Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,699,862 T→A 100% L119* (TTG→TAG)  ydgK → DUF2569 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,699,8620TA99.2% 768.1 / ‑2.7 233L119* (TTG→TAG) ydgKDUF2569 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base A (121/110);  total (121/112)
Fisher's exact test for biased strand distribution p-value = 2.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 234 total aligned reads displayed.

TATTACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTT  >  CP009273/1699714‑1700007
                                                                                                                                                    |                                                                                                                                                 
tATTACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGtt                                                                                                                                                   <  1:2116107/149‑1 (MQ=255)
     cacacTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGt                                                                                                                                              <  2:46624/149‑1 (MQ=255)
      acacTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGtt                                                                                                                                             >  1:3625225/1‑149 (MQ=255)
        acTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTa                                                                                                                                           <  2:1487826/149‑1 (MQ=255)
          tgtgGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACt                                                                                                                                         >  2:761591/1‑149 (MQ=255)
          tgtgGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACt                                                                                                                                         <  1:1506600/149‑1 (MQ=255)
          tgtgGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACt                                                                                                                                         <  2:3781570/149‑1 (MQ=255)
              gTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTttgt                                                                                                                                     >  2:3795145/1‑149 (MQ=255)
              gTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTttgt                                                                                                                                     <  1:1553998/149‑1 (MQ=255)
              gTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTttgt                                                                                                                                     >  2:2923575/1‑149 (MQ=255)
              gTTGACCATCGCTTTCTTTAAACGTCGGCCTTGTGTTCCTAAACACTATATTATCTTGCTGCTTATTTCAGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGTAATATGGCATAGCCGATCGCCAGTAGCTGTTTAAAttgt                                                                                                                                     >  2:1753300/1‑149 (MQ=255)
               ttGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTttgtt                                                                                                                                    <  2:1208842/149‑1 (MQ=255)
               ttGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTttgtt                                                                                                                                    <  2:2404653/149‑1 (MQ=255)
                tGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTttgttg                                                                                                                                   >  2:3744578/1‑149 (MQ=255)
                 gACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTgg                                                                                                                                  <  2:3940156/149‑1 (MQ=255)
                  aCCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGc                                                                                                                                 >  1:29407/1‑149 (MQ=255)
                  aCCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGc                                                                                                                                 >  2:219262/1‑149 (MQ=255)
                     aTCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAAc                                                                                                                              >  2:3599809/1‑149 (MQ=255)
                      tCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAAcc                                                                                                                             >  2:3848965/1‑149 (MQ=255)
                         ctttctttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCa                                                                                                                          <  2:1194755/149‑1 (MQ=255)
                         ctttctttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCa                                                                                                                          >  1:2845344/1‑149 (MQ=255)
                                 aaaCGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAATCATTCGCTTTTTCAACCGTGGAAGATTGCATAGACGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATAGt                                                                                                                  >  1:931963/1‑149 (MQ=255)
                                 aaaCGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGt                                                                                                                  >  1:178569/1‑149 (MQ=255)
                                 aaaCGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGt                                                                                                                  >  1:3750665/1‑149 (MQ=255)
                                   aCGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTcc                                                                                                                >  2:2369829/1‑149 (MQ=255)
                                   aCGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTcc                                                                                                                <  1:638808/149‑1 (MQ=255)
                                    cGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTccc                                                                                                               >  2:3987504/1‑149 (MQ=255)
                                         gCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTAtt                                                                                                          <  2:1419248/149‑1 (MQ=255)
                                         gCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTAtt                                                                                                          <  2:1414623/149‑1 (MQ=255)
                                           gTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGACAACCGCATTGATCGTCCCCTAttta                                                                                                        >  1:749670/1‑148 (MQ=255)
                                              gtgtTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCaaa                                                                                                     <  1:1053350/149‑1 (MQ=255)
                                                 ttCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGc                                                                                                  >  2:3379568/1‑149 (MQ=255)
                                                 ttCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGc                                                                                                  >  1:2650545/1‑149 (MQ=255)
                                                  tCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCt                                                                                                 <  2:587403/149‑1 (MQ=255)
                                                    cTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCtcg                                                                                               >  1:1669714/1‑149 (MQ=255)
                                                    cTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCtcg                                                                                               >  1:246881/1‑149 (MQ=255)
                                                       aaCACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTATAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCtcgtcg                                                                                            >  1:900229/1‑149 (MQ=255)
                                                       aaCACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCtcgtcg                                                                                            >  1:96833/1‑149 (MQ=255)
                                                        acacTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTcgc                                                                                           <  1:1915841/149‑1 (MQ=255)
                                                        acacTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTcgc                                                                                           >  1:3650493/1‑149 (MQ=255)
                                                            tataTTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGt                                                                                       >  1:2270586/1‑149 (MQ=255)
                                                            tataTTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGt                                                                                       <  1:440202/149‑1 (MQ=255)
                                                            tataTTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGt                                                                                       <  1:440118/149‑1 (MQ=255)
                                                                ttatCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTaaaa                                                                                   >  2:53110/1‑149 (MQ=255)
                                                                 tatCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAg                                                                                  <  1:1742000/149‑1 (MQ=255)
                                                                 tatCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAg                                                                                  <  1:1259405/149‑1 (MQ=255)
                                                                    cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGa                                                                               <  1:2211943/149‑1 (MQ=255)
                                                                       gctgctTATTTCCTTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGt                                                                            <  1:1288263/149‑1 (MQ=255)
                                                                       gctgctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGt                                                                            >  2:3742089/1‑149 (MQ=255)
                                                                        ctgctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGtt                                                                           <  1:2831774/149‑1 (MQ=255)
                                                                          gctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTtg                                                                         <  1:858936/149‑1 (MQ=255)
                                                                           ctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTtgt                                                                        >  1:1414473/1‑149 (MQ=255)
                                                                            ttATATCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCAATGATCGTCCCCTATTACAAACGCTCGACGCGGGTAAAAGCGACgataggg                                                                       >  2:1719918/1‑143 (MQ=255)
                                                                               tttCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAAt                                                                    >  2:345533/1‑149 (MQ=255)
                                                                                   cGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGt                                                                >  1:3370488/1‑149 (MQ=255)
                                                                                      aTTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaat                                                             <  1:99134/149‑1 (MQ=255)
                                                                                      aTTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaat                                                             >  2:3231648/1‑149 (MQ=255)
                                                                                      aTTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaat                                                             <  2:1498342/149‑1 (MQ=255)
                                                                                           tGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCt                                                        >  2:3135635/1‑149 (MQ=255)
                                                                                            ggCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCtt                                                       >  1:1097058/1‑149 (MQ=255)
                                                                                               aGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACa                                                    <  1:1030508/149‑1 (MQ=255)
                                                                                               aGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACa                                                    <  2:156202/149‑1 (MQ=255)
                                                                                                gTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTGTGTGAATCCGTAATAACCTTACAg                                                   >  1:760944/1‑149 (MQ=255)
                                                                                                    aaGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTaa                                               >  1:3917463/1‑149 (MQ=255)
                                                                                                    aaGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTaa                                               <  1:1498612/149‑1 (MQ=255)
                                                                                                     aGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAAc                                              <  1:2039317/149‑1 (MQ=255)
                                                                                                     aGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAAc                                              <  1:3727165/149‑1 (MQ=255)
                                                                                                     aGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAAc                                              <  2:2845344/149‑1 (MQ=255)
                                                                                                      gCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAAcc                                             >  2:2144122/1‑149 (MQ=255)
                                                                                                       cATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCt                                            >  1:2313731/1‑149 (MQ=255)
                                                                                                       cATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGGTTGTGAATCCGTAATAACCTTACAGTTAACCt                                            >  1:2446826/1‑149 (MQ=255)
                                                                                                           cGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCtgttg                                        <  2:3661347/149‑1 (MQ=255)
                                                                                                            gCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCtgttgt                                       <  2:1359351/149‑1 (MQ=255)
                                                                                                              tttttCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGGTAGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCg                                     >  2:1868645/1‑149 (MQ=255)
                                                                                                                  tCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTACAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTg                                 >  2:3291447/1‑149 (MQ=255)
                                                                                                                   cACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGc                                >  1:3826150/1‑149 (MQ=255)
                                                                                                                   cACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGc                                >  2:3521995/1‑149 (MQ=255)
                                                                                                                     cccGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGctc                              >  1:1967006/1‑149 (MQ=255)
                                                                                                                     cccGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGctc                              >  1:1828766/1‑149 (MQ=255)
                                                                                                                     cccGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGctc                              <  1:3293515/149‑1 (MQ=255)
                                                                                                                      ccGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGctct                             <  1:2535202/149‑1 (MQ=255)
                                                                                                                         tGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGa                          >  1:2631964/1‑149 (MQ=255)
                                                                                                                         tGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGa                          >  2:2771468/1‑149 (MQ=255)
                                                                                                                          ggAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGAt                         <  1:3576427/149‑1 (MQ=255)
                                                                                                                           gAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGAtt                        >  2:2448050/1‑149 (MQ=255)
                                                                                                                             aGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTaa                      <  2:3924883/149‑1 (MQ=255)
                                                                                                                             aGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTaa                      >  2:817115/1‑149 (MQ=255)
                                                                                                                             aGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTaa                      <  2:1914618/149‑1 (MQ=255)
                                                                                                                             aGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTaa                      <  2:1914078/149‑1 (MQ=255)
                                                                                                                                tGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACgg                   >  1:3068346/1‑149 (MQ=255)
                                                                                                                                 ggCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGa                  <  2:2650545/149‑1 (MQ=255)
                                                                                                                                 ggCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGa                  <  2:1766132/149‑1 (MQ=255)
                                                                                                                                      aGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGGTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGataata             >  1:1773176/1‑149 (MQ=255)
                                                                                                                                       gCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAg            <  1:1988337/149‑1 (MQ=255)
                                                                                                                                       gCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTAGTCGCCTGCTCTGGATTAACGGATAATAg            >  2:2083648/1‑149 (MQ=255)
                                                                                                                                         cGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAggc          <  1:2739514/149‑1 (MQ=255)
                                                                                                                                           ttCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAggcgg        <  1:3401278/149‑1 (MQ=255)
                                                                                                                                            tCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAggcggc       >  1:3040904/1‑149 (MQ=255)
                                                                                                                                              gCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCtt     <  2:1283694/149‑1 (MQ=255)
                                                                                                                                                 aGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCttttt  <  2:2594161/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TATTACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTT  >  CP009273/1699714‑1700007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑