Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,122,994 C→A 100% R245S (CGC→AGC)  yceM → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,122,9940CA95.3% 572.6 / 18.0 192R245S (CGC→AGC) yceMputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (8/1);  new base A (85/98);  total (93/99)
Fisher's exact test for biased strand distribution p-value = 1.58e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 194 total aligned reads displayed.

GTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGC  >  CP009273/1122849‑1123139
                                                                                                                                                 |                                                                                                                                                 
gTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGagcg                                                                                                                                                <  2:705126/149‑1 (MQ=255)
 tttGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGAGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCgg                                                                                                                                               >  1:2180361/1‑149 (MQ=255)
   tGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGc                                                                                                                                             >  1:1018007/1‑149 (MQ=255)
   tGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGc                                                                                                                                             <  2:3091175/149‑1 (MQ=255)
    gCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCa                                                                                                                                            >  2:3623514/1‑149 (MQ=255)
     ccGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGTGTGAGGAGAGCGGGCAg                                                                                                                                           <  2:645180/149‑1 (MQ=255)
     ccGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAg                                                                                                                                           <  1:3193426/149‑1 (MQ=255)
     ccGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAg                                                                                                                                           <  1:2372713/149‑1 (MQ=255)
        aGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGc                                                                                                                                        >  1:2708945/1‑149 (MQ=255)
        aGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGc                                                                                                                                        >  1:2512811/1‑149 (MQ=255)
         gCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCg                                                                                                                                       >  1:870711/1‑149 (MQ=255)
           aCCAGTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTa                                                                                                                                     <  2:2992991/149‑1 (MQ=255)
             cATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGt                                                                                                                                   <  2:3014434/149‑1 (MQ=255)
             cATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGt                                                                                                                                   <  2:3742298/149‑1 (MQ=255)
              aTTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTg                                                                                                                                  >  2:2158642/1‑149 (MQ=255)
              aTTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTg                                                                                                                                  >  2:79133/1‑149 (MQ=255)
              aTTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTg                                                                                                                                  >  1:446325/1‑149 (MQ=255)
               tttttCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGc                                                                                                                                 <  1:231520/149‑1 (MQ=255)
               tttttCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGc                                                                                                                                 <  1:231813/149‑1 (MQ=255)
                 tttCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCAt                                                                                                                               >  1:2160987/1‑149 (MQ=255)
                  ttCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATa                                                                                                                              <  1:1218444/149‑1 (MQ=255)
                  ttCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATa                                                                                                                              >  2:2131029/1‑149 (MQ=255)
                   tCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATaa                                                                                                                             <  1:118541/149‑1 (MQ=255)
                    cGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATaaa                                                                                                                            <  2:3336934/149‑1 (MQ=255)
                      gCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAAcc                                                                                                                          <  2:1955056/149‑1 (MQ=255)
                        tGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGa                                                                                                                        >  1:3058947/1‑149 (MQ=255)
                         ggTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGAt                                                                                                                       >  1:631074/1‑149 (MQ=255)
                          gTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGAtt                                                                                                                      <  1:3262512/149‑1 (MQ=255)
                           tCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTc                                                                                                                     >  2:882321/1‑149 (MQ=255)
                           tCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTc                                                                                                                     >  1:2251202/1‑149 (MQ=255)
                            ccTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTcc                                                                                                                    >  2:2595008/1‑149 (MQ=255)
                                tGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGt                                                                                                                <  1:1800080/149‑1 (MQ=255)
                                tGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGt                                                                                                                <  1:2325763/149‑1 (MQ=255)
                                   aGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTgg                                                                                                             >  1:2767298/1‑149 (MQ=255)
                                     aTCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCa                                                                                                           >  2:2259559/1‑149 (MQ=255)
                                           accTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTAc                                                                                                     <  2:105945/149‑1 (MQ=255)
                                            ccTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACg                                                                                                    >  2:3802176/1‑149 (MQ=255)
                                            ccTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACg                                                                                                    >  2:1561846/1‑149 (MQ=255)
                                             cTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGc                                                                                                   >  2:2060415/1‑149 (MQ=255)
                                                tATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTg                                                                                                <  1:3534089/149‑1 (MQ=255)
                                                tATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTg                                                                                                >  2:2700654/1‑149 (MQ=255)
                                                 aTGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGa                                                                                               <  2:817749/149‑1 (MQ=255)
                                                  tGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAg                                                                                              <  1:3733907/149‑1 (MQ=255)
                                                      tCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAAc                                                                                          >  2:1149390/1‑149 (MQ=255)
                                                        gCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGt                                                                                        >  2:992997/1‑149 (MQ=255)
                                                        gCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGt                                                                                        <  1:2979224/149‑1 (MQ=255)
                                                         ccGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTg                                                                                       <  1:2333811/149‑1 (MQ=255)
                                                          cGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTgg                                                                                      <  2:2186279/149‑1 (MQ=255)
                                                            tGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGt                                                                                    <  2:2916035/149‑1 (MQ=255)
                                                             gCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGtt                                                                                   <  2:353921/149‑1 (MQ=255)
                                                               cGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTg                                                                                 <  2:786945/149‑1 (MQ=255)
                                                                 gAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTc                                                                               <  1:864638/149‑1 (MQ=255)
                                                                 gAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTc                                                                               <  2:1367728/149‑1 (MQ=255)
                                                                 gAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTc                                                                               <  1:922238/149‑1 (MQ=255)
                                                                    gTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGc                                                                            <  1:1416662/149‑1 (MQ=255)
                                                                      cAGCGTGACACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCtg                                                                          <  1:3832789/149‑1 (MQ=255)
                                                                      cAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCtg                                                                          >  2:1454355/1‑149 (MQ=255)
                                                                      cAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCtg                                                                          <  2:2921477/149‑1 (MQ=255)
                                                                       aGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCtgt                                                                         >  1:1467653/1‑149 (MQ=255)
                                                                        gCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCtgtg                                                                        >  2:3147973/1‑149 (MQ=255)
                                                                          gttaaaCCGTGCATGCAGTGACTGACGCTGAGAACATCGACATTACGGATATGAGCGAATGTCGTGAGGAGAGCTTGCAGGGATTAGTGCATAAACCGATTCCTGGTTGTCAGAGTAAGCTAGAGCAACGAGGGAATGTCGTATGTgcg                                                                      >  2:922830/4‑149 (MQ=255)
                                                                            gAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGcgcg                                                                    <  1:2069472/149‑1 (MQ=255)
                                                                             aaaCCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCgg                                                                   >  2:773643/1‑149 (MQ=255)
                                                                             aaaCCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCgg                                                                   >  1:2596845/1‑149 (MQ=255)
                                                                               aCCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCa                                                                 >  1:1013876/1‑149 (MQ=255)
                                                                                 cGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACt                                                               <  1:1049364/149‑1 (MQ=255)
                                                                                  gTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACtt                                                              <  2:783295/149‑1 (MQ=255)
                                                                                    gCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCa                                                            >  2:1391605/1‑149 (MQ=255)
                                                                                       ggCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGAGCGGCACTTCATTg                                                         >  2:1525731/1‑149 (MQ=255)
                                                                                           gTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAAtg                                                     >  1:2452754/1‑149 (MQ=255)
                                                                                           gTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAAtg                                                     >  1:3473816/1‑149 (MQ=255)
                                                                                             gactgacGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAAtgtg                                                   <  2:646507/149‑1 (MQ=255)
                                                                                              actgacGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAAtgtgt                                                  <  1:332709/149‑1 (MQ=255)
                                                                                              actgacGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAAtgtgt                                                  <  1:3962596/149‑1 (MQ=255)
                                                                                              actgacGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAAtgtgt                                                  >  2:2044994/1‑149 (MQ=255)
                                                                                               ctgacGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAAtgtgtg                                                 >  2:3878038/1‑149 (MQ=255)
                                                                                                     gTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAAc                                           >  2:2916270/1‑149 (MQ=255)
                                                                                                     gTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAAc                                           >  2:1975228/1‑149 (MQ=255)
                                                                                                          cTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGAc                                      >  2:2315688/1‑149 (MQ=255)
                                                                                                          cTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGAc                                      <  2:121836/149‑1 (MQ=255)
                                                                                                           tCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACa                                     >  2:2816220/1‑149 (MQ=255)
                                                                                                            cATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAg                                    <  1:372919/149‑1 (MQ=255)
                                                                                                              tCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGtt                                  <  2:423713/149‑1 (MQ=255)
                                                                                                                   aTTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCa                             >  1:3328943/1‑149 (MQ=255)
                                                                                                                    ttACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCaa                            >  1:2472036/1‑149 (MQ=255)
                                                                                                                      aCGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAAc                          <  1:3370679/149‑1 (MQ=255)
                                                                                                                           tatGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAAccgccg                     >  1:1665086/1‑149 (MQ=255)
                                                                                                                            atGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCgg                    <  2:3371937/149‑1 (MQ=255)
                                                                                                                            atGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCgg                    >  1:783868/1‑149 (MQ=255)
                                                                                                                            atGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCgg                    >  1:291774/1‑149 (MQ=255)
                                                                                                                             tGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGc                   >  1:814176/1‑149 (MQ=255)
                                                                                                                                   aaTGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAg             >  1:3244790/1‑149 (MQ=255)
                                                                                                                                   aaTGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAg             >  1:42481/1‑149 (MQ=255)
                                                                                                                                    aTGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAgg            <  2:458335/149‑1 (MQ=255)
                                                                                                                                    aTGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAgg            <  1:187680/149‑1 (MQ=255)
                                                                                                                                    aTGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAgg            >  2:2775644/1‑149 (MQ=255)
                                                                                                                                     tGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGc           <  2:2238047/149‑1 (MQ=255)
                                                                                                                                           gaggagAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCt     <  1:1242518/149‑1 (MQ=255)
                                                                                                                                           gaggagAGCGGGCAGGGCGGAGTGCATAAACCGAGTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACGTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCt     <  2:695068/149‑1 (MQ=255)
                                                                                                                                             ggagAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTgg   <  1:119064/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGC  >  CP009273/1122849‑1123139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑