Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,829,319 C→A 100% I85I (ATC→ATA)  ydjZ → TVP38/TMEM64 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,829,3190CA98.8% 779.6 / 0.3 241I85I (ATC→ATA) ydjZTVP38/TMEM64 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base A (137/100);  total (137/104)
Fisher's exact test for biased strand distribution p-value = 7.77e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 241 total aligned reads displayed.

ATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGC  >  CP009273/1829173‑1829464
                                                                                                                                                  |                                                                                                                                                 
  cAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCt                                                                                                                                               <  2:1973868/149‑1 (MQ=255)
  cAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCt                                                                                                                                               <  1:2412221/149‑1 (MQ=255)
    aTCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCttt                                                                                                                                             <  2:3813371/149‑1 (MQ=255)
     tCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTg                                                                                                                                            <  1:3873447/149‑1 (MQ=255)
     tCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTg                                                                                                                                            <  1:3874801/149‑1 (MQ=255)
     tCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTg                                                                                                                                            >  2:3571694/1‑149 (MQ=255)
      cGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGc                                                                                                                                           >  2:691444/1‑149 (MQ=255)
        cAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCa                                                                                                                                         <  1:3622083/149‑1 (MQ=255)
         aGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCaa                                                                                                                                        <  2:2715059/149‑1 (MQ=255)
            gTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGc                                                                                                                                     <  2:418639/149‑1 (MQ=255)
            gTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGc                                                                                                                                     <  1:2212809/149‑1 (MQ=255)
             ttGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTTCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAAACATTTCCAATGCg                                                                                                                                    >  1:2433160/1‑149 (MQ=255)
             ttGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCg                                                                                                                                    >  1:3072472/1‑149 (MQ=255)
              tGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGt                                                                                                                                   <  2:3676226/149‑1 (MQ=255)
                  gcgTTTGCCGCCGTGGACCTACAGGGTATAGAACGCTTAATTCAGTCTTACGGCGCACTGGGGGCAGTTGTCTCGTTCTTGTTGTTGATTTTGCAGGCCATAGCTGCACCGCTACCTGCGTTTTTGATATCGTTTGCCAATGCGTCggt                                                                                                                               <  2:3929514/149‑3 (MQ=255)
                  gcgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCt                                                                                                                               <  1:813043/149‑1 (MQ=255)
                   cgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTg                                                                                                                              <  1:3984241/149‑1 (MQ=255)
                    gTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGt                                                                                                                             <  1:1929255/149‑1 (MQ=255)
                     tttGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGtt                                                                                                                            <  2:522/149‑1 (MQ=255)
                      ttGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGttt                                                                                                                           <  2:3599041/149‑1 (MQ=255)
                            ccgTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGcgcg                                                                                                                     >  1:3705878/1‑149 (MQ=255)
                            ccgTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGcgcg                                                                                                                     >  1:1534124/1‑149 (MQ=255)
                            ccgTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGcgcg                                                                                                                     <  2:2167884/149‑1 (MQ=255)
                            ccgTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGcgcg                                                                                                                     >  2:210101/1‑149 (MQ=255)
                            ccgTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGcgcg                                                                                                                     >  2:208526/1‑149 (MQ=255)
                            ccgTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGcgcg                                                                                                                     >  2:1467840/1‑149 (MQ=255)
                             cgTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGt                                                                                                                    <  1:948484/149‑1 (MQ=255)
                              gTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGtt                                                                                                                   <  2:2504621/149‑1 (MQ=255)
                              gTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGtt                                                                                                                   >  2:1444958/1‑149 (MQ=255)
                               tGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTc                                                                                                                  >  2:717855/1‑149 (MQ=255)
                                ggACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCt                                                                                                                 >  1:1023620/1‑149 (MQ=255)
                                 gACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTg                                                                                                                >  1:1999620/1‑149 (MQ=255)
                                  aCCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTgg                                                                                                               >  1:2971917/1‑149 (MQ=255)
                                      aCAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGggcg                                                                                                           >  2:2907050/1‑149 (MQ=255)
                                        aGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGggcggc                                                                                                         >  2:1291893/1‑149 (MQ=255)
                                          ggTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCtt                                                                                                       >  1:1793736/1‑149 (MQ=255)
                                           gTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTg                                                                                                      >  1:2920698/1‑149 (MQ=255)
                                            tataGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGc                                                                                                     >  2:3040413/1‑149 (MQ=255)
                                              taGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTg                                                                                                   <  2:2664143/149‑1 (MQ=255)
                                               aGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTGTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGt                                                                                                  >  1:1611805/1‑149 (MQ=255)
                                                      tttATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGAc                                                                                           >  1:2356801/1‑149 (MQ=255)
                                                       ttATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGAGTTTGCAGGCCATTGCTGCACCGCTACCTGCGTGTTTGATAACCTTTGCCAATGCGTCGCTGTTAGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGAcc                                                                                          >  2:3118027/1‑149 (MQ=255)
                                                          ttCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGt                                                                                       >  1:1194999/1‑149 (MQ=255)
                                                          ttCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGt                                                                                       >  1:1944823/1‑149 (MQ=255)
                                                          ttCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGt                                                                                       >  2:1283579/1‑149 (MQ=255)
                                                           tCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGGTTGGCGCGTTCTGGGGCGGCTTGCGGTCGTGGTCCAGtt                                                                                      >  1:77367/1‑149 (MQ=255)
                                                            cAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTc                                                                                     <  2:2179469/149‑1 (MQ=255)
                                                            cAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTc                                                                                     <  2:178639/149‑1 (MQ=255)
                                                              gTCTTACGGCTCACTGGCGTCAGTTGTATCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCATCGCTACCTTCGTTTTTTATTACCTTTGACAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTTTCGTGGACCAGTTCGa                                                                                   <  2:950896/149‑1 (MQ=255)
                                                              gTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGa                                                                                   >  1:1516284/1‑149 (MQ=255)
                                                              gTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGa                                                                                   >  1:2828332/1‑149 (MQ=255)
                                                              gTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGa                                                                                   >  2:108789/1‑149 (MQ=255)
                                                              gTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGa                                                                                   <  1:1281067/149‑1 (MQ=255)
                                                               tCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGAt                                                                                  <  2:565479/149‑1 (MQ=255)
                                                                     ggCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcgg                                                                            >  2:1695383/1‑149 (MQ=255)
                                                                             ggcggcAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTTGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCggcgcggcgc                                                                    >  1:2090453/1‑149 (MQ=255)
                                                                             ggcggcAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCCCTTCGATGGCCcgcgcggcgc                                                                    >  1:2756787/1‑149 (MQ=255)
                                                                             ggcggcAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCggcgcggcgc                                                                    >  2:920595/1‑149 (MQ=255)
                                                                                   aGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCt                                                              >  1:3345286/1‑149 (MQ=255)
                                                                                    gTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCtt                                                             >  1:1715491/1‑149 (MQ=255)
                                                                                     ttGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCttt                                                            <  2:2271091/149‑1 (MQ=255)
                                                                                       gTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCttttt                                                          <  1:671544/149‑1 (MQ=255)
                                                                                       gTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCttttt                                                          <  2:495473/149‑1 (MQ=255)
                                                                                        tctcGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCtttttt                                                         >  2:2453285/1‑149 (MQ=255)
                                                                                            gTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTTGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCg                                                     >  2:2382366/1‑149 (MQ=255)
                                                                                            gTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCg                                                     >  2:2175023/1‑149 (MQ=255)
                                                                                                 tgttgATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCaga                                                <  2:1841782/149‑1 (MQ=255)
                                                                                                 tgttgATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCaga                                                <  2:2701510/149‑1 (MQ=255)
                                                                                                   ttgATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGt                                              <  1:3762385/149‑1 (MQ=255)
                                                                                                    tgatgaTTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGAACAGTTCGATGGCCGGCGCGGCGCTGTGCTATTTTATAGCCAGAGTg                                             >  1:2205803/1‑149 (MQ=255)
                                                                                                      atgatTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGAt                                           <  1:3571694/149‑1 (MQ=255)
                                                                                                       tgatTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATg                                          <  2:439267/149‑1 (MQ=255)
                                                                                                              gCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGGTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCCGCGTGATGGGCcgcg                                   >  1:59516/1‑149 (MQ=255)
                                                                                                                 ggCCATTGCTGCACCGATACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAg                                >  2:3012062/1‑149 (MQ=255)
                                                                                                                  gCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgt                               <  2:2920716/149‑1 (MQ=255)
                                                                                                                   ccATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGACgtg                              >  2:810974/1‑149 (MQ=255)
                                                                                                                   ccATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtg                              <  2:2793049/149‑1 (MQ=255)
                                                                                                                   ccATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtg                              <  2:1319542/149‑1 (MQ=255)
                                                                                                                   ccATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtg                              >  2:3640006/1‑149 (MQ=255)
                                                                                                                    cATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtgg                             <  1:2463971/149‑1 (MQ=255)
                                                                                                                     aTTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTTCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCGTGCTGGCGTTGACCAGTTAGATGGCCGGCGCGGCGCGGTGCTTTTTTATCGCCACAGTGAAGGGCCGCGAAgaggt                            >  1:2468336/1‑149 (MQ=255)
                                                                                                                     aTTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtggt                            >  2:1021949/1‑149 (MQ=255)
                                                                                                                      ttGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtggtg                           <  1:1417303/149‑1 (MQ=255)
                                                                                                                         ctgcACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGaa                        <  2:1602048/149‑1 (MQ=255)
                                                                                                                            cACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGaaaaa                     <  2:2813773/149‑1 (MQ=255)
                                                                                                                            cACCGATACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGaaaaa                     >  2:1076467/1‑149 (MQ=255)
                                                                                                                             aCCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGCAGTGGTGGAAAAAt                    >  1:1626758/1‑149 (MQ=255)
                                                                                                                              ccGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAAtt                   <  1:2710272/149‑1 (MQ=255)
                                                                                                                              ccGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAAtt                   >  1:1000811/1‑149 (MQ=255)
                                                                                                                                gCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTaa                 >  1:3259988/1‑149 (MQ=255)
                                                                                                                                 cTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAAc                <  2:1028910/149‑1 (MQ=255)
                                                                                                                                    ccTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCgg             >  2:3289210/1‑149 (MQ=255)
                                                                                                                                     cTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGc            >  2:3089316/1‑149 (MQ=255)
                                                                                                                                      tGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCa           >  1:4075705/1‑149 (MQ=255)
                                                                                                                                      tGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCa           >  1:3746616/1‑149 (MQ=255)
                                                                                                                                               gATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGc  <  1:4005706/149‑1 (MQ=255)
                                                                                                                                               gATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGc  >  2:3095987/1‑149 (MQ=255)
                                                                                                                                               gATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGc  <  2:2652933/149‑1 (MQ=255)
                                                                                                                                               gATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGc  <  2:2567499/149‑1 (MQ=255)
                                                                                                                                               gATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGc  <  2:587136/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGC  >  CP009273/1829173‑1829464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑