BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,548,144	G→T	100%	intergenic (‑49/+85)	adhP ← / ← maeA	ethanol‑active dehydrogenase/acetaldehyde‑active reductase/malate dehydrogenase, (decarboxylating, NAD‑requiring) (malic enzyme)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,548,144	0	G	T	99.6%	722.1 / ‑4.3	220	intergenic (‑49/+85)	adhP/maeA	ethanol‑active dehydrogenase/acetaldehyde‑active reductase/malate dehydrogenase, (decarboxylating, NAD‑requiring) (malic enzyme)
Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (120/99); total (121/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 224 total aligned reads displayed.

CACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCC  >  CP009273/1547998‑1548289

cacTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGatat                                                                                                                                                 <  1:1185251/149‑1 (MQ=255)

 acTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATAtt                                                                                                                                                <  1:2255626/149‑1 (MQ=255)

 acTCCATTGTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATAtt                                                                                                                                                <  2:2114669/149‑1 (MQ=255)

  cTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATAttt                                                                                                                                               <  1:2556826/149‑1 (MQ=255)

  cTCCATGTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATAttt                                                                                                                                               <  1:3627124/149‑1 (MQ=255)

    ccATTGTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTAt                                                                                                                                             <  1:2474928/149‑1 (MQ=255)

     cATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAtatttatt                                                                                                                                            <  2:2585638/149‑1 (MQ=255)

     cATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAtatttatt                                                                                                                                            <  2:1349815/149‑1 (MQ=255)

      aTTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTc                                                                                                                                           <  1:1112183/149‑1 (MQ=255)

           cagcagGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCAc                                                                                                                                      <  2:1478773/149‑1 (MQ=255)

              cagGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTAc                                                                                                                                   >  1:3763151/1‑149 (MQ=255)

                gggCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTAccc                                                                                                                                 >  1:2409498/1‑149 (MQ=255)

                  gCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAg                                                                                                                               >  1:3188997/1‑149 (MQ=255)

                    ttCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTa                                                                                                                             <  2:3681140/149‑1 (MQ=255)

                       gCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATgg                                                                                                                          <  2:914615/149‑1 (MQ=255)

                        ccATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGa                                                                                                                         >  2:702375/1‑149 (MQ=255)

                        ccATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGa                                                                                                                         <  2:1546108/149‑1 (MQ=255)

                        ccATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGa                                                                                                                         <  2:1545778/149‑1 (MQ=255)

                        ccATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGa                                                                                                                         >  2:1414726/1‑149 (MQ=255)

                             tttCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATgaga                                                                                                                    >  1:3869408/1‑149 (MQ=255)

                              ttCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGAt                                                                                                                   <  2:1704662/149‑1 (MQ=255)

                                  gTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGa                                                                                                               >  2:3720685/1‑149 (MQ=255)

                                  gTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGa                                                                                                               >  1:1804302/1‑149 (MQ=255)

                                  gTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGa                                                                                                               <  2:3867350/149‑1 (MQ=255)

                                  gTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGa                                                                                                               <  2:2927656/149‑1 (MQ=255)

                                      gcgcAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGaaaaa                                                                                                           >  1:3793258/1‑149 (MQ=255)

                                        gcAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAgg                                                                                                         >  1:4063426/1‑149 (MQ=255)

                                         cAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAggg                                                                                                        <  2:1884240/149‑1 (MQ=255)

                                               tttATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAggg                                                                                                  <  2:2727536/149‑1 (MQ=255)

                                               tttATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAggg                                                                                                  <  2:2158396/149‑1 (MQ=255)

                                                ttATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGa                                                                                                 <  1:925405/149‑1 (MQ=255)

                                                    aCGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATa                                                                                             >  2:1500372/1‑149 (MQ=255)

                                                    aCGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATa                                                                                             <  2:2612233/149‑1 (MQ=255)

                                                      gTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGc                                                                                           >  1:3116823/1‑149 (MQ=255)

                                                      gTAACGTCAAAATGATGATCCTTCTTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCTGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGc                                                                                           <  2:3397666/149‑1 (MQ=255)

                                                       tAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGcc                                                                                          >  2:2539524/1‑149 (MQ=255)

                                                        aaCGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGccc                                                                                         >  2:2310749/1‑149 (MQ=255)

                                                        aaCGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGccc                                                                                         <  2:2999978/149‑1 (MQ=255)

                                                               aCATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGcc                                                                                  <  1:3699237/149‑1 (MQ=255)

                                                               aCATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGcc                                                                                  <  2:2845816/149‑1 (MQ=255)

                                                               aCATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGcc                                                                                  <  1:3699529/149‑1 (MQ=255)

                                                                cATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCt                                                                                 >  1:3300474/1‑149 (MQ=255)

                                                                  tgatgaTCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTc                                                                               >  2:3394461/1‑149 (MQ=255)

                                                                  tgatgaTCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTc                                                                               >  2:1380116/1‑149 (MQ=255)

                                                                   gatgatCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCa                                                                              <  1:1797714/149‑1 (MQ=255)

                                                                    atgatCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCAc                                                                             >  2:2565102/1‑149 (MQ=255)

                                                                       atCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACttc                                                                          <  2:2446341/149‑3 (MQ=255)

                                                                        tCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTcc                                                                         >  2:445279/1‑149 (MQ=255)

                                                                         ccTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGTAAATAGCCCGGTAGCCTTCACTTCCg                                                                        >  1:1853075/1‑149 (MQ=255)

                                                                         ccTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCg                                                                        <  2:2126842/149‑1 (MQ=255)

                                                                            tCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGgc                                                                     >  2:4057906/1‑149 (MQ=255)

                                                                                 acaacTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAgg                                                                <  2:1939525/149‑1 (MQ=255)

                                                                                   aacTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCt                                                              >  1:2807975/1‑149 (MQ=255)

                                                                                   aacTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCt                                                              >  2:1596548/1‑149 (MQ=255)

                                                                                    acTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCtt                                                             <  2:1984292/149‑1 (MQ=255)

                                                                                    acTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCtt                                                             <  1:4032601/149‑1 (MQ=255)

                                                                                       gcagcaTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGa                                                          <  1:1074103/149‑1 (MQ=255)

                                                                                         aGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATg                                                        <  1:1981980/149‑1 (MQ=255)

                                                                                             ttCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAgg                                                    >  2:1697657/1‑149 (MQ=255)

                                                                                              tCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGt                                                   >  2:1797248/1‑149 (MQ=255)

                                                                                                 tAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTAcg                                                >  1:725788/1‑149 (MQ=255)

                                                                                                 tAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTAcg                                                >  1:1185766/1‑149 (MQ=255)

                                                                                                 tAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTAc                                                 <  1:1333063/148‑1 (MQ=255)

                                                                                                  aGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTAcgg                                               >  1:2125075/1‑149 (MQ=255)

                                                                                                  aGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTAcgg                                               >  1:1413715/1‑149 (MQ=255)

                                                                                                   gTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTAcggc                                              >  2:1437549/1‑149 (MQ=255)

                                                                                                   gTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTAcggc                                              >  2:1285077/1‑149 (MQ=255)

                                                                                                     tcctccTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTAcggcgg                                            <  1:2670672/149‑1 (MQ=255)

                                                                                                     tcctccTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTAcggcgg                                            >  2:2259512/1‑149 (MQ=255)

                                                                                                       ctcctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTa                                          >  1:1297515/1‑149 (MQ=255)

                                                                                                         cctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGt                                        >  2:3460868/1‑149 (MQ=255)

                                                                                                          ctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTc                                       <  2:592053/149‑1 (MQ=255)

                                                                                                          ctTATCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTc                                       <  2:2169780/149‑1 (MQ=255)

                                                                                                           ttttCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTcg                                      >  2:3963660/1‑149 (MQ=255)

                                                                                                           ttttCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTcg                                      >  1:1409147/1‑149 (MQ=255)

                                                                                                            tttCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTcgc                                     >  2:482007/1‑149 (MQ=255)

                                                                                                                ggATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTa                                 <  1:735543/149‑1 (MQ=255)

                                                                                                                 gatgatGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGTAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTAt                                <  2:1853075/149‑1 (MQ=255)

                                                                                                                 gatgatGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTAt                                >  2:588077/1‑149 (MQ=255)

                                                                                                                 gatgatGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTAt                                <  1:585920/149‑1 (MQ=255)

                                                                                                                 gatgatGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTAt                                >  2:1082183/1‑149 (MQ=255)

                                                                                                                   tgatgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTc                              >  1:2484731/1‑149 (MQ=255)

                                                                                                                    gatgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCg                             >  1:2128453/1‑149 (MQ=255)

                                                                                                                       gTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCt                          <  1:2062003/149‑1 (MQ=255)

                                                                                                                        ttCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCtt                         <  1:2003720/149‑1 (MQ=255)

                                                                                                                          cTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGc                       <  1:803865/149‑1 (MQ=255)

                                                                                                                          cTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGc                       <  2:3927247/149‑1 (MQ=255)

                                                                                                                          cTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGc                       <  2:128438/149‑1 (MQ=255)

                                                                                                                               tAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGaa                  >  2:1744857/1‑149 (MQ=255)

                                                                                                                                aGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGaaa                 <  1:928536/149‑1 (MQ=255)

                                                                                                                                aGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGaaa                 <  1:1746402/149‑1 (MQ=255)

                                                                                                                                 gCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAAt                >  1:2188873/1‑149 (MQ=255)

                                                                                                                                   aGGTGAGTAATATGATATTTATTATCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATTGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTa              <  2:1318364/149‑1 (MQ=255)

                                                                                                                                    ggTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAt             >  2:1703257/1‑149 (MQ=255)

                                                                                                                                           cAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAATTAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAAt      <  2:3231980/149‑1 (MQ=255)

                                                                                                                                           cAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAAt      <  1:1500372/149‑1 (MQ=255)

                                                                                                                                            aaaTGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATg     >  1:2945108/1‑149 (MQ=255)

                                                                                                                                              aTGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGc   >  2:3636118/1‑149 (MQ=255)

                                                                                                                                               tGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGcc  <  1:453244/149‑1 (MQ=255)

                                                                                                                                               tGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTTCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGcc  <  2:226346/149‑1 (MQ=255)

CACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCC  >  CP009273/1547998‑1548289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`