BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,259,343	C→A	6.5%	S58R (AGC→AGA)	hemA →	glutamyl tRNA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,259,343	0	C	A	6.5%	1023.6 / 27.7	276	S58R (AGC→AGA)	hemA	glutamyl tRNA reductase
Reads supporting (aligned to +/- strand): ref base C (114/144); new base A (13/5); total (127/149)
Fisher's exact test for biased strand distribution p-value = 2.70e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

Only 100 of 277 total aligned reads displayed.

ACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTG  >  CP009273/1259195‑1259490

aCCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGc                                                                                                                                                     <  2:407368/149‑1 (MQ=255)

  cATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGt                                                                                                                                                   <  2:1786474/149‑1 (MQ=255)

   aTAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGtt                                                                                                                                                  <  2:491500/149‑1 (MQ=255)

    tAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTg                                                                                                                                                 >  1:4037624/1‑149 (MQ=255)

      aaaCGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGaa                                                                                                                                               <  1:968449/149‑1 (MQ=255)

       aaCGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAag                                                                                                                                              >  2:453892/1‑149 (MQ=255)

        aCGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAaga                                                                                                                                             <  2:71173/149‑1 (MQ=255)

         cGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAagag                                                                                                                                            <  1:3486055/149‑1 (MQ=255)

           gCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGGTGAAGAGCa                                                                                                                                          >  2:842883/1‑149 (MQ=255)

              ccTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGa                                                                                                                                       <  2:1179432/149‑1 (MQ=255)

                tGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACa                                                                                                                                     >  1:863262/1‑149 (MQ=255)

                tGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACa                                                                                                                                     >  1:3184937/1‑149 (MQ=255)

                  tATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAAc                                                                                                                                   <  1:1457578/149‑1 (MQ=255)

                   aTCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGAGTTGAAGAGCAGGACAAcc                                                                                                                                  <  1:4095349/149‑1 (MQ=255)

                   aTCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGAGTTGAAGAGCAGGACAAcc                                                                                                                                  <  1:697450/149‑1 (MQ=255)

                    tCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCt                                                                                                                                 >  1:3327155/1‑149 (MQ=255)

                        tGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCaa                                                                                                                             >  1:1014615/1‑149 (MQ=255)

                          cGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAaga                                                                                                                           <  2:3919938/149‑1 (MQ=255)

                          cGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAaga                                                                                                                           <  1:329755/149‑1 (MQ=255)

                          cGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAaga                                                                                                                           >  1:1306184/1‑149 (MQ=255)

                           gagaACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAagag                                                                                                                          <  1:3834849/149‑1 (MQ=255)

                            agaACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAgg                                                                                                                         >  1:2129326/1‑149 (MQ=255)

                            agaACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAgg                                                                                                                         >  1:166452/1‑149 (MQ=255)

                             gaACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGc                                                                                                                        <  1:2818418/149‑1 (MQ=255)

                             gaACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGc                                                                                                                        <  1:1623164/149‑1 (MQ=255)

                             gaACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGc                                                                                                                        >  1:362730/1‑149 (MQ=255)

                              aaCGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCg                                                                                                                       >  1:2969460/1‑149 (MQ=255)

                                   gtATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAAt                                                                                                                  <  2:1002268/149‑1 (MQ=255)

                                   gtATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGAGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAAt                                                                                                                  >  2:3973683/1‑149 (MQ=255)

                                       cGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGc                                                                                                              <  2:1363709/149‑1 (MQ=255)

                                       cGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGc                                                                                                              <  2:1538457/149‑1 (MQ=255)

                                       cGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGc                                                                                                              <  1:3222739/149‑1 (MQ=255)

                                        gTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCt                                                                                                             >  1:2052425/1‑149 (MQ=255)

                                         ttttCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTg                                                                                                            >  1:242700/1‑149 (MQ=255)

                                           ttCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGc                                                                                                          <  1:2900787/149‑1 (MQ=255)

                                            tCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGAGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCt                                                                                                         <  1:1112141/149‑1 (MQ=255)

                                                cGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGc                                                                                                     <  1:3387328/149‑1 (MQ=255)

                                                cGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGAGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGc                                                                                                     <  1:3926076/149‑1 (MQ=255)

                                                 ggATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCg                                                                                                    <  2:642111/149‑1 (MQ=255)

                                                 ggATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCg                                                                                                    <  2:641370/149‑1 (MQ=255)

                                                  gATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGa                                                                                                   >  2:3323591/1‑149 (MQ=255)

                                                     aaGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTa                                                                                                <  2:3367431/149‑1 (MQ=255)

                                                      aGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGAGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTAt                                                                                               >  2:350804/1‑149 (MQ=255)

                                                        cTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATca                                                                                             <  2:1380272/149‑1 (MQ=255)

                                                        cTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATca                                                                                             <  2:2036265/149‑1 (MQ=255)

                                                        cTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATca                                                                                             >  2:2836870/1‑149 (MQ=255)

                                                        cTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATca                                                                                             <  2:419744/149‑1 (MQ=255)

                                                           gATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCTTTAATCCGCTGGCTTTGCGATTATCACaa                                                                                          <  1:2645971/149‑1 (MQ=255)

                                                           gATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACaa                                                                                          <  2:2550307/149‑1 (MQ=255)

                                                            aTCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAAt                                                                                         <  1:2682508/149‑1 (MQ=255)

                                                            aTCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAAt                                                                                         >  1:783391/1‑149 (MQ=255)

                                                            aTCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAAt                                                                                         <  2:2247540/149‑1 (MQ=255)

                                                                ggCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTa                                                                                     >  1:1921691/1‑149 (MQ=255)

                                                                 gcgcTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTaa                                                                                    <  1:1614630/149‑1 (MQ=255)

                                                                  cgcTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAAt                                                                                   <  1:84424/149‑1 (MQ=255)

                                                                   gcTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATg                                                                                  <  1:322983/149‑1 (MQ=255)

                                                                   gcTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATg                                                                                  >  2:1062/1‑149 (MQ=255)

                                                                      tGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATgaag                                                                               <  2:1648205/149‑1 (MQ=255)

                                                                       gACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATgaaga                                                                              >  2:3720375/1‑149 (MQ=255)

                                                                        aCAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATgaagaa                                                                             <  1:1932616/149‑1 (MQ=255)

                                                                          aGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAagaaga                                                                           <  2:1897109/149‑1 (MQ=255)

                                                                          aGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAagaaga                                                                           >  1:1406156/1‑149 (MQ=255)

                                                                           gCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGAt                                                                          <  2:242700/149‑1 (MQ=255)

                                                                            ccTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATc                                                                         <  2:2661688/149‑1 (MQ=255)

                                                                              tGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTg                                                                       >  2:1924895/1‑149 (MQ=255)

                                                                               gCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGc                                                                      <  2:1648524/149‑1 (MQ=255)

                                                                               gCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGc                                                                      >  1:3048334/1‑149 (MQ=255)

                                                                                    cgcAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTaaa                                                                 >  1:3730647/1‑149 (MQ=255)

                                                                                     gcAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTaaaa                                                                <  1:288970/149‑1 (MQ=255)

                                                                                     gcAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTaaaa                                                                <  2:2317180/149‑1 (MQ=255)

                                                                                        gCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGcc                                                             <  2:1755697/149‑1 (MQ=255)

                                                                                           gATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGAGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCctct                                                          >  1:1235408/1‑149 (MQ=255)

                                                                                            aTGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTa                                                         >  1:1890009/1‑149 (MQ=255)

                                                                                             tGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTAc                                                        <  2:2979607/149‑1 (MQ=255)

                                                                                                 gCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGAGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGc                                                    >  1:2270322/1‑149 (MQ=255)

                                                                                                        ggcgTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGa                                             <  2:3327155/149‑1 (MQ=255)

                                                                                                         gcgTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGAt                                            >  1:3171903/1‑149 (MQ=255)

                                                                                                           gtggtgCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATaa                                          <  2:3263783/149‑1 (MQ=255)

                                                                                                                     cGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGtt                                <  2:2421011/149‑1 (MQ=255)

                                                                                                                      gACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTa                               >  1:1427000/1‑149 (MQ=255)

                                                                                                                        cGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGc                             <  2:3485734/149‑1 (MQ=255)

                                                                                                                        cGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGc                             <  2:543402/149‑1 (MQ=255)

                                                                                                                          tGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTAGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCa                           >  1:181186/1‑149 (MQ=255)

                                                                                                                           gCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCAt                          <  1:829703/149‑1 (MQ=255)

                                                                                                                               ccGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCATTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTaa                      <  1:678075/149‑1 (MQ=255)

                                                                                                                                 gCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATg                    >  1:2602682/1‑149 (MQ=255)

                                                                                                                                  cACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATTACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGc                   >  2:1711787/1‑149 (MQ=255)

                                                                                                                                  cACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGc                   >  1:1529037/1‑149 (MQ=255)

                                                                                                                                  cACGGAACTTTATCTTAGAGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGc                   >  1:2487019/1‑149 (MQ=255)

                                                                                                                                   aCGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg                  >  2:3313971/1‑149 (MQ=255)

                                                                                                                                   aCGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCg                  >  2:2511437/1‑149 (MQ=255)

                                                                                                                                    cGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCgt                 <  2:2137983/149‑1 (MQ=255)

                                                                                                                                    cGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCgt                 >  2:1851830/1‑149 (MQ=255)

                                                                                                                                    cGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCgt                 >  1:1759034/1‑149 (MQ=255)

                                                                                                                                      gAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCgtgt               <  1:3702527/149‑1 (MQ=255)

                                                                                                                                         cTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGc            <  2:2089790/149‑1 (MQ=255)

                                                                                                                                         cTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGc            <  2:2599571/149‑1 (MQ=255)

                                                                                                                                          tttATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCTTTAATCCGCTGGCTTTGCGTTTATCAAAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGcc           <  1:207269/149‑1 (MQ=255)

                                                                                                                                           ttATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCa          <  2:1964289/149‑1 (MQ=255)

                                                                                                                                            tATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAg         >  1:900377/1‑149 (MQ=255)

ACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTG  >  CP009273/1259195‑1259490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`