Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 97,521 C→A 100% A90A (GCC→GCA)  murC → UDP‑N‑acetylmuramate:L‑alanine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927397,5210CA100.0% 662.6 / ‑6.7 206A90A (GCC→GCA) murCUDP‑N‑acetylmuramate:L‑alanine ligase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (123/82);  total (124/82)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 206 total aligned reads displayed.

GAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCC  >  CP009273/97375‑97665
                                                                                                                                                  |                                                                                                                                                
gAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGa                                                                                                                                                >  1:398518/1‑149 (MQ=255)
gAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGa                                                                                                                                                >  1:1573846/1‑149 (MQ=255)
 aaGGTTATCAGATCAGTGGTCCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGAt                                                                                                                                               <  2:2954119/149‑1 (MQ=255)
   ggTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATaa                                                                                                                                             >  1:3051714/1‑149 (MQ=255)
    gTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAAc                                                                                                                                            >  2:1085419/1‑149 (MQ=255)
    gTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCAAGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATAGCCCGGAAAACGTAAGAGATGCCAGCGTGGTCGGTTTTTCCAGCCCGATTTCTGCAGATAAc                                                                                                                                            >  2:117349/1‑149 (MQ=255)
     ttATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAAcc                                                                                                                                           >  2:333032/1‑149 (MQ=255)
     ttATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAAcc                                                                                                                                           <  2:1814297/149‑1 (MQ=255)
      tATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAAccc                                                                                                                                          >  2:2540868/1‑149 (MQ=255)
       atcagatcagTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCg                                                                                                                                         >  2:2203286/1‑149 (MQ=255)
       atcagatcagTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCg                                                                                                                                         >  2:2498679/1‑149 (MQ=255)
        tcagatcagTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCgg                                                                                                                                        >  2:2680368/1‑149 (MQ=255)
         cagatcagTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGa                                                                                                                                       <  2:1958594/149‑1 (MQ=255)
           gatcagTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGaaa                                                                                                                                     >  1:13647/1‑149 (MQ=255)
           gatcagTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGaaa                                                                                                                                     >  2:3713115/1‑149 (MQ=255)
             tcagTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAAtt                                                                                                                                   >  2:324372/1‑149 (MQ=255)
                gTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTc                                                                                                                                >  1:566878/1‑149 (MQ=255)
                  ggTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTcgc                                                                                                                              >  1:2220724/1‑149 (MQ=255)
                  ggTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTcgc                                                                                                                              <  2:2604194/149‑1 (MQ=255)
                  ggTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTcgc                                                                                                                              <  2:2640095/149‑1 (MQ=255)
                    ttCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTcgccg                                                                                                                            >  1:2008128/1‑149 (MQ=255)
                    ttCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTcgccg                                                                                                                            >  2:3720305/1‑149 (MQ=255)
                        gATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCa                                                                                                                        >  2:2473353/1‑149 (MQ=255)
                        gATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCa                                                                                                                        <  1:258379/149‑1 (MQ=255)
                         aTTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCAt                                                                                                                       >  2:669155/1‑149 (MQ=255)
                         aTTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCAt                                                                                                                       <  2:857529/149‑1 (MQ=255)
                          tttAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATg                                                                                                                      >  1:3375655/1‑149 (MQ=255)
                          tttAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATg                                                                                                                      >  2:3436352/1‑149 (MQ=255)
                                gcCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAgcgc                                                                                                                >  1:1353294/1‑149 (MQ=255)
                                gcCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGGTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAgcgc                                                                                                                >  1:534072/1‑149 (MQ=255)
                                    aaTCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCCTGAAGCGCGTAt                                                                                                            >  1:3955899/1‑149 (MQ=255)
                                    aaTCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTAt                                                                                                            >  2:1539497/1‑149 (MQ=255)
                                        cGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCg                                                                                                        >  2:3828323/1‑149 (MQ=255)
                                          gTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGt                                                                                                      <  1:1663004/149‑1 (MQ=255)
                                          gTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGGACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGt                                                                                                      <  2:1231163/149‑1 (MQ=255)
                                             aCGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGAt                                                                                                   <  1:3346046/149‑1 (MQ=255)
                                                cagcagTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAAACCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGTTCcg                                                                                                >  1:364492/1‑149 (MQ=255)
                                                      ttAATGAATCTCGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGc                                                                                          <  1:3970353/149‑1 (MQ=255)
                                                       tAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGcc                                                                                         >  2:2030503/1‑149 (MQ=255)
                                                            aaTCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAAt                                                                                    <  2:4085406/149‑1 (MQ=255)
                                                             aTCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATg                                                                                   <  2:4032602/149‑1 (MQ=255)
                                                              tCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgc                                                                                  <  1:1153315/149‑1 (MQ=255)
                                                               cTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCTGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgct                                                                                 <  1:978/149‑1 (MQ=255)
                                                               cTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgct                                                                                 <  2:816774/149‑1 (MQ=255)
                                                                tGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgctg                                                                                >  1:936158/1‑149 (MQ=255)
                                                                 gggTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgctgg                                                                               >  1:2921306/1‑149 (MQ=255)
                                                                      cgacgaTTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAg                                                                          <  1:3860133/149‑1 (MQ=255)
                                                                       gacgaTTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGt                                                                         >  1:3707262/1‑149 (MQ=255)
                                                                        acgaTTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGtt                                                                        <  1:333032/149‑1 (MQ=255)
                                                                        acgaTTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGtt                                                                        >  1:2310925/1‑149 (MQ=255)
                                                                           atttatttCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAAACCGGAAATTGTCGCCGCGCATTAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGTGTTAAt                                                                     >  1:718468/1‑149 (MQ=255)
                                                                               atttCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTGGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGGTAATGCGt                                                                 >  2:722478/1‑149 (MQ=255)
                                                                                tttCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCGCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGtt                                                                >  1:3512661/1‑149 (MQ=255)
                                                                                tttCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGtt                                                                >  1:1465027/1‑149 (MQ=255)
                                                                                tttCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGtt                                                                <  2:3436823/149‑1 (MQ=255)
                                                                                  tCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTGTTTTCCAGCGCGATTTCTGCAGATAACCCGGAAAATGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTTAGTTAATGCGtttt                                                              >  1:3065975/1‑149 (MQ=255)
                                                                                    aaCCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCg                                                            >  2:2722672/1‑149 (MQ=255)
                                                                                    aaCCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCg                                                            >  1:2409469/1‑149 (MQ=255)
                                                                                    aaCCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCg                                                            >  1:2381013/1‑149 (MQ=255)
                                                                                     aCCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTGCAGCGCGATTTATGCAGATAAACCCGAAATTTTAGCCGCGCATGAATCGCGTATTACGGTGTTCCGTCGTGCCGAAATGCTGGGGGAGTTAATGCGGTTTCGt                                                           >  1:1773380/1‑149 (MQ=255)
                                                                                     aCCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGt                                                           >  2:3673698/1‑149 (MQ=255)
                                                                                        aTCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCAt                                                        >  2:2699402/1‑149 (MQ=255)
                                                                                         tCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATg                                                       <  2:2862383/149‑1 (MQ=255)
                                                                                         tCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATg                                                       <  2:1188294/149‑1 (MQ=255)
                                                                                            cccGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCa                                                    <  2:1003656/149‑1 (MQ=255)
                                                                                             ccGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCAt                                                   >  2:3568671/1‑149 (MQ=255)
                                                                                                gAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGc                                                >  2:2896204/1‑149 (MQ=255)
                                                                                                 aaaaCGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGcc                                               >  2:1916817/1‑149 (MQ=255)
                                                                                                  aaaCGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAAGTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCa                                              <  2:1573846/149‑1 (MQ=255)
                                                                                                   aaCGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCAt                                             <  2:896835/149‑1 (MQ=255)
                                                                                                   aaCGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAAACCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAAAGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCAt                                             >  1:2540311/1‑149 (MQ=255)
                                                                                                   aaCGTACGTGATGCCAGCGTGGTCGTGGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCAAGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCAt                                             >  1:1494262/1‑149 (MQ=255)
                                                                                                    acgtacgtGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCAtt                                            >  2:2989265/1‑149 (MQ=255)
                                                                                                      gtacgtGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGc                                          >  2:3522180/1‑149 (MQ=255)
                                                                                                      gtacgtGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGc                                          <  2:3445972/149‑1 (MQ=255)
                                                                                                       tacgtGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGcc                                         >  2:211228/1‑149 (MQ=255)
                                                                                                        acgtGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCg                                        >  2:1124812/1‑149 (MQ=255)
                                                                                                           tGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGaa                                     >  2:2754295/1‑149 (MQ=255)
                                                                                                              tGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGc                                  <  2:2141337/149‑1 (MQ=255)
                                                                                                               gCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCa                                 <  2:1771838/149‑1 (MQ=255)
                                                                                                               gCCAGCGTGGTCGTTGTTTCCAGCCCGAATTATGCAGATAAACCCGAAAATGTAGCCGCGCATGAAGCCCGTATTACGGTGATCCGTCGTGCCCAAAATCTGGCTGAGTTAATTAGTTTTCGTCATGGCATCGCCAATTCCGGAACGCa                                 >  1:761477/1‑149 (MQ=255)
                                                                                                                   gCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGc                             >  2:3176689/1‑149 (MQ=255)
                                                                                                                   gCGTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGc                             >  2:1614712/1‑149 (MQ=255)
                                                                                                                     gTGGTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTTCCGGAACGCACGGCaa                           >  1:652891/1‑149 (MQ=255)
                                                                                                                       ggTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATTCTGGCTGAGTTAATTCGTTTTAGTCATGGCATCGCCATTGCCGGAACGCACGGCaaaa                         >  1:1078565/1‑149 (MQ=255)
                                                                                                                       ggTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCaaac                         <  1:1704146/149‑2 (MQ=255)
                                                                                                                       ggTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCaaaa                         >  1:3407438/1‑149 (MQ=255)
                                                                                                                       ggTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCaaaa                         <  2:566868/149‑1 (MQ=255)
                                                                                                                        gTCGTTGTTTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAAc                        >  1:329913/1‑149 (MQ=255)
                                                                                                                         tCGTTGTTTCCAGCGCGATTTCTGCAGATAACACGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACg                       >  2:3281550/1‑149 (MQ=255)
                                                                                                                            ttgttTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACa                    >  2:455521/1‑149 (MQ=255)
                                                                                                                            ttgttTCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACa                    >  1:1523827/1‑149 (MQ=255)
                                                                                                                               tttCCAGCGCGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAacc                 <  1:1858311/149‑1 (MQ=255)
                                                                                                                                     gcgcGATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACcgcg           >  1:2960509/1‑149 (MQ=255)
                                                                                                                                      cgcgATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGa          <  2:329913/149‑1 (MQ=255)
                                                                                                                                      cgcgATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGa          <  2:1998778/149‑1 (MQ=255)
                                                                                                                                         gATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATgg       >  1:3467980/1‑149 (MQ=255)
                                                                                                                                         gATTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATgg       >  1:3101203/1‑149 (MQ=255)
                                                                                                                                          aTTTCTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGt      >  1:2099559/1‑149 (MQ=255)
                                                                                                                                              cTGCAGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTcc  <  1:1332376/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCC  >  CP009273/97375‑97665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑