Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 107,220 A→T 15.2% K819* (AAA→TAA)  secA → preprotein translocase subunit, ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273107,2200AT15.2% 554.9 / 18.1 221K819* (AAA→TAA) secApreprotein translocase subunit, ATPase
Reads supporting (aligned to +/- strand):  ref base A (56/123);  new base T (27/2);  total (96/125)
Fisher's exact test for biased strand distribution p-value = 1.67e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

AACGCTTGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGC  >  CP009273/107072‑107368
                                                                                                                                                    |                                                                                                                                                    
aaCGCTTGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGa                                                                                                                                                      >  2:2715864/1‑149 (MQ=255)
   gCTTGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGtaag                                                                                                                                                   >  1:4090836/1‑148 (MQ=255)
   gCTTGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAat                                                                                                                                                   <  1:3763903/149‑1 (MQ=255)
    cTTGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAata                                                                                                                                                  <  2:1542591/149‑1 (MQ=255)
       gACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGTAatattt                                                                                                                                               >  1:275619/1‑147 (MQ=255)
         cTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAACTATGAAg                                                                                                                                             >  2:2545282/1‑149 (MQ=255)
         cTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAg                                                                                                                                             <  1:871396/149‑1 (MQ=255)
         cTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAg                                                                                                                                             <  2:2624417/149‑1 (MQ=255)
          tCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGt                                                                                                                                            <  1:1777372/149‑1 (MQ=255)
          tCCCTGTGGAAAGAGCACCCGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGt                                                                                                                                            <  2:776595/149‑1 (MQ=255)
           cccTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGtt                                                                                                                                           >  2:331665/1‑149 (MQ=255)
             cTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTAt                                                                                                                                         <  1:3856936/149‑1 (MQ=255)
               gtgGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCa                                                                                                                                       <  1:322286/149‑1 (MQ=255)
                tgGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAg                                                                                                                                      <  1:2753544/149‑1 (MQ=255)
                 ggAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGTGATATGAAGTTATAAga                                                                                                                                     >  1:15636/1‑148 (MQ=255)
                 ggAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGAAATATGAAGTTATAAGt                                                                                                                                     >  1:1242909/1‑149 (MQ=255)
                  gAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCAACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGCAATATGAAGTTATAAGTa                                                                                                                                    >  2:3534134/1‑149 (MQ=255)
                     agagCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGc                                                                                                                                 <  2:3473544/149‑1 (MQ=255)
                     agagCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGc                                                                                                                                 >  2:4049756/1‑149 (MQ=255)
                       agCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTg                                                                                                                               <  1:2986933/149‑1 (MQ=255)
                             tGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCaaa                                                                                                                         <  2:546911/149‑1 (MQ=255)
                              ggCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAg                                                                                                                        <  1:3450845/149‑1 (MQ=255)
                               gCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGt                                                                                                                       <  2:3071332/149‑1 (MQ=255)
                                       ggACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTAc                                                                                                               >  2:2104101/1‑149 (MQ=255)
                                       ggACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTAc                                                                                                               <  2:3715890/149‑1 (MQ=255)
                                         aCTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGt                                                                                                             <  1:931660/149‑1 (MQ=255)
                                             tCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGTAATATGAAGGTATCAGTACGCTGAGCCAAGTTCAGGTACGTATGc                                                                                                         >  2:4055313/1‑149 (MQ=255)
                                             tCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGAAATATTAAGTTATAAGTACGCTGAGCAAAGTTCAGGTACGTATGc                                                                                                         >  1:915990/1‑149 (MQ=255)
                                               tGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCt                                                                                                       <  2:1665076/149‑1 (MQ=255)
                                                   tCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAag                                                                                                   >  2:1324375/1‑149 (MQ=255)
                                                    cAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGTAATATTAAGTTATAAGTACGCTGAGCAAATTTCAGGTACGGATGCCGGgaga                                                                                                  >  1:617054/1‑149 (MQ=255)
                                                     aGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAagag                                                                                                 <  1:3371638/149‑1 (MQ=255)
                                                       ggTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGt                                                                                               <  2:698425/149‑1 (MQ=255)
                                                         tATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGGAATATGTATTTATCAGTAAGCGGGGCCAAGTTAAGGTACGGATTCCGGTAGAGGTTg                                                                                             >  1:853355/1‑149 (MQ=255)
                                                          aTCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTga                                                                                            <  2:619613/149‑1 (MQ=255)
                                                           tCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTgag                                                                                           <  1:3533490/149‑1 (MQ=255)
                                                           tCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTgag                                                                                           <  1:2614300/149‑1 (MQ=255)
                                                           tCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTgag                                                                                           <  2:1386251/149‑1 (MQ=255)
                                                           tCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTgag                                                                                           <  2:3257995/149‑1 (MQ=255)
                                                           tCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTgag                                                                                           <  1:3506592/149‑1 (MQ=255)
                                                             cACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTgagga                                                                                         <  1:3032154/149‑1 (MQ=255)
                                                              aCCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGTAATATTAATTTATAAGTACGCTGAGCAAACTTAAGGTACGTATGCCTGTAGAGGTTgaggag                                                                                        >  1:3006445/1‑149 (MQ=255)
                                                              aCCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGGAATATGAAGTTTTAAGTAAGCTGTGCAAAATTAAGGTGCGGATGCCGGGAGAGGTTggggcg                                                                                        >  1:380409/1‑147 (MQ=255)
                                                               ccTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGc                                                                                       <  2:3433255/149‑1 (MQ=255)
                                                               ccTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGc                                                                                       <  1:2538912/149‑1 (MQ=255)
                                                                    gTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGTAATATTTATTTATAAGTAAGCTGAGCAAAATTGATGTACGGATGCCGGCAGAGGGTGAGGTGCgggta                                                                                  >  1:423977/1‑147 (MQ=255)
                                                                      ggCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATAAGTACGCTGAGCAAAGTTAAGGTACGTATGCCTGCAGAGGTTGAGGTGGTGGaaca                                                                                >  2:1793172/1‑149 (MQ=255)
                                                                          aCGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGTAATATTAAGTTATAAGTAAGCTGAGCAAAGTTCAGGTACGTATGCCGGAAGAGGTTGTGGAGCTGGAACAAAAg                                                                            >  1:1352586/1‑149 (MQ=255)
                                                                               caGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGTAATATTAAGTTATAATTACGCGGAGCAAAGTTCAGGGACGTATGCCGGAAGAGGTTGTGGAGCTGGAACAACCgcggcg                                                                       >  1:471795/1‑149 (MQ=255)
                                                                                aGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGGAATATGAAGTTATAAGTACGCGGGGCAAAGTTCAGGTACGTATTCCTGAAGAGGTTGTGGTGCTGGAACAACATcgtcgt                                                                      >  1:3096557/1‑149 (MQ=255)
                                                                                aGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGGTGAAATATGTATTTATCAGTACGCGGTGCAAAGTTCAGGTACGTCTGCCGGAAGAGGGTGAGGAGCTGGAACAACAGcgtcgt                                                                      >  2:490064/1‑149 (MQ=255)
                                                                                 gAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTa                                                                     <  1:1793172/149‑1 (MQ=255)
                                                                                 gAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTa                                                                     <  2:2995148/149‑1 (MQ=255)
                                                                                  aaaGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTAt                                                                    <  2:2424730/149‑1 (MQ=255)
                                                                                   aaGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATg                                                                   <  1:1132341/149‑1 (MQ=255)
                                                                                   aaGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGTAATATGAAGTTATAAGTAAGCGGAGCCAAGTTCAGGTACGGATGGCTGAAGAGGGTGTGGTGCTGGGAAAACACCGGCGGATg                                                                   >  1:2797856/1‑149 (MQ=255)
                                                                                    aGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGAAATATGAAGTTATCAGTAAGCTGGGCAAAGTTAAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATgg                                                                  >  1:1991084/1‑149 (MQ=255)
                                                                                     gATCCGAAGCAGGAATACACACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGTAGTCGTTTTAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGa                                                                 <  2:1070072/149‑1 (MQ=255)
                                                                                      aTCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGaa                                                                <  1:2965526/149‑1 (MQ=255)
                                                                                       tCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAg                                                               <  1:1672989/149‑1 (MQ=255)
                                                                                       tCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAg                                                               <  2:3343412/149‑1 (MQ=255)
                                                                                        ccGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGTAATATGAATTTATCAGTACGCTGTGCAAAGTTCAGGTACGTATGCCAGTAGAGGTTGTGGAGCTGGAAAAACAGCGTCGTATGGAAGc                                                              >  1:2040603/1‑149 (MQ=255)
                                                                                         cGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGcc                                                             <  2:3960618/149‑1 (MQ=255)
                                                                                         cGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGAAATATTAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGTAGAGGGTGTGGAGCGGGTACAACAGCGTCGTATGGAAGcc                                                             >  1:319261/1‑149 (MQ=255)
                                                                                          gAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCg                                                            <  2:2010733/149‑1 (MQ=255)
                                                                                           aaGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGa                                                           <  2:3113378/149‑1 (MQ=255)
                                                                                            aGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAg                                                          <  1:3672782/149‑1 (MQ=255)
                                                                                             gCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGc                                                         <  1:2411960/149‑1 (MQ=255)
                                                                                               aGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGt                                                       <  1:769611/149‑1 (MQ=255)
                                                                                               aGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGt                                                       <  1:53619/149‑1 (MQ=255)
                                                                                                ggAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGTAATATGAAGTTATAAGTAAGCTGAGCAAAATTAAGGTAAGTATTCCGGAAGAGGTTGAGGAGCTGGAACAAAACCCGCGTATGGAAGCCGAGCGtt                                                      >  1:3537028/1‑149 (MQ=255)
                                                                                                   aTACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAg                                                   <  1:2142694/149‑1 (MQ=255)
                                                                                                    tACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGTAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGGTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGGTTatc                                                  >  2:1935614/1‑147 (MQ=255)
                                                                                                    tACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAgc                                                  <  1:2264061/149‑1 (MQ=255)
                                                                                                      cAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGTGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAgcgc                                                >  2:2038431/1‑149 (MQ=255)
                                                                                                         aCGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCaaa                                             <  2:3772616/149‑1 (MQ=255)
                                                                                                         aCGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCaaa                                             <  2:2142420/149‑1 (MQ=255)
                                                                                                         aCGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCGGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCaaa                                             <  2:1686830/149‑1 (MQ=255)
                                                                                                            tGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGTAGAGGTTGTGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAAttc                                          >  2:675359/1‑147 (MQ=255)
                                                                                                              aaTCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGGTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATgcag                                        <  1:2262564/149‑1 (MQ=255)
                                                                                                                   ttCTACATGTTTGCAGCGATGCTGTAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCATATTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCt                                   <  2:3198947/149‑1 (MQ=255)
                                                                                                                        cATGTTTGCAGCGATGCTGGAGTCGTTGTAATATTTAGTTATAACTACGCGGAGCAAAGTTCAGGTACGGATGCCTGAAGAGGTTGTGGTGCTGGAACAACAGCGTCGTATGGAATCCGAGCGGTTATCGCAAATGCagcagcgtttcc                              >  1:2342009/1‑143 (MQ=255)
                                                                                                                        cATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGcc                              <  1:2737590/149‑1 (MQ=255)
                                                                                                                         aTGTTTGCAGCGATGCTGGAGTCGTTGAAATATTAAGTTATCAGTACGCAGAGCCAAGTTCAGGTACGTATGCCGGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCCAGCGTTTAGCGCAAATGCAGCAGCTTAGCCa                             >  2:214044/1‑149 (MQ=255)
                                                                                                                         aTGTTTGCAGCGATGCTGGAGTCGTGGTAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCGGAAGAGGTTGGGGAGCTGGAACAACAGCGTCGTGTGGAAGCCGAGCGTTTAGCGCAAATGCCGCAGCTTAGCCa                             >  2:1592993/1‑149 (MQ=255)
                                                                                                                         aTGTTTGCAGCGATGCTGGAGTCGTGGAAATATGAAGTTATAAGTACGCTGTGCCAAGTTCAGGTACGTATGCCGGAAGAGGTTGAGGAGCTGGTACAACAGCGTCGTATGGAAGCCGAGCGGTTATCGCAAATTCAGCAGCTTAGCCa                             >  2:1590631/1‑149 (MQ=255)
                                                                                                                          tGTTTGCAGCGATGCTGGAGTCGTGGTAATATGAAGTTATCAGTACGCGGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCGGGAACAACAGCGTCGTCTGGAAGCCGAGCGGTTAGCGCAAATGCAGCACCTTAGCCAt                            >  2:1488015/1‑149 (MQ=255)
                                                                                                                           gTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATc                           <  1:3989907/149‑1 (MQ=255)
                                                                                                                             ttGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAg                         <  1:999133/149‑1 (MQ=255)
                                                                                                                                 aGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATg                     <  2:4090836/149‑1 (MQ=255)
                                                                                                                                            aGTCGTTTAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTAGGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCa          <  1:2104101/149‑1 (MQ=255)
                                                                                                                                              tCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGc        >  1:3584973/1‑149 (MQ=255)
                                                                                                                                              tCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGc        >  2:1233749/1‑149 (MQ=255)
                                                                                                                                              tCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGc        >  1:1848037/1‑149 (MQ=255)
                                                                                                                                                gTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCg      >  1:2603983/1‑149 (MQ=255)
                                                                                                                                                gTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCg      >  1:3633312/1‑149 (MQ=255)
                                                                                                                                                gTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCg      >  2:3647147/1‑149 (MQ=255)
                                                                                                                                                gTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCg      >  2:3646495/1‑149 (MQ=255)
                                                                                                                                                 ttGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGc     >  1:1669290/1‑149 (MQ=255)
                                                                                                                                                 ttGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGc     >  1:1086549/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AACGCTTGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGC  >  CP009273/107072‑107368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑