Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,803,109 C→T 100% A128T (GCC→ACC)  yniB ← putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,803,1090CT99.0% 950.5 / ‑0.4 286A128T (GCC→ACC) yniBputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (165/118);  total (167/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 288 total aligned reads displayed.

CCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAA  >  CP009273/1802963‑1803253
                                                                                                                                                  |                                                                                                                                                
cccAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGttt                                                                                                                                                <  1:3754848/149‑1 (MQ=255)
  cAATCATAGAAAATAACACATAAAATTCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAAAAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTtttt                                                                                                                                              <  1:2825100/149‑1 (MQ=255)
  cAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTtttt                                                                                                                                              >  2:1978201/1‑149 (MQ=255)
    aGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTc                                                                                                                                            >  1:581489/1‑149 (MQ=255)
       agagAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTCGCCCCTCTTCCCCTTTGGTTTTTTCCAg                                                                                                                                         >  1:3571055/1‑149 (MQ=255)
          gaAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAAt                                                                                                                                      <  1:1708413/149‑1 (MQ=255)
              aGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGt                                                                                                                                  <  2:3885288/149‑1 (MQ=255)
               gAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGtt                                                                                                                                 <  1:805596/149‑1 (MQ=255)
               gAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGtt                                                                                                                                 >  2:3140428/1‑149 (MQ=255)
                aaCACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTg                                                                                                                                <  1:3310561/149‑1 (MQ=255)
                aaCACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTg                                                                                                                                <  1:1551107/149‑1 (MQ=255)
                 acacATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGa                                                                                                                               >  2:1305339/1‑149 (MQ=255)
                    caTAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCt                                                                                                                            >  1:1199468/1‑149 (MQ=255)
                        aCCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCaa                                                                                                                        <  1:3109601/149‑1 (MQ=255)
                           tGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAacac                                                                                                                     <  1:2302341/149‑1 (MQ=255)
                            gcagcaATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACAcc                                                                                                                    <  1:88204/149‑1 (MQ=255)
                              agcaATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCtt                                                                                                                  >  1:3008822/1‑149 (MQ=255)
                              agcaATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCtt                                                                                                                  >  2:2042845/1‑149 (MQ=255)
                               gcaATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTc                                                                                                                 <  2:1747824/149‑1 (MQ=255)
                               gaaaTACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTc                                                                                                                 <  1:2103615/147‑1 (MQ=255)
                                 aaTACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTccc                                                                                                               >  2:2371613/1‑149 (MQ=255)
                                   tACAAATAACCGTCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGc                                                                                                             <  2:2602772/149‑1 (MQ=255)
                                      aaaTAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaag                                                                                                          >  2:869259/1‑149 (MQ=255)
                                       aaTAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaaga                                                                                                         <  2:3597240/149‑1 (MQ=255)
                                        aTAACCGGCAGGATATACAGTGAGAAAAACTTCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaagaa                                                                                                        >  2:566629/1‑149 (MQ=255)
                                         tAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaagaag                                                                                                       >  2:2157531/1‑149 (MQ=255)
                                          aaCCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgc                                                                                                      <  1:484447/149‑1 (MQ=255)
                                           aCCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcg                                                                                                     >  2:1494996/1‑149 (MQ=255)
                                           aCCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcg                                                                                                     >  1:1050249/1‑149 (MQ=255)
                                           aCCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcg                                                                                                     >  2:2980787/1‑149 (MQ=255)
                                            ccGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcgc                                                                                                    >  2:2759431/1‑149 (MQ=255)
                                               gCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGcc                                                                                                 <  2:2516896/149‑1 (MQ=255)
                                               gCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGcc                                                                                                 >  2:310512/1‑149 (MQ=255)
                                               gCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGcc                                                                                                 <  2:3339167/149‑1 (MQ=255)
                                                cAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCt                                                                                                >  1:947948/1‑149 (MQ=255)
                                                 aGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTg                                                                                               >  2:1601341/1‑149 (MQ=255)
                                                  ggATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTgg                                                                                              >  1:2459008/1‑149 (MQ=255)
                                                   gATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGc                                                                                             >  2:72471/1‑149 (MQ=255)
                                                    atatACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCg                                                                                            >  1:1125794/1‑149 (MQ=255)
                                                     tataCAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGa                                                                                           >  1:2160614/1‑149 (MQ=255)
                                                      ataCAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGAGTTAGCCCCTCTTACGCATAGGTTTTTTCCAGAATGAGGTGATCTTCAAAACCATCCCGCAAGAAGCGCGCCTGGCGAc                                                                                          >  1:1040123/1‑149 (MQ=255)
                                                       taCAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACt                                                                                         >  1:3855386/1‑149 (MQ=255)
                                                       taCAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGACTGGCGACt                                                                                         <  1:3276808/149‑1 (MQ=255)
                                                           gTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATc                                                                                     <  1:1374752/149‑1 (MQ=255)
                                                           gTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATc                                                                                     <  1:2880015/149‑1 (MQ=255)
                                                           gTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATc                                                                                     <  1:65250/149‑1 (MQ=255)
                                                            tGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATcc                                                                                    >  2:1267632/1‑149 (MQ=255)
                                                             gagaAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCg                                                                                   >  1:3517248/1‑149 (MQ=255)
                                                               gaAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCggg                                                                                 >  1:2955393/1‑149 (MQ=255)
                                                                aaaaaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGc                                                                                <  1:3489797/149‑1 (MQ=255)
                                                                  aaaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCAc                                                                              >  1:1393342/1‑149 (MQ=255)
                                                                     cTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCgg                                                                           >  1:4069980/1‑149 (MQ=255)
                                                                       gCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGaa                                                                         <  1:1479721/149‑1 (MQ=255)
                                                                          ggAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATcc                                                                      >  1:1286168/1‑149 (MQ=255)
                                                                           gAAAATAGTATGGAGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGACTGAGTTGATCTTCAACACCTTCCCGCACGAAGCGCGCCTGGCGACTCATCCGGGCACCGGACTCCt                                                                     >  2:2631977/1‑149 (MQ=255)
                                                                             aaaTAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGc                                                                   >  2:93556/1‑149 (MQ=255)
                                                                              aaTAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCa                                                                  >  1:1499571/1‑149 (MQ=255)
                                                                                        tGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTcc                                                        >  2:2582736/1‑149 (MQ=255)
                                                                                           ggAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGAc                                                     <  1:3393372/149‑1 (MQ=255)
                                                                                            gAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACa                                                    >  2:30291/1‑149 (MQ=255)
                                                                                            gAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACa                                                    >  2:2027565/1‑149 (MQ=255)
                                                                                                  caaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATc                                              <  2:3516934/149‑1 (MQ=255)
                                                                                                   aaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCa                                             <  1:4037454/149‑1 (MQ=255)
                                                                                                    aTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAg                                            >  1:299869/1‑149 (MQ=255)
                                                                                                    aTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAg                                            <  2:2325778/149‑1 (MQ=255)
                                                                                                    aTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCAGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAg                                            >  2:4081020/1‑149 (MQ=255)
                                                                                                     tACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAgg                                           >  2:185600/1‑149 (MQ=255)
                                                                                                      aCGGGACTCAAGTTGTTCACGCGTTAGCCCCTCGTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGa                                          <  1:2707933/149‑1 (MQ=255)
                                                                                                        gggACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATg                                        >  2:1198696/1‑149 (MQ=255)
                                                                                                         ggACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCTGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGa                                       >  2:1204871/1‑149 (MQ=255)
                                                                                                          gACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGaa                                      >  1:751059/1‑149 (MQ=255)
                                                                                                                 tttGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCAcc                               <  2:2800795/149‑1 (MQ=255)
                                                                                                                 tttGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCAcc                               <  1:3356735/149‑1 (MQ=255)
                                                                                                                 tttGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCAcc                               >  2:167454/1‑149 (MQ=255)
                                                                                                                  ttGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCAccc                              <  1:677711/149‑1 (MQ=255)
                                                                                                                   tGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCa                             <  2:1535963/149‑1 (MQ=255)
                                                                                                                   tGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCa                             <  2:1933463/149‑1 (MQ=255)
                                                                                                                     ttCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGa                           >  1:3179013/1‑149 (MQ=255)
                                                                                                                      tCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGaa                          <  2:2857466/149‑1 (MQ=255)
                                                                                                                       cACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAAc                         >  1:189863/1‑149 (MQ=255)
                                                                                                                            gTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAAc                    <  1:3985129/149‑1 (MQ=255)
                                                                                                                            gTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAAc                    >  2:2742932/1‑149 (MQ=255)
                                                                                                                            gTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAAc                    <  2:1527517/149‑1 (MQ=255)
                                                                                                                              tAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGt                  <  2:338904/149‑1 (MQ=255)
                                                                                                                              tAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGt                  <  2:2411156/149‑1 (MQ=255)
                                                                                                                               aGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCCGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGtt                 >  1:2203937/1‑149 (MQ=255)
                                                                                                                               aGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGtt                 <  1:2143454/149‑1 (MQ=255)
                                                                                                                               aGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGtt                 <  1:1267632/149‑1 (MQ=255)
                                                                                                                                gCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTc                >  2:2245603/1‑149 (MQ=255)
                                                                                                                                  cccTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCAc              >  1:3418154/1‑149 (MQ=255)
                                                                                                                                  cccTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCAc              >  1:1677758/1‑149 (MQ=255)
                                                                                                                                      ctTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGcca          <  2:4114699/149‑1 (MQ=255)
                                                                                                                                       ttCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccac         >  2:3624963/1‑149 (MQ=255)
                                                                                                                                           ccTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTTCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCAtt     <  2:3952101/149‑1 (MQ=255)
                                                                                                                                           ccTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCAtt     <  2:1704785/149‑1 (MQ=255)
                                                                                                                                             tttGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCCCCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACCTAACGTTCACGccacccgtca   >  2:2289254/1‑144 (MQ=255)
                                                                                                                                             tttGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTaa   >  1:1962000/1‑149 (MQ=255)
                                                                                                                                             tttGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTaa   <  2:614583/149‑1 (MQ=255)
                                                                                                                                             tttGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTaa   >  1:364983/1‑149 (MQ=255)
                                                                                                                                              ttGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTaaa  >  1:3930224/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAA  >  CP009273/1802963‑1803253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑