Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,386,008 G→T 100% Q35K (CAG→AAG)  ycjY ← S9 homolog non‑peptidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,386,0080GT99.5% 676.6 / ‑3.2 212coding (103/921 nt)ycjYS9 homolog non‑peptidase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (77/134);  minor base . (1/0);  total (78/134)
Fisher's exact test for biased strand distribution p-value = 3.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 214 total aligned reads displayed.

AACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGA  >  CP009273/1385863‑1386153
                                                                                                                                                 |                                                                                                                                                 
aaCTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAAAGCCCCCACCTGGGTGAGATAACACAAATGCCTtata                                                                                                                                                >  2:61544/1‑149 (MQ=255)
  cTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCATTAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACt                                                                                                                                              <  2:1598276/149‑1 (MQ=255)
  cTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACt                                                                                                                                              <  2:810841/149‑1 (MQ=255)
  cTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACt                                                                                                                                              <  2:3643454/149‑1 (MQ=255)
  cTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACt                                                                                                                                              <  2:2040245/149‑1 (MQ=255)
   tGACGAGGCTGTCCTCCACTTTAACCTTGATAATATGCGTCATAAGATTTTGTGACAAATCCCTTTTCCGCCAGTTTTTTGTCATAGGTTCCGGCCTTTTGTTATTTTACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTg                                                                                                                                             <  2:4063299/149‑1 (MQ=255)
         ggCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGt                                                                                                                                       <  1:3901580/149‑1 (MQ=255)
         ggCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGGTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCGTATACTGACGGGt                                                                                                                                       >  2:201590/1‑149 (MQ=255)
           cTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGttt                                                                                                                                     <  1:789267/149‑1 (MQ=255)
            tCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGGTTAACGCCCCCACCTGGGTGTGATAAAACAATTTCCGTATACTGACGGGGTtc                                                                                                                                    >  2:500109/1‑149 (MQ=255)
             cgccgcCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAAAGCCCCCACCAGGGGGGGATAACAAAAATGCCGTATAAAGACGGGgttga                                                                                                                                   >  1:230119/1‑147 (MQ=255)
               ccgccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTtcatc                                                                                                                                 <  1:3413749/149‑1 (MQ=255)
                  ccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCaaa                                                                                                                              <  2:2544088/149‑1 (MQ=255)
                  ccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCaaa                                                                                                                              <  2:187088/149‑1 (MQ=255)
                     cTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAttt                                                                                                                           <  1:46301/149‑1 (MQ=255)
                      tttCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAAAACAATTGGCTTATAATGACGGGTTTCATCAAAtttt                                                                                                                          >  2:2407012/1‑149 (MQ=255)
                      tttCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAAAAAAAATGCCTTATACTTACGGGTTTCATCAAAtttt                                                                                                                          >  2:3166276/1‑149 (MQ=255)
                      tttCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATAATGACGGGTTTCATCAAAtttt                                                                                                                          >  2:3940781/1‑149 (MQ=255)
                       ttCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCATATAATGACGGGTTTAAAAAAATTTTg                                                                                                                         >  2:345152/1‑149 (MQ=255)
                       ttCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTg                                                                                                                         <  1:2679603/149‑1 (MQ=255)
                         cACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGa                                                                                                                       <  1:2037762/149‑1 (MQ=255)
                         cACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAAGCCCCCACCGGGGGGAGATAACACAATTGCCTTATACTGACGGGTTTGATCAAAATTTggt                                                                                                                       >  1:3660009/1‑148 (MQ=255)
                         cACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAACGCCCCCCCCGGGGTGAGATAACAAAAATTCCTTATACTGACGGGGTTCATCAAAATTTTGa                                                                                                                       >  2:1873583/1‑149 (MQ=255)
                               gATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGaaa                                                                                                                 <  1:3701896/149‑1 (MQ=255)
                                aTAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAAt                                                                                                                <  1:4086587/149‑1 (MQ=255)
                                aTAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAAt                                                                                                                <  1:220219/149‑1 (MQ=255)
                                 tAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATa                                                                                                               <  1:489262/149‑1 (MQ=255)
                                   aGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTATTTAAAGCCCCCACCTGGGTGTGATAACACAAATTCCTTATACTGAGGGGTTTGATAAAATTTTTGAGGGAAATata                                                                                                             >  2:1174155/1‑147 (MQ=255)
                                    gATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAAAGCCCCCCCCTGGGTGAGTTAACACAAATGCCGTATACTGACGGGTTTCATCAAAGTTTGGAGGGAAATAGAt                                                                                                            >  2:2582619/1‑149 (MQ=255)
                                     aTGCGTCATAAGCAATAGTGAAAAATCCCTTTTCCGACAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGATATAACACAATTTCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATc                                                                                                           <  2:2209529/149‑1 (MQ=255)
                                      tGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCa                                                                                                          <  1:811565/149‑1 (MQ=255)
                                      tGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAACGCCCCCCCCGGGGGGGGATAACACAAATGCCTTATACAGACGGGTTTAATCAAAATTTGGAGGGAAATATATCa                                                                                                          >  1:2372432/1‑149 (MQ=255)
                                      tGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAAAGCCCCCACCTGGGTGAGATAACACAATTGCCGTATACTGACGGGTTTCATCAAATTTTTGAGGGAAATAGAtaa                                                                                                          >  2:2969731/1‑147 (MQ=255)
                                       gCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCAc                                                                                                         <  2:2750911/149‑1 (MQ=255)
                                          tCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGc                                                                                                      <  1:61544/149‑1 (MQ=255)
                                          tCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCGGGGTGCGTTAACACCATTGGCTTATACTGACGGGGTTCATCCAATTTTGGCGGGAAATAGATCaaggc                                                                                                      >  2:3320392/1‑145 (MQ=255)
                                          tCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTGGTTGTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTAATCAAATTTTGGAGGGAAATAGATCACTGc                                                                                                      >  2:1362530/1‑149 (MQ=255)
                                           cATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCa                                                                                                     <  1:2992802/149‑1 (MQ=255)
                                               aGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTTAAGCCCCCCCCCGGGTGTGATAACACAAATTCCGTATAATGAAGGGGTTAATGAAAATTTTGTGGGTAAAAGATAACTTCAGaaa                                                                                                 >  1:560430/1‑147 (MQ=255)
                                                 cAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACaaa                                                                                               <  1:1911714/149‑1 (MQ=255)
                                                   aTAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCAGGGTGAGATAACACAAATTCCGTATACTGTCGGGTTTCATCAAATTTTGGAGGGAAATAGATAACAGCAGACAAaga                                                                                             >  2:3221733/1‑149 (MQ=255)
                                                         aCAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGt                                                                                       <  1:3171982/149‑1 (MQ=255)
                                                               cccTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCGGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTGATGAAAATTTTGAGGGTAATATATAACTGCAGACAAAGAGATGGTTGGgtt                                                                                 >  2:3614343/1‑149 (MQ=255)
                                                                ccTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGatta                                                                                <  2:3436658/149‑1 (MQ=255)
                                                                 cTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGattat                                                                               <  2:1756067/149‑1 (MQ=255)
                                                                 cTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAAATGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGGAATAGATAACTGCAGACAAAGAGATGGGTGGtttat                                                                               >  2:638967/1‑149 (MQ=255)
                                                                 cTTTTCCGCCAGTTTTTGGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGattat                                                                               <  1:3857190/149‑1 (MQ=255)
                                                                 cTTGTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGattat                                                                               <  2:2490084/149‑1 (MQ=255)
                                                                       cGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAACGCCCCCACCAGGGGGTGATTACACAAATTCCATATACTGACGGGTTTGATCAAAATTTTGTGGGTAATAGATAAAAGCAGACAAACAGTTGGTTGGATTTTTTCTGt                                                                         >  1:3014033/1‑149 (MQ=255)
                                                                        gCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGtt                                                                        <  1:3817653/149‑1 (MQ=255)
                                                                         ccAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTa                                                                       <  2:722201/149‑1 (MQ=255)
                                                                         ccAGTTTTTTGGCATAGGGTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTa                                                                       <  1:3824909/149‑1 (MQ=255)
                                                                             ttttttGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGa                                                                   <  2:2376319/149‑1 (MQ=255)
                                                                                 ttGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCt                                                               <  1:3119512/149‑1 (MQ=255)
                                                                                 ttGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCt                                                               <  2:2254567/149‑1 (MQ=255)
                                                                                   ggCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGa                                                             <  1:576530/149‑1 (MQ=255)
                                                                                   ggCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGa                                                             <  2:2806429/149‑1 (MQ=255)
                                                                                       tAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACttt                                                         <  1:1322138/149‑1 (MQ=255)
                                                                                            tCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTtgttat                                                    <  1:349605/149‑1 (MQ=255)
                                                                                            tCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTtattat                                                    <  1:349560/149‑1 (MQ=255)
                                                                                             ccGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTattatt                                                   <  2:2172363/149‑1 (MQ=255)
                                                                                             ccGGCCGTTTGTTCTTTAAAGCCCCCACCAGGGTGAGATAACAAAATTTCCTTATACTGACGGGTTTCATAAAATTTTGGAGGGAAATATATAACTGCAGAAAAAGAGAAGGTTGGAGTATTGGTGTTATTGAAGCTTGATTTattatt                                                   >  2:4040919/1‑149 (MQ=255)
                                                                                               ggCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtca                                                 <  1:3966936/149‑1 (MQ=255)
                                                                                                   gTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTcatcat                                             >  2:3535587/1‑149 (MQ=255)
                                                                                                   gTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTcatcat                                             <  2:1643369/149‑1 (MQ=255)
                                                                                                   gTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTcatcat                                             >  2:1485060/1‑149 (MQ=255)
                                                                                                     ttGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTc                                           <  1:140543/149‑1 (MQ=255)
                                                                                                     ttGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTc                                           >  2:1580219/1‑149 (MQ=255)
                                                                                                      tGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGTCGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTcc                                          <  1:115526/149‑1 (MQ=255)
                                                                                                      tGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTcc                                          <  2:1934221/149‑1 (MQ=255)
                                                                                                      tGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTcc                                          <  1:115567/149‑1 (MQ=255)
                                                                                                      tGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTcc                                          <  2:306930/149‑1 (MQ=255)
                                                                                                        ttCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGt                                        <  2:1112754/149‑1 (MQ=255)
                                                                                                         tCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGtt                                       <  1:2222857/149‑1 (MQ=255)
                                                                                                           tttAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTcc                                     <  1:1105157/149‑1 (MQ=255)
                                                                                                           tttAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTcc                                     <  2:2148026/149‑1 (MQ=255)
                                                                                                            ttAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCt                                    >  1:1861079/1‑149 (MQ=255)
                                                                                                            ttAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCt                                    <  1:3106618/149‑1 (MQ=255)
                                                                                                                cGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATg                                <  2:538167/149‑1 (MQ=255)
                                                                                                                 gCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATgg                               >  2:1434243/1‑149 (MQ=255)
                                                                                                                 gCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATgg                               <  1:1819252/149‑1 (MQ=255)
                                                                                                                   ccccACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAg                             <  1:2146832/149‑1 (MQ=255)
                                                                                                                    cccACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGTAGt                            >  1:1549077/1‑149 (MQ=255)
                                                                                                                     ccACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGtt                           >  1:1480182/1‑149 (MQ=255)
                                                                                                                       aCCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGt                         >  1:4014473/1‑149 (MQ=255)
                                                                                                                        ccTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTc                        <  1:1011939/149‑1 (MQ=255)
                                                                                                                         cTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCg                       >  1:1608931/1‑149 (MQ=255)
                                                                                                                             gTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTc                   >  2:1090822/1‑149 (MQ=255)
                                                                                                                              tGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCg                  >  1:1552026/1‑149 (MQ=255)
                                                                                                                                agaTAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGtt                <  1:2852342/149‑1 (MQ=255)
                                                                                                                                 gaTAACACAATTGCCTTATACTGACGGGTTTCATCCAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGttt               >  1:605734/1‑149 (MQ=255)
                                                                                                                                  aTAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGtttt              <  2:432058/149‑1 (MQ=255)
                                                                                                                                    aaCACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTaa            <  1:1434243/149‑1 (MQ=255)
                                                                                                                                    aaCACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTaa            <  1:4021497/149‑1 (MQ=255)
                                                                                                                                    aaCACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGAACACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTaa            <  1:4021726/149‑1 (MQ=255)
                                                                                                                                     acacAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAAc           <  2:3861410/149‑1 (MQ=255)
                                                                                                                                     acacAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAAc           <  2:2774450/149‑1 (MQ=255)
                                                                                                                                          aTTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTACTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTg      <  1:2472956/149‑1 (MQ=255)
                                                                                                                                            tGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGt    <  1:112215/149‑1 (MQ=255)
                                                                                                                                              ccTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGa  >  1:510577/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGA  >  CP009273/1385863‑1386153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑