BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,769,763	T→A	100%	intergenic (+62/‑81)	aroD → / → ydiF	3‑dehydroquinate dehydratase/putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,769,763	0	T	A	98.5%	737.1 / ‑0.3	223	intergenic (+62/‑81)	aroD/ydiF	3‑dehydroquinate dehydratase/putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit
Reads supporting (aligned to +/- strand): ref base T (1/3); new base A (137/82); total (138/85)
Fisher's exact test for biased strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 232 total aligned reads displayed.

TGGTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGT  >  CP009273/1769617‑1769908

tGGTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTaaa                                                                                                                                                 <  2:2834931/149‑1 (MQ=255)

  gTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTaaata                                                                                                                                               >  2:1228235/1‑149 (MQ=255)

  gTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTaaata                                                                                                                                               <  1:793114/149‑1 (MQ=255)

    gCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATtaaataaa                                                                                                                                             >  1:2009104/1‑149 (MQ=255)

      ggTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATaaaaa                                                                                                                                           <  1:1319172/149‑1 (MQ=255)

        tAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAata                                                                                                                                         <  1:1896602/149‑1 (MQ=255)

          aaaaaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATAtt                                                                                                                                       <  2:2438627/149‑1 (MQ=255)

           aaaaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATAttt                                                                                                                                      <  2:2004510/149‑1 (MQ=255)

            aaaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTc                                                                                                                                     <  1:593482/149‑1 (MQ=255)

            aaaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTc                                                                                                                                     <  1:1636573/149‑1 (MQ=255)

              aaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGt                                                                                                                                   <  1:2368777/149‑1 (MQ=255)

              aaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGt                                                                                                                                   >  1:2921488/1‑149 (MQ=255)

                 cGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCTGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAg                                                                                                                                <  1:3877538/149‑1 (MQ=255)

                 cGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAg                                                                                                                                <  2:1558600/149‑1 (MQ=255)

                   tCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGtc                                                                                                                              >  1:803255/1‑149 (MQ=255)

                    cTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGtct                                                                                                                             <  2:3408917/149‑1 (MQ=255)

                     tGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGtctc                                                                                                                            >  2:1941031/1‑149 (MQ=255)

                     tGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGtctc                                                                                                                            <  2:1844601/149‑1 (MQ=255)

                     tGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGtctc                                                                                                                            <  1:1091181/149‑1 (MQ=255)

                      gcgcCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTctct                                                                                                                           <  2:1486089/149‑1 (MQ=255)

                         ccAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCtgtg                                                                                                                        >  2:100977/1‑149 (MQ=255)

                          cAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTgc                                                                                                                       >  1:449961/1‑149 (MQ=255)

                            gggCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTgcgc                                                                                                                     <  1:3429852/149‑1 (MQ=255)

                              gCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTa                                                                                                                   >  1:3353189/1‑149 (MQ=255)

                               cAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTaa                                                                                                                  >  2:1739310/1‑149 (MQ=255)

                               cAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTaa                                                                                                                  >  1:1101026/1‑149 (MQ=255)

                                 aaTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAAtt                                                                                                                >  1:865373/1‑149 (MQ=255)

                                  aTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATtc                                                                                                               <  2:3996081/149‑1 (MQ=255)

                                    ctcGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATtctc                                                                                                             <  1:995899/149‑1 (MQ=255)

                                    ctcGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATtctc                                                                                                             <  1:376660/149‑1 (MQ=255)

                                     tcGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTCATTCTcc                                                                                                            >  1:2607518/1‑149 (MQ=255)

                                         tAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAATTAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAttt                                                                                                        >  2:3853165/1‑149 (MQ=255)

                                         tAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAttt                                                                                                        >  2:1904715/1‑149 (MQ=255)

                                         tAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAttt                                                                                                        >  1:618905/1‑149 (MQ=255)

                                         tAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAttt                                                                                                        >  1:2683768/1‑149 (MQ=255)

                                         tAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAttt                                                                                                        >  2:1903581/1‑149 (MQ=255)

                                          aaaTGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTg                                                                                                       <  2:2150949/149‑1 (MQ=255)

                                                 ttGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAgg                                                                                                >  1:3015956/1‑149 (MQ=255)

                                                     gcACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGaaa                                                                                            >  1:623102/1‑149 (MQ=255)

                                                      cACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGaaaa                                                                                           <  2:1342198/149‑1 (MQ=255)

                                                       aCGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAAt                                                                                          <  2:3584718/149‑1 (MQ=255)

                                                        cGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATc                                                                                         <  2:1186425/149‑1 (MQ=255)

                                                          gTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATcac                                                                                       >  1:3488454/1‑149 (MQ=255)

                                                          gTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATcac                                                                                       >  2:3559262/1‑149 (MQ=255)

                                                             ttAACTATTTCACCCCAGGCAGACGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATc                                                                                    <  2:4083991/149‑1 (MQ=255)

                                                               aaCTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTg                                                                                  >  1:1289327/1‑149 (MQ=255)

                                                                 cTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGaa                                                                                <  1:1442234/149‑1 (MQ=255)

                                                                 cTATATTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGaa                                                                                <  1:1442139/149‑1 (MQ=255)

                                                                    ttttACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCa                                                                             >  1:2862018/1‑149 (MQ=255)

                                                                     tttACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAg                                                                            >  1:3393606/1‑149 (MQ=255)

                                                                      ttACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTCGGGAAAATCACATCTGACTCAgg                                                                           >  2:3736293/1‑149 (MQ=255)

                                                                        acacCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGaa                                                                         <  2:3174992/149‑1 (MQ=255)

                                                                           ccAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTa                                                                      >  1:920527/1‑149 (MQ=255)

                                                                           ccAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTa                                                                      <  1:1230117/149‑1 (MQ=255)

                                                                            cAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTaa                                                                     >  2:1393298/1‑149 (MQ=255)

                                                                             aGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAAc                                                                    <  1:1654141/149‑1 (MQ=255)

                                                                                cATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAAt                                                                 <  2:1289327/149‑1 (MQ=255)

                                                                                 aTAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAATAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATg                                                                >  2:2532515/1‑149 (MQ=255)

                                                                                 aTAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATg                                                                >  1:1212028/1‑149 (MQ=255)

                                                                                  tAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGa                                                               <  1:1359342/149‑1 (MQ=255)

                                                                                  tAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGa                                                               >  1:1774858/1‑149 (MQ=255)

                                                                                  tAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGa                                                               >  1:2138546/1‑149 (MQ=255)

                                                                                   aaGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGATAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGaa                                                              >  2:137995/1‑149 (MQ=255)

                                                                                     gCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAAc                                                            >  1:3130282/1‑149 (MQ=255)

                                                                                        ataataTTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTg                                                         >  2:3907959/1‑149 (MQ=255)

                                                                                         taataTTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGt                                                        >  2:1096497/1‑149 (MQ=255)

                                                                                         taataTTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGt                                                        <  2:1672370/149‑1 (MQ=255)

                                                                                                     gggAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCg                                            >  1:4011455/1‑149 (MQ=255)

                                                                                                     gggAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCg                                            >  1:1078526/1‑149 (MQ=255)

                                                                                                       gAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTa                                          >  1:2267270/1‑149 (MQ=255)

                                                                                                          cacCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTa                                       >  2:784400/1‑149 (MQ=255)

                                                                                                           acCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTaa                                      >  2:1215942/1‑149 (MQ=255)

                                                                                                            cccTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAAt                                     >  2:770477/1‑149 (MQ=255)

                                                                                                              cTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATgg                                   <  2:2911964/149‑1 (MQ=255)

                                                                                                              cTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATgg                                   <  2:2235458/149‑1 (MQ=255)

                                                                                                              cTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATgg                                   <  2:2235500/149‑1 (MQ=255)

                                                                                                                 cccGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACg                                >  2:4095809/1‑149 (MQ=255)

                                                                                                                 cccGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACg                                >  1:1526502/1‑149 (MQ=255)

                                                                                                                       gAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGcc                          <  1:3748231/149‑1 (MQ=255)

                                                                                                                          cTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGt                       >  2:3894426/1‑149 (MQ=255)

                                                                                                                           tAAAAAATATATTCACTCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTc                      <  1:1814694/149‑1 (MQ=255)

                                                                                                                           tAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTc                      >  2:1561123/1‑149 (MQ=255)

                                                                                                                           tAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTc                      >  1:1992398/1‑149 (MQ=255)

                                                                                                                            aaaaaaTATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTcc                     >  2:1754840/1‑149 (MQ=255)

                                                                                                                            aaaaaaTATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTcc                     >  1:2497665/1‑149 (MQ=255)

                                                                                                                            aaaaaaTATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTcc                     <  1:4055241/149‑1 (MQ=255)

                                                                                                                               aaaTATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGt                  >  1:2286341/1‑149 (MQ=255)

                                                                                                                                aaTATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTc                 >  2:2881792/1‑149 (MQ=255)

                                                                                                                                aaTATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTc                 >  2:515691/1‑149 (MQ=255)

                                                                                                                                 atatatTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCg                >  2:1112401/1‑149 (MQ=255)

                                                                                                                                 atatatTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCg                >  2:1321629/1‑149 (MQ=255)

                                                                                                                                  tatatTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCgg               >  2:3004807/1‑149 (MQ=255)

                                                                                                                                  tatatTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCgg               >  2:2439508/1‑149 (MQ=255)

                                                                                                                                  tatatTCAATCGTATTAAATAAAAATATTACGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTATTGGACGAGTGCCGGTCCTGTCgg               >  2:944311/1‑149 (MQ=255)

                                                                                                                                    tatTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGca             <  2:865373/149‑1 (MQ=255)

                                                                                                                                      ttCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGTGAAAATCACATCTGAATCAGGAATTAACAATGCAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGcaca           <  1:2527334/149‑1 (MQ=255)

                                                                                                                                      ttCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGcaca           <  1:3105967/149‑1 (MQ=255)

                                                                                                                                        cAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAgg         >  2:202975/1‑149 (MQ=255)

                                                                                                                                          aTCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGaa       <  2:3352566/149‑1 (MQ=255)

                                                                                                                                               aTTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGt  >  2:754164/1‑149 (MQ=255)

TGGTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGGT  >  CP009273/1769617‑1769908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`