Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,224,292 G→A 100% D8N (GAT→AAT)  ycgN → UPF0153 family cysteine cluster protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,224,2920GA99.7% 828.3 / ‑6.1 247D8N (GAT→AAT) ycgNUPF0153 family cysteine cluster protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (109/137);  minor base C (1/0);  total (110/137)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 247 total aligned reads displayed.

GCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTCG  >  CP009273/1224146‑1224437
                                                                                                                                                  |                                                                                                                                                 
gCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAAt                                                                                                                                                 <  1:2909639/149‑1 (MQ=255)
gCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAAt                                                                                                                                                 >  1:2659807/1‑149 (MQ=255)
  tGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGt                                                                                                                                               <  1:3808666/149‑1 (MQ=255)
   gACAGCCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTa                                                                                                                                              >  1:2751812/1‑149 (MQ=255)
    aCAGTCACTTTCGCTGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTAc                                                                                                                                             <  2:3859688/149‑1 (MQ=255)
    aCAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTaa                                                                                                                                             <  1:1099043/149‑2 (MQ=255)
    aCAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTAc                                                                                                                                             <  2:1368763/149‑1 (MQ=255)
         cACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTc                                                                                                                                        <  2:2849336/149‑1 (MQ=255)
           cTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTg                                                                                                                                      <  2:1131704/149‑1 (MQ=255)
             ttCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGc                                                                                                                                    <  2:2698077/149‑1 (MQ=255)
                 aTGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAg                                                                                                                                >  2:2022948/1‑149 (MQ=255)
                    ggCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTaa                                                                                                                             >  2:2243828/1‑149 (MQ=255)
                    ggCATTCTGTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTaa                                                                                                                             <  2:3156355/149‑1 (MQ=255)
                     gCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTaaa                                                                                                                            <  1:1680693/149‑1 (MQ=255)
                     gCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTaaa                                                                                                                            <  2:1775865/149‑1 (MQ=255)
                     gCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTaaa                                                                                                                            >  1:1016373/1‑149 (MQ=255)
                      cATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTaaaa                                                                                                                           <  1:4006736/149‑1 (MQ=255)
                       aTTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAc                                                                                                                          <  1:2728441/149‑1 (MQ=255)
                       aTTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAc                                                                                                                          <  1:4059340/149‑1 (MQ=255)
                         tCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAccc                                                                                                                        <  2:2964359/149‑1 (MQ=255)
                          cTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCt                                                                                                                       <  1:1934075/149‑1 (MQ=255)
                           tttGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTg                                                                                                                      <  1:2968229/149‑1 (MQ=255)
                           tttGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTg                                                                                                                      <  1:145483/149‑1 (MQ=255)
                            ttGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTgg                                                                                                                     <  2:1243808/149‑1 (MQ=255)
                            ttGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTgg                                                                                                                     <  2:3473424/149‑1 (MQ=255)
                              gACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGAc                                                                                                                   <  1:242791/149‑1 (MQ=255)
                              gACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGAc                                                                                                                   >  2:1450035/1‑149 (MQ=255)
                              gACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGAc                                                                                                                   <  2:1578714/149‑1 (MQ=255)
                              gACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGAc                                                                                                                   <  2:1835113/149‑1 (MQ=255)
                                 aaCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGaa                                                                                                                >  2:3263787/1‑149 (MQ=255)
                                   cTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAAt                                                                                                              <  1:3569098/149‑1 (MQ=255)
                                   cTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAAt                                                                                                              <  2:1641226/149‑1 (MQ=255)
                                    tCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATg                                                                                                             <  2:3200300/149‑1 (MQ=255)
                                     cgcgTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGa                                                                                                            <  1:3409186/149‑1 (MQ=255)
                                           ttGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATg                                                                                                      >  2:727966/1‑149 (MQ=255)
                                                aTACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGaa                                                                                                 <  2:1092615/149‑1 (MQ=255)
                                                aTACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGaa                                                                                                 <  1:381599/149‑1 (MQ=255)
                                                    tttttGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATggg                                                                                             >  1:1282519/1‑149 (MQ=255)
                                                      tttGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTGCCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAg                                                                                           <  1:3202148/149‑1 (MQ=255)
                                                      tttGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAg                                                                                           <  2:1962008/149‑1 (MQ=255)
                                                         gccgccTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCg                                                                                        >  2:2453735/1‑149 (MQ=255)
                                                               tGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTtgtgt                                                                                  <  2:3417863/149‑1 (MQ=255)
                                                                gAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTtgtttg                                                                                 >  1:1187076/1‑149 (MQ=255)
                                                                gAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTtgtgtg                                                                                 <  2:1376707/149‑1 (MQ=255)
                                                                  aaGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGAt                                                                               <  2:531980/149‑1 (MQ=255)
                                                                  aaGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGAt                                                                               <  1:2099264/149‑1 (MQ=255)
                                                                   aGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATg                                                                              <  2:590277/149‑1 (MQ=255)
                                                                   aGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATg                                                                              >  2:1576383/1‑149 (MQ=255)
                                                                   aGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATg                                                                              >  2:3627846/1‑149 (MQ=255)
                                                                    gcggcgGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATgg                                                                             <  2:1900910/149‑1 (MQ=255)
                                                                     cggcggCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGt                                                                            <  1:3747087/149‑1 (MQ=255)
                                                                      ggcggcAAAACTTGCATCGCTGTGCCAGAATGGTTATAATGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGAGATGCGGAATGGGAGTCGTGGTGtgtttgtt                                                                           >  1:488930/1‑149 (MQ=255)
                                                                        cggcAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGc                                                                         <  1:2607546/149‑1 (MQ=255)
                                                                          gcAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCgg                                                                       >  1:2331586/1‑149 (MQ=255)
                                                                             aaaCTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCACAGTAAAACCCTGGACGAAAGGAGCGATGCGGACTGGGAGTCGTTGTGTGATGGTTGCGGTCa                                                                    >  2:4014961/1‑149 (MQ=255)
                                                                             aaaCTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCa                                                                    >  2:1252435/1‑149 (MQ=255)
                                                                                  tGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGtt                                                               <  1:1576383/149‑1 (MQ=255)
                                                                                  tGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGtt                                                               <  1:3627846/149‑1 (MQ=255)
                                                                                  tGCATCGCTGTGCCAGACTGGGTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGtt                                                               <  2:3852258/149‑1 (MQ=255)
                                                                                   gCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTg                                                              >  2:3109282/1‑149 (MQ=255)
                                                                                     aTCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCAGATTAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGAGGAATGGGAGTCGTTGTTTGATGGTTGAGGTAAGTGtttgc                                                            >  1:1881297/1‑146 (MQ=255)
                                                                                      tCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCt                                                           >  1:3078643/1‑149 (MQ=255)
                                                                                         cTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCa                                                        <  2:1327350/149‑1 (MQ=255)
                                                                                              ccAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAAc                                                   <  2:321878/149‑1 (MQ=255)
                                                                                              ccAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAAc                                                   >  1:1617945/1‑149 (MQ=255)
                                                                                              ccAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAAc                                                   <  2:3948362/149‑1 (MQ=255)
                                                                                               cAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACt                                                  >  1:1374394/1‑149 (MQ=255)
                                                                                                    tGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTgatgg                                             >  2:1733198/1‑149 (MQ=255)
                                                                                                    tGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTgatgg                                             >  1:1718611/1‑149 (MQ=255)
                                                                                                     ggTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATTGTTTCGGTCAGTGTTTCCTGCATAAACAgattga                                            >  1:3937119/1‑149 (MQ=255)
                                                                                                      gTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTgatggat                                           >  2:1082479/1‑149 (MQ=255)
                                                                                                      gTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTgatggat                                           <  2:1343721/149‑1 (MQ=255)
                                                                                                      gTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGCGAGTCGTTGTGTGTTGGGTGCGGTCAGTGTTGCCTGCATAAACAgatggat                                           >  1:3919423/1‑149 (MQ=255)
                                                                                                        tataAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAGTGGGAGTCGTTGTGTGTGGGTTGCGGTCAGTGTTGCCTGCAGAAACTGGTGGATGa                                         >  1:2102748/1‑149 (MQ=255)
                                                                                                        tataAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCCGTGTTGGCTGCATAAACAGATGGATGa                                         >  1:3872942/1‑149 (MQ=255)
                                                                                                         ataAGGAGCCTTTTAACGTAATGGAGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCTGAATGGGAGTCGTTGAGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACAGATGGATGaa                                        >  2:2983579/1‑149 (MQ=255)
                                                                                                           aaGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGa                                      <  2:2750957/149‑1 (MQ=255)
                                                                                                             ggTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGaca                                    >  1:998247/1‑149 (MQ=255)
                                                                                                             ggTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGaca                                    >  1:2239354/1‑149 (MQ=255)
                                                                                                              gTGCGTTTTAACGTAATGGCGGAACAACTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGacac                                   <  2:3131056/149‑1 (MQ=255)
                                                                                                               tGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACAcc                                  >  2:698899/1‑149 (MQ=255)
                                                                                                                      tAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGaaa                           <  1:2612630/149‑1 (MQ=255)
                                                                                                                      tAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGaaa                           <  1:1309269/149‑1 (MQ=255)
                                                                                                                        aCGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATc                         <  1:782342/149‑1 (MQ=255)
                                                                                                                          gTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTa                       <  2:2626557/149‑1 (MQ=255)
                                                                                                                           tAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTAc                      >  2:2942730/1‑149 (MQ=255)
                                                                                                                               ggCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCa                  >  2:3579483/1‑149 (MQ=255)
                                                                                                                                gCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCAc                 <  2:1914871/149‑1 (MQ=255)
                                                                                                                                gCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCAc                 <  2:3141883/149‑1 (MQ=255)
                                                                                                                                  ggAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTa               >  2:2409691/1‑149 (MQ=255)
                                                                                                                                   gAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTaa              >  1:2530458/1‑149 (MQ=255)
                                                                                                                                   gAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCAAAAACAGATGGATGAAGACACCCACGAAATCTAATTAAATaa              >  1:142564/1‑149 (MQ=255)
                                                                                                                                    aaCACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAAc             <  2:2103224/149‑1 (MQ=255)
                                                                                                                                    aaCACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGATTGTTGCGGTCCGTGTTGCCTGCCTAAAATGATGGATTAAGACACCGCCGAAATATAATtaactaac             >  1:1421274/1‑149 (MQ=255)
                                                                                                                                       acCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCCTAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTc          >  1:2018574/1‑149 (MQ=255)
                                                                                                                                         cTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGc        <  2:2659807/149‑1 (MQ=255)
                                                                                                                                          tgatgaGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGcc       <  1:106544/149‑1 (MQ=255)
                                                                                                                                          tgatgaGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGcc       <  1:105139/149‑1 (MQ=255)
                                                                                                                                           gatgaGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGGTGGTTGCGGTCAGTGTTGCCTGCATAAACTGGTTGATGAAGACACCCACGAAATCTACATCACTAACGTCGccg      >  1:3124451/1‑148 (MQ=255)
                                                                                                                                             tgaGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGTTGGTTGCGGTCAGTGGTGGCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTAACTAACGTCGCCTGt    >  1:2836117/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTCG  >  CP009273/1224146‑1224437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑