Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,336,599 T→C 22.0% L141P (CTT→CCT)  pyrF → orotidine‑5'‑phosphate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,336,5990TC22.0% 504.4 / 161.8 246L141P (CTT→CCT) pyrForotidine‑5'‑phosphate decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (93/98);  new base C (21/33);  total (115/131)
Fisher's exact test for biased strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.02e-01

Only 100 of 248 total aligned reads displayed.

GTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGC  >  CP009273/1336454‑1336747
                                                                                                                                                 |                                                                                                                                                    
  gtgGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCa                                                                                                                                                 >  1:209015/1‑149 (MQ=255)
   tgGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACGGGTGCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCACCAGGGAAGCCAGCGACCTGGTCGATCTTGGCAt                                                                                                                                                <  1:1741965/149‑1 (MQ=255)
     gATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGa                                                                                                                                              >  1:2325250/1‑149 (MQ=255)
     gATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCCTGGCATGa                                                                                                                                              >  1:1069052/1‑149 (MQ=255)
     gATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCCTGGCATGa                                                                                                                                              <  1:2071462/149‑1 (MQ=255)
      aTGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCCTGGCATGac                                                                                                                                             <  1:1331062/149‑1 (MQ=255)
       tGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGaca                                                                                                                                            >  2:2764788/1‑149 (MQ=255)
          tGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCCTGGCATGACACTg                                                                                                                                         >  1:2503207/1‑149 (MQ=255)
          tGAATGGTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTg                                                                                                                                         <  2:3071012/149‑1 (MQ=255)
           gAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGt                                                                                                                                        <  2:3356649/149‑1 (MQ=255)
            aaTGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTc                                                                                                                                       <  1:3604407/149‑1 (MQ=255)
              tGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCAc                                                                                                                                     >  2:3273312/1‑149 (MQ=255)
              tGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCAc                                                                                                                                     >  2:1062275/1‑149 (MQ=255)
                ttCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATTACACTGTCAcca                                                                                                                                   >  1:10377/1‑148 (MQ=255)
                ttCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCCTGGCATGACACTGTCACCt                                                                                                                                   <  2:3593807/149‑1 (MQ=255)
                    tGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAg                                                                                                                               >  2:3727856/1‑149 (MQ=255)
                    tGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCCTGGCATGACACTGTCACCTGCAg                                                                                                                               >  1:3366937/1‑149 (MQ=255)
                      ccTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGAt                                                                                                                             >  1:1506103/1‑149 (MQ=255)
                         ctGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTAt                                                                                                                          <  1:1971912/149‑1 (MQ=255)
                         ctGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTAt                                                                                                                          <  1:2240531/149‑1 (MQ=255)
                          tggtggGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATg                                                                                                                         <  2:614499/149‑1 (MQ=255)
                          tggtggGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATg                                                                                                                         >  1:464131/1‑149 (MQ=255)
                           ggtggGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGc                                                                                                                        >  2:871298/1‑149 (MQ=255)
                             tggGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAg                                                                                                                      <  1:1528873/149‑1 (MQ=255)
                             tggGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCCTGGCATGACACTGTCACCTGCAGATTATGCAg                                                                                                                      <  1:1207513/149‑1 (MQ=255)
                               gggCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGaa                                                                                                                    <  2:2321710/149‑1 (MQ=255)
                               gggCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGAGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGaa                                                                                                                    >  2:4022069/1‑149 (MQ=255)
                                         gatgaCCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTggcgg                                                                                                          >  1:476509/1‑149 (MQ=255)
                                          atgaCCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCCTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTggcggc                                                                                                         >  1:1117330/1‑149 (MQ=255)
                                                  aGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGc                                                                                                 <  1:3642552/149‑1 (MQ=255)
                                                    cgcgTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCCTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCaa                                                                                               <  2:205681/149‑1 (MQ=255)
                                                    cgcgTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCCTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCaa                                                                                               <  2:1903169/149‑1 (MQ=255)
                                                      cgTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCaaaa                                                                                             <  2:2506441/149‑1 (MQ=255)
                                                        tGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAAt                                                                                           <  2:86281/149‑1 (MQ=255)
                                                        tGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAAt                                                                                           <  2:315077/149‑1 (MQ=255)
                                                          aGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAAtgt                                                                                         <  2:1240332/149‑1 (MQ=255)
                                                           ggCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAAtgtg                                                                                        >  2:3558791/1‑149 (MQ=255)
                                                               cTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCt                                                                                    <  1:2870173/149‑1 (MQ=255)
                                                               cTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCt                                                                                    >  2:3510586/1‑149 (MQ=255)
                                                                tGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCtt                                                                                   <  2:3324251/149‑1 (MQ=255)
                                                                  gTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGa                                                                                 <  2:2266274/149‑1 (MQ=255)
                                                                   ttCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGAt                                                                                <  1:75868/149‑1 (MQ=255)
                                                                     ccGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGAtgg                                                                              <  2:1133299/149‑1 (MQ=255)
                                                                        tttGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGAtggtgt                                                                           <  1:2139753/149‑1 (MQ=255)
                                                                        tttGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGAtggtgt                                                                           >  2:1882062/1‑149 (MQ=255)
                                                                           ggCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGAtggtgtggt                                                                        >  2:2407002/1‑149 (MQ=255)
                                                                           ggCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGAtggtgtggt                                                                        >  2:454220/1‑149 (MQ=255)
                                                                           ggCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGAtggtgtggt                                                                        <  2:2568822/149‑1 (MQ=255)
                                                                            gCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGAtggtgtggtg                                                                       <  2:2353022/149‑1 (MQ=255)
                                                                            gCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGAtggtgtggtg                                                                       >  1:315474/1‑149 (MQ=255)
                                                                               aaGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGgtgtgt                                                                    <  2:2630628/149‑1 (MQ=255)
                                                                                  aTGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCt                                                                 >  1:889054/1‑149 (MQ=255)
                                                                                   tGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTg                                                                >  2:3618295/1‑149 (MQ=255)
                                                                                    gCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGc                                                               >  2:341910/1‑149 (MQ=255)
                                                                                       ccGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCa                                                            <  1:2176020/149‑1 (MQ=255)
                                                                                          cTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGa                                                         <  1:2736339/149‑1 (MQ=255)
                                                                                          cTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGa                                                         <  2:2865782/149‑1 (MQ=255)
                                                                                           ttttGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGaa                                                        <  2:1984966/149‑1 (MQ=255)
                                                                                           ttttGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCCTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGaa                                                        <  1:1424592/149‑1 (MQ=255)
                                                                                              tGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCt                                                     <  1:2410431/149‑1 (MQ=255)
                                                                                              tGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCCTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCt                                                     <  1:3327893/149‑1 (MQ=255)
                                                                                                aTTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCtgt                                                   <  1:1613191/149‑1 (MQ=255)
                                                                                                    cTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTgcgc                                               <  2:379314/149‑1 (MQ=255)
                                                                                                     tgtgACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCt                                              >  1:3667589/1‑149 (MQ=255)
                                                                                                     tgtgACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCt                                              <  1:168252/149‑1 (MQ=255)
                                                                                                     tgtgACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCt                                              <  1:3964592/149‑1 (MQ=255)
                                                                                                      gtgACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCCTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCtt                                             >  1:3861628/1‑149 (MQ=255)
                                                                                                      gtgACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCCTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCtt                                             >  1:3117773/1‑149 (MQ=255)
                                                                                                            gtgtTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACa                                       >  1:532384/1‑149 (MQ=255)
                                                                                                            gtgtTGACCAGCATGGAAGCCAGCGACCTGGTCGATCCTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACa                                       >  1:2738936/1‑149 (MQ=255)
                                                                                                               ttGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGt                                    <  1:1596413/149‑1 (MQ=255)
                                                                                                               ttGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGt                                    >  2:694579/1‑149 (MQ=255)
                                                                                                                tGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTa                                   <  2:3097085/149‑1 (MQ=255)
                                                                                                                 gACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTAt                                  >  2:154283/1‑149 (MQ=255)
                                                                                                                 gACCAGCATGGAAGCCAGCGACCTGGTCGATCCTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTAt                                  <  1:179208/149‑1 (MQ=255)
                                                                                                                   ccAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTc                                <  1:1661686/149‑1 (MQ=255)
                                                                                                                      gCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGt                             >  2:882735/1‑149 (MQ=255)
                                                                                                                       cATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTc                            <  1:3229333/149‑1 (MQ=255)
                                                                                                                       cATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTc                            <  2:3905430/149‑1 (MQ=255)
                                                                                                                         tGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAg                          >  1:401509/1‑149 (MQ=255)
                                                                                                                         tGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAg                          >  1:214451/1‑149 (MQ=255)
                                                                                                                         tGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAg                          >  1:903490/1‑149 (MQ=255)
                                                                                                                         tGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCGGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGGGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAg                          >  1:2472857/1‑149 (MQ=255)
                                                                                                                            aaGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAg                       <  2:720660/149‑1 (MQ=255)
                                                                                                                            aaGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAg                       >  1:1341254/1‑149 (MQ=255)
                                                                                                                              gCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGtt                     >  1:3237128/1‑149 (MQ=255)
                                                                                                                              gCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGtt                     >  1:1185999/1‑149 (MQ=255)
                                                                                                                               ccAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTc                    <  1:2098950/149‑1 (MQ=255)
                                                                                                                               ccAGCGACCTGGTCGATCCTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTc                    <  2:2503207/149‑1 (MQ=255)
                                                                                                                                cAGCGACCTGGTCGATCGTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCa                   >  2:2050806/1‑149 (MQ=255)
                                                                                                                                 aGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCaa                  <  1:2995135/149‑1 (MQ=255)
                                                                                                                                 aGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCaa                  >  1:928494/1‑149 (MQ=255)
                                                                                                                                   cGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAAc                <  2:1352360/149‑1 (MQ=255)
                                                                                                                                    gACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACt               <  1:552963/149‑1 (MQ=255)
                                                                                                                                     aCCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTg              >  2:1478366/1‑149 (MQ=255)
                                                                                                                                        tGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGtt           >  2:2811348/1‑149 (MQ=255)
                                                                                                                                         ggTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTa          <  2:1579488/149‑1 (MQ=255)
                                                                                                                                         ggTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTa          <  1:1426637/149‑1 (MQ=255)
                                                                                                                                           tCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACg        >  2:1956065/1‑149 (MQ=255)
                                                                                                                                            cGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGc       <  1:25702/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCTGCAGATTATGCAGAACGTCTGGCGGCACTGACGCAAAAATGTGGCCTTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGC  >  CP009273/1336454‑1336747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑