Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 10,444 G→T 100% F17L (TTC→TTA)  satP ← succinate‑acetate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927310,4440GT99.5% 721.9 / ‑4.3 216F17L (TTC→TTA) satPsuccinate‑acetate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (93/122);  total (93/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 216 total aligned reads displayed.

AAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGG  >  CP009273/10298‑10589
                                                                                                                                                  |                                                                                                                                                 
aaaCCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTaa                                                                                                                                                 >  2:2012076/1‑149 (MQ=255)
 aaCCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAg                                                                                                                                                <  1:939960/149‑1 (MQ=255)
  aCCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGc                                                                                                                                               <  1:3066497/149‑1 (MQ=255)
  aCCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCANGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGc                                                                                                                                               <  1:2085444/149‑1 (MQ=255)
   ccGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGcc                                                                                                                                              <  2:3005353/149‑1 (MQ=255)
     gAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCa                                                                                                                                            >  1:2024782/1‑149 (MQ=255)
      aaaGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCAt                                                                                                                                           >  1:1258019/1‑149 (MQ=255)
      aaaGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCAt                                                                                                                                           >  2:2050802/1‑149 (MQ=255)
         gtgtTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAg                                                                                                                                        <  1:2967309/149‑1 (MQ=255)
          tgtTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAgg                                                                                                                                       >  1:1036218/1‑149 (MQ=255)
             tGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGccc                                                                                                                                    >  2:2100048/1‑149 (MQ=255)
             tGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGccc                                                                                                                                    <  1:2599587/149‑1 (MQ=255)
                cTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGc                                                                                                                                 <  1:3549393/149‑1 (MQ=255)
                     ttGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGc                                                                                                                            <  1:3206494/149‑1 (MQ=255)
                      tGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGcc                                                                                                                           <  2:2132780/149‑1 (MQ=255)
                       gTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCg                                                                                                                          >  2:2398622/1‑149 (MQ=255)
                          cTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGAt                                                                                                                       >  1:3765686/1‑149 (MQ=255)
                           tCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGAtt                                                                                                                      >  2:1014758/1‑149 (MQ=255)
                              agcagACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGc                                                                                                                   >  2:1665059/1‑149 (MQ=255)
                              agcagACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGc                                                                                                                   >  2:3709838/1‑149 (MQ=255)
                              agcagACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGc                                                                                                                   >  1:4114235/1‑149 (MQ=255)
                                 agACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCaa                                                                                                                >  1:1583105/1‑149 (MQ=255)
                                   aCCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATACTACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACt                                                                                                              <  2:1174949/149‑1 (MQ=255)
                                   aCCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACt                                                                                                              >  1:2767508/1‑149 (MQ=255)
                                    ccAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACtt                                                                                                             <  2:912268/149‑1 (MQ=255)
                                    ccAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACtt                                                                                                             <  2:731444/149‑1 (MQ=255)
                                    ccAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACtt                                                                                                             <  2:1993608/149‑1 (MQ=255)
                                     cAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTa                                                                                                            >  2:996536/1‑149 (MQ=255)
                                     cAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTa                                                                                                            <  2:1546234/149‑1 (MQ=255)
                                      aGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAg                                                                                                           >  1:3604202/1‑149 (MQ=255)
                                      aGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCAACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAg                                                                                                           <  1:834130/149‑1 (MQ=255)
                                       gCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAgt                                                                                                          >  2:2638647/1‑149 (MQ=255)
                                        cAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAgtg                                                                                                         <  1:2117158/149‑1 (MQ=255)
                                        cAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAgtg                                                                                                         <  1:38862/149‑1 (MQ=255)
                                          aaaaTTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGtt                                                                                                       >  2:50335/1‑149 (MQ=255)
                                          aaaaTTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGtt                                                                                                       >  2:3099174/1‑149 (MQ=255)
                                               ttGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCa                                                                                                  <  2:3907328/149‑1 (MQ=255)
                                                 gcgcGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATa                                                                                                <  2:1757878/149‑1 (MQ=255)
                                                 gcgcGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATa                                                                                                >  2:2817291/1‑149 (MQ=255)
                                                    cgATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAAtt                                                                                             <  1:666106/149‑1 (MQ=255)
                                                      aTGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTcc                                                                                           >  1:820370/1‑149 (MQ=255)
                                                        gccgccGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTc                                                                                         <  1:2174378/149‑1 (MQ=255)
                                                             cgTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCaaaaa                                                                                    <  1:4037373/149‑1 (MQ=255)
                                                                aGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatca                                                                                 >  1:906374/1‑149 (MQ=255)
                                                                 gAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcat                                                                                >  1:2539827/1‑149 (MQ=255)
                                                                  aaaaTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatc                                                                               <  1:4109245/149‑1 (MQ=255)
                                                                  aaaaTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatc                                                                               <  1:3940694/149‑1 (MQ=255)
                                                                   aaaTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatca                                                                              <  2:2475775/149‑1 (MQ=255)
                                                                   aaaTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatca                                                                              <  2:3154919/149‑1 (MQ=255)
                                                                    aaTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatcat                                                                             >  1:1544064/1‑149 (MQ=255)
                                                                    aaTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatcat                                                                             >  2:1640843/1‑149 (MQ=255)
                                                                    aaTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatcat                                                                             <  1:2704074/149‑1 (MQ=255)
                                                                     aTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatcatc                                                                            >  2:3818285/1‑149 (MQ=255)
                                                                        cccATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaa                                                                         <  1:3496949/149‑1 (MQ=255)
                                                                        cccATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaa                                                                         >  1:704043/1‑149 (MQ=255)
                                                                           aTGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaatga                                                                      <  2:3516677/149‑1 (MQ=255)
                                                                               cAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATgg                                                                  >  2:3757543/1‑149 (MQ=255)
                                                                                aaGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGt                                                                 <  2:3692034/149‑1 (MQ=255)
                                                                                   aataatACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGaa                                                              >  2:3615196/1‑149 (MQ=255)
                                                                                    ataataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGaaa                                                             <  2:534239/149‑1 (MQ=255)
                                                                                    ataataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGaaa                                                             <  2:536606/149‑1 (MQ=255)
                                                                                     taataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAaat                                                            <  1:2398622/149‑1 (MQ=255)
                                                                                      aataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAaata                                                           >  2:3743381/1‑149 (MQ=255)
                                                                                       ataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAaataa                                                          >  1:374691/1‑149 (MQ=255)
                                                                                          ccGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAAttt                                                       <  1:3031636/149‑1 (MQ=255)
                                                                                            gTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTcc                                                     <  1:3593645/149‑1 (MQ=255)
                                                                                               cAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTg                                                  >  2:4014226/1‑149 (MQ=255)
                                                                                                agagCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGa                                                 <  2:586447/149‑1 (MQ=255)
                                                                                                 gagCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGaa                                                <  2:2616613/149‑1 (MQ=255)
                                                                                                  agCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAAt                                               <  1:2817291/149‑1 (MQ=255)
                                                                                                    cGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATaa                                             <  1:2858494/149‑1 (MQ=255)
                                                                                                     gAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAAc                                            <  2:2445687/149‑1 (MQ=255)
                                                                                                     gAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAAc                                            <  2:2969549/149‑1 (MQ=255)
                                                                                                         tAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTa                                        >  2:2213311/1‑149 (MQ=255)
                                                                                                         tAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTa                                        >  2:135526/1‑149 (MQ=255)
                                                                                                          aaCCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAg                                       <  1:1848497/149‑1 (MQ=255)
                                                                                                             ccACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAgtgt                                    <  2:3780859/149‑1 (MQ=255)
                                                                                                             ccACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAgtgt                                    <  2:3781050/149‑1 (MQ=255)
                                                                                                             ccACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAgtgt                                    <  1:1984278/149‑1 (MQ=255)
                                                                                                                 gTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTc                                <  2:255050/149‑1 (MQ=255)
                                                                                                                   tgtgCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAg                              <  2:1076835/149‑1 (MQ=255)
                                                                                                                     tgCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAggg                            >  2:3369163/1‑149 (MQ=255)
                                                                                                                      gCAGGTTAAGTAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCCTCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGgc                           <  1:1031227/149‑1 (MQ=255)
                                                                                                                      gCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGgc                           <  2:855555/149‑1 (MQ=255)
                                                                                                                        aGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGgcgc                         >  1:3881090/1‑149 (MQ=255)
                                                                                                                         ggTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGcgcg                        >  2:704878/1‑149 (MQ=255)
                                                                                                                          gTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATACTTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCgg                       >  2:2869118/1‑149 (MQ=255)
                                                                                                                           ttAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGc                      >  2:2332274/1‑149 (MQ=255)
                                                                                                                            tAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCa                     >  2:1609632/1‑149 (MQ=255)
                                                                                                                             aaGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCat                    <  1:1714398/149‑1 (MQ=255)
                                                                                                                              aGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCata                   <  1:3326334/149‑1 (MQ=255)
                                                                                                                               gCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCataa                  <  1:3718957/149‑1 (MQ=255)
                                                                                                                                 aGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCataata                <  2:2073010/149‑1 (MQ=255)
                                                                                                                                   aaTGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATaataat              >  1:2541015/1‑149 (MQ=255)
                                                                                                                                   aaTGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATaataat              <  1:2350993/149‑1 (MQ=255)
                                                                                                                                     tggtggTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCCTCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCa            <  2:2767508/149‑1 (MQ=255)
                                                                                                                                     tggtggTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCa            >  1:1889356/1‑149 (MQ=255)
                                                                                                                                           tCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGt      >  1:2228936/1‑149 (MQ=255)
                                                                                                                                            cATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTg     <  1:3750687/149‑1 (MQ=255)
                                                                                                                                               gCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTgggg  <  2:2848006/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGG  >  CP009273/10298‑10589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑