Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 158,287 C→A 100% D78Y (GAC→TAC)  ligT ← 2'‑5' RNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273158,2870CA100.0% 887.2 / NA 264D78Y (GAC→TAC) ligT2'‑5' RNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (136/128);  total (136/128)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 268 total aligned reads displayed.

AATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGG  >  CP009273/158141‑158432
                                                                                                                                                  |                                                                                                                                                 
aaTATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGtaat                                                                                                                                                 >  2:3778599/1‑149 (MQ=255)
  tatGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGtaatca                                                                                                                                               >  1:499056/1‑149 (MQ=255)
   atGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCaa                                                                                                                                              <  2:2459835/149‑1 (MQ=255)
   atGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCaa                                                                                                                                              >  1:2039484/1‑149 (MQ=255)
   atGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCaa                                                                                                                                              >  2:2061964/1‑149 (MQ=255)
    tgtgGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAg                                                                                                                                             >  1:3030610/1‑149 (MQ=255)
         aTGAAACGGACGATTTCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATACTCCAGCTGGATAAAGCCGCGTGGCGGCTGACTCATCCCTAAACAAACAACACGCGAACGCAGCCAATGTCCCGCGTAATCAAGCGTGa                                                                                                                                        >  2:2391947/1‑149 (MQ=255)
           gAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAgt                                                                                                                                      >  2:3311587/1‑149 (MQ=255)
            aaaCGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAgtg                                                                                                                                     >  1:1326847/1‑149 (MQ=255)
            aaaCGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAgtg                                                                                                                                     <  1:3585433/149‑1 (MQ=255)
                 gACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGTGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGaaa                                                                                                                                >  2:1271989/1‑149 (MQ=255)
                   cGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAAcc                                                                                                                              >  1:3245536/1‑149 (MQ=255)
                    gATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCa                                                                                                                             >  2:475654/1‑149 (MQ=255)
                     aTTGCTTTGAAAACCACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAg                                                                                                                            <  2:1434101/149‑1 (MQ=255)
                     aTTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAg                                                                                                                            <  2:2997809/149‑1 (MQ=255)
                       tGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGt                                                                                                                          >  2:1779859/1‑149 (MQ=255)
                             gAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGa                                                                                                                    >  1:2827235/1‑149 (MQ=255)
                              aaaaCAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGaa                                                                                                                   >  2:272097/1‑149 (MQ=255)
                               aaaCAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAt                                                                                                                  <  2:141134/149‑1 (MQ=255)
                               aaaCAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAt                                                                                                                  <  2:2739839/149‑1 (MQ=255)
                                 acaacCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtcc                                                                                                                >  1:823925/1‑149 (MQ=255)
                                   aacCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtccgt                                                                                                              >  2:1717601/1‑149 (MQ=255)
                                    acCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtccgtc                                                                                                             <  1:714673/149‑1 (MQ=255)
                                     ccGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtccgtcc                                                                                                            >  2:2611395/1‑149 (MQ=255)
                                       gCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCgg                                                                                                          >  1:770444/1‑149 (MQ=255)
                                        cTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGc                                                                                                         <  2:2389536/149‑1 (MQ=255)
                                        cTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGc                                                                                                         <  2:1733623/149‑1 (MQ=255)
                                             ggCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTcaaa                                                                                                    >  2:2289742/1‑149 (MQ=255)
                                             ggCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTaaaa                                                                                                    <  1:2238816/149‑1 (MQ=255)
                                               catccTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTTGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCTTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAaga                                                                                                  <  1:1845423/146‑1 (MQ=255)
                                                aGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAagag                                                                                                 <  1:1568623/149‑1 (MQ=255)
                                                 tccTGTGAACGTAGCATATTCGAGAGCTGGATTAAGCCGCGTGGCTGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTACTGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAgaga                                                                                                <  1:73552/148‑1 (MQ=255)
                                                 gCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAgaga                                                                                                >  2:3922182/1‑149 (MQ=255)
                                                  ccTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGaa                                                                                               >  2:287098/1‑149 (MQ=255)
                                                  ccTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGaa                                                                                               <  1:2899391/149‑1 (MQ=255)
                                                    tgtgAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAg                                                                                             <  1:1346572/149‑1 (MQ=255)
                                                       gAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAgcgc                                                                                          <  2:2257249/149‑1 (MQ=255)
                                                       gAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAgcgc                                                                                          >  2:4048911/1‑149 (MQ=255)
                                                         aCGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCt                                                                                        <  2:3258610/149‑1 (MQ=255)
                                                         aCGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCt                                                                                        >  1:2615794/1‑149 (MQ=255)
                                                          cGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCtt                                                                                       >  1:1053381/1‑149 (MQ=255)
                                                          cGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCtt                                                                                       >  1:3108685/1‑149 (MQ=255)
                                                             aGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTtctc                                                                                    <  1:1716395/149‑1 (MQ=255)
                                                              gCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTctct                                                                                   <  1:2452105/149‑1 (MQ=255)
                                                              gCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTctct                                                                                   <  2:512530/149‑1 (MQ=255)
                                                               cATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCtt                                                                                  <  1:3416229/149‑1 (MQ=255)
                                                               cATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCtt                                                                                  <  2:3626318/149‑1 (MQ=255)
                                                                 tatTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTtc                                                                                <  1:1763670/149‑1 (MQ=255)
                                                                  atTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTtct                                                                               <  2:2677082/149‑1 (MQ=255)
                                                                        cAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGgcgc                                                                         <  2:1150803/149‑1 (MQ=255)
                                                                         aGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCt                                                                        >  2:1379076/1‑149 (MQ=255)
                                                                           cTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCa                                                                      >  2:1915755/1‑149 (MQ=255)
                                                                            tGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCAc                                                                     >  1:1134182/1‑149 (MQ=255)
                                                                            tGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCAc                                                                     <  2:3335212/149‑1 (MQ=255)
                                                                            tGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCAc                                                                     >  1:3524020/1‑149 (MQ=255)
                                                                            tGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCAc                                                                     <  1:2171323/149‑1 (MQ=255)
                                                                            tGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCAc                                                                     <  2:1295514/149‑1 (MQ=255)
                                                                            tGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCAc                                                                     <  2:484114/149‑1 (MQ=255)
                                                                             ggATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACt                                                                    >  2:867723/1‑149 (MQ=255)
                                                                             ggATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACt                                                                    >  2:532485/1‑149 (MQ=255)
                                                                             ggATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACt                                                                    >  2:2221998/1‑149 (MQ=255)
                                                                              gATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACtt                                                                   <  2:3272738/149‑1 (MQ=255)
                                                                                ttAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCg                                                                 >  1:3812717/1‑149 (MQ=255)
                                                                                ttAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCg                                                                 >  1:1923659/1‑149 (MQ=255)
                                                                                 tAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGc                                                                <  1:2661198/149‑1 (MQ=255)
                                                                                  aaGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTNAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGcc                                                               >  1:2084458/1‑149 (MQ=255)
                                                                                  aaGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGcc                                                               >  2:3980717/1‑149 (MQ=255)
                                                                                  aaGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGcc                                                               <  2:3449473/149‑1 (MQ=255)
                                                                                    gCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTa                                                             >  2:4070087/1‑149 (MQ=255)
                                                                                        cgTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTaaaaa                                                         <  1:366917/149‑1 (MQ=255)
                                                                                           ggcggcTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCAATTCGCCTAAAAATGc                                                      >  1:4103179/1‑149 (MQ=255)
                                                                                                 tGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGt                                                >  2:1212905/1‑149 (MQ=255)
                                                                                                     gCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCCGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGa                                            <  2:1852985/149‑1 (MQ=255)
                                                                                                        tCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGc                                         >  1:2273974/1‑149 (MQ=255)
                                                                                                          ccTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCaa                                       >  1:3043931/1‑149 (MQ=255)
                                                                                                           cTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCaaa                                      <  1:3120025/149‑1 (MQ=255)
                                                                                                           cTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCaaa                                      >  1:1523676/1‑149 (MQ=255)
                                                                                                           cTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCaaa                                      >  1:2712774/1‑149 (MQ=255)
                                                                                                           cTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCaaa                                      <  2:3492060/149‑1 (MQ=255)
                                                                                                            tAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAAt                                     <  2:3036765/149‑1 (MQ=255)
                                                                                                               ccacaccacaCGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTAt                                  >  1:3905875/1‑149 (MQ=255)
                                                                                                                cacaccacacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATc                                 <  1:2390654/149‑1 (MQ=255)
                                                                                                                   accacacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATcggc                              >  2:440122/1‑149 (MQ=255)
                                                                                                                     cacacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATcggcgg                            >  1:3686537/1‑149 (MQ=255)
                                                                                                                        acGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGa                         <  1:421661/149‑1 (MQ=255)
                                                                                                                        acGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGa                         >  1:1914787/1‑149 (MQ=255)
                                                                                                                        acGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGa                         <  1:3314421/149‑1 (MQ=255)
                                                                                                                          gcgAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGAcc                       <  2:945746/149‑1 (MQ=255)
                                                                                                                          gcgAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGAcc                       <  2:220986/149‑1 (MQ=255)
                                                                                                                          gcgAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGAcc                       <  1:4005539/149‑1 (MQ=255)
                                                                                                                           cgAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCg                      <  1:704690/149‑1 (MQ=255)
                                                                                                                             aaCGCAGCCATTGTGCGTCGTAATCAAGCGTGAGTGTTAAACCAGGTTGACGAATCGGTCCGGCTAAAATTGAAAGCGCCTTCTCTTTCTCGGTGCTCACTTCGCCTAAATATGCCAGAGTCAGATGCAAATTATCGGCGGCTACCGGa                    <  2:653309/149‑1 (MQ=255)
                                                                                                                                gCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGt                 >  1:1172457/1‑149 (MQ=255)
                                                                                                                                  aGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTcc               >  2:1820716/1‑149 (MQ=255)
                                                                                                                                    ccATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCg             >  2:316924/1‑149 (MQ=255)
                                                                                                                                    ccATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCg             >  1:219538/1‑149 (MQ=255)
                                                                                                                                     cATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGc            >  2:717576/1‑149 (MQ=255)
                                                                                                                                      aTTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGcc           <  2:3377952/149‑1 (MQ=255)
                                                                                                                                        tGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTc         >  1:980478/1‑149 (MQ=255)
                                                                                                                                           ccGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAgg      >  1:225275/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGG  >  CP009273/158141‑158432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑