Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,508,681 C→A 100% F198L (TTC→TTA)  ydcU → putative spermidine/putrescine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,508,6810CA98.1% 824.2 / 4.1 259F198L (TTC→TTA) ydcUputative spermidine/putrescine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base A (129/125);  total (130/129)
Fisher's exact test for biased strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 259 total aligned reads displayed.

GATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATC  >  CP009273/1508535‑1508825
                                                                                                                                                  |                                                                                                                                                
gATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAAt                                                                                                                                                <  2:1059541/149‑1 (MQ=255)
gATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAAt                                                                                                                                                <  1:1836427/149‑1 (MQ=255)
   ggCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGAt                                                                                                                                             >  1:4077766/1‑149 (MQ=255)
   ggCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGAt                                                                                                                                             >  1:1367969/1‑149 (MQ=255)
    gCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATc                                                                                                                                            <  1:2337497/149‑1 (MQ=255)
        ggCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCTGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGc                                                                                                                                        >  2:1010267/1‑149 (MQ=255)
        ggCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGc                                                                                                                                        >  2:1009925/1‑149 (MQ=255)
            cAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGt                                                                                                                                    >  1:2391245/1‑149 (MQ=255)
             aGTGGTTTTTACAACATCTTGGGCTGGAACCTCTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGtt                                                                                                                                   <  1:1052811/149‑1 (MQ=255)
             aGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGtt                                                                                                                                   >  1:3257722/1‑149 (MQ=255)
             aGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGtt                                                                                                                                   <  2:3920553/149‑1 (MQ=255)
             aGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGtt                                                                                                                                   <  1:1052642/149‑1 (MQ=255)
              gTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTc                                                                                                                                  <  2:118512/149‑1 (MQ=255)
              gTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTc                                                                                                                                  >  2:3510780/1‑149 (MQ=255)
                 ttttttACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAgg                                                                                                                               >  1:2370305/2‑149 (MQ=255)
                  tttttACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAggc                                                                                                                              >  2:1576737/1‑149 (MQ=255)
                      tACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGgcggcg                                                                                                                          >  1:1557864/1‑149 (MQ=255)
                       acaacaTCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGgcgc                                                                                                                         <  2:2493003/149‑1 (MQ=255)
                       acaacaTCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGgcgc                                                                                                                         >  2:385516/1‑149 (MQ=255)
                        caacaTCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCt                                                                                                                        >  1:1127249/1‑149 (MQ=255)
                        caacaTCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCt                                                                                                                        <  1:3028530/149‑1 (MQ=255)
                         aacaTCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCtt                                                                                                                       >  2:3322567/1‑149 (MQ=255)
                               ttGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGt                                                                                                                 <  1:733742/149‑1 (MQ=255)
                                     tGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTgccg                                                                                                           >  2:4037751/1‑149 (MQ=255)
                                      ggAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTgccgc                                                                                                          >  2:2376684/1‑149 (MQ=255)
                                      ggAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTgccgc                                                                                                          >  1:1692978/1‑149 (MQ=255)
                                       gAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTgccgcc                                                                                                         <  1:3819042/149‑1 (MQ=255)
                                        aaCCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGccgccg                                                                                                        >  1:1682136/1‑149 (MQ=255)
                                         aCCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATTATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGt                                                                                                       >  2:3780782/1‑149 (MQ=255)
                                         aCCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGt                                                                                                       <  2:2175841/149‑1 (MQ=255)
                                             ctgctgACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAtt                                                                                                   >  2:2142668/1‑149 (MQ=255)
                                             ctgctgACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAtt                                                                                                   >  1:3994913/1‑149 (MQ=255)
                                             ctgctgACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAtt                                                                                                   >  2:669846/1‑149 (MQ=255)
                                               gctgACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAttgt                                                                                                 >  1:3346124/1‑149 (MQ=255)
                                               gctgACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAttgt                                                                                                 <  1:217013/149‑1 (MQ=255)
                                               gctgACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAttgt                                                                                                 <  2:2866068/149‑1 (MQ=255)
                                                 tgACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGGTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAttgttg                                                                                               >  1:218079/1‑149 (MQ=255)
                                                   aCTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATTATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCa                                                                                             <  2:592537/149‑1 (MQ=255)
                                                     tGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAgg                                                                                           >  2:2132916/1‑149 (MQ=255)
                                                     tGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAgg                                                                                           >  1:2207272/1‑149 (MQ=255)
                                                      gCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGc                                                                                          >  1:257352/1‑149 (MQ=255)
                                                         ttCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTc                                                                                       <  1:2308405/149‑1 (MQ=255)
                                                            cTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGc                                                                                    <  1:3163599/149‑1 (MQ=255)
                                                              tACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTg                                                                                  <  1:2925537/149‑1 (MQ=255)
                                                               aCATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATATGGTTGCCGTTAATTATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTTCAGGCGTCGGCTGa                                                                                 >  1:280869/1‑149 (MQ=255)
                                                                  ttACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGAtct                                                                              <  2:375571/149‑1 (MQ=255)
                                                                  ttACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGAtct                                                                              <  2:2112839/149‑1 (MQ=255)
                                                                   tACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGAtctc                                                                             <  1:4028351/149‑1 (MQ=255)
                                                                   tACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGAtctc                                                                             >  1:597045/1‑149 (MQ=255)
                                                                    aCCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCg                                                                            >  1:3233195/1‑149 (MQ=255)
                                                                     ccTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCgg                                                                           <  2:1910392/149‑1 (MQ=255)
                                                                      cTGCGGTGGGCGGAAATACGCTGTCAACGTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTAGATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGgc                                                                          <  2:3048102/149‑1 (MQ=255)
                                                                              ggCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTcc                                                                  <  2:1524463/149‑1 (MQ=255)
                                                                               gCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCa                                                                 <  2:3008439/149‑1 (MQ=255)
                                                                               gCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCa                                                                 <  1:1486738/149‑1 (MQ=255)
                                                                               gCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCa                                                                 <  2:3514307/149‑1 (MQ=255)
                                                                                  gAAATACGCTGTCAACTTGCGGGCTGGGGCGCTTTGTGGTGTGTCAGTATATCTGGTTGCCGTTAATGATCCTGCCCCTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATATTTGCAGGCGACGGCTGATCTCGGCGCCCGTCCACGa                                                              >  1:1882259/1‑149 (MQ=255)
                                                                                  gAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTTGTGTTTCTGTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGa                                                              >  1:215176/1‑149 (MQ=255)
                                                                                   aaaTACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGAc                                                             <  1:1199373/149‑1 (MQ=255)
                                                                                      tACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACaaa                                                          >  2:1733361/1‑149 (MQ=255)
                                                                                       aCGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAAc                                                         <  1:385516/149‑1 (MQ=255)
                                                                                        cGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAAcc                                                        <  2:3153885/149‑1 (MQ=255)
                                                                                           tGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCttt                                                     <  1:2639507/149‑1 (MQ=255)
                                                                                              cAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGc                                                  <  2:1265102/149‑1 (MQ=255)
                                                                                              cAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGc                                                  <  2:1442182/149‑1 (MQ=255)
                                                                                                aCTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTa                                                <  2:2580574/149‑1 (MQ=255)
                                                                                                aCTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTa                                                <  2:512873/149‑1 (MQ=255)
                                                                                                 cTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTAt                                               <  2:1841170/149‑1 (MQ=255)
                                                                                                 cTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTAt                                               >  1:3646135/1‑149 (MQ=255)
                                                                                                   tCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATTATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTAtgt                                             <  2:2760923/149‑1 (MQ=255)
                                                                                                   tCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTAtgt                                             <  2:3220242/149‑1 (MQ=255)
                                                                                                     cGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATgtgg                                           <  1:3616599/149‑1 (MQ=255)
                                                                                                      gggCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATgtggt                                          <  2:328990/149‑1 (MQ=255)
                                                                                                        gCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGtgc                                        <  2:1692978/149‑1 (MQ=255)
                                                                                                        gCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGtgc                                        <  2:1692925/149‑1 (MQ=255)
                                                                                                        gCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGtgc                                        <  2:1498323/149‑1 (MQ=255)
                                                                                                           ggggCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGtgctgc                                     >  2:3193733/1‑149 (MQ=255)
                                                                                                            gggCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGcc                                    <  1:3866121/149‑1 (MQ=255)
                                                                                                             ggCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCg                                   >  2:874706/1‑149 (MQ=255)
                                                                                                                 cTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATTATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTgg                               <  1:1975842/149‑1 (MQ=255)
                                                                                                                  tttCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACATACCTTTCGCTATGTGGTGCTGCCGCTGGc                              <  2:2952805/149‑1 (MQ=255)
                                                                                                                       ggTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATcc                         >  1:472202/1‑149 (MQ=255)
                                                                                                                          gtTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCgg                      <  2:1620766/149‑1 (MQ=255)
                                                                                                                           tttCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCggg                     <  1:1102280/149‑1 (MQ=255)
                                                                                                                           tttCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCggg                     >  1:818882/1‑149 (MQ=255)
                                                                                                                            ttCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGt                    >  1:1289515/1‑149 (MQ=255)
                                                                                                                              ctctATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTAt                  <  2:2886798/149‑1 (MQ=255)
                                                                                                                              ctctATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTAt                  <  1:2408449/149‑1 (MQ=255)
                                                                                                                               tctATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTAtt                 <  1:521074/149‑1 (MQ=255)
                                                                                                                                ctATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTg                <  2:390637/149‑1 (MQ=255)
                                                                                                                                    atCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGc            <  1:4046782/149‑1 (MQ=255)
                                                                                                                                    atCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGc            >  2:1511518/1‑149 (MQ=255)
                                                                                                                                    atCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGc            >  2:1511649/1‑149 (MQ=255)
                                                                                                                                     tCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCACTCCCGGGTATTGCCGCt           >  2:2153591/1‑149 (MQ=255)
                                                                                                                                         gTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGct       >  1:2527521/1‑149 (MQ=255)
                                                                                                                                           tGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGctct     <  1:150955/149‑1 (MQ=255)
                                                                                                                                           tGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGctct     <  2:70992/149‑1 (MQ=255)
                                                                                                                                            gCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGctcga    >  1:365509/1‑147 (MQ=255)
                                                                                                                                            gCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTa    >  1:2782046/1‑149 (MQ=255)
                                                                                                                                              cGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATc  <  1:872522/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATC  >  CP009273/1508535‑1508825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑