Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,114,329 C→A 100% L58F (TTG→TTT)  yceJ ← putative cytochrome b561

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,114,3290CA98.5% 675.8 / 0.7 206L58F (TTG→TTT) yceJputative cytochrome b561
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (96/107);  total (97/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 210 total aligned reads displayed.

CTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCA  >  CP009273/1114183‑1114473
                                                                                                                                                  |                                                                                                                                                
cTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAaaaa                                                                                                                                                <  1:2789282/149‑1 (MQ=255)
  gATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAaaaaaa                                                                                                                                              <  1:2562561/149‑1 (MQ=255)
    tGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATa                                                                                                                                            >  2:1691984/1‑149 (MQ=255)
        gATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGa                                                                                                                                        <  1:328985/149‑1 (MQ=255)
         aTGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGAt                                                                                                                                       >  1:2666847/1‑149 (MQ=255)
           ggCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGc                                                                                                                                     >  2:2335433/1‑149 (MQ=255)
            gCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCt                                                                                                                                    >  1:824264/1‑149 (MQ=255)
             cGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCtt                                                                                                                                   <  1:2435506/149‑1 (MQ=255)
              gAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCttt                                                                                                                                  >  2:2082744/1‑149 (MQ=255)
                aaaGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTa                                                                                                                                >  1:3008083/1‑149 (MQ=255)
                 aaGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTAt                                                                                                                               <  2:4007518/149‑1 (MQ=255)
                 aaGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTAt                                                                                                                               >  2:3616668/1‑149 (MQ=255)
                   gCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGc                                                                                                                             <  1:666827/149‑1 (MQ=255)
                   gCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGc                                                                                                                             <  2:3999930/149‑1 (MQ=255)
                      aTAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGt                                                                                                                          <  2:398480/149‑1 (MQ=255)
                      aTAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGt                                                                                                                          <  2:52229/149‑1 (MQ=255)
                       tAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGtt                                                                                                                         <  1:2299731/149‑1 (MQ=255)
                       tAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGtt                                                                                                                         <  2:1252899/149‑1 (MQ=255)
                        aGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTc                                                                                                                        <  1:3888044/149‑1 (MQ=255)
                         ggTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCg                                                                                                                       >  1:3366313/1‑149 (MQ=255)
                          gTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCgg                                                                                                                      <  1:1179240/149‑1 (MQ=255)
                          gTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCgg                                                                                                                      <  2:3756239/149‑1 (MQ=255)
                           tACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCggg                                                                                                                     <  2:1709928/149‑1 (MQ=255)
                           tACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCggg                                                                                                                     <  1:2793691/149‑1 (MQ=255)
                                  cGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTtg                                                                                                              >  1:3310235/1‑149 (MQ=255)
                                   gagaTGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTtgt                                                                                                             <  2:2123424/149‑1 (MQ=255)
                                    agaTGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTtgtg                                                                                                            <  2:2364200/149‑1 (MQ=255)
                                    agaTGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTtgtg                                                                                                            <  2:2302880/149‑1 (MQ=255)
                                     gaTGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGa                                                                                                           >  2:65224/1‑149 (MQ=255)
                                      aTGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGAt                                                                                                          >  2:3142492/1‑149 (MQ=255)
                                        gTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATAc                                                                                                        >  2:1918574/1‑149 (MQ=255)
                                         tCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATAcc                                                                                                       <  2:1163192/149‑1 (MQ=255)
                                           cAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAg                                                                                                     <  1:618906/149‑1 (MQ=255)
                                            aGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGc                                                                                                    <  2:1975364/149‑1 (MQ=255)
                                            aGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGc                                                                                                    <  2:1913513/149‑1 (MQ=255)
                                             gCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGcc                                                                                                   >  2:3198832/1‑149 (MQ=255)
                                                ctTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATc                                                                                                <  2:2707354/149‑1 (MQ=255)
                                                    cGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGtaa                                                                                            <  1:3452002/149‑1 (MQ=255)
                                                    cGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGtaa                                                                                            <  1:130261/149‑1 (MQ=255)
                                                    cGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGtaa                                                                                            <  1:130230/149‑1 (MQ=255)
                                                     ggCAAGACGAGTCATGGGCGAATAACTCGGCAGCTGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGtaat                                                                                           <  2:1113450/149‑1 (MQ=255)
                                                      gCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGtaata                                                                                          >  2:1956259/1‑149 (MQ=255)
                                                      gCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGtaata                                                                                          >  1:398660/1‑149 (MQ=255)
                                                      gCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCCTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGtaata                                                                                          >  1:1949136/1‑149 (MQ=255)
                                                           aCGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGa                                                                                     >  2:2652422/1‑149 (MQ=255)
                                                           aCGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGa                                                                                     <  2:131062/149‑1 (MQ=255)
                                                             gAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGc                                                                                   >  1:270669/1‑149 (MQ=255)
                                                              aGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCg                                                                                  <  1:943659/149‑1 (MQ=255)
                                                               gTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGt                                                                                 <  2:3869446/149‑1 (MQ=255)
                                                               gTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGt                                                                                 >  1:1172980/1‑149 (MQ=255)
                                                                    gggCGAATAACTCGGCAGCGGACTCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCa                                                                            <  2:345761/149‑1 (MQ=255)
                                                                    gggCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCa                                                                            >  1:1015463/1‑149 (MQ=255)
                                                                         aaTAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCcac                                                                       >  1:3490198/1‑149 (MQ=255)
                                                                         aaTAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCcac                                                                       >  2:3457140/1‑149 (MQ=255)
                                                                         aaTAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCcac                                                                       >  2:482607/1‑149 (MQ=255)
                                                                            aaCTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGa                                                                    <  1:3616668/149‑1 (MQ=255)
                                                                               tCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCt                                                                 >  2:1097692/1‑149 (MQ=255)
                                                                                cGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTa                                                                >  2:3005993/1‑149 (MQ=255)
                                                                                 ggCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTaa                                                               <  1:2108837/149‑1 (MQ=255)
                                                                                  gCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTaaa                                                              <  2:56303/149‑1 (MQ=255)
                                                                                    aGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGc                                                            >  1:815263/1‑149 (MQ=255)
                                                                                      cGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCaa                                                          <  1:1240051/149‑1 (MQ=255)
                                                                                      cGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCaa                                                          <  1:4036905/149‑1 (MQ=255)
                                                                                        gACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAAc                                                        >  2:3325549/1‑149 (MQ=255)
                                                                                        gACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATAACAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAAc                                                        >  1:3179347/1‑149 (MQ=255)
                                                                                        gACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCATTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCAACCACAGACCAAAAGCAAAc                                                        >  1:485870/1‑149 (MQ=255)
                                                                                            cggcggTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGc                                                    <  1:1578138/149‑1 (MQ=255)
                                                                                             ggcggTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGcc                                                   <  2:3366313/149‑1 (MQ=255)
                                                                                             ggcggTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGcc                                                   >  1:2279163/1‑149 (MQ=255)
                                                                                             ggcggTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGcc                                                   <  2:3667308/149‑1 (MQ=255)
                                                                                              gcggTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCg                                                  <  1:3940115/149‑1 (MQ=255)
                                                                                              gcggTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCg                                                  >  1:964401/1‑149 (MQ=255)
                                                                                               cggTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGt                                                 <  1:3516836/149‑1 (MQ=255)
                                                                                                 gTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAg                                               <  1:1261237/149‑1 (MQ=255)
                                                                                                 gTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAg                                               >  2:797686/1‑149 (MQ=255)
                                                                                                 gTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAg                                               >  1:3657802/1‑149 (MQ=255)
                                                                                                      gaTATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACaat                                          <  2:2708173/149‑1 (MQ=255)
                                                                                                        tatGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACaataa                                        <  2:1015463/149‑1 (MQ=255)
                                                                                                        tatGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACaataa                                        >  2:1461448/1‑149 (MQ=255)
                                                                                                                gCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCtt                                >  2:1742123/1‑149 (MQ=255)
                                                                                                                  cacaGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAg                              >  2:2953949/1‑149 (MQ=255)
                                                                                                                    caGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGcc                            >  2:2617236/1‑149 (MQ=255)
                                                                                                                      gAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCaa                          >  1:2851026/1‑149 (MQ=255)
                                                                                                                      gAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCaa                          >  1:3520507/1‑149 (MQ=255)
                                                                                                                            gAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAg                    <  1:1840085/149‑1 (MQ=255)
                                                                                                                             aaTAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAgg                   >  1:3574676/1‑149 (MQ=255)
                                                                                                                             aaTAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAAGGGACgg                   >  2:2288207/1‑149 (MQ=255)
                                                                                                                              aTAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGc                  <  1:2335433/149‑1 (MQ=255)
                                                                                                                              aTAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGc                  <  1:2219421/149‑1 (MQ=255)
                                                                                                                              aTAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGc                  <  2:2553500/149‑1 (MQ=255)
                                                                                                                                  ccAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCa              <  1:777110/149‑1 (MQ=255)
                                                                                                                                  ccAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCa              <  1:1494298/149‑1 (MQ=255)
                                                                                                                                  ccAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCa              >  1:143/1‑149 (MQ=255)
                                                                                                                                   cAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAg             >  1:989588/1‑149 (MQ=255)
                                                                                                                                    aGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGa            <  1:3754767/149‑1 (MQ=255)
                                                                                                                                    aGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGa            <  1:3650145/149‑1 (MQ=255)
                                                                                                                                    aGCCCCATCATTAAAAAAATACCGATGCTTTTATGCACTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGa            <  2:3250175/149‑1 (MQ=255)
                                                                                                                                      ccccATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGata          <  1:3198886/149‑1 (MQ=255)
                                                                                                                                         catcatTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATaa       <  2:3777593/149‑1 (MQ=255)
                                                                                                                                           tcatTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAaca     >  1:2034829/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCA  >  CP009273/1114183‑1114473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑