Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,614,089 G→T 100% E87* (GAG→TAG)  marA → multiple antibiotic resistance transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,614,0890GT98.5% 849.9 / 3.0 262E87* (GAG→TAG) marAmultiple antibiotic resistance transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (146/112);  total (148/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 262 total aligned reads displayed.

CCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCA  >  CP009273/1613943‑1614234
                                                                                                                                                  |                                                                                                                                                 
 cAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGt                                                                                                                                                <  2:1016705/149‑1 (MQ=255)
   aaTGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCg                                                                                                                                              >  1:2836120/1‑149 (MQ=255)
      ggCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGcaa                                                                                                                                           >  1:179625/1‑149 (MQ=255)
      ggCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGcaa                                                                                                                                           <  1:540816/149‑1 (MQ=255)
         aCCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGcaacaa                                                                                                                                        >  2:780222/1‑149 (MQ=255)
         aCCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGcaacaa                                                                                                                                        >  2:1987437/1‑149 (MQ=255)
          ccTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACaaa                                                                                                                                       >  2:3542528/1‑149 (MQ=255)
           cTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAAc                                                                                                                                      <  1:2038539/149‑1 (MQ=255)
                aCGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGa                                                                                                                                 >  2:2519825/1‑149 (MQ=255)
                    aTGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCg                                                                                                                             >  2:4074124/1‑149 (MQ=255)
                     tGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGa                                                                                                                            >  1:3665764/1‑149 (MQ=255)
                       tttAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAACTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAAc                                                                                                                          <  1:2118164/149‑1 (MQ=255)
                        ttAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAAcc                                                                                                                         <  1:2092649/149‑1 (MQ=255)
                           aaaaaGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTc                                                                                                                      >  1:2286529/1‑149 (MQ=255)
                              aaGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCaaa                                                                                                                   >  1:1267487/1‑149 (MQ=255)
                                   aCCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTa                                                                                                              <  2:4024876/149‑1 (MQ=255)
                                   aCCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTa                                                                                                              <  2:3589742/149‑1 (MQ=255)
                                   aCCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTa                                                                                                              <  1:3473691/149‑1 (MQ=255)
                                       gTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACttt                                                                                                          <  2:318508/149‑1 (MQ=255)
                                       gTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACttt                                                                                                          >  2:523040/1‑149 (MQ=255)
                                         cattcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAACGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGa                                                                                                        <  2:3959063/149‑1 (MQ=255)
                                         cattcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGa                                                                                                        <  2:1298684/149‑1 (MQ=255)
                                         cattcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGa                                                                                                        <  2:3351126/149‑1 (MQ=255)
                                          attcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGAt                                                                                                       <  1:3796555/149‑1 (MQ=255)
                                          attcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGAt                                                                                                       <  1:2930499/149‑1 (MQ=255)
                                           ttcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAACAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATg                                                                                                      <  2:2656313/149‑1 (MQ=255)
                                           ttcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATg                                                                                                      >  1:2028039/1‑149 (MQ=255)
                                             cattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGtt                                                                                                    <  2:20991/149‑1 (MQ=255)
                                              attAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTc                                                                                                   >  1:683016/1‑149 (MQ=255)
                                              attAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTc                                                                                                   <  1:3217989/149‑1 (MQ=255)
                                                  ggCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTccgcc                                                                                               >  1:4110465/1‑149 (MQ=255)
                                                  ggCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTccgcc                                                                                               <  1:4022667/149‑1 (MQ=255)
                                                  ggCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTccgcc                                                                                               >  1:1089492/1‑149 (MQ=255)
                                                  ggCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTccgcc                                                                                               <  1:3015575/149‑1 (MQ=255)
                                                     cAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCa                                                                                            >  1:729811/1‑149 (MQ=255)
                                                       aTACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATa                                                                                          >  2:3800866/1‑149 (MQ=255)
                                                        tACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATaa                                                                                         >  2:70872/1‑149 (MQ=255)
                                                        tACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATaa                                                                                         >  1:2409829/1‑149 (MQ=255)
                                                         aCATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATaaa                                                                                        >  1:292464/1‑149 (MQ=255)
                                                         aCATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATaaa                                                                                        >  2:643112/1‑149 (MQ=255)
                                                         aCATCCGCAGCCGTAAGATGACAGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATaaa                                                                                        >  1:698874/1‑149 (MQ=255)
                                                          cATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGACCGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAAt                                                                                       <  2:1376491/149‑1 (MQ=255)
                                                                   ccGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGa                                                                              <  2:3289122/149‑1 (MQ=255)
                                                                    cGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGGAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGAc                                                                             <  2:2981165/149‑1 (MQ=255)
                                                                     gTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGAcc                                                                            >  2:1173875/1‑149 (MQ=255)
                                                                     gTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGAcc                                                                            >  2:1400915/1‑149 (MQ=255)
                                                                      tAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCa                                                                           >  2:1879624/1‑149 (MQ=255)
                                                                       aaGGTGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTAGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCaa                                                                          <  2:1301899/149‑1 (MQ=255)
                                                                       aaGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACGTTGATGTTCCGCCGCATAAATACCGGATGACCaa                                                                          >  1:2543194/1‑149 (MQ=255)
                                                                        aGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAat                                                                         >  2:2795635/1‑149 (MQ=255)
                                                                           tGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCACATAAATACCGGATGACCAATATg                                                                      >  2:3080967/1‑149 (MQ=255)
                                                                             aCGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCa                                                                    >  2:1514105/1‑149 (MQ=255)
                                                                                 aaaTCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCGTAAATACCGGATGACCAATATGCAGGGc                                                                >  1:1035403/1‑149 (MQ=255)
                                                                                     cgcgCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAAt                                                            <  1:4079094/149‑1 (MQ=255)
                                                                                     cgcgCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAAt                                                            <  1:2042614/149‑1 (MQ=255)
                                                                                      gcgcAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATc                                                           <  1:2536851/149‑1 (MQ=255)
                                                                                           aaaGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCt                                                      >  1:869743/1‑149 (MQ=255)
                                                                                           aaaGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCt                                                      <  2:820375/149‑1 (MQ=255)
                                                                                              gCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCtttt                                                   <  1:2351925/149‑1 (MQ=255)
                                                                                              gCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCtttt                                                   >  1:2399084/1‑149 (MQ=255)
                                                                                               cTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCttttt                                                  <  2:727304/149‑1 (MQ=255)
                                                                                                tGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTa                                                 <  2:3040468/149‑1 (MQ=255)
                                                                                                tGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTa                                                 >  2:2284108/1‑149 (MQ=255)
                                                                                                tGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTGTTTa                                                 >  2:1471873/1‑149 (MQ=255)
                                                                                                   aGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACAt                                              <  2:593319/149‑1 (MQ=255)
                                                                                                       aaGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCAt                                          <  1:2591860/149‑1 (MQ=255)
                                                                                                       aaGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCAt                                          <  1:1728869/149‑1 (MQ=255)
                                                                                                        aGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCAtt                                         >  2:1263550/1‑149 (MQ=255)
                                                                                                           aaCGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTaaa                                      <  2:3143925/149‑1 (MQ=255)
                                                                                                           aaCGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTaaa                                      <  2:3208246/149‑1 (MQ=255)
                                                                                                            aCGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAAt                                     >  1:3753071/1‑149 (MQ=255)
                                                                                                               aGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCAt                                  <  1:4074124/149‑1 (MQ=255)
                                                                                                                gCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCAtt                                 >  2:26992/1‑149 (MQ=255)
                                                                                                                 ccGATACTCTATCTGGCAGAACGATATGGCTTATAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCAGCCGCATAAATACCGGATGACCATTATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTa                                <  2:1570178/149‑1 (MQ=255)
                                                                                                                  cGATACTCTATCTGGCAGCACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTac                               <  2:1828249/149‑1 (MQ=255)
                                                                                                                  cGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTac                               >  1:1534545/1‑149 (MQ=255)
                                                                                                                   gATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCCTTACATCATTaca                              >  2:2133824/1‑149 (MQ=255)
                                                                                                                   gATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTaca                              >  2:4022210/1‑149 (MQ=255)
                                                                                                                    aTACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTacaa                             >  2:3885168/1‑149 (MQ=255)
                                                                                                                     tACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTacaac                            <  2:1082444/149‑1 (MQ=255)
                                                                                                                     tACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTacaac                            >  2:1639478/1‑149 (MQ=255)
                                                                                                                       ctctATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAg                          >  2:1431189/1‑149 (MQ=255)
                                                                                                                       ctctATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAg                          >  1:843210/1‑149 (MQ=255)
                                                                                                                           aTCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAg                      <  2:1267487/149‑1 (MQ=255)
                                                                                                                           aTCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAg                      <  1:2412266/149‑1 (MQ=255)
                                                                                                                            tCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGt                     >  2:3510327/1‑149 (MQ=255)
                                                                                                                             cTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGtt                    >  1:3166420/1‑149 (MQ=255)
                                                                                                                                  aGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGaaaa               >  1:199200/1‑149 (MQ=255)
                                                                                                                                  aGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGaaaa               >  1:199611/1‑149 (MQ=255)
                                                                                                                                  aGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGaaaa               <  1:2710878/149‑1 (MQ=255)
                                                                                                                                   gAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAAc              >  2:860566/1‑149 (MQ=255)
                                                                                                                                   gAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAAc              >  1:1518130/1‑149 (MQ=255)
                                                                                                                                       gATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGa          >  2:1934345/1‑149 (MQ=255)
                                                                                                                                          atGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACaa       >  1:468836/1‑149 (MQ=255)
                                                                                                                                           tGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAAc      >  1:1390050/1‑149 (MQ=255)
                                                                                                                                            ggCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACg     >  1:1929170/1‑149 (MQ=255)
                                                                                                                                             gCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGt    <  1:3866838/149‑1 (MQ=255)
                                                                                                                                             gCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGt    <  1:1215740/149‑1 (MQ=255)
                                                                                                                                              cTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTc   <  1:224050/149‑1 (MQ=255)
                                                                                                                                               ttCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCa  >  2:2906679/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCA  >  CP009273/1613943‑1614234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑