Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 119,536 G→T 100% P11P (CCG→CCT)  aceE → pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273119,5360GT98.1% 807.4 / 5.3 259P11P (CCG→CCT) aceEpyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base G (4/1);  new base T (128/126);  total (132/127)
Fisher's exact test for biased strand distribution p-value = 3.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 259 total aligned reads displayed.

CATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCAAC  >  CP009273/119390‑119680
                                                                                                                                                  |                                                                                                                                                
cATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTAt                                                                                                                                                >  2:1384673/1‑149 (MQ=255)
cATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTAt                                                                                                                                                <  1:1343164/149‑1 (MQ=255)
 atatAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATc                                                                                                                                               <  1:1061857/149‑1 (MQ=255)
   ataAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGa                                                                                                                                             >  1:3998929/1‑149 (MQ=255)
    taAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGaa                                                                                                                                            >  1:737427/1‑149 (MQ=255)
      aaaGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAAc                                                                                                                                          <  2:3321051/149‑1 (MQ=255)
      aaaGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAAc                                                                                                                                          <  2:2151090/149‑1 (MQ=255)
                 aCTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATACCCCATGTCAGAACGTTTCCCAAATGACGAGGATCCTATCGAAACTCGCGACTGGc                                                                                                                               >  1:956881/1‑149 (MQ=255)
                 aCTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGc                                                                                                                               >  1:2060464/1‑149 (MQ=255)
                 aCTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGc                                                                                                                               >  2:2081375/1‑149 (MQ=255)
                 aCTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGc                                                                                                                               >  1:4030109/1‑149 (MQ=255)
                 aCTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTACCAAATGACGTGGATCCTATCGAAACTCGCGACTGGc                                                                                                                               >  2:2422786/1‑149 (MQ=255)
                    aaaCGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTcc                                                                                                                            <  2:693908/149‑1 (MQ=255)
                    aaaCGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTcc                                                                                                                            <  2:1826012/149‑1 (MQ=255)
                     aaCGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCa                                                                                                                           >  1:195555/1‑149 (MQ=255)
                     aaCGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCa                                                                                                                           >  2:1698060/1‑149 (MQ=255)
                     aaCGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCa                                                                                                                           >  1:259717/1‑149 (MQ=255)
                     aaCGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCa                                                                                                                           <  2:3959725/149‑1 (MQ=255)
                     aaCGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCa                                                                                                                           >  1:2348767/1‑149 (MQ=255)
                      aCGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAg                                                                                                                          >  2:82543/1‑149 (MQ=255)
                           gAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGAt                                                                                                                     >  1:1718382/1‑149 (MQ=255)
                             aCCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCg                                                                                                                   >  1:151888/1‑149 (MQ=255)
                              ccTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGa                                                                                                                  >  2:733609/1‑149 (MQ=255)
                              ccTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGa                                                                                                                  >  2:1156458/1‑149 (MQ=255)
                              ccTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGa                                                                                                                  >  2:2797687/1‑149 (MQ=255)
                               cTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGGCTGGCTCCAGGCGATCGaa                                                                                                                 <  2:3071716/149‑1 (MQ=255)
                                tGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAAt                                                                                                                <  2:3801386/149‑1 (MQ=255)
                                    ttATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGt                                                                                                            >  2:1100176/1‑149 (MQ=255)
                                     tATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTc                                                                                                           >  2:2540805/1‑149 (MQ=255)
                                     tATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTc                                                                                                           >  2:3542921/1‑149 (MQ=255)
                                     tATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTc                                                                                                           >  2:473030/1‑149 (MQ=255)
                                     tATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTc                                                                                                           >  2:1194915/1‑149 (MQ=255)
                                            cTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTg                                                                                                    >  1:1454424/1‑149 (MQ=255)
                                            cTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTg                                                                                                    >  2:3293027/1‑149 (MQ=255)
                                              ttCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATATATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTgaa                                                                                                  >  2:3942748/1‑149 (MQ=255)
                                              ttCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTgaa                                                                                                  <  2:1277638/149‑1 (MQ=255)
                                                    cGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGgt                                                                                            <  2:1176931/149‑1 (MQ=255)
                                                     gagaGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGgtg                                                                                           <  1:1133167/149‑1 (MQ=255)
                                                      agagTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGgtgt                                                                                          >  1:1873643/1‑149 (MQ=255)
                                                      agagTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGgtgt                                                                                          >  1:690535/1‑149 (MQ=255)
                                                       gagTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGtt                                                                                         <  1:2490897/149‑1 (MQ=255)
                                                         gTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGa                                                                                       <  2:2276218/149‑1 (MQ=255)
                                                          ttCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAg                                                                                      >  1:1155562/1‑149 (MQ=255)
                                                                gggACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCt                                                                                <  1:3005380/149‑1 (MQ=255)
                                                                 ggACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTc                                                                               >  2:1017013/1‑149 (MQ=255)
                                                                  gACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCa                                                                              <  1:3347184/149‑1 (MQ=255)
                                                                     aGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCCTGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTa                                                                           <  2:3575343/149‑1 (MQ=255)
                                                                     aGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTa                                                                           >  2:3754957/1‑149 (MQ=255)
                                                                      ggTTCCAGAAAACTCAACGTTATTAGATAGATACGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTAt                                                                          <  2:3122437/149‑1 (MQ=255)
                                                                      ggTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTAt                                                                          >  2:1333364/1‑149 (MQ=255)
                                                                       gTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATc                                                                         <  1:1426515/149‑1 (MQ=255)
                                                                       gTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATc                                                                         <  1:1642017/149‑1 (MQ=255)
                                                                         tCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTg                                                                       >  1:2488584/1‑149 (MQ=255)
                                                                         tCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTg                                                                       >  1:2184252/1‑149 (MQ=255)
                                                                           cAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGAt                                                                     >  1:3159656/1‑149 (MQ=255)
                                                                             gAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCg                                                                   >  2:3021102/1‑149 (MQ=255)
                                                                                aaCTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGAcc                                                                <  2:96302/149‑1 (MQ=255)
                                                                                 aCTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCa                                                               >  2:118705/1‑149 (MQ=255)
                                                                                 aCTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCa                                                               >  1:3862743/1‑149 (MQ=255)
                                                                                 aCTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGACTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCa                                                               <  2:2939693/149‑1 (MQ=255)
                                                                                   tCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAAc                                                             <  1:3520508/149‑1 (MQ=255)
                                                                                   tCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAAc                                                             <  1:777888/149‑1 (MQ=255)
                                                                                    cAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACt                                                            <  2:2164768/149‑1 (MQ=255)
                                                                                       cGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACtgct                                                         <  1:3191080/149‑1 (MQ=255)
                                                                                        gTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACtgctt                                                        >  1:3592602/1‑149 (MQ=255)
                                                                                          tattaGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACtgcttgc                                                      >  2:1761242/1‑149 (MQ=255)
                                                                                            ttaGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTAGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTg                                                    <  1:3852508/149‑1 (MQ=255)
                                                                                                atagataAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGc                                                <  1:839780/149‑1 (MQ=255)
                                                                                                atagataAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGc                                                >  1:4077970/1‑149 (MQ=255)
                                                                                                  agataAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGccc                                              <  2:3998929/149‑1 (MQ=255)
                                                                                                   gataAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCg                                             >  1:3686876/1‑149 (MQ=255)
                                                                                                       aGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCaaa                                         >  2:2367936/1‑149 (MQ=255)
                                                                                                       aGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCaaa                                         >  1:2394787/1‑149 (MQ=255)
                                                                                                       aGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCaaa                                         <  1:2847906/149‑1 (MQ=255)
                                                                                                       aGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCaaa                                         >  2:3001307/1‑149 (MQ=255)
                                                                                                         gAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAgg                                       >  1:1079073/1‑149 (MQ=255)
                                                                                                              aCCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGgtg                                  >  2:615465/1‑149 (MQ=255)
                                                                                                              aCCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGgtg                                  <  1:3567092/149‑1 (MQ=255)
                                                                                                                     tCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGt                           >  2:2585083/1‑149 (MQ=255)
                                                                                                                       aGAACGTTTCCCACATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAg                         <  2:1088507/149‑1 (MQ=255)
                                                                                                                       aGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAg                         >  2:417204/1‑149 (MQ=255)
                                                                                                                       aGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAg                         >  2:1530701/1‑149 (MQ=255)
                                                                                                                       aGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAg                         <  2:943832/149‑1 (MQ=255)
                                                                                                                        gAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGc                        <  1:3277520/149‑1 (MQ=255)
                                                                                                                        gAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGc                        >  1:569471/1‑149 (MQ=255)
                                                                                                                        gAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGc                        <  1:600247/149‑1 (MQ=255)
                                                                                                                        gAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGc                        >  1:801894/1‑149 (MQ=255)
                                                                                                                         aaCGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGcc                       >  2:3352393/1‑149 (MQ=255)
                                                                                                                         aaCGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGcc                       >  2:1612479/1‑149 (MQ=255)
                                                                                                                             tttCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAg                   >  2:3558276/1‑149 (MQ=255)
                                                                                                                                   aaaTGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAg             <  1:1017002/149‑1 (MQ=255)
                                                                                                                                       gACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTAt         <  2:2702352/149‑1 (MQ=255)
                                                                                                                                        aCGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATc        <  2:1521626/149‑1 (MQ=255)
                                                                                                                                        aCGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTCGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATc        <  1:2235713/149‑1 (MQ=255)
                                                                                                                                         cGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCa       <  2:3417770/149‑1 (MQ=255)
                                                                                                                                           tGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGc     >  2:2023623/1‑149 (MQ=255)
                                                                                                                                             gATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCaa   <  1:2142202/149‑1 (MQ=255)
                                                                                                                                             gATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCaa   >  1:1011555/1‑149 (MQ=255)
                                                                                                                                              aTCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCAAc  >  2:2808450/1‑149 (MQ=255)
                                                                                                                                              aTCCTATAGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCAAc  >  1:1582466/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCAAC  >  CP009273/119390‑119680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑