BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,031,861	G→A	15.8%	G18S (GGC→AGC)	hyaE →	putative HyaA chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,031,861	0	G	A	15.8%	592.7 / 82.4	211	G18S (GGC→AGC)	hyaE	putative HyaA chaperone
Reads supporting (aligned to +/- strand): ref base G (101/76); new base A (20/13); total (121/90)
Fisher's exact test for biased strand distribution p-value = 8.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

CTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGA  >  CP009273/1031713‑1032009

  aaTGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGc                                                                                                                                                    <  2:1542828/149‑1 (MQ=255)

  aaTGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGc                                                                                                                                                    <  1:2400540/149‑1 (MQ=255)

                tctcAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCcagtcag                                                                                                                                      >  2:631255/1‑149 (MQ=255)

                    aaTTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGaa                                                                                                                                  >  1:2043118/1‑149 (MQ=255)

                     tttATGACTGTCTGTCGATGGATAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGCGGAGCAGGGATGAGCAACGACACGCCAATTGATGCGTTGTGGCAACGAATGCTGGCGAGCGGCTGGACGCCAGTCAGTGAAt                                                                                                                                 <  2:4098561/148‑1 (MQ=255)

                      ttATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATc                                                                                                                                <  2:3810363/149‑1 (MQ=255)

                      ttATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATc                                                                                                                                >  1:2173103/1‑149 (MQ=255)

                      ttATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGAAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATc                                                                                                                                >  1:781328/1‑149 (MQ=255)

                       tATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATcc                                                                                                                               <  2:750237/149‑1 (MQ=255)

                           aCTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTc                                                                                                                           <  2:2402655/149‑1 (MQ=255)

                             tgtctgtcGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCtt                                                                                                                         >  1:1035130/1‑149 (MQ=255)

                              gtctgtcGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTg                                                                                                                        >  2:3218080/1‑149 (MQ=255)

                              gtctgtcGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTg                                                                                                                        >  2:1105576/1‑149 (MQ=255)

                               tctgtcGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTga                                                                                                                       <  1:1265272/149‑1 (MQ=255)

                                ctgtcGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTgac                                                                                                                      >  2:869309/1‑149 (MQ=255)

                                ctgtcGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTgac                                                                                                                      >  1:321390/1‑149 (MQ=255)

                                  gtcGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTgacga                                                                                                                    >  1:1616278/1‑149 (MQ=255)

                                   tcGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTgacgac                                                                                                                   >  2:1622030/1‑149 (MQ=255)

                                    cGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACt                                                                                                                  <  1:3958093/149‑1 (MQ=255)

                                     gATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTg                                                                                                                 >  1:2844071/1‑149 (MQ=255)

                                     gATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTg                                                                                                                 <  1:3901530/149‑1 (MQ=255)

                                     gATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTg                                                                                                                 >  2:1278048/1‑149 (MQ=255)

                                     gATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTg                                                                                                                 <  1:389472/149‑1 (MQ=255)

                                      aTGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTgg                                                                                                                >  2:1465779/1‑149 (MQ=255)

                                         gAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCtt                                                                                                             <  2:3573026/149‑1 (MQ=255)

                                         gAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCtt                                                                                                             >  2:4080910/1‑149 (MQ=255)

                                         gAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCtt                                                                                                             >  2:3506871/1‑149 (MQ=255)

                                           aaaTTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTAc                                                                                                           <  1:4080910/149‑1 (MQ=255)

                                             aTTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGc                                                                                                         >  1:3084500/1‑149 (MQ=255)

                                               tACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCaa                                                                                                       >  2:676607/1‑149 (MQ=255)

                                                 cGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAgc                                                                                                     >  2:741608/1‑149 (MQ=255)

                                                  gAAGGCGTACGCTTGCGCCAGTAACGGATGACACAGGAGGAGCAGTGAAGAGCAACGACACGCCCTTTGATGAGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCACGTCTTCACGACTGGCTTACGCAAgcg                                                                                                    >  2:212315/1‑149 (MQ=255)

                                                         ttCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACg                                                                                             >  1:3783870/1‑149 (MQ=255)

                                                         ttCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACg                                                                                             >  1:458809/1‑149 (MQ=255)

                                                          tCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACgg                                                                                            <  1:993382/149‑1 (MQ=255)

                                                             cTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCgt                                                                                         >  1:2491848/1‑149 (MQ=255)

                                                                gcgcCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGTTGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCgtggt                                                                                      <  1:2489900/149‑1 (MQ=255)

                                                                    cAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGtta                                                                                  <  1:4102192/149‑1 (MQ=255)

                                                                     aGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGTTGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGttat                                                                                 <  2:1405579/149‑1 (MQ=255)

                                                                     aGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGttat                                                                                 <  2:2194887/149‑1 (MQ=255)

                                                                           ggATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCa                                                                           >  1:1070954/1‑149 (MQ=255)

                                                                           ggATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCa                                                                           <  2:4082440/149‑1 (MQ=255)

                                                                           ggATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCa                                                                           >  1:3124077/1‑149 (MQ=255)

                                                                           ggATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCa                                                                           <  2:2046867/149‑1 (MQ=255)

                                                                           ggATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCa                                                                           <  1:3300862/149‑1 (MQ=255)

                                                                            gATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAg                                                                          >  2:3342176/1‑149 (MQ=255)

                                                                             aTGACTAAGGTGGAGCTGGTATGAGCAACGACACGCAATTTGATGGGTTGTGGAAACGATTGCGGGCGCGCGGCTGCACGCCTGTATGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGt                                                                         <  1:3017439/149‑1 (MQ=255)

                                                                             aTGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGt                                                                         >  1:1309605/1‑149 (MQ=255)

                                                                             aTGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGt                                                                         >  1:4065240/1‑149 (MQ=255)

                                                                              tGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTg                                                                        <  2:3014401/149‑1 (MQ=255)

                                                                               gACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGa                                                                       >  1:3820078/1‑149 (MQ=255)

                                                                                acacAGGTGGAGCCGGTATGAGCAACGACACGCAATTTGTTGCGTTGTGGCAACTAATGCTGGCGCGCAGCTGGACGCCAGTCATTGAATCCCGTCTTGACGACTGGCTTACGCACGCGCCAGACGGCGTGGTGTTATTAAGCAGTGAc                                                                      <  1:1937946/149‑1 (MQ=255)

                                                                                     ggaggaGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGaa                                                                 >  2:3236718/1‑149 (MQ=255)

                                                                                      gaggagCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGaaa                                                                >  1:1320669/1‑149 (MQ=255)

                                                                                         gagCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGATTGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCGTTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAacgc                                                             <  1:1581397/149‑1 (MQ=255)

                                                                                          agCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAacgca                                                            <  2:3998759/149‑1 (MQ=255)

                                                                                           gCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAacgcac                                                           >  2:1585049/1‑149 (MQ=255)

                                                                                             aGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGGTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAacgcacgc                                                         <  1:2498315/149‑1 (MQ=255)

                                                                                             aGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAacgcacgc                                                         >  1:255595/1‑149 (MQ=255)

                                                                                              gggATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGcc                                                        >  2:2099562/1‑149 (MQ=255)

                                                                                                 aTGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCaga                                                     <  2:2305365/149‑1 (MQ=255)

                                                                                                 aTGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCaga                                                     >  2:2584678/1‑149 (MQ=255)

                                                                                                  tGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCagag                                                    <  2:3896545/149‑1 (MQ=255)

                                                                                                     gCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTc                                                 >  2:237411/1‑149 (MQ=255)

                                                                                                       aaCGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAg                                               >  2:2897266/1‑149 (MQ=255)

                                                                                                        aCGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGc                                              >  1:2272461/1‑149 (MQ=255)

                                                                                                           acacGCCAATAGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACTCAAGCGCCAGACGGCGTGGTGTTATTAAGAAGTGACCCGAAACGCACGCCAGAGGTCAGCgct                                           >  2:3083731/1‑147 (MQ=255)

                                                                                                            cacGCCATTTTATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGACTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATa                                          >  1:459965/1‑149 (MQ=255)

                                                                                                            cacGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATa                                          >  1:1116076/1‑149 (MQ=255)

                                                                                                            cacGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATa                                          >  2:2134010/1‑149 (MQ=255)

                                                                                                              cGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAAt                                        <  2:3879611/149‑1 (MQ=255)

                                                                                                                ccATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATcc                                      >  2:544328/1‑149 (MQ=255)

                                                                                                                  aTTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCgg                                    <  1:3433472/149‑1 (MQ=255)

                                                                                                                  aTTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCgg                                    >  2:3390005/1‑149 (MQ=255)

                                                                                                                   tttGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGt                                   <  2:1746904/149‑1 (MQ=255)

                                                                                                                     tGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTaa                                 <  2:1432170/149‑1 (MQ=255)

                                                                                                                        ttcgatgtgGCAACGAATGCTGGCGAGCGGCTGGACGCCATTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAATCGCAAGACGGCGTGGTTTTATTAAGCAGTGACCCGAAACGCACGACAGAGGTCAGCGATAATCCGCTAATGa                              >  2:805111/6‑149 (MQ=255)

                                                                                                                        tGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGa                              >  2:804039/1‑149 (MQ=255)

                                                                                                                        tGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGa                              >  2:3847576/1‑149 (MQ=255)

                                                                                                                        tGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGa                              >  1:1642402/1‑149 (MQ=255)

                                                                                                                         gggTTGTGGCAACGTTTGGTGGCGGGCCGCTGGTTGCAAGTGATTTAATCCGGTCTTGAGGACGGGCTTACGAAAGCGCCTGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGAt                             <  2:1949130/147‑1 (MQ=255)

                                                                                                                         gCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGATGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGAt                             <  2:3648809/149‑1 (MQ=255)

                                                                                                                           gTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTg                           >  2:2841945/1‑149 (MQ=255)

                                                                                                                           gTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTg                           >  2:1677223/1‑149 (MQ=255)

                                                                                                                                 gCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAt                     <  2:3124077/149‑1 (MQ=255)

                                                                                                                                 gCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAt                     <  2:1070954/149‑1 (MQ=255)

                                                                                                                                  cAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAtt                    >  1:2476768/1‑149 (MQ=255)

                                                                                                                                      gAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTg                <  1:2453127/149‑1 (MQ=255)

                                                                                                                                       aaTGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCCGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgc               >  1:700758/1‑149 (MQ=255)

                                                                                                                                       aaTGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgc               <  2:2485636/149‑1 (MQ=255)

                                                                                                                                       aaTGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgc               <  2:519476/149‑1 (MQ=255)

                                                                                                                                       aaTGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgc               <  2:4091937/149‑1 (MQ=255)

                                                                                                                                         tGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgcgc             >  2:2814243/1‑149 (MQ=255)

                                                                                                                                         tGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgcgc             >  2:1035321/1‑149 (MQ=255)

                                                                                                                                            tGGCGCGCAGCTGGACTCCAGTCAGTGATTCCCGTCTTGTCGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCTAAACGCACGCCAGATGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAg          <  2:2542322/149‑1 (MQ=255)

                                                                                                                                                 cgcgGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTcc     <  2:375689/149‑1 (MQ=255)

                                                                                                                                                    ggCTTGACGCCTGTCAGTGAATCCCGTATTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGa  <  1:3216573/149‑1 (MQ=255)

                                                                                                                                                    ggCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGa  <  2:623200/149‑1 (MQ=255)

                                                                                                                                                    ggCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGa  <  1:3217816/149‑1 (MQ=255)

                                                                                                                                                    ggCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGa  >  1:1477824/1‑149 (MQ=255)

CTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGA  >  CP009273/1031713‑1032009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`