Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,139,402 C→A 100% R141L (CGC→CTC)  rne ← fused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,139,4020CA97.7% 695.0 / 5.8 220R141L (CGC→CTC) rnefused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base A (99/116);  total (102/118)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 227 total aligned reads displayed.

GTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAAC  >  CP009273/1139256‑1139545
                                                                                                                                                  |                                                                                                                                               
gtTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGgaga                                                                                                                                               >  1:1221948/1‑149 (MQ=255)
 tttCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGagag                                                                                                                                              >  1:3596114/1‑149 (MQ=255)
     aGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAAt                                                                                                                                          <  1:3663179/149‑1 (MQ=255)
        cGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGcc                                                                                                                                       <  2:744688/149‑1 (MQ=255)
          gAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCAc                                                                                                                                     >  2:481673/1‑149 (MQ=255)
           aaGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTACTTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCAcc                                                                                                                                    <  2:3758561/149‑1 (MQ=255)
            aGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGTCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCAccc                                                                                                                                   <  2:2719731/149‑1 (MQ=255)
            aGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCAccc                                                                                                                                   <  2:3631652/149‑1 (MQ=255)
            aGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCAccc                                                                                                                                   >  2:1306048/1‑149 (MQ=255)
                  aaTCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGACATGCCACCcgcgcgc                                                                                                                             >  2:2956372/1‑149 (MQ=255)
                  aaTCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCcgcgcgc                                                                                                                             >  2:139008/1‑149 (MQ=255)
                  aaTCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCcgcgcgc                                                                                                                             >  2:3013996/1‑149 (MQ=255)
                   aTCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcgcgcg                                                                                                                            >  1:2763714/1‑149 (MQ=255)
                      ccATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGgt                                                                                                                         <  2:3988462/149‑1 (MQ=255)
                         ttGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGgttgt                                                                                                                      <  2:1023943/149‑1 (MQ=255)
                         ttGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGgttgt                                                                                                                      >  1:1919673/1‑149 (MQ=255)
                         ttGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGgttgt                                                                                                                      <  2:1542814/149‑1 (MQ=255)
                           gCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTc                                                                                                                    <  2:1705975/149‑1 (MQ=255)
                            cAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCg                                                                                                                   <  1:3144290/149‑1 (MQ=255)
                                  cTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCa                                                                                                             >  1:3046733/1‑149 (MQ=255)
                                   tCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAg                                                                                                            >  2:584146/1‑149 (MQ=255)
                                    cagcagATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGa                                                                                                           <  2:3006697/149‑1 (MQ=255)
                                     agcagATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGaa                                                                                                          >  2:3891129/1‑149 (MQ=255)
                                     agcagATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGaa                                                                                                          <  1:3170395/149‑1 (MQ=255)
                                      gcagATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAAc                                                                                                         >  1:2341814/1‑149 (MQ=255)
                                        ngATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCa                                                                                                       >  1:1424008/2‑149 (MQ=255)
                                        agATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCa                                                                                                       >  1:3472633/1‑149 (MQ=255)
                                              gCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCTCCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGc                                                                                                 <  1:3420663/149‑1 (MQ=255)
                                                 gACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTAc                                                                                              >  2:2677391/1‑149 (MQ=255)
                                                 gACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTAc                                                                                              >  2:1693480/1‑149 (MQ=255)
                                                     ccAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGc                                                                                          >  1:1973371/1‑149 (MQ=255)
                                                         cGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGa                                                                                      >  2:2637127/1‑149 (MQ=255)
                                                              cgcACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGAt                                                                                 >  2:77815/1‑149 (MQ=255)
                                                                   gATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAgg                                                                            <  1:1547974/149‑1 (MQ=255)
                                                                     tAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAggtg                                                                          >  2:2558062/1‑149 (MQ=255)
                                                                      aaGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAggtgg                                                                         <  1:1155748/149‑1 (MQ=255)
                                                                            cATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATg                                                                   <  1:1906005/149‑1 (MQ=255)
                                                                            cATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATg                                                                   <  1:651980/149‑1 (MQ=255)
                                                                             aTGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGc                                                                  >  1:1260711/1‑149 (MQ=255)
                                                                             aTGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGc                                                                  >  1:1019980/1‑149 (MQ=255)
                                                                                    ccGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCt                                                           <  1:2891186/149‑1 (MQ=255)
                                                                                      ggCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCttt                                                         >  2:2666169/1‑149 (MQ=255)
                                                                                       gCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCNCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTttg                                                        >  1:2085195/1‑149 (MQ=255)
                                                                                       gCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTttg                                                        <  1:461578/149‑1 (MQ=255)
                                                                                         aGTTCAAGGCTTGCCAGTTCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTttgtt                                                      <  2:3930260/149‑1 (MQ=255)
                                                                                         aGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTttgtt                                                      <  1:1177075/149‑1 (MQ=255)
                                                                                          gTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTttgttg                                                     >  2:931805/1‑149 (MQ=255)
                                                                                          gTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTttgttg                                                     >  2:861512/1‑149 (MQ=255)
                                                                                          gTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAAGGCCGCGCCTtggttg                                                     >  2:2606076/1‑149 (MQ=255)
                                                                                            tCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGcc                                                   >  1:1848710/1‑149 (MQ=255)
                                                                                             cAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCcg                                                  >  1:1702729/1‑149 (MQ=255)
                                                                                               aGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCcgcg                                                >  1:2207715/1‑149 (MQ=255)
                                                                                                ggCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCgcgc                                               <  1:139008/149‑1 (MQ=255)
                                                                                                  cTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGctc                                             <  2:1237518/149‑1 (MQ=255)
                                                                                                   ttGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGACCCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGctct                                            <  2:1649488/149‑1 (MQ=255)
                                                                                                    tGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtctt                                           >  2:4060109/1‑149 (MQ=255)
                                                                                                     gCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtcttc                                          >  2:669888/1‑149 (MQ=255)
                                                                                                      ccAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtcttct                                         <  1:861512/149‑1 (MQ=255)
                                                                                                      ccAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtcttct                                         <  1:334552/149‑1 (MQ=255)
                                                                                                       cAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTcttctt                                        <  2:434686/149‑1 (MQ=255)
                                                                                                       cAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTcttctt                                        <  1:2233715/149‑1 (MQ=255)
                                                                                                       cAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTcttctt                                        >  1:3262624/1‑149 (MQ=255)
                                                                                                       cAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCGTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTcttctt                                        <  2:3263304/149‑1 (MQ=255)
                                                                                                        aGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCttt                                       >  2:2338699/1‑149 (MQ=255)
                                                                                                        aGTGCTTCTTTTAATTCGGTACGGTAGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCttt                                       >  2:3414052/1‑149 (MQ=255)
                                                                                                         gTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTa                                      <  2:1814977/149‑1 (MQ=255)
                                                                                                         gTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTa                                      <  2:251730/149‑1 (MQ=255)
                                                                                                          tGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTAt                                     >  1:2659865/1‑149 (MQ=255)
                                                                                                          tGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTAt                                     >  2:1497608/1‑149 (MQ=255)
                                                                                                           gCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATc                                    >  1:1340992/1‑149 (MQ=255)
                                                                                                           gCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATc                                    >  1:1862871/1‑149 (MQ=255)
                                                                                                               cttTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATc                                <  2:3340062/149‑1 (MQ=255)
                                                                                                               cttTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATc                                >  1:759875/1‑149 (MQ=255)
                                                                                                               cttTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATc                                <  2:2631659/149‑1 (MQ=255)
                                                                                                               cttTTAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATc                                >  1:4058413/1‑149 (MQ=255)
                                                                                                                 tttAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTg                              <  1:3013996/149‑1 (MQ=255)
                                                                                                                  ttAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGa                             >  1:149309/1‑149 (MQ=255)
                                                                                                                  ttAATTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGa                             <  2:2721876/149‑1 (MQ=255)
                                                                                                                    aaTTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAAc                           <  2:3260924/149‑1 (MQ=255)
                                                                                                                     aTTCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACa                          >  2:1907755/1‑149 (MQ=255)
                                                                                                                      ttCGGTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCCACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACaa                         <  2:672948/149‑1 (MQ=255)
                                                                                                                          gTACGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGAc                     >  1:3850641/1‑149 (MQ=255)
                                                                                                                             cGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTc                  <  1:710284/149‑1 (MQ=255)
                                                                                                                             cGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTc                  <  1:379192/149‑1 (MQ=255)
                                                                                                                             cGGTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTc                  >  1:3412789/1‑149 (MQ=255)
                                                                                                                              ggTCGTCGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTcc                 <  1:1204640/149‑1 (MQ=255)
                                                                                                                               gtcgtcGCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCt                >  2:4037560/1‑149 (MQ=255)
                                                                                                                                tcgacgCCATCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTTGTTACTGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTg               <  2:2411833/149‑1 (MQ=255)
                                                                                                                                 cgtcgCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGa              <  2:2192099/149‑1 (MQ=255)
                                                                                                                                  gtcgCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGAc             <  1:162896/149‑1 (MQ=255)
                                                                                                                                   tcgCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGAcc            >  1:1121130/1‑149 (MQ=255)
                                                                                                                                     gCCTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCtt          <  2:1182510/149‑1 (MQ=255)
                                                                                                                                      ccTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTc         >  2:3498728/1‑149 (MQ=255)
                                                                                                                                       cTTCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCa        <  2:3596114/149‑1 (MQ=255)
                                                                                                                                        ttCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCAc       >  1:96373/1‑149 (MQ=255)
                                                                                                                                        ttCGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCAGAACAATGACTTCCTGACCTTCAc       <  1:2558062/149‑1 (MQ=255)
                                                                                                                                          cGATACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGc     <  1:3891129/149‑1 (MQ=255)
                                                                                                                                            aTACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCaa   <  1:1679611/149‑1 (MQ=255)
                                                                                                                                            aTACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCaa   <  1:2845061/149‑1 (MQ=255)
                                                                                                                                            aTACGGAGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCaa   >  2:1983093/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAAC  >  CP009273/1139256‑1139545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑