Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,820,888 C→A 100% C98F (TGC→TTC)  astE ← succinylglutamate desuccinylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,820,8880CA99.0% 643.1 / ‑2.0 195C98F (TGC→TTC) astEsuccinylglutamate desuccinylase
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (97/96);  total (99/96)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 198 total aligned reads displayed.

GTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGC  >  CP009273/1820742‑1821033
                                                                                                                                                  |                                                                                                                                                 
gTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGaaa                                                                                                                                                 <  2:1481872/149‑1 (MQ=255)
gTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGaaa                                                                                                                                                 >  1:1817670/1‑149 (MQ=255)
   gATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGAAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATaa                                                                                                                                              <  1:2887786/149‑1 (MQ=255)
    aTCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAAc                                                                                                                                             <  1:3017556/149‑1 (MQ=255)
    aTCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAAc                                                                                                                                             <  2:159343/149‑1 (MQ=255)
      cAAGGTGCCAGCGAACAGATTCTATGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGAAAGGTTTCTCCGCTTACAGCAAAAAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGt                                                                                                                                           >  2:3369275/1‑149 (MQ=255)
       aaGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTCCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGtt                                                                                                                                          <  2:2627381/149‑1 (MQ=255)
         ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTc                                                                                                                                        <  2:562134/149‑1 (MQ=255)
         ggTGCCAGCGCACAGATTCTTTGCCCTGGTAATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTc                                                                                                                                        <  1:2545433/149‑1 (MQ=255)
             ccAGCGCACATATCCTTTGCCCTGGTCATAAAAATCATCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCtt                                                                                                                                    <  2:2848714/149‑1 (MQ=255)
             ccAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCtt                                                                                                                                    <  1:1730880/149‑1 (MQ=255)
                gcgcACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCt                                                                                                                                 <  1:1018295/149‑1 (MQ=255)
                  gcACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTc                                                                                                                               <  2:1821215/149‑1 (MQ=255)
                       gATTCTTTGCCCTGGTCATAAAAATCTTCCCGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAgcgc                                                                                                                          <  1:1206111/149‑1 (MQ=255)
                       gATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAgcgc                                                                                                                          >  2:1948295/1‑149 (MQ=255)
                       gATTCTTTGCCCTGGTCAAAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAgcgc                                                                                                                          <  1:1777992/149‑1 (MQ=255)
                         ttCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCag                                                                                                                        <  2:1934276/149‑1 (MQ=255)
                         ttCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCACCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCag                                                                                                                        >  1:1673268/1‑149 (MQ=255)
                            tttGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCaggag                                                                                                                     >  1:1624997/1‑149 (MQ=255)
                             ttGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCaggagg                                                                                                                    <  1:558293/149‑1 (MQ=255)
                                cccTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGAtt                                                                                                                 <  2:3996885/149‑1 (MQ=255)
                                cccTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGAtt                                                                                                                 <  2:669830/149‑1 (MQ=255)
                                    ggTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCg                                                                                                             <  1:1558893/149‑1 (MQ=255)
                                     gTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCACCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGa                                                                                                            <  2:1673268/149‑1 (MQ=255)
                                        aTAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGa                                                                                                         >  1:2616991/1‑149 (MQ=255)
                                         tAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGAt                                                                                                        >  2:4107137/1‑149 (MQ=255)
                                         tAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGAt                                                                                                        <  2:3711622/149‑1 (MQ=255)
                                         tAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGAt                                                                                                        >  2:219867/1‑149 (MQ=255)
                                          aaaaaTCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCGGCGCAGGAGGATTCCCGAGGATc                                                                                                       <  1:1163047/149‑1 (MQ=255)
                                          aaaaaTCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATc                                                                                                       <  1:3854136/149‑1 (MQ=255)
                                           aaaaTCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCa                                                                                                      >  2:1269556/1‑149 (MQ=255)
                                           aaaaTCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCa                                                                                                      >  2:2740659/1‑149 (MQ=255)
                                            aaaTCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCAc                                                                                                     <  1:2215693/149‑1 (MQ=255)
                                             aaTCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCAcc                                                                                                    <  1:979465/149‑1 (MQ=255)
                                              aTCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTATCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCa                                                                                                   <  2:3797816/149‑1 (MQ=255)
                                                 ttCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCa                                                                                                >  2:815697/1‑149 (MQ=255)
                                                   ccAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAAc                                                                                              <  1:3308913/149‑1 (MQ=255)
                                                     aGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCg                                                                                            >  2:2285230/1‑149 (MQ=255)
                                                         aCTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCCGCAACCGCCaa                                                                                        >  2:1050236/1‑149 (MQ=255)
                                                         aCTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCaa                                                                                        >  2:1052249/1‑149 (MQ=255)
                                                          cTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAAc                                                                                       >  2:273806/1‑149 (MQ=255)
                                                          cTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAAc                                                                                       >  2:1002292/1‑149 (MQ=255)
                                                              tCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTaa                                                                                   <  1:274257/149‑1 (MQ=255)
                                                              tCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTaa                                                                                   <  1:2273521/149‑1 (MQ=255)
                                                                cAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACg                                                                                 >  1:848396/1‑149 (MQ=255)
                                                                 aGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACgg                                                                                >  2:1434504/1‑149 (MQ=255)
                                                                  gTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCACCGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACggg                                                                               <  2:3234886/149‑1 (MQ=255)
                                                                  gTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACggg                                                                               <  2:3234768/149‑1 (MQ=255)
                                                                    tCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGAt                                                                             >  2:4065653/1‑149 (MQ=255)
                                                                      gcgcgcCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGAtct                                                                           >  1:4113485/1‑149 (MQ=255)
                                                                       cgcgcCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGAtctc                                                                          <  2:814311/149‑1 (MQ=255)
                                                                               aCAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTgag                                                                  <  1:691406/149‑1 (MQ=255)
                                                                                cAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTgaga                                                                 <  2:1653127/149‑1 (MQ=255)
                                                                                 aGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGat                                                                >  1:3643935/1‑149 (MQ=255)
                                                                                  ggTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGata                                                               <  1:814284/149‑1 (MQ=255)
                                                                                  ggTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGAAACGGGATCTCGCCGTGAGata                                                               >  2:1992497/1‑149 (MQ=255)
                                                                                    tttCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATc                                                             >  2:2526727/1‑149 (MQ=255)
                                                                                     ttCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATct                                                            <  2:3905994/149‑2 (MQ=255)
                                                                                      tctcCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATcgc                                                           <  2:53306/149‑1 (MQ=255)
                                                                                      tctcCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATcgc                                                           <  2:2317966/149‑1 (MQ=255)
                                                                                       ctcCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATcgcg                                                          >  2:3524269/1‑149 (MQ=255)
                                                                                             tttCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGc                                                    <  1:3196201/149‑1 (MQ=255)
                                                                                                    aaaTAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGt                                             >  2:1745389/1‑149 (MQ=255)
                                                                                                      aTAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTcc                                           <  2:3952851/149‑1 (MQ=255)
                                                                                                       tAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCa                                          <  2:2812793/149‑1 (MQ=255)
                                                                                                         gCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCGACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGc                                        <  2:888874/149‑1 (MQ=255)
                                                                                                              cAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCAtctc                                   >  1:3333111/1‑149 (MQ=255)
                                                                                                                aCGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCca                                 <  2:699463/149‑1 (MQ=255)
                                                                                                                 cGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCcac                                >  2:2392253/1‑149 (MQ=255)
                                                                                                                    ccGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAgg                             >  1:3763563/1‑149 (MQ=255)
                                                                                                                     cGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGt                            <  1:3685478/149‑1 (MQ=255)
                                                                                                                      gCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTg                           >  2:2124143/1‑149 (MQ=255)
                                                                                                                      gCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTg                           >  1:4087344/1‑149 (MQ=255)
                                                                                                                       ccAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGc                          <  1:3183280/149‑1 (MQ=255)
                                                                                                                        cAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGcc                         >  2:1399420/1‑149 (MQ=255)
                                                                                                                        cAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGcc                         >  1:1631202/1‑149 (MQ=255)
                                                                                                                        cAAACATTCGAATCATATCGCTATGGAAATAACGTATCCCATGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTCAGAGATCCCGCCAAGCAACGCCTCCAGCATCTACACAGGTGcc                         >  2:3447495/1‑149 (MQ=255)
                                                                                                                           aCATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGtc                      <  1:936491/149‑1 (MQ=255)
                                                                                                                            cATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGtct                     >  1:1609429/1‑149 (MQ=255)
                                                                                                                            cATTCGATTCAGATCGCTATGGAAATAACGTGTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCCTCTCCACAGGTGACGtct                     <  2:2142192/149‑1 (MQ=255)
                                                                                                                             aTTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGtctc                    >  2:3092973/1‑149 (MQ=255)
                                                                                                                             aTTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGtctc                    >  2:3164464/1‑149 (MQ=255)
                                                                                                                              ttCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCa                   >  2:3009057/1‑149 (MQ=255)
                                                                                                                                cGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCAtt                 >  1:4048174/1‑149 (MQ=255)
                                                                                                                                 gATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTa                >  1:2618818/1‑149 (MQ=255)
                                                                                                                                  aTTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTAc               >  1:1067984/1‑149 (MQ=255)
                                                                                                                                  aTTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTAc               >  1:1355228/1‑149 (MQ=255)
                                                                                                                                  aTTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTAc               >  1:3120084/1‑149 (MQ=255)
                                                                                                                                  aTTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTAc               <  2:1609264/149‑1 (MQ=255)
                                                                                                                                   ttCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTAcc              >  2:1142539/1‑149 (MQ=255)
                                                                                                                                     cATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCgt            >  1:2207542/1‑149 (MQ=255)
                                                                                                                                      atatCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCgtg           >  2:3987498/1‑149 (MQ=255)
                                                                                                                                       tatCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCTTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCgtgt          >  1:2988825/1‑149 (MQ=255)
                                                                                                                                       tatCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCgtgt          >  2:632861/1‑149 (MQ=255)
                                                                                                                                        atCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTa         >  1:630075/1‑149 (MQ=255)
                                                                                                                                         tCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTAt        >  1:1591791/1‑149 (MQ=255)
                                                                                                                                          cGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTAtt       <  1:1948295/149‑1 (MQ=255)
                                                                                                                                           gCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTc      <  2:770697/149‑1 (MQ=255)
                                                                                                                                            cTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTcc     <  1:3973653/149‑1 (MQ=255)
                                                                                                                                              aTGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCg   <  2:1695960/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGC  >  CP009273/1820742‑1821033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑