (MQ=255)
                                         gACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAAc                                                                                                        >  2:1727459/1‑149 (MQ=255)
                                         gACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAAc                                                                                                        >  2:3995855/1‑149 (MQ=255)
                                            aaaCTCAACCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGt                                                                                                     <  1:1745915/149‑1 (MQ=255)
                                             aaCTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGtt                                                                                                    <  2:3269485/149‑1 (MQ=255)
                                              aCTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTc                                                                                                   >  1:1090799/1‑149 (MQ=255)
                                              aCTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTc                                                                                                   <  2:565346/149‑1 (MQ=255)
                                              aCTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTc                                                                                                   >  1:1940030/1‑149 (MQ=255)
                                               cTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCa                                                                                                  <  1:3507006/149‑1 (MQ=255)
                                                 cAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGc                                                                                                >  1:1799807/1‑149 (MQ=255)
                                                   gCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGcac                                                                                              >  2:1648527/1‑149 (MQ=255)
                                                   gCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGcac                                                                                              <  2:131608/149‑1 (MQ=255)
                                                    ccTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGcaca                                                                                             <  2:2738592/149‑1 (MQ=255)
                                                     cTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCacac                                                                                            <  2:3404500/149‑1 (MQ=255)
                                                     cTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCacac                                                                                            >  1:3150090/1‑149 (MQ=255)
                                                       tGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGt                                                                                          <  1:1594871/149‑1 (MQ=255)
                                                         cTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGttt                                                                                        >  1:2136335/1‑149 (MQ=255)
                                                         cTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGttt                                                                                        <  2:2886243/149‑1 (MQ=255)
                                                         cTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGttt                                                                                        <  1:2670658/149‑1 (MQ=255)
                                                          tCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTa                                                                                       <  2:1931818/149‑1 (MQ=255)
                                                           cAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTAt                                                                                      <  1:2176497/149‑1 (MQ=255)
                                                           cAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGAACACGTTTAt                                                                                      >  2:3253816/1‑149 (MQ=255)
                                                             gATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTAttt                                                                                    <  1:1649417/149‑1 (MQ=255)
                                                             gATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTAttt                                                                                    >  2:1508630/1‑149 (MQ=255)
                                                              aTCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTAtttt                                                                                   <  1:2808914/149‑1 (MQ=255)
                                                               tCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTc                                                                                  <  2:114617/149‑1 (MQ=255)
                                                                 cAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCaa                                                                                <  2:2221447/149‑1 (MQ=255)
                                                                 cAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCaa                                                                                <  2:124653/149‑1 (MQ=255)
                                                                  aGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAAc                                                                               >  2:831571/1‑149 (MQ=255)
                                                                  aGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAAc                                                                               >  2:831349/1‑149 (MQ=255)
                                                                      tGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAg                                                                           <  1:77807/149‑1 (MQ=255)
                                                                            gCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGcggcgg                                                                     <  1:1903357/149‑1 (MQ=255)
                                                                               cAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTa                                                                  <  2:1586430/149‑1 (MQ=255)
                                                                                      cTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAggc                                                           >  1:1547783/1‑149 (MQ=255)
                                                                                      cTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAggc                                                           <  1:1720481/149‑1 (MQ=255)
                                                                                      cTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAggc                                                           <  1:653345/149‑1 (MQ=255)
                                                                                       tGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAggcg                                                          >  1:1155814/1‑149 (MQ=255)
                                                                                            tGCAATATCCAGCGCGTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTAt                                                     <  2:3961566/149‑1 (MQ=255)
                                                                                             gCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATc                                                    <  2:3007134/149‑1 (MQ=255)
                                                                                              cAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCa                                                   <  2:3676417/149‑1 (MQ=255)
                                                                                               aaTATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCAc                                                  <  2:3361304/149‑1 (MQ=255)
                                                                                               aaTATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCAc                                                  >  1:2628304/1‑149 (MQ=255)
                                                                                                atatCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACg                                                 >  2:1542190/1‑149 (MQ=255)
                                                                                                atatCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACg                                                 >  1:2591920/1‑149 (MQ=255)
                                                                                                 tatCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGc                                                <  2:2998123/149‑1 (MQ=255)
                                                                                                 tatCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGc                                                >  2:245042/1‑149 (MQ=255)
                                                                                                  atCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCt                                               <  2:3215183/149‑1 (MQ=255)
                                                                                                    ccAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTgg                                             <  1:2479540/149‑1 (MQ=255)
                                                                                                     cAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGt                                            <  1:3266259/149‑1 (MQ=255)
                                                                                                      aGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTc                                           <  2:485428/149‑1 (MQ=255)
                                                                                                        cgcTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCa                                         <  2:2179608/149‑1 (MQ=255)
                                                                                                         gcTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGGGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAg                                        <  2:1467786/149‑1 (MQ=255)
                                                                                                          cTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAgg                                       <  2:1046201/149‑1 (MQ=255)
                                                                                                           ttCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGgt                                      <  1:1262021/149‑1 (MQ=255)
                                                                                                            tCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGgta                                     >  1:2284840/1‑149 (MQ=255)
                                                                                                              cTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAgg                                                            >  1:3799653/1‑124 (MQ=255)
                                                                                                              cTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAgg                                                            <  2:3799653/124‑1 (MQ=255)
                                                                                                                gggAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGtagtag                                 >  2:2101950/1‑149 (MQ=255)
                                                                                                                 ggAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGc                                >  1:2436416/1‑149 (MQ=255)
                                                                                                                  gAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGcc                               <  2:2625155/149‑1 (MQ=255)
                                                                                                                         cAACGCTTAAAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGAGAAACCACCGGTATAGGAGAAACAGTACAGCACACGTTTATTTTCAACTTAGCGGCGGGTAGCCAGGCGGCTATCACGCTTGTCCAGGTAGTAGCCCGTTTtg                        <  1:1056360/149‑1 (MQ=255)
                                                                                                                              cTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTcc                   <  2:805869/149‑1 (MQ=255)
                                                                                                                               tGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCg                  <  2:2694662/149‑1 (MQ=255)
                                                                                                                                  cAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGc               <  1:559631/149‑1 (MQ=255)
                                                                                                                                    aCCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTg             >  1:834377/1‑149 (MQ=255)
                                                                                                                                    aCCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTg             <  2:1005267/149‑1 (MQ=255)
                                                                                                                                    aCCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTg             >  1:3286000/1‑149 (MQ=255)
                                                                                                                                       tGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTCGCCCGATTTGTGTCCGTGCTGAat          >  2:2358017/1‑149 (MQ=255)
                                                                                                                                       tGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAat          >  2:1183395/1‑149 (MQ=255)
                                                                                                                                       tGGCTGCAACCGCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAat          <  2:589581/149‑1 (MQ=255)
                                                                                                                                        ggCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAata         <  2:3773329/149‑1 (MQ=255)
                                                                                                                                          cTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATc       <  2:2281011/149‑1 (MQ=255)
                                                                                                                                            gCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATcca     >  1:4105487/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGATCGCGATAAGTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCGCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCACCA  >  CP009273/1024557‑1024848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑