BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,580,852	C→A	100%	intergenic (‑109/+225)	ydeP ← / ← ydeQ	putative oxidoreductase/putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,580,852	0	C	A	98.7%	765.6 / 0.3	233	intergenic (‑109/+225)	ydeP/ydeQ	putative oxidoreductase/putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand): ref base C (0/3); new base A (99/131); total (99/134)
Fisher's exact test for biased strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 235 total aligned reads displayed.

CCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTCTCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGATTTTCTTACAG  >  CP009273/1580706‑1580995

ccTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTActatc                                                                                                                                               <  2:1181554/149‑1 (MQ=255)

    cagcaCCCTTGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTc                                                                                                                                           <  2:1256933/149‑1 (MQ=255)

    cagcaCCCGGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTc                                                                                                                                           <  2:2843272/149‑1 (MQ=255)

     agcaCCGTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCt                                                                                                                                          <  2:2712838/149‑1 (MQ=255)

     agcaCCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCt                                                                                                                                          <  1:3689604/149‑1 (MQ=255)

      gcaCCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTa                                                                                                                                         >  1:599533/1‑149 (MQ=255)

          ccTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTAtttt                                                                                                                                     >  1:2765248/1‑149 (MQ=255)

          ccTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCGGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTAtttt                                                                                                                                     <  1:135857/149‑1 (MQ=255)

             tgTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATTAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTaa                                                                                                                                  <  2:4088479/148‑1 (MQ=255)

             ggTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTaa                                                                                                                                  >  1:2370452/1‑149 (MQ=255)

               tAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATc                                                                                                                                <  1:2031521/149‑1 (MQ=255)

                  gATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCtat                                                                                                                             >  1:902950/1‑149 (MQ=255)

                  gATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCtat                                                                                                                             >  1:3699993/1‑149 (MQ=255)

                   aTTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCtatt                                                                                                                            <  1:2735557/149‑1 (MQ=255)

                       aaTTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCg                                                                                                                        >  2:1334062/1‑149 (MQ=255)

                       aaTTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCg                                                                                                                        >  2:2936418/1‑149 (MQ=255)

                        aTTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGt                                                                                                                       <  2:1231885/149‑1 (MQ=255)

                        aTTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGt                                                                                                                       <  1:3538280/149‑1 (MQ=255)

                            ttttCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTaaa                                                                                                                   <  1:2279246/149‑1 (MQ=255)

                              ttcttcATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACa                                                                                                                 >  1:1630631/1‑149 (MQ=255)

                              ttcttcATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACa                                                                                                                 >  2:1801985/1‑149 (MQ=255)

                                   cATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGa                                                                                                            <  1:2733583/149‑1 (MQ=255)

                                     tCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGTATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGAtt                                                                                                          <  2:1471564/149‑1 (MQ=255)

                                     tCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGAtt                                                                                                          <  2:3829807/149‑1 (MQ=255)

                                      cTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTa                                                                                                         >  2:632267/1‑149 (MQ=255)

                                       tACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTaa                                                                                                        >  1:1136081/1‑149 (MQ=255)

                                       tACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTaa                                                                                                        >  2:1741754/1‑149 (MQ=255)

                                         cTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAAt                                                                                                      <  2:1029559/149‑1 (MQ=255)

                                         cTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAAt                                                                                                      >  1:3758696/1‑149 (MQ=255)

                                          ttATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATa                                                                                                     <  2:599533/149‑1 (MQ=255)

                                           tATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATAc                                                                                                    >  1:3445652/1‑149 (MQ=255)

                                             tCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGa                                                                                                  >  2:2413552/1‑149 (MQ=255)

                                             tCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGa                                                                                                  <  2:1579307/149‑1 (MQ=255)

                                             tCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGa                                                                                                  <  1:1004299/149‑1 (MQ=255)

                                             tCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGa                                                                                                  <  2:3625644/149‑1 (MQ=255)

                                             tCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGa                                                                                                  >  1:713549/1‑149 (MQ=255)

                                               cTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATa                                                                                                <  1:1465540/149‑1 (MQ=255)

                                               cTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATa                                                                                                <  1:2247898/149‑1 (MQ=255)

                                                        aaTGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGca                                                                                       <  1:3529433/149‑1 (MQ=255)

                                                          tGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGcatc                                                                                     <  1:62102/149‑1 (MQ=255)

                                                           gAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGcatca                                                                                    <  1:489707/149‑1 (MQ=255)

                                                           gAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGcatca                                                                                    <  1:3476444/149‑1 (MQ=255)

                                                           gAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGcatca                                                                                    >  2:1820864/1‑149 (MQ=255)

                                                             attattAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCatcatc                                                                                  >  2:2180422/1‑149 (MQ=255)

                                                              ttattaGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCAtct                                                                                 >  1:210803/1‑149 (MQ=255)

                                                              ttattaGGATTTGTAATAGCGATGATGCACAGTACATAAAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATATTTCTATTTTTATTCTATTATAGTTAAACAATAGATTAAATACGAAAAGTTAAGCATCAtct                                                                                 >  2:3847042/1‑149 (MQ=255)

                                                                 ttaGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCAtctctc                                                                              >  2:340848/1‑149 (MQ=255)

                                                                  taGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCa                                                                             >  1:2191151/1‑149 (MQ=255)

                                                                     gATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAg                                                                          <  2:1954048/149‑1 (MQ=255)

                                                                     gATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAg                                                                          >  1:1491271/1‑149 (MQ=255)

                                                                      aTTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAgt                                                                         <  1:2493939/149‑1 (MQ=255)

                                                                      aTTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAgt                                                                         <  2:3733388/149‑1 (MQ=255)

                                                                      aTTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAgt                                                                         <  1:1820864/149‑1 (MQ=255)

                                                                       tttGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAgtg                                                                        >  1:3295113/1‑149 (MQ=255)

                                                                       tttGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAgtg                                                                        >  1:95816/1‑149 (MQ=255)

                                                                           tAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTa                                                                    >  1:3479148/1‑149 (MQ=255)

                                                                             aTAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTaaa                                                                  >  2:3496157/1‑149 (MQ=255)

                                                                             aTAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTaaa                                                                  >  1:2629910/1‑149 (MQ=255)

                                                                               agagatgatGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAAtt                                                                <  2:3221563/146‑1 (MQ=255)

                                                                                 cGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTaa                                                              <  1:2305155/149‑1 (MQ=255)

                                                                                  gatgatGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAAc                                                             <  2:1481290/149‑1 (MQ=255)

                                                                                   atgatgCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACa                                                            >  1:3005767/1‑149 (MQ=255)

                                                                                    tgatgCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACat                                                           <  1:112656/149‑1 (MQ=255)

                                                                                    tgatgCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACat                                                           <  1:2542143/149‑1 (MQ=255)

                                                                                    tgatgCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACat                                                           <  1:2834617/149‑1 (MQ=255)

                                                                                     gatgCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACata                                                          <  1:3983870/149‑1 (MQ=255)

                                                                                        gCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATAttt                                                       >  2:2190145/1‑149 (MQ=255)

                                                                                         caaaGTACATACTAATGGATTATCGCATACATGGATATATAATTGTATGTGACTACTATCTTTCTATTTTTAATCTATTATCTTTAAACAATAGATTAAATACGATAAGTTAAGCATCATATCTCATAGTGTTTAATTTAAAATAtttt                                                      <  2:154690/146‑1 (MQ=255)

                                                                                          acaGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATAttttt                                                     >  2:342900/1‑149 (MQ=255)

                                                                                           caGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTc                                                    >  1:642335/1‑149 (MQ=255)

                                                                                            aGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCa                                                   <  1:1737604/149‑1 (MQ=255)

                                                                                             gTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTCTCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCaa                                                  <  2:547175/149‑1 (MQ=255)

                                                                                             gTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCaa                                                  <  1:1459063/149‑1 (MQ=255)

                                                                                                    caAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATaaaa                                           <  2:1441520/149‑1 (MQ=255)

                                                                                                     aaaGGGATTATCGCCCACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATaaaaa                                          >  2:48621/1‑149 (MQ=255)

                                                                                                        gggATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATa                                       <  1:543988/149‑1 (MQ=255)

                                                                                                          gATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAg                                     >  1:2125783/1‑149 (MQ=255)

                                                                                                           aTTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGa                                    <  2:2466862/149‑1 (MQ=255)

                                                                                                              aTCGCCAACATGTATATATAAGAGTAGGGGACTACTATCTTTATATTTTTAATCTATTATCGTTAAACAATAGATTAAATACTATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGActc                                 <  1:1169748/149‑1 (MQ=255)

                                                                                                              aTCGCCAACATGGATATATAAGAGTAGGGGAATACTATCTTTATATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGActc                                 <  1:3030834/149‑1 (MQ=255)

                                                                                                               tCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCTTAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGActct                                >  1:2374700/1‑149 (MQ=255)

                                                                                                               tCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGActct                                >  2:950504/1‑149 (MQ=255)

                                                                                                                cGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTg                               <  1:3931230/149‑1 (MQ=255)

                                                                                                                    aaCATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCtt                           <  1:439705/149‑1 (MQ=255)

                                                                                                                     aCATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTc                          <  1:3401979/149‑1 (MQ=255)

                                                                                                                      cATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCa                         >  2:3087173/1‑149 (MQ=255)

                                                                                                                        tGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAAt                       <  1:604666/149‑1 (MQ=255)

                                                                                                                        tGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAAACTCTGGCttaatt                       >  1:2725447/1‑145 (MQ=255)

                                                                                                                          gATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATtg                     >  2:830223/1‑149 (MQ=255)

                                                                                                                             atataAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTgc                  >  2:1672268/1‑149 (MQ=255)

                                                                                                                             atataAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTgc                  >  1:1956274/1‑149 (MQ=255)

                                                                                                                              tataAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTgcg                 >  1:93899/1‑149 (MQ=255)

                                                                                                                               ataAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTgcgc                <  2:967440/149‑1 (MQ=255)

                                                                                                                                  agagTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGa             >  1:2014040/1‑149 (MQ=255)

                                                                                                                                   gagTAGGGGACTACTATCTTTCTATTTTTAATATATTATCTTTAAAAAATAGATTAAATACGATAAGTTAACCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGAt            <  1:1801985/149‑1 (MQ=255)

                                                                                                                                       aGGGGACTACTATATTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGATTTTc        <  1:78211/149‑1 (MQ=255)

                                                                                                                                        ggggACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGATTTTCt       <  2:3006576/149‑1 (MQ=255)

                                                                                                                                         gggACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGATTTTCtt      <  2:2490953/149‑1 (MQ=255)

                                                                                                                                         gggACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGATTTTCtt      >  1:3634098/1‑149 (MQ=255)

                                                                                                                                             ctactaTCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGATTTTCTTACAg  >  2:3586281/1‑149 (MQ=255)

CCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTCTCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGATTTTCTTACAG  >  CP009273/1580706‑1580995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`