Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,482,005 G→T 100% D172Y (GAT→TAT)  ydcF → DUF218 superfamily protein, SAM‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,482,0050GT97.8% 868.6 / 8.3 272D172Y (GAT→TAT) ydcFDUF218 superfamily protein, SAM‑binding
Reads supporting (aligned to +/- strand):  ref base G (1/5);  new base T (138/128);  total (139/133)
Fisher's exact test for biased strand distribution p-value = 1.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 272 total aligned reads displayed.

ACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCGATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTACG  >  CP009273/1481861‑1482150
                                                                                                                                                |                                                                                                                                                 
acaaACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGc                                                                                                                                               >  1:2814044/1‑149 (MQ=255)
   aaCTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGcacc                                                                                                                                            >  1:2585591/1‑149 (MQ=255)
    aCTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGcacca                                                                                                                                           <  2:378378/149‑1 (MQ=255)
    aCTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGcacca                                                                                                                                           >  2:3655918/1‑149 (MQ=255)
          gTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTgg                                                                                                                                     >  1:795455/1‑149 (MQ=255)
           tGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGt                                                                                                                                    >  2:3066450/1‑149 (MQ=255)
             aaaaCGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTa                                                                                                                                  >  1:362684/1‑149 (MQ=255)
             aaaaCGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTa                                                                                                                                  >  2:672365/1‑149 (MQ=255)
              aaaCGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTaa                                                                                                                                 <  1:3766107/149‑1 (MQ=255)
              aaaCGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTaa                                                                                                                                 >  1:1881435/1‑149 (MQ=255)
               aaCGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAg                                                                                                                                >  1:339812/1‑149 (MQ=255)
                    acgcTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATc                                                                                                                           >  2:2289940/1‑149 (MQ=255)
                    acgcTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATc                                                                                                                           >  1:1266001/1‑149 (MQ=255)
                      gcTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATccc                                                                                                                         >  2:1643216/1‑149 (MQ=255)
                      gcTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATccc                                                                                                                         <  1:90496/149‑1 (MQ=255)
                       cTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCg                                                                                                                        >  1:590700/1‑149 (MQ=255)
                       cTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCg                                                                                                                        >  2:3921631/1‑149 (MQ=255)
                         ttAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGa                                                                                                                      <  2:817227/149‑1 (MQ=255)
                          tAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGAt                                                                                                                     <  2:2499382/149‑1 (MQ=255)
                          tAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGAt                                                                                                                     <  1:678896/149‑1 (MQ=255)
                           aGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGAtt                                                                                                                    <  2:600466/149‑1 (MQ=255)
                            gCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTc                                                                                                                   <  2:3394952/149‑1 (MQ=255)
                            gCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTc                                                                                                                   <  2:2401706/149‑1 (MQ=255)
                             cATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCg                                                                                                                  <  1:3122363/149‑1 (MQ=255)
                               tCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGtt                                                                                                                >  1:1013443/1‑149 (MQ=255)
                                 gcgcTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTcc                                                                                                              >  1:3862146/1‑149 (MQ=255)
                                   gcTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTc                                                                                                            >  2:2871965/1‑149 (MQ=255)
                                    cTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCa                                                                                                           <  2:2640305/149‑1 (MQ=255)
                                     tATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAg                                                                                                          <  2:2214043/149‑1 (MQ=255)
                                       ttGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGtt                                                                                                        <  1:839213/149‑1 (MQ=255)
                                       ttGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGtt                                                                                                        >  2:3949385/1‑149 (MQ=255)
                                       ttGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGtt                                                                                                        <  1:1667401/149‑1 (MQ=255)
                                            tCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGaa                                                                                                   <  2:1976613/149‑1 (MQ=255)
                                             cAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGaaa                                                                                                  <  2:4093440/149‑1 (MQ=255)
                                              aGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAAt                                                                                                 <  1:1228806/149‑1 (MQ=255)
                                              aGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAAt                                                                                                 >  1:2823520/1‑149 (MQ=255)
                                                gCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATaa                                                                                               <  1:3980996/149‑1 (MQ=255)
                                                gCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATaa                                                                                               >  2:2786787/1‑149 (MQ=255)
                                                gCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATaa                                                                                               <  1:3278907/149‑1 (MQ=255)
                                                 ccGGAGAACGAGTTCATACGGCTATCGTGGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAAc                                                                                              <  1:2472733/149‑1 (MQ=255)
                                                  cGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACg                                                                                             >  2:1770757/1‑149 (MQ=255)
                                                  cGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACg                                                                                             >  1:2883772/1‑149 (MQ=255)
                                                    tAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACCATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCa                                                                                           <  1:3480854/149‑1 (MQ=255)
                                                    tAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCa                                                                                           >  1:2995992/1‑149 (MQ=255)
                                                    tAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCa                                                                                           <  1:377129/149‑1 (MQ=255)
                                                     aGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAg                                                                                          >  1:1794258/1‑149 (MQ=255)
                                                     aGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCGATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAg                                                                                          >  2:720201/1‑149 (MQ=255)
                                                          gAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAgt                                                                                     <  1:1478170/149‑1 (MQ=255)
                                                            gTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAgtgt                                                                                   <  1:500120/149‑1 (MQ=255)
                                                             ttCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTa                                                                                  <  2:3830295/149‑1 (MQ=255)
                                                             ttCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTa                                                                                  >  2:585426/1‑149 (MQ=255)
                                                               cATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCGTCAGTTAGGAAATAACGCAGACAGTGTAAt                                                                                <  1:3921635/149‑1 (MQ=255)
                                                                aTACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATTCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATc                                                                               <  1:2006972/149‑1 (MQ=255)
                                                                 tACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTATGAAATAACGCAGACAGTGTAATCt                                                                              <  1:1297267/149‑1 (MQ=255)
                                                                 tACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCt                                                                              >  2:1882088/1‑149 (MQ=255)
                                                                  aCGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCtt                                                                             <  2:3613537/149‑1 (MQ=255)
                                                                    ggCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTtta                                                                           <  2:3021481/149‑1 (MQ=255)
                                                                    ggCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTtta                                                                           <  2:964566/149‑1 (MQ=255)
                                                                      cTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTttatt                                                                         <  1:180105/149‑1 (MQ=255)
                                                                       tATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTttatta                                                                        >  2:2573186/1‑149 (MQ=255)
                                                                        aTCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTaa                                                                       >  2:3729299/1‑149 (MQ=255)
                                                                         tCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAAt                                                                      >  2:3121177/1‑149 (MQ=255)
                                                                           gttgttCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCa                                                                    >  2:1212339/1‑149 (MQ=255)
                                                                               ttCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTa                                                                <  2:3851992/149‑1 (MQ=255)
                                                                                 cAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACa                                                              <  2:2847723/149‑1 (MQ=255)
                                                                                   ggACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTAATTAATCAGTTACAAg                                                            >  2:2973452/1‑149 (MQ=255)
                                                                                        ccaccaTGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTa                                                       >  1:2380012/1‑149 (MQ=255)
                                                                                          accaTGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATg                                                     <  2:84775/149‑1 (MQ=255)
                                                                                            caTGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGc                                                   <  2:98615/149‑1 (MQ=255)
                                                                                               gCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAg                                                >  1:1836181/1‑149 (MQ=255)
                                                                                               gCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAg                                                <  2:1304752/149‑1 (MQ=255)
                                                                                                cAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGt                                               <  1:4111342/149‑1 (MQ=255)
                                                                                                    ggcgCACGATGGCGACGTTCCGCCGTATGACTGGGGACATTCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGTAATAACGGAGACAGTGTAATCTTTATTAATCAGTTTCAAGGTTTATGGCCTGTTGAg                                           <  2:1955433/149‑1 (MQ=255)
                                                                                                     gcgcACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGc                                          >  1:387626/1‑149 (MQ=255)
                                                                                                         aCGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTa                                      >  2:2677630/1‑149 (MQ=255)
                                                                                                         aCGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTa                                      >  1:2879504/1‑149 (MQ=255)
                                                                                                              ggCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTAtctct                                 >  2:144235/1‑149 (MQ=255)
                                                                                                                 gACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCac                              >  1:2500165/1‑149 (MQ=255)
                                                                                                                  aCGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCact                             >  2:2766411/1‑149 (MQ=255)
                                                                                                                    gTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCactac                           <  2:2814044/149‑1 (MQ=255)
                                                                                                                     ttCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCactact                          >  2:3634845/1‑149 (MQ=255)
                                                                                                                      tCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTc                         <  2:3318171/149‑1 (MQ=255)
                                                                                                                        cgccgTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCAc                       <  1:3789059/149‑1 (MQ=255)
                                                                                                                        cgccgTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCAc                       <  1:2714936/149‑1 (MQ=255)
                                                                                                                          ccgTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTg                     >  2:1516091/1‑149 (MQ=255)
                                                                                                                          ccgTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTg                     >  2:1517371/1‑149 (MQ=255)
                                                                                                                              aTGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGa                 <  2:1593328/149‑1 (MQ=255)
                                                                                                                              aTGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGa                 >  1:2062632/1‑149 (MQ=255)
                                                                                                                              aTGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGa                 >  1:2184956/1‑149 (MQ=255)
                                                                                                                                gACTGGGGACAATCCCGATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGc               <  1:1395869/149‑1 (MQ=255)
                                                                                                                                  cTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACNGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTg             <  1:2083057/149‑1 (MQ=255)
                                                                                                                                    ggggACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGcc           <  1:3024096/149‑1 (MQ=255)
                                                                                                                                    ggggACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGCGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGcc           >  2:1808608/1‑149 (MQ=255)
                                                                                                                                      ggACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCcgc         >  2:4050074/1‑149 (MQ=255)
                                                                                                                                        aCAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGt       <  2:352155/149‑1 (MQ=255)
                                                                                                                                        aCAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGGTATCTCTCACTACTCACTGGCGAGCTGCCGCGt       >  1:2206015/1‑149 (MQ=255)
                                                                                                                                           aTCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTa    >  1:3271718/1‑149 (MQ=255)
                                                                                                                                           aTCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTa    >  1:3464336/1‑149 (MQ=255)
                                                                                                                                           aTCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTa    >  1:3739791/1‑149 (MQ=255)
                                                                                                                                            tCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTAc   <  2:1306997/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
ACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCGATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTACG  >  CP009273/1481861‑1482150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑