Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,537,806 A→T 100% L171* (TTA→TAA)  narU ← nitrate/nitrite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,537,8060AT97.8% 878.4 / 6.3 267L171* (TTA→TAA) narUnitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base A (3/3);  new base T (114/147);  total (117/150)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 267 total aligned reads displayed.

GCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATT  >  CP009273/1537660‑1537951
                                                                                                                                                  |                                                                                                                                                 
 cGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATc                                                                                                                                                <  2:3229584/149‑1 (MQ=255)
     aCCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGcc                                                                                                                                            >  1:3464411/1‑149 (MQ=255)
     aCCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGcc                                                                                                                                            <  2:3600171/149‑1 (MQ=255)
        cAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCAtt                                                                                                                                         >  1:3857646/1‑149 (MQ=255)
          aaTCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTaa                                                                                                                                       >  2:754229/1‑149 (MQ=255)
          aaTCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTaa                                                                                                                                       >  2:684566/1‑149 (MQ=255)
           aTCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAAt                                                                                                                                      <  2:2717250/149‑1 (MQ=255)
            tCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATc                                                                                                                                     <  1:154232/149‑1 (MQ=255)
              cATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATccc                                                                                                                                   <  2:861016/149‑1 (MQ=255)
                tGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCaa                                                                                                                                 >  1:897226/1‑149 (MQ=255)
                tGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCaa                                                                                                                                 <  1:1362717/149‑1 (MQ=255)
                tGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCaa                                                                                                                                 >  1:2474907/1‑149 (MQ=255)
                 gCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAag                                                                                                                                <  2:2181375/149‑1 (MQ=255)
                 gCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAag                                                                                                                                <  2:106617/149‑1 (MQ=255)
                 gCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAag                                                                                                                                <  1:4009208/149‑1 (MQ=255)
                 gCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAag                                                                                                                                <  1:1574434/149‑1 (MQ=255)
                   ggCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAagag                                                                                                                              >  2:2771914/1‑149 (MQ=255)
                      aTTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAgcgc                                                                                                                           >  1:1958850/1‑149 (MQ=255)
                          gCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTccc                                                                                                                       <  2:3659641/149‑1 (MQ=255)
                              gCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGt                                                                                                                   <  1:2073782/149‑1 (MQ=255)
                               cGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGtt                                                                                                                  <  2:3332478/149‑1 (MQ=255)
                               cGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGtt                                                                                                                  <  2:2274714/149‑1 (MQ=255)
                                 aCATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTg                                                                                                                >  2:3724305/1‑149 (MQ=255)
                                 aCATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTg                                                                                                                >  2:1349139/1‑149 (MQ=255)
                                    tCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCt                                                                                                             <  2:3531509/149‑1 (MQ=255)
                                         gAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTgg                                                                                                        <  2:2883827/149‑1 (MQ=255)
                                           aCCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGaa                                                                                                      >  2:2969901/1‑149 (MQ=255)
                                                cGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAaagaag                                                                                                 >  1:1939197/1‑149 (MQ=255)
                                                 ggCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAagaaga                                                                                                <  1:2324246/149‑1 (MQ=255)
                                                  gCCTGCGGTACGCCATTGACGCCGAGAAAGTCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTTCATTACACTTACACCTAAGTTTCCTTATACGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAgaataa                                                                                               <  1:354092/149‑1 (MQ=255)
                                                  gCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAgaagaa                                                                                               <  2:1981078/149‑1 (MQ=255)
                                                  gCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAgaagaa                                                                                               >  1:1700542/1‑149 (MQ=255)
                                                   ccTGCGGTACGCCATTTACGCCGATAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGaaa                                                                                              <  1:2333069/149‑1 (MQ=255)
                                                   ccTGCGGTACGCCATTGTCGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGaaa                                                                                              <  2:2035237/149‑1 (MQ=255)
                                                    cTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAAc                                                                                             <  2:2143010/149‑1 (MQ=255)
                                                         gTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGata                                                                                        >  1:325344/1‑149 (MQ=255)
                                                          tACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGatat                                                                                       >  1:2234283/1‑149 (MQ=255)
                                                               cATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGccc                                                                                  <  2:1836898/149‑1 (MQ=255)
                                                                 ttGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCAt                                                                                <  2:1859539/149‑1 (MQ=255)
                                                                   gACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGc                                                                              <  1:3216377/149‑1 (MQ=255)
                                                                   gACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGc                                                                              <  1:178515/149‑1 (MQ=255)
                                                                    aCGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCt                                                                             <  2:3268271/149‑1 (MQ=255)
                                                                     cGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTAAACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTc                                                                            <  2:308669/149‑1 (MQ=255)
                                                                        cGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGaa                                                                         >  1:2030012/1‑149 (MQ=255)
                                                                         gagaAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAg                                                                        <  2:2228458/149‑1 (MQ=255)
                                                                           gaAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCa                                                                      >  1:1741159/1‑149 (MQ=255)
                                                                              aGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAg                                                                   >  2:2245955/1‑149 (MQ=255)
                                                                               ggCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGt                                                                  <  1:3410865/149‑1 (MQ=255)
                                                                                gCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGtt                                                                 <  1:2549916/149‑1 (MQ=255)
                                                                                   aaTACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGc                                                              <  2:1700366/149‑1 (MQ=255)
                                                                                    aTACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCa                                                             >  1:147416/1‑149 (MQ=255)
                                                                                     tACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCAc                                                            <  1:83857/149‑1 (MQ=255)
                                                                                      aCAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCAcc                                                           >  2:1271281/1‑149 (MQ=255)
                                                                                      aCAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCAcc                                                           <  2:477667/149‑1 (MQ=255)
                                                                                         ggTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGc                                                        <  2:445340/149‑1 (MQ=255)
                                                                                         ggTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGc                                                        <  2:2514043/149‑1 (MQ=255)
                                                                                         ggTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGc                                                        <  2:2355099/149‑1 (MQ=255)
                                                                                         ggTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGc                                                        >  2:515232/1‑149 (MQ=255)
                                                                                         ggTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGc                                                        <  2:2999029/149‑1 (MQ=255)
                                                                                             cAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCaaaa                                                    <  1:1281400/149‑1 (MQ=255)
                                                                                                 aaTGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGc                                                <  1:2969901/149‑1 (MQ=255)
                                                                                                    gACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATa                                             >  1:2646364/1‑149 (MQ=255)
                                                                                                      ccAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGc                                           >  2:623636/1‑149 (MQ=255)
                                                                                                        aGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCaa                                         >  2:91449/1‑149 (MQ=255)
                                                                                                        aGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCaa                                         >  1:537253/1‑149 (MQ=255)
                                                                                                        aGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCaa                                         >  1:1255254/1‑149 (MQ=255)
                                                                                                         gCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCaaa                                        <  1:31514/149‑1 (MQ=255)
                                                                                                         gCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCaaa                                        >  2:1546539/1‑149 (MQ=255)
                                                                                                           ggTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGc                                      <  1:2798209/149‑1 (MQ=255)
                                                                                                           ggTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGc                                      >  2:2569496/1‑149 (MQ=255)
                                                                                                               cAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgata                                  >  1:1633748/1‑149 (MQ=255)
                                                                                                                aaCCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgataa                                 >  2:3946287/1‑149 (MQ=255)
                                                                                                                aaCCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgataa                                 <  1:3299946/149‑1 (MQ=255)
                                                                                                                 aCCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgataac                                <  2:3857280/149‑1 (MQ=255)
                                                                                                                 aCCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgataac                                <  1:2403075/149‑1 (MQ=255)
                                                                                                                 aCCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgataac                                <  2:3857729/149‑1 (MQ=255)
                                                                                                                    aGCTGCATTACAGTTACACCTACGTTTCCTTATCCGACATTAATCCCATGAGCGCTCCCGTGTTTGTCTTTTGGAAAGAAGAAACTGATATTGCCCAGGCTCGAAGCAAAGTTTGCAACTGCAAAACCGCATAGCAAAGcgataacgat                             <  2:3671719/149‑1 (MQ=255)
                                                                                                                    aGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgataacgat                             >  2:2679860/1‑149 (MQ=255)
                                                                                                                       tGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATaaa                          >  1:2123046/1‑149 (MQ=255)
                                                                                                                        gCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAGGCGATAACGATAAat                         >  2:1199517/1‑149 (MQ=255)
                                                                                                                          aTTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCACAGCGATAACGATAAatat                       >  2:2426701/1‑149 (MQ=255)
                                                                                                                          aTTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAatat                       <  2:1958850/149‑1 (MQ=255)
                                                                                                                           ttacacttacacCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATc                      <  1:2707031/149‑1 (MQ=255)
                                                                                                                             acacttacacCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATccc                    <  1:1007370/149‑1 (MQ=255)
                                                                                                                              cacttacacCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCa                   >  1:2574607/1‑149 (MQ=255)
                                                                                                                               acttacacCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCaa                  >  2:1797467/1‑149 (MQ=255)
                                                                                                                                cttacacCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCaaa                 >  2:554369/1‑149 (MQ=255)
                                                                                                                                  tacacCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAg               <  1:766682/149‑1 (MQ=255)
                                                                                                                                    cacCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGa             >  1:1135704/1‑149 (MQ=255)
                                                                                                                                       cTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTa          >  1:23037/1‑149 (MQ=255)
                                                                                                                                       cTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTa          <  2:780735/149‑1 (MQ=255)
                                                                                                                                       cTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTa          >  1:784022/1‑149 (MQ=255)
                                                                                                                                        tAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTAt         >  2:312016/1‑149 (MQ=255)
                                                                                                                                         aaGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTAtt        >  2:3766079/1‑149 (MQ=255)
                                                                                                                                         aaGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTAtt        <  1:2770717/149‑1 (MQ=255)
                                                                                                                                         aaGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTAtt        <  1:2771914/149‑1 (MQ=255)
                                                                                                                                             ttCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGa    >  1:671254/1‑149 (MQ=255)
                                                                                                                                             ttCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTGGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGa    >  1:3833634/1‑149 (MQ=255)
                                                                                                                                              tCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGAt   >  2:3186183/1‑149 (MQ=255)
                                                                                                                                               ccTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGAtt  <  1:359825/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATT  >  CP009273/1537660‑1537951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑