Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,122,429 G→T 19.4% S56S (TCG→TCT)  yceM → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,122,4290GT19.4% 630.0 / 93.9 229S56S (TCG→TCT) yceMputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (79/105);  new base T (25/20);  total (104/125)
Fisher's exact test for biased strand distribution p-value = 1.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 229 total aligned reads displayed.

CGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAAC  >  CP009273/1122282‑1122574
                                                                                                                                                   |                                                                                                                                                 
cGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGt                                                                                                                                                  <  2:2431052/149‑1 (MQ=255)
  tAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTa                                                                                                                                                <  1:1081687/149‑1 (MQ=255)
  tAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTa                                                                                                                                                <  1:2320520/149‑1 (MQ=255)
   aGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTAt                                                                                                                                               >  2:2097682/1‑149 (MQ=255)
   aGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTAt                                                                                                                                               <  1:2674745/149‑1 (MQ=255)
    gTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATc                                                                                                                                              <  1:2011707/149‑1 (MQ=255)
    gTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATc                                                                                                                                              <  1:2821168/149‑1 (MQ=255)
    gTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATc                                                                                                                                              >  1:2450361/1‑149 (MQ=255)
    gTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATc                                                                                                                                              >  1:2241743/1‑149 (MQ=255)
    gTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATc                                                                                                                                              <  1:2027973/149‑1 (MQ=255)
      gggATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCa                                                                                                                                            >  1:3998365/1‑149 (MQ=255)
         aTTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGcc                                                                                                                                         >  1:3528903/1‑149 (MQ=255)
          ttAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCt                                                                                                                                        <  1:1696899/149‑1 (MQ=255)
              gTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTgcc                                                                                                                                    <  2:590790/149‑1 (MQ=255)
               tGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTgccg                                                                                                                                   >  2:3485589/1‑149 (MQ=255)
                 gCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTgccgcc                                                                                                                                 >  2:3620595/1‑149 (MQ=255)
                  cATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCa                                                                                                                                <  2:823867/149‑1 (MQ=255)
                   aTTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAg                                                                                                                               >  1:2074899/1‑149 (MQ=255)
                    ttGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGt                                                                                                                              <  2:2301991/149‑1 (MQ=255)
                    ttGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGt                                                                                                                              <  1:59622/149‑1 (MQ=255)
                         cAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGa                                                                                                                         <  2:2717609/149‑1 (MQ=255)
                          aaaaaGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTAGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGAt                                                                                                                        >  2:2055596/1‑149 (MQ=255)
                                cGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGtt                                                                                                                  <  2:2685822/149‑1 (MQ=255)
                                 gTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGttt                                                                                                                 >  1:3572277/1‑149 (MQ=255)
                                  tGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGtttt                                                                                                                <  2:3192186/149‑1 (MQ=255)
                                     ttACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTtgt                                                                                                             <  1:3807771/149‑1 (MQ=255)
                                     ttACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTtgt                                                                                                             >  2:1532879/1‑149 (MQ=255)
                                      tACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTtgtg                                                                                                            >  2:4082201/1‑149 (MQ=255)
                                          ggTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCAtt                                                                                                        <  2:227433/149‑1 (MQ=255)
                                            tGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTcc                                                                                                      <  1:31199/149‑1 (MQ=255)
                                            tGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTcc                                                                                                      <  1:30313/149‑1 (MQ=255)
                                            tGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTcc                                                                                                      <  1:1836937/149‑1 (MQ=255)
                                            tGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTcc                                                                                                      >  2:235993/1‑149 (MQ=255)
                                                 gcggcAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCAc                                                                                                 <  1:2258107/149‑1 (MQ=255)
                                                    gcAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCgc                                                                                              >  2:960063/1‑149 (MQ=255)
                                                    gcAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCgc                                                                                              >  1:2731175/1‑149 (MQ=255)
                                                      aGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCgcca                                                                                            >  2:3949295/1‑149 (MQ=255)
                                                       gCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCgccag                                                                                           >  1:542317/1‑149 (MQ=255)
                                                          tCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGAGGTTTTTGTGCATTCCAGAACCgccagcca                                                                                        >  2:494698/1‑149 (MQ=255)
                                                           cTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCAc                                                                                       <  2:3297382/149‑1 (MQ=255)
                                                            tGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACt                                                                                      <  2:294095/149‑1 (MQ=255)
                                                             gACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACtt                                                                                     <  2:3109777/149‑1 (MQ=255)
                                                              aCTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACttt                                                                                    >  2:1464415/1‑149 (MQ=255)
                                                                 ggACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGAc                                                                                 >  2:186136/1‑149 (MQ=255)
                                                                 ggACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGAc                                                                                 >  2:1271391/1‑149 (MQ=255)
                                                                   aCGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGt                                                                               >  1:1516973/1‑149 (MQ=255)
                                                                     gTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTgg                                                                             >  2:2376247/1‑149 (MQ=255)
                                                                      ttACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATTCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGt                                                                            >  1:2996647/1‑149 (MQ=255)
                                                                       tACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTc                                                                           <  1:2536251/149‑1 (MQ=255)
                                                                        aCAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCa                                                                          <  1:44246/149‑1 (MQ=255)
                                                                         cAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAg                                                                         <  2:282105/149‑1 (MQ=255)
                                                                           aGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTa                                                                       >  2:803992/1‑149 (MQ=255)
                                                                           aGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTa                                                                       >  1:1191905/1‑149 (MQ=255)
                                                                           aGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTa                                                                       >  2:1929091/1‑149 (MQ=255)
                                                                           aGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTa                                                                       <  2:1108262/149‑1 (MQ=255)
                                                                            ggAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTAc                                                                      >  2:1524748/1‑149 (MQ=255)
                                                                             gAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACg                                                                     >  2:888311/1‑149 (MQ=255)
                                                                                ccTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTa                                                                  <  1:1485169/149‑1 (MQ=255)
                                                                                 cTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTAc                                                                 <  2:1969430/149‑1 (MQ=255)
                                                                                 cTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTAc                                                                 <  2:3528903/149‑1 (MQ=255)
                                                                                   ngTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTc                                                               >  1:70243/2‑149 (MQ=255)
                                                                                    gTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCa                                                              >  1:100198/1‑149 (MQ=255)
                                                                                    gTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCa                                                              >  1:99902/1‑149 (MQ=255)
                                                                                    gTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCa                                                              >  2:1534572/1‑149 (MQ=255)
                                                                                     tCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCaa                                                             <  1:899143/149‑1 (MQ=255)
                                                                                      cGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAAt                                                            <  2:2323305/149‑1 (MQ=255)
                                                                                      cGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAAt                                                            <  1:2574947/149‑1 (MQ=255)
                                                                                      cGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAAt                                                            <  2:2539063/149‑1 (MQ=255)
                                                                                       gCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATg                                                           >  2:1190918/1‑149 (MQ=255)
                                                                                        ccTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGc                                                          <  1:1205243/149‑1 (MQ=255)
                                                                                           aCGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCggg                                                       >  2:2116553/1‑149 (MQ=255)
                                                                                           aCGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCggg                                                       >  2:2115881/1‑149 (MQ=255)
                                                                                            cgcgcgcgAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCgggg                                                      <  1:190868/149‑1 (MQ=255)
                                                                                             gcgcgcgAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCggggg                                                     <  2:3833952/149‑1 (MQ=255)
                                                                                              cgcgcgAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGgtgt                                                    <  2:2794052/149‑1 (MQ=255)
                                                                                                 gcgAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACa                                                 <  2:3999362/149‑1 (MQ=255)
                                                                                                  cgAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACAt                                                <  1:778280/149‑1 (MQ=255)
                                                                                                    aaaGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGt                                              <  2:1894058/149‑1 (MQ=255)
                                                                                                       gCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCtg                                           <  2:3022964/149‑1 (MQ=255)
                                                                                                       gCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCGTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCtg                                           <  2:3257953/149‑1 (MQ=255)
                                                                                                          cTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTgtgt                                        >  1:3371998/1‑149 (MQ=255)
                                                                                                           tGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTc                                       <  1:1644321/149‑1 (MQ=255)
                                                                                                               aaTTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATa                                   >  2:2343131/1‑149 (MQ=255)
                                                                                                                  ttGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAAc                                <  2:1754815/149‑1 (MQ=255)
                                                                                                                  ttGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCNTTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAAc                                <  1:2085137/149‑1 (MQ=255)
                                                                                                                      gAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCt                            <  2:1800539/149‑1 (MQ=255)
                                                                                                                      gAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCt                            >  1:2752671/1‑149 (MQ=255)
                                                                                                                      gAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCt                            >  1:3468734/1‑149 (MQ=255)
                                                                                                                          gCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCa                        >  1:802108/1‑149 (MQ=255)
                                                                                                                                 cATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATc                 >  1:1275099/1‑149 (MQ=255)
                                                                                                                                    tCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTgc              <  2:3771940/149‑1 (MQ=255)
                                                                                                                                      cTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTgcgc            >  1:4019511/1‑149 (MQ=255)
                                                                                                                                        tATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGa          >  2:2091923/1‑149 (MQ=255)
                                                                                                                                         aTGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGAt         >  2:657432/1‑149 (MQ=255)
                                                                                                                                         aTGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGAt         <  2:489563/149‑1 (MQ=255)
                                                                                                                                         aTGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGAt         <  2:3984865/149‑1 (MQ=255)
                                                                                                                                           gCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGc       <  1:3627344/149‑1 (MQ=255)
                                                                                                                                             cGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTg     <  2:1201619/149‑1 (MQ=255)
                                                                                                                                               aTTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGaa   <  2:369939/149‑1 (MQ=255)
                                                                                                                                                ttCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAAc  >  2:897169/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAAC  >  CP009273/1122282‑1122574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑