Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,358,855 G→T 100% G123C (GGC→TGC)  puuB → gamma‑glutamylputrescine oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,358,8550GT98.4% 814.5 / 2.9 254G123C (GGC→TGC) puuBgamma‑glutamylputrescine oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (132/118);  total (134/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 257 total aligned reads displayed.

TGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCG  >  CP009273/1358709‑1358999
                                                                                                                                                  |                                                                                                                                                
   aCTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCtgcggc                                                                                                                                             <  1:80626/149‑1 (MQ=255)
    cTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGcc                                                                                                                                            <  1:3955856/149‑1 (MQ=255)
     tCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCt                                                                                                                                           >  1:2477731/1‑149 (MQ=255)
      ccTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTg                                                                                                                                          <  1:1114032/149‑1 (MQ=255)
        tACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGtt                                                                                                                                        <  1:3718736/149‑1 (MQ=255)
          cAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAATCGTTCTCAGATTGACTGCGACTACCGCCCCTGCGGCCTGGTtg                                                                                                                                      >  2:3074547/1‑149 (MQ=255)
          cAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTtg                                                                                                                                      >  2:625244/1‑149 (MQ=255)
          cAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTtg                                                                                                                                      >  1:497063/1‑149 (MQ=255)
            gCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTtgtg                                                                                                                                    <  2:3130201/149‑1 (MQ=255)
                cgACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGa                                                                                                                                <  2:2675221/149‑1 (MQ=255)
                cgACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGa                                                                                                                                <  2:611200/149‑1 (MQ=255)
                    aTCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatgaa                                                                                                                            <  2:3612043/149‑1 (MQ=255)
                    aTCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGatgaa                                                                                                                            >  2:203866/1‑149 (MQ=255)
                     tCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatgaat                                                                                                                           <  2:2564832/149‑1 (MQ=255)
                        aCGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGAt                                                                                                                        >  2:2786762/1‑149 (MQ=255)
                         cGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATa                                                                                                                       >  2:44694/1‑149 (MQ=255)
                           tGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATaaa                                                                                                                     >  1:2711141/1‑149 (MQ=255)
                            gATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAAc                                                                                                                    <  2:3408665/149‑1 (MQ=255)
                             aTCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACa                                                                                                                   >  2:1537683/1‑149 (MQ=255)
                             aTCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACa                                                                                                                   >  1:3738068/1‑149 (MQ=255)
                             aTCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACa                                                                                                                   >  1:3698554/1‑149 (MQ=255)
                              tCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAg                                                                                                                  <  1:2742056/149‑1 (MQ=255)
                              tCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAg                                                                                                                  >  2:966256/1‑149 (MQ=255)
                                gAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCt                                                                                                                >  1:3101892/1‑149 (MQ=255)
                                  aaaaaGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCg                                                                                                              >  2:344466/1‑149 (MQ=255)
                                  aaaaaGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCg                                                                                                              >  2:1113641/1‑149 (MQ=255)
                                   aaaaGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGc                                                                                                             >  1:1258554/1‑149 (MQ=255)
                                       gCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCcaca                                                                                                         >  2:22837/1‑149 (MQ=255)
                                        cTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCacac                                                                                                        <  2:1717317/149‑1 (MQ=255)
                                          aCGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACtt                                                                                                      <  1:1613663/149‑1 (MQ=255)
                                          aCGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACtt                                                                                                      <  1:1170621/149‑1 (MQ=255)
                                           cGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTg                                                                                                     <  1:1830619/149‑1 (MQ=255)
                                            ggCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGa                                                                                                    >  1:4096590/1‑149 (MQ=255)
                                             gCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGaa                                                                                                   >  1:1524317/1‑149 (MQ=255)
                                               aTGTACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATAGACTGCGACTACCGCCCCTGCGGCCTGTTTCTGGCGATGAATGATAAACAGCTCTCCACACTTGAaga                                                                                                 >  1:2263259/1‑149 (MQ=255)
                                               aTGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAaga                                                                                                 >  2:1955788/1‑149 (MQ=255)
                                               aTGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAaga                                                                                                 >  2:3917393/1‑149 (MQ=255)
                                                 ggACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGc                                                                                               <  2:3587460/149‑1 (MQ=255)
                                                 ggACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGc                                                                                               >  1:2914017/1‑149 (MQ=255)
                                                  gACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCa                                                                                              >  1:221469/1‑149 (MQ=255)
                                                  gACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCa                                                                                              >  2:3725442/1‑149 (MQ=255)
                                                    cacCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGa                                                                                            <  2:313691/149‑1 (MQ=255)
                                                      ccGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGaaa                                                                                          <  2:1004210/149‑1 (MQ=255)
                                                      ccGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGaaa                                                                                          >  1:1096113/1‑149 (MQ=255)
                                                       cGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAag                                                                                         <  1:3673311/149‑1 (MQ=255)
                                                           cGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGaa                                                                                     <  1:1939068/149‑1 (MQ=255)
                                                             tATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACt                                                                                   <  2:1452312/149‑1 (MQ=255)
                                                              aTGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTg                                                                                  >  1:1028718/1‑149 (MQ=255)
                                                                  tCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGaa                                                                              <  1:2368311/149‑1 (MQ=255)
                                                                  tCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGaa                                                                              <  2:3269371/149‑1 (MQ=255)
                                                                    ggCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACg                                                                            >  2:251679/1‑149 (MQ=255)
                                                                       agcaTGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTa                                                                         >  2:1137070/1‑149 (MQ=255)
                                                                        gcaTGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTAc                                                                        >  1:2596696/1‑149 (MQ=255)
                                                                           tgatgTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGc                                                                     >  1:792816/1‑149 (MQ=255)
                                                                               gTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATa                                                                 >  1:695713/1‑149 (MQ=255)
                                                                                ttCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATCAACAGCTCTCCACACTTGAAGAGCAGAAAGAGAACTTGGAACGCTACGTCAATaa                                                                >  2:2920332/1‑149 (MQ=255)
                                                                                  cGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAAc                                                              <  1:2786762/149‑1 (MQ=255)
                                                                                  cGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAAc                                                              <  1:3372228/149‑1 (MQ=255)
                                                                                   gAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTgg                                                                                 >  1:384185/1‑129 (MQ=255)
                                                                                   gAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTgg                                                                                 <  2:384202/129‑1 (MQ=255)
                                                                                       gtggtgAGATCATCCGCGAACGTATCAAACGTTATCAGGTTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTg                                                         >  2:465188/1‑149 (MQ=255)
                                                                                        tggtgAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTgg                                                        <  1:1195972/149‑1 (MQ=255)
                                                                                          gtgAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGaa                                                      <  2:3039521/149‑1 (MQ=255)
                                                                                          gtgAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGaa                                                      <  1:2284778/149‑1 (MQ=255)
                                                                                          gtgAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGaa                                                      >  1:2646869/1‑149 (MQ=255)
                                                                                           tgAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAAt                                                     <  2:787611/149‑1 (MQ=255)
                                                                                           tgAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAAt                                                     <  1:3625678/149‑1 (MQ=255)
                                                                                            gagaTCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAAtt                                                    >  1:3314019/1‑149 (MQ=255)
                                                                                              gaTCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGc                                                  <  1:3236174/149‑1 (MQ=255)
                                                                                               atcatcCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCt                                                 >  1:1792765/1‑149 (MQ=255)
                                                                                                  atcCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGa                                              >  2:1294857/1‑149 (MQ=255)
                                                                                                   tcCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGac                                             <  1:1273412/149‑1 (MQ=255)
                                                                                                     cgcgAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgc                                           <  1:4055041/149‑1 (MQ=255)
                                                                                                       cgAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgcca                                         <  1:3024039/149‑1 (MQ=255)
                                                                                                       cgAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgcca                                         <  1:136182/149‑1 (MQ=255)
                                                                                                       cgAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgcca                                         <  2:1157050/149‑1 (MQ=255)
                                                                                                        gAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgccaa                                        <  2:3744449/149‑1 (MQ=255)
                                                                                                          aCGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgccaacg                                      >  1:3482818/1‑149 (MQ=255)
                                                                                                            gTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgccaacgcc                                    >  1:2950831/1‑149 (MQ=255)
                                                                                                             tATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgccaacgcca                                   <  2:723143/149‑1 (MQ=255)
                                                                                                             tATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgccaacgcca                                   <  2:2026224/149‑1 (MQ=255)
                                                                                                              aTCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCAt                                  <  2:1238312/149‑1 (MQ=255)
                                                                                                               tCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCAtt                                 >  1:370314/1‑149 (MQ=255)
                                                                                                               tCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCAtt                                 >  2:1612046/1‑149 (MQ=255)
                                                                                                                cAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTc                                <  2:2269655/149‑1 (MQ=255)
                                                                                                                cAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTc                                <  2:3793239/149‑1 (MQ=255)
                                                                                                                cAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTc                                <  1:731946/149‑1 (MQ=255)
                                                                                                                cAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTAGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTc                                <  2:194595/149‑1 (MQ=255)
                                                                                                                  aaCGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGc                              >  1:1673594/1‑149 (MQ=255)
                                                                                                                  aaCGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGc                              >  2:209754/1‑149 (MQ=255)
                                                                                                                   aCGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGcc                             >  1:233661/1‑149 (MQ=255)
                                                                                                                    cGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCg                            <  1:3183233/149‑1 (MQ=255)
                                                                                                                     gTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGt                           <  2:1818885/149‑1 (MQ=255)
                                                                                                                           aGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTa                     >  1:1460988/1‑149 (MQ=255)
                                                                                                                            gATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAg                    >  1:1986203/1‑149 (MQ=255)
                                                                                                                              ttGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGcc                  >  1:950309/1‑149 (MQ=255)
                                                                                                                                gACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAg                >  1:1909498/1‑149 (MQ=255)
                                                                                                                                   tGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGa             >  1:1186772/1‑149 (MQ=255)
                                                                                                                                         tACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCta       <  1:161699/149‑1 (MQ=255)
                                                                                                                                            cGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATAc    <  1:3935154/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCG  >  CP009273/1358709‑1358999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑