Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,341,886 T→A 16.1% A428A (GCA→GCT)  rnb ← ribonuclease II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,341,8860TA16.1% 696.3 / 114.8 260A428A (GCA→GCT) rnbribonuclease II
Reads supporting (aligned to +/- strand):  ref base T (106/112);  new base A (22/20);  total (128/132)
Fisher's exact test for biased strand distribution p-value = 7.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 263 total aligned reads displayed.

TAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTCGG  >  CP009273/1341738‑1342034
                                                                                                                                                    |                                                                                                                                                    
tAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCt                                                                                                                                                      <  2:383957/149‑1 (MQ=255)
   tttCAGCAAATGACTGGAAGCGACGACTGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGcc                                                                                                                                                   <  2:3485950/149‑1 (MQ=255)
   tttCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGcc                                                                                                                                                   <  2:943502/149‑1 (MQ=255)
   tttCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGcc                                                                                                                                                   <  1:1202372/149‑1 (MQ=255)
   tttCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGcc                                                                                                                                                   >  2:888961/1‑149 (MQ=255)
   tttCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGcc                                                                                                                                                   <  1:3026528/149‑1 (MQ=255)
     tCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGACCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAg                                                                                                                                                 >  1:393092/1‑149 (MQ=255)
      cAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAgc                                                                                                                                                <  1:1953463/149‑1 (MQ=255)
       aGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAgcg                                                                                                                                               >  1:1551667/1‑149 (MQ=255)
        gCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCAGTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAgcgc                                                                                                                                              >  1:3671692/1‑149 (MQ=255)
        gCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAgcgc                                                                                                                                              >  2:830153/1‑149 (MQ=255)
        gCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAgcgc                                                                                                                                              >  2:125653/1‑149 (MQ=255)
        gCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAgcgc                                                                                                                                              >  2:2200521/1‑149 (MQ=255)
            aTGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCg                                                                                                                                          >  2:474952/1‑149 (MQ=255)
             tGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCgg                                                                                                                                         >  1:499612/1‑149 (MQ=255)
             tGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCgg                                                                                                                                         <  2:3403417/149‑1 (MQ=255)
                cTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCAt                                                                                                                                      >  1:662628/1‑149 (MQ=255)
                 tGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCAtt                                                                                                                                     >  1:466231/1‑149 (MQ=255)
                        gacgaATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGa                                                                                                                              <  1:3379534/149‑1 (MQ=255)
                        gacgaATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGa                                                                                                                              <  1:1409949/149‑1 (MQ=255)
                          cgaATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATc                                                                                                                            >  2:1059163/1‑149 (MQ=255)
                             aTGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCaaa                                                                                                                         >  1:1712031/1‑149 (MQ=255)
                             aTGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCaaa                                                                                                                         <  2:2190392/149‑1 (MQ=255)
                                  gCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCa                                                                                                                    <  2:399562/149‑1 (MQ=255)
                                  gCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCa                                                                                                                    <  2:403290/149‑1 (MQ=255)
                                    tGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCata                                                                                                                  <  2:3492894/149‑1 (MQ=255)
                                    tGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCata                                                                                                                  <  2:1560462/149‑1 (MQ=255)
                                      tCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATg                                                                                                                >  1:198633/1‑149 (MQ=255)
                                        gAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCa                                                                                                              >  1:859986/1‑149 (MQ=255)
                                        gAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCa                                                                                                              <  1:1938374/149‑1 (MQ=255)
                                         aGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCAc                                                                                                             >  1:1150422/1‑149 (MQ=255)
                                         aGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCAc                                                                                                             >  2:1526759/1‑149 (MQ=255)
                                             aaCCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTta                                                                                                         <  1:1449967/149‑1 (MQ=255)
                                               ccAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTtata                                                                                                       <  1:3396625/149‑1 (MQ=255)
                                                 aGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGa                                                                                                     >  2:332373/1‑149 (MQ=255)
                                                    tggttgCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGc                                                                                                  <  1:3076542/149‑1 (MQ=255)
                                                     ggttgCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGcc                                                                                                 <  1:2834836/149‑1 (MQ=255)
                                                         gcgcGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCaaaa                                                                                             >  1:1387148/1‑149 (MQ=255)
                                                          cgcgTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAAc                                                                                            <  2:2680135/149‑1 (MQ=255)
                                                          cgcgTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAAc                                                                                            <  1:2330485/149‑1 (MQ=255)
                                                           gcgTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAAcc                                                                                           >  1:3332258/1‑149 (MQ=255)
                                                            cgTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCGTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCg                                                                                          <  2:1292492/149‑1 (MQ=255)
                                                              tCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAg                                                                                        <  1:2727305/149‑1 (MQ=255)
                                                              tCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAg                                                                                        >  2:3610252/1‑149 (MQ=255)
                                                                cAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCt                                                                                      <  2:2376723/149‑1 (MQ=255)
                                                                cAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCt                                                                                      <  2:1719066/149‑1 (MQ=255)
                                                                cAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGAATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCt                                                                                      <  1:2847902/149‑1 (MQ=255)
                                                                      acgacgCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATcgc                                                                                <  2:2333735/149‑1 (MQ=255)
                                                                      acgacgCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATcgc                                                                                >  2:66002/1‑149 (MQ=255)
                                                                         acgCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCa                                                                             >  1:184655/1‑149 (MQ=255)
                                                                         acgCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCa                                                                             >  1:184622/1‑149 (MQ=255)
                                                                               tttGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTAcgc                                                                       <  1:378603/149‑1 (MQ=255)
                                                                                ttGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTAcgcg                                                                      <  1:2914350/149‑1 (MQ=255)
                                                                                     gAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCg                                                                 >  2:4086251/1‑149 (MQ=255)
                                                                                       aaCCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGca                                                               >  1:1021036/1‑149 (MQ=255)
                                                                                        aCCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGcac                                                              >  1:2884159/1‑149 (MQ=255)
                                                                                           gTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACaaa                                                           >  2:3072909/1‑149 (MQ=255)
                                                                                            tCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAAt                                                          <  1:2675717/149‑1 (MQ=255)
                                                                                             ccAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATg                                                         <  2:393092/149‑1 (MQ=255)
                                                                                              cAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGt                                                        <  1:605347/149‑1 (MQ=255)
                                                                                                gCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTa                                                      <  2:1679750/149‑1 (MQ=255)
                                                                                                gCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTa                                                      >  1:4022635/1‑149 (MQ=255)
                                                                                                 cGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAg                                                     <  2:2002342/149‑1 (MQ=255)
                                                                                                  gTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGc                                                    >  2:1073476/1‑149 (MQ=255)
                                                                                                   tGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCg                                                   >  1:2731510/1‑149 (MQ=255)
                                                                                                    gAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCgg                                                  >  2:1932597/1‑149 (MQ=255)
                                                                                                       cACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGTATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCaa                                               <  1:3916792/149‑1 (MQ=255)
                                                                                                       cACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCaa                                               <  1:1309328/149‑1 (MQ=255)
                                                                                                       cACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCaa                                               <  1:2570589/149‑1 (MQ=255)
                                                                                                       cACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCaa                                               <  1:1993033/149‑1 (MQ=255)
                                                                                                         cttcttCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAatc                                             >  1:1049654/1‑149 (MQ=255)
                                                                                                         cttcttCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAatc                                             >  2:3706838/1‑149 (MQ=255)
                                                                                                           tcttcGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAatcat                                           <  1:1795991/149‑1 (MQ=255)
                                                                                                           tcttcGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAatcat                                           >  1:1342755/1‑149 (MQ=255)
                                                                                                            cttcGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAatcatc                                          >  2:2879889/1‑149 (MQ=255)
                                                                                                              tcGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGc                                        <  2:2955545/149‑1 (MQ=255)
                                                                                                                ggCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGctt                                      >  1:535418/1‑149 (MQ=255)
                                                                                                                ggCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGctt                                      >  2:3256008/1‑149 (MQ=255)
                                                                                                                ggCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGctt                                      >  2:2831688/1‑149 (MQ=255)
                                                                                                                ggCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGctt                                      >  2:2831970/1‑149 (MQ=255)
                                                                                                                 gCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGcttc                                     <  2:2083542/149‑1 (MQ=255)
                                                                                                                 gCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGcttc                                     <  1:2412692/149‑1 (MQ=255)
                                                                                                                 gCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGcttc                                     >  2:1156333/1‑149 (MQ=255)
                                                                                                                  cATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGcttct                                    <  1:2065178/149‑1 (MQ=255)
                                                                                                                      gACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTcga                                >  2:2766120/1‑149 (MQ=255)
                                                                                                                       aCATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTcgac                               <  2:3158492/149‑1 (MQ=255)
                                                                                                                       aCATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTcgac                               <  1:1619664/149‑1 (MQ=255)
                                                                                                                          tGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGAt                            >  2:1346172/1‑149 (MQ=255)
                                                                                                                          tGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGAt                            >  1:3887638/1‑149 (MQ=255)
                                                                                                                               aCCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGt                       >  1:2454702/1‑149 (MQ=255)
                                                                                                                                ccGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGtt                      <  1:2146531/149‑1 (MQ=255)
                                                                                                                                 cGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTg                     <  1:3732159/149‑1 (MQ=255)
                                                                                                                                  gTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTgg                    <  1:888961/149‑1 (MQ=255)
                                                                                                                                   tGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGc                   <  2:1510837/149‑1 (MQ=255)
                                                                                                                                        tttCAGCAACTCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGc              <  1:2265415/149‑1 (MQ=255)
                                                                                                                                          tCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGcga            <  1:1105764/149‑1 (MQ=255)
                                                                                                                                           cAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGcgac           <  1:2784048/149‑1 (MQ=255)
                                                                                                                                           cAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGcgac           >  1:686359/1‑149 (MQ=255)
                                                                                                                                           cAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGcgac           <  1:3611962/149‑1 (MQ=255)
                                                                                                                                                  gCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTc    <  2:390237/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TAATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTCGG  >  CP009273/1341738‑1342034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑