Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,812,514 C→A 100% D82Y (GAT→TAT)  chbF ← phospho‑chitobiase; general 6‑phospho‑beta‑glucosidase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,812,5140CA99.1% 702.4 / ‑1.8 212D82Y (GAT→TAT) chbFphospho‑chitobiase; general 6‑phospho‑beta‑glucosidase activity
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (95/115);  total (96/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 212 total aligned reads displayed.

AAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATCAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTG  >  CP009273/1812368‑1812659
                                                                                                                                                  |                                                                                                                                                 
aaaaaTCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAg                                                                                                                                                 >  1:2178185/1‑149 (MQ=255)
  aaaTCACCTGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCGAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCa                                                                                                                                               <  2:247167/149‑1 (MQ=255)
  aaaTCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCa                                                                                                                                               <  2:1587424/149‑1 (MQ=255)
  aaaTCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCa                                                                                                                                               <  1:3416/149‑1 (MQ=255)
    aTCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATc                                                                                                                                             <  2:3125779/149‑1 (MQ=255)
     tCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCt                                                                                                                                            >  1:1855457/1‑149 (MQ=255)
      cACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCtt                                                                                                                                           <  1:2033566/149‑1 (MQ=255)
      cACCGGAAGGGTACGAAGACCTTAAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCtt                                                                                                                                           <  1:392189/149‑1 (MQ=255)
        ccGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTc                                                                                                                                         >  1:907148/1‑149 (MQ=255)
          ggAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTTATGTAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCaa                                                                                                                                       <  2:2275911/149‑1 (MQ=255)
          ggAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATCAGCATCTTTCaa                                                                                                                                       <  1:3807565/149‑1 (MQ=255)
           gAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAAt                                                                                                                                      >  2:855904/1‑149 (MQ=255)
           gAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAAt                                                                                                                                      <  2:2649130/149‑1 (MQ=255)
            aaTGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATg                                                                                                                                     >  2:3728794/1‑149 (MQ=255)
            aaTGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATg                                                                                                                                     >  1:1086620/1‑149 (MQ=255)
             aTGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGc                                                                                                                                    <  1:1465506/149‑1 (MQ=255)
             aTGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGc                                                                                                                                    >  2:940482/1‑149 (MQ=255)
               ggTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCtt                                                                                                                                  >  1:3154552/1‑149 (MQ=255)
                gTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTc                                                                                                                                 >  1:3374078/1‑149 (MQ=255)
                   cGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTcgcg                                                                                                                              >  1:733587/1‑149 (MQ=255)
                    gCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCgcgg                                                                                                                             >  1:1749461/1‑149 (MQ=255)
                       gACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGa                                                                                                                          <  2:3647937/149‑1 (MQ=255)
                        aCCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGAt                                                                                                                         >  1:1107830/1‑149 (MQ=255)
                         ccTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATc                                                                                                                        <  2:3242718/149‑1 (MQ=255)
                          cTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCGTCGCGGCGATcc                                                                                                                       >  1:2161723/1‑149 (MQ=255)
                           tttAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCa                                                                                                                      >  1:3782474/1‑149 (MQ=255)
                           tttAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCa                                                                                                                      >  1:1850186/1‑149 (MQ=255)
                               aacaaacCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGt                                                                                                                  <  2:716411/149‑1 (MQ=255)
                               aacaaacCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGt                                                                                                                  <  2:1513172/149‑1 (MQ=255)
                               aacaaacCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGt                                                                                                                  >  2:1267601/1‑149 (MQ=255)
                                acaaacCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGtt                                                                                                                 >  2:4034374/1‑149 (MQ=255)
                                    acCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGGAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTtat                                                                                                             >  1:44938/1‑149 (MQ=255)
                                      cgcccgcGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATaa                                                                                                           <  1:642178/149‑1 (MQ=255)
                                           gcgcCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCtt                                                                                                      >  1:3985289/1‑149 (MQ=255)
                                            cgcCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTc                                                                                                     <  1:3612709/149‑1 (MQ=255)
                                              ccGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCAt                                                                                                   <  1:2829275/149‑1 (MQ=255)
                                              ccGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCAt                                                                                                   <  2:3804171/149‑1 (MQ=255)
                                                gTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCACCGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGATTTATAAAGCTTCATCg                                                                                                 >  2:2777563/1‑149 (MQ=255)
                                                gTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCg                                                                                                 >  2:646879/1‑149 (MQ=255)
                                                gTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCg                                                                                                 <  1:1194021/149‑1 (MQ=255)
                                                 ttGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCgg                                                                                                >  2:2085516/1‑149 (MQ=255)
                                                 ttGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCgg                                                                                                <  2:3841359/149‑1 (MQ=255)
                                                        ccTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCa                                                                                         <  2:3842556/149‑1 (MQ=255)
                                                         cTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAg                                                                                        >  1:2929968/1‑149 (MQ=255)
                                                           gACCAAGATAACCATGACTTAATGGAATACGTTCATCCAATTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCg                                                                                      >  2:1048994/1‑149 (MQ=255)
                                                            aCCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGt                                                                                     >  1:452538/1‑149 (MQ=255)
                                                             ccAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGtt                                                                                    >  2:1861347/1‑149 (MQ=255)
                                                             ccAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGtt                                                                                    >  1:109351/1‑149 (MQ=255)
                                                              cAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTa                                                                                   <  1:1207707/149‑1 (MQ=255)
                                                              cAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTa                                                                                   <  2:1224886/149‑1 (MQ=255)
                                                              cAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTa                                                                                   <  1:3254576/149‑1 (MQ=255)
                                                               aaGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTat                                                                                  >  2:2890537/1‑149 (MQ=255)
                                                                     aCCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTatcaatca                                                                            <  2:848356/149‑1 (MQ=255)
                                                                     aCCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTatcaatca                                                                            <  1:2661712/149‑1 (MQ=255)
                                                                      ccATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAatcat                                                                           >  2:4095914/1‑149 (MQ=255)
                                                                      ccATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAatcat                                                                           >  2:287552/1‑149 (MQ=255)
                                                                        aTGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATcc                                                                         <  2:3456929/149‑1 (MQ=255)
                                                                         tGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTACCGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCg                                                                        >  2:2933424/1‑149 (MQ=255)
                                                                          gACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGt                                                                       >  1:16469/1‑149 (MQ=255)
                                                                             ttAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTgg                                                                    <  1:708615/149‑1 (MQ=255)
                                                                             ttAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTgg                                                                    >  2:3190998/1‑149 (MQ=255)
                                                                             ttAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTgg                                                                    <  1:3705952/149‑1 (MQ=255)
                                                                                aTGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCag                                                                 >  2:913315/1‑149 (MQ=255)
                                                                                aTGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCag                                                                 >  1:3544716/1‑149 (MQ=255)
                                                                                aTGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCag                                                                 >  2:2970964/1‑149 (MQ=255)
                                                                                   gAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAgaga                                                              <  1:3571857/149‑1 (MQ=255)
                                                                                    aaTACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGAt                                                             <  2:1855457/149‑1 (MQ=255)
                                                                                    aaTACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGAt                                                             <  2:2811950/149‑1 (MQ=255)
                                                                                    aaTACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTACCAATCATCCGTTGGCAGAGAt                                                             <  2:3593713/149‑1 (MQ=255)
                                                                                     aTACGTTCATCCAGTTCACGCGCCGGTACTTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATc                                                            >  1:116430/1‑149 (MQ=255)
                                                                                      tACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCg                                                           >  2:3200542/1‑149 (MQ=255)
                                                                                      tACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCg                                                           >  2:3200758/1‑149 (MQ=255)
                                                                                         gTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGaaa                                                        >  2:1113148/1‑149 (MQ=255)
                                                                                         gTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGaaa                                                        <  2:1574164/149‑1 (MQ=255)
                                                                                          ttCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGaaaa                                                       <  1:2937517/149‑1 (MQ=255)
                                                                                          ttCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGaaaa                                                       >  2:122244/1‑149 (MQ=255)
                                                                                          ttCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGaaaa                                                       >  1:31886/1‑149 (MQ=255)
                                                                                           tCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAaat                                                      <  2:1629026/149‑1 (MQ=255)
                                                                                               ccAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAaataatn                                                  <  1:1267600/149‑2 (MQ=255)
                                                                                                  gTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATcc                                               <  2:2624039/149‑1 (MQ=255)
                                                                                                   ttCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTTACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCCTCCGTTGGCAGAGATCGAAAATAATATCCa                                              <  2:1127113/149‑1 (MQ=255)
                                                                                                   ttCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCa                                              <  1:940504/149‑1 (MQ=255)
                                                                                                        gcgcCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTc                                         >  1:2715370/1‑149 (MQ=255)
                                                                                                                aaTTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGTCAGAGATCGAAAATAATATCCAGTTTCGGTTTacc                                 <  2:1098167/149‑1 (MQ=255)
                                                                                                                 aTTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTacca                                <  1:3142180/149‑1 (MQ=255)
                                                                                                                     gCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCtt                            >  1:402594/1‑149 (MQ=255)
                                                                                                                     gCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCtt                            <  2:1778350/149‑1 (MQ=255)
                                                                                                                     gCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCtt                            <  2:3782474/149‑1 (MQ=255)
                                                                                                                       cAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCg                          >  2:2553228/1‑149 (MQ=255)
                                                                                                                         aCGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGAc                        >  1:3758378/1‑149 (MQ=255)
                                                                                                                           gcgcAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACAt                      >  2:1376729/1‑149 (MQ=255)
                                                                                                                               aGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATccac                  >  1:3015/1‑149 (MQ=255)
                                                                                                                                gTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATccacc                 >  1:1311080/1‑149 (MQ=255)
                                                                                                                                  tGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAg               <  2:2175089/149‑1 (MQ=255)
                                                                                                                                    ggTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGcc             >  2:3623345/1‑149 (MQ=255)
                                                                                                                                           aCGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTc      <  2:377611/149‑1 (MQ=255)
                                                                                                                                           aCGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTc      <  2:2447821/149‑1 (MQ=255)
                                                                                                                                             gAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGc    <  1:2613105/149‑1 (MQ=255)
                                                                                                                                              aaaTAAGCATCTTTCAATGCGTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCt   <  2:1940035/149‑1 (MQ=255)
                                                                                                                                               aaTAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTg  >  2:3392958/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATCAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTG  >  CP009273/1812368‑1812659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑